scholarly journals Assessment of the Value of Stomatal Size and Density as Hallmarks of Nonaploid Kiwifruit Plants

2021 ◽  
Vol 90 ◽  
Author(s):  
Iwona Chłosta ◽  
Grzegorz Góralski ◽  
Marzena Popielarska-Konieczna
Keyword(s):  
Genome Size ◽  
Morphological Characteristics ◽  
Diagnostic Feature ◽  
Actinidia Chinensis ◽  
Ploidy Levels ◽  
Stomatal Size ◽  
Length Width ◽  
Stomatal Length ◽  
Distinguishing Features

Abstract In vitro-derived plants with elevated ploidy levels can display distinguishing features from the plants they are derived from, especially owing to their indirect regeneration. Genome size affects cell size and, thus, affects plant morphological characteristics. Therefore, stomata traits may be used as a diagnostic feature allowing identification of ploidy. Actinidia chinensis var. deliciosa A. Chev. (A. Chev.), known as kiwifruit, is successfully cultured in vitro and redifferentiated into plants via endosperm-derived calli. To identify differences between hexaploids obtained from seeds and confirmed nonaploids obtained from endosperm-derived calli, we analyzed the stomata. Our results confirmed that ploidy coincides with mean stomatal length, width, and density. Despite this correlation, this method cannot be used to distinguish individual hexaploid kiwifruit plants from nonaploid ones because samples with different ploidy yielded overlapping measurements.

Genome size and nucleotypic variation in Malus germplasm

Genome ◽  
2009 ◽  
Vol 52 (2) ◽  
pp. 148-155 ◽  
Author(s):  
Schuyler S. Korban ◽  
Wannasiri Wannarat ◽  
Charlotte M. Rayburn ◽  
Tatiana C. Tatum ◽  
A. Lane Rayburn
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Internal Standard ◽  
The United States ◽  
Mean Value ◽  
Ploidy Levels ◽  
Flow Cytometric ◽  
Malus Species ◽  
Leaf Tissues ◽  
Stomatal Length

The genus Malus has anywhere between 25 and 33 species along with several subspecies. Malus species as well as clones within the same species have varying ploidy levels, as these are more than likely collected from different trees and (or) from different locations. In recent years, large numbers of Malus germplasm accessions have been collected and maintained at the United States National Germplasm Clonal Repository; however, genome sizes of this material have not yet been determined. In this study, leaf tissues from young grafted trees of 100 Malus species and hybrids growing in a nursery at the University of Illinois were collected and immediately used for extracting nuclei. Leaf tissues from apple and maize line W-22, used as an internal standard, were co-chopped and prepared for flow cytometric analysis. Apple nuclei were stained with propidium iodide, an intercalating dye, and a minimum of 8000 nuclei per sample were analyzed. Mean fluorescence of apple nuclei was then determined. A total of four replications per sample was used. Among 100 Malus accessions analyzed, one tetraploid, three triploid, and 96 diploid genotypes were identified. Significant differences in genome size were identified among the three ploidy types observed and also within diploid genotypes. The 2C mean value for tetraploids was 3.13 pg and ranged from 2.27 to 2.41 pg for triploids, whereas 2C values for diploids ranged between 1.44 and 1.72 pg. In addition, leaf impressions of young, fully expanded leaves were collected from young trees of 10 selected genotypes based on their ploidy and flow cytometric analysis and used to measure the nucleotypic parameter stomatal length. Ten stomata were measured per slide, three slides were analyzed per leaf, and three leaves were analyzed per accession. Overall, mean length of stomata ranged between 19.47 μm (diploid) and 27.6 μm (tetraploid), indicating that stomatal length in a tetraploid Malus genotype was 1.4-fold higher than that of a diploid genotype. A positive correlation between genome size and the nucleotypic parameter stomatal length was observed.

scholarly journals Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

2012 ◽  
Vol 29 (No. 1) ◽  
pp. 35-40 ◽  
Author(s):  
I. Ajalin ◽  
F. Kobza ◽  
J. Doležel
Keyword(s):  
Flow Cytometry ◽  
Leaf Blade ◽  
Morphological Characteristics ◽  
Colchicine Treatment ◽  
Ploidy Levels ◽  
Stomata Size ◽  
Secondary Screening ◽  
Length Width ◽  
Ploidy Identification ◽  
Index Value

The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola × wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM. 

