scholarly journals Ectopic expression of secretory peptide PdEPF3 in Arabidopsis confers drought tolerance with reduced stomatal density

2019 ◽  
Vol 88 (2) ◽  
Author(s):  
Sha Liu ◽  
Fuli Jia ◽  
Zhiyin Jiao ◽  
Jingjing Wang ◽  
Xinli Xia ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Drought Tolerance ◽  
Populus Deltoides ◽  
Stomatal Density ◽  
Ectopic Expression ◽  
Populus Nigra ◽  
Primary Roots ◽  
Transgenic Breeding ◽  
Use Efficiency ◽  
Seedling Germination

Drought is one of the most prevalent environmental stresses that affect plant growth and development. Improvement in drought tolerance is associated with reduced stomatal density and higher water use efficiency (WUE). In this study, an epidermal patterning factor (EPF), PdEPF3, from a fast-growing poplar clone, NE-19 [<em>Populus nigra</em> × (<em>Populus deltoides</em> × <em>Populus nigra</em>)], was characterized. Quantitative reverse transcription polymerase chain reaction showed that the transcription of <em>PdEPF3</em> was induced by drought. We further found that the transgenic <em>Arabidopsis</em> overexpressing <em>PdEPF3</em> had an earlier seedling germination and longer primary roots under osmotic stress treatments, compared with the WT and mutant <em>epf1-1</em>. In addition, ectopic overexpression of <em>PdEPF3</em> in <em>Arabidopsis</em> plants was able to enhance drought tolerance. This observation was associated with the reduced stomatal density of transgenic plants, which would limit transpiration and reduce water loss, consequently improving the WUE of plants. Interestingly, the reduction of stomatal density in transgenic plants overexpressing <em>PdEPF3</em> did not affect their photosynthetic capacity. These results indicate that <em>PdEPF3</em> could be used in transgenic breeding to enhance plant drought tolerance.

scholarly journals The Receptor-Like Kinase ERECTA Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density

2021 ◽  
Vol 22 