scholarly journals In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

2020 ◽  
Vol 15 (1) ◽  
pp. 840-847
Author(s):  
Junjie Zhang ◽  
Ruiqi Pian ◽  
Endian Yang ◽  
Wei Zhou ◽  
Qian He ◽  
...  
Keyword(s):  
Moringa Oleifera ◽  
Agronomic Traits ◽  
Raw Materials ◽  
Morphological Characteristics ◽  
Ploidy Levels ◽  
Artificial Induction ◽  
Acid Detergent Fibre ◽  
Breeding Programmes ◽  
Drumstick Tree

AbstractArtificial induction of polyploidy is widely used in breeding programmes to improve the agronomic traits. The drumstick tree (Moringa oleifera Lam.) has a range of potential commercial uses, as the vegetative organs have high nutritional, medicinal, and feed values. In the present study, in vitro tetraploidisation in drumstick tree was performed by treating leaf segments with colchicine and subsequently verifying the ploidy levels. For polyploidisation, explant survival and regeneration rates were affected more by exposure time than by colchicine concentration, and the highest polyploidisation efficiency was observed at 500 mg/L colchicine for 3 days, which yielded 21% tetraploids. The morphological characteristics and contents of seven fodder-related nutrients (crude protein, ether extract, ash, acid detergent fibre, neutral detergent fibre, calcium, and phosphorus) in the leaves and shoots were compared between tetraploid and diploid drumstick trees. The resulting tetraploids showed significantly enhanced leaf and stomatal size. In addition, the contents of seven fodder-related nutrients were higher, although to varying degrees, in tetraploids than in diploids. The results indicated that the tetraploid produced in this study exhibited superior agronomical traits and improved biomass yield than diploids, and may represent excellent raw materials for fodder to enhance biomass and nutrition.

scholarly journals Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds

2017 ◽  
Vol 9 (3) ◽  
pp. 423
Author(s):  
Sri Indah Rahmawati ◽  
Ahmad Yunus ◽  
Ari Susilowati
Keyword(s):  
Artemisia Annua ◽  
Stomatal Density ◽  
Water Extract ◽  
Herbaceous Plant ◽  
Gloriosa Superba ◽  
Stomatal Size ◽  
Water Extracts ◽  
Water Solvent ◽  
Length Width ◽  
Stomatal Length

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> is a plant that contains high colchicine compounds, especially on the seeds. </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds was used as a mutagen for </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB">. The aim of this study was to determine the size and density of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> stomata soaked in water extract of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds as a mutagen. Extraction of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds and soaking time of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> sprouts. Measurements of stomatal length, width and density were conducted in epidermis of </span><em><span lang="EN-GB">Artemisia annua</span></em><span lang="EN-GB"> leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of </span><em><span lang="EN-GB">Gloriosa superba</span></em><span lang="EN-GB"> seeds proved the effects on stomatal size and density of treated plants.  </span></p>

scholarly journals Characterization and Control of Pestalotiopsis spp. the Causal Fungus of Guava Scabby Canker in El-Beheira Governorate, Egypt

2016 ◽  
Vol 4 (3) ◽  
pp. 121-136
Author(s):  
Eman El-argawy
Keyword(s):  
Disease Control ◽  
Morphological Characteristics ◽  
Pcr Analysis ◽  
Environment Impact ◽  
Safe Alternative ◽  
Growing Seasons ◽  
Length Width ◽  
Guava Leaves

During 2013-2014 growing seasons, forty three isolates of Pestalotiopsis spp. were recovered from guava leaves and fruits showed scab symptoms from different regions in EL-Beheira governorate. Five Pestalotiopsis species were recognized according to the morphological characteristics of fungal colony (Colony color, Size and number of acervulii) and conidia (length, width, and color of median cells, length and the number of apical and basal appendages); they were P. psidii, P. microspora, P. clavispora, P. neglecta and Pestalotiosis spp. All the isolates recovered were pathogenic to the cv. Balady of guava fruits. However, P.psidii isolates were the most highly pathogenic followed by P. neglecta, P. clavispora, P. microspora and Pestalotiopsis spp, respectively. RAPD-PCR analysis using five random oligonucleotide primers revealed DNA fingerprints and considerable variations were revealed with primers tested.  Bar primer showed a common band for all Pestalotiopsis isolates and species at 500bp, while BAQ, 18 and A9B4 exhibited banding pattern similar for all isolates of the same species which were different from that of the other species. Scab disease control of infected fruits by chitosan as a natural product was tested. The in vitro 2.5% chitosan application significantly inhibited the growth of Pestalotiopsis spp. tested by 86.53% on agar plates. The in vivo tests on fruits, the chitosan treatment to artificially infected fruits reduced the development of symptoms at the different chitosan concentrations, i.e., 1.5%, 2% and 2.5%. The 2.5% chitosan was the most effective concentration for scab disease control in guava fruits. It is the first report of identification five different Pestalotiopsis  species affecting guava fruits and leaves in EL-Beheira Governorate, Egypt. Also, the study supported the view that chitosan offers a safe alternative to synthetic fungicides in postharvest scabby control and could be considered as a potential agrochemical of low environment impact.

The experience of holding the cycles of assisted reproductive technology with defrost, a biopsy, genetic study and refreezing of embryos in patients with multiple unsuccessful implantations

2016 ◽  
pp. 166-170
Author(s):  
Y.V. Masliy ◽  
◽  
I.O. Sudoma ◽  
P.S. Mazur ◽  
D.A. Mykytenko ◽  
...  
Keyword(s):  
Chromosomal Rearrangements ◽  
Genetic Diagnosis ◽  
Morphological Characteristics ◽  
Implantation Rate ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Comparative Genome Hybridization ◽  
Comparative Genome ◽  
Vitro Fertilization

The objective: to study the possibility of using frozen blastocysts for biopsy and genetic testing and performance measurement transfer euploeded 5–7-day-old embryos after thawing, biopsies, refreezing and thawing in patients with unsuccessful implantation. Patients and methods. The object of the study was the group of patients with repeated failure of implantation (4) in programs of auxiliary reproductive technologies (ART), subject to transfer to the uterus in total (i.e. in all the programs) for at least 6 good quality embryos based on morphological characteristics). All women had sufficient ovarian reserve. The patient was treated for infertility within the ART programs of the clinic of reproductive medicine "Nadiya" in the period from 2006 to 2016. The sample included couples who were not carriers of chromosomal rearrangements, without anomalies of the uterus (congenital and acquired: a doubling of the uterus, one-horned uterus, intrauterine membrane, synechia, submucous myoma of the uterus). All women had a positive ovarian response to controlled stimulation with gonadotropins (at least 7 oocytes) and a sufficient number of cryopreserved embryos. The first group (G1) included 64 women who trophectodermal a biopsy was performed on fresh blastocysts (in a loop controlled ovarian hyperstimulation). The second group (G2) were included 31 women who underwent thawing previously cryopreserved blastocysts trophectodermal re-biopsy and vitrification of blastocysts. Results. It was found that the performance of transfers euploid embryos that were vitrified, bioptrone and revitriphted, a little lower than those that were bioptrone fresh and vitrified only once. At the same time computationa genetic diagnosis previously vitrified blastocysts using comparative genome hybridization in patients with recurrent failed implantation allows to obtain a reasonable pregnancy rate (58%), implantation rate (33.3 %) and the birth of living children (45.1 %). Conclusion. Reprising biopropane embryos does not cause significant destructive impact and allows you to achieve pregnancy and birth of the alive child. Key words: in vitro fertilization, reusable unsuccessful implantation, a method of comparative genome hybridization, refreezing.

Formulation of Modified Release Atorvastatin Nanoparticles by Emulsion Interfacial Reaction Method

2015 ◽  
Vol 8 (3) ◽  
pp. 2937-2940
Author(s):  
Sudhakar Sekar ◽  
Shee Sim May
Keyword(s):  
Interfacial Reaction ◽  
Therapeutic Potential ◽  
Chitosan Nanoparticles ◽  
In Vitro Release ◽  
Morphological Characteristics ◽  
Preparation Technique ◽  
Modified Release ◽  
Reaction Method ◽  
Vitro Release

The aim of the study is to formulate a modified release chitosan nanoparticles for the oral delivery of atorvastatin and to study the in vitro release of atorvastatin from chitosan nanoparticles. Atorvastatin-loaded chitosan nanoparticles were prepared with different concentration of cross-linking agent (glutaraldehyde) by emulsion interfacial reaction method. The formed nanoparticles were characterized in terms of size and morphological characteristics by scanning electron microscopy (SEM) and transmission electron microscope (TEM). Spherical and regular nanoparticles with the size range of 100-250nm were formed. Atorvastatin encapsulation efficiency of nanoparticles was found to be highest in ANP3, followed by ANP2 and ANP1. The in vitro release of atorvastatin was studied by membrane diffusion technique. The resulted cumulative percentage of drug released for ANP1, ANP2 and ANP3 were 60.08%, 34.81% and 20.39% respectively. Through this study, the nanoparticles preparation technique has shown to be a promising approach for enhancing the dissolution of hydrophobic drugs like atorvastatin calcium. The application of this novel delivery system offers good therapeutic potential in the management of hypercholesterolemia and dyslipidemia.

scholarly journals Cytotype distribution and chloroplast phylogeography of the Actinidia chinensis complex

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhi Wang ◽  
Caihong Zhong ◽  
Dawei Li ◽  
Chunlin Yan ◽  
Xiaohong Yao ◽  
...  
Keyword(s):  
Environmental Changes ◽  
Demographic History ◽  
Wide Distribution ◽  
Historical Demography ◽  
Actinidia Chinensis ◽  
Ecological Niche Models ◽  
Subtropical China ◽  
Modeling Tools ◽  
Climatic Fluctuations ◽  
Ploidy Levels

Abstract Background Plant phylogeographic studies of species in subtropical China have mainly focused on rare and endangered species, whereas few studies have been conducted on taxa with relatively wide distribution, especially polyploid species. We investigated the cytotype and haplotype distribution pattern of the Actinidia chinensis complex, a widespread geographically woody liana with variable ploidy in subtropical China comprising two varieties, with three chloroplast fragments DNA (ndhF-rpl132, rps16-trnQ and trnE-trnT). Macroevolutionary, microevolutionary and niche modeling tools were also combined to disentangle the origin and the demographic history of the species or cytotypes. Results The ploidy levels of 3338 individuals from 128 populations sampled throughout the species distribution range were estimated with flow cytometry. The widespread cytotypes were diploids followed by tetraploids and hexaploids, whereas triploids and octoploids occurred in a few populations. Thirty-one chloroplast haplotypes were detected. The genetic diversity and genetic structure were found to be high between varieties (or ploidy races) chinensis and deliciosa. Our results revealed that these two varieties inhabit significantly different climatic niche spaces. Ecological niche models (ENMs) indicate that all varieties’ ranges contracted during the Last Inter Glacial (LIG), and expanded eastward or northward during the Last Glacial Maximum (LGM). Conclusions Pliocene and Plio-Pleistocene climatic fluctuations and vicariance appear to have played key roles in shaping current population structure and historical demography in the A. chinensis complex. The polyploidization process also appears to have played an important role in the historical demography of the complex through improving their adaptability to environmental changes.

scholarly journals Immunocompatibility of a new dual modality contrast agent based on radiolabeled iron-oxide nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria-Argyro Karageorgou ◽  
Dimosthenis Stamopoulos
Keyword(s):  
Complete Blood Count ◽  
Morphological Characteristics ◽  
Mononuclear Phagocyte ◽  
Dual Modality ◽  
Immunodeficient Mice ◽  
Force Microscopy ◽  
Magnetic Resonance Imaging Mri ◽  
Diagnostic Applications

AbstractRadiolabeled magnetic nanoparticles are promising candidates as dual-modality-contrast-agents (DMCA) for diagnostic applications. The immunocompatibility of a new DMCA is a prerequisite for subsequent in vivo applications. Here, a new DMCA, namely Fe3O4 nanoparticles radiolabeled with 68Ga, is subjected to immunocompatibility tests both in vitro and in vivo. The in vitro immunocompatibility of the DMCA relied on incubation with donated human WBCs and PLTs (five healthy individuals). Optical microscopy (OM) and atomic force microscopy (AFM) were employed for the investigation of the morphological characteristics of WBCs and PLTs. A standard hematology analyzer (HA) provided information on complete blood count. The in vivo immunocompatibility of the DMCA was assessed through its biodistribution among the basic organs of the mononuclear phagocyte system in normal and immunodeficient mice (nine in each group). In addition, Magnetic Resonance Imaging (MRI) data were acquired in normal mice (three). The combined OM, AFM and HA in vitro data showed that although the DMCA promoted noticeable activation of WBCs and PLTs, neither degradation nor clustering were observed. The in vivo data showed no difference of the DMCA biodistribution between the normal and immunodeficient mice, while the MRI data prove the efficacy of the particular DMCA when compared to the non-radiolabeled, parent CA. The combined in vitro and in vivo data prove that the particular DMCA is a promising candidate for future in vivo applications.

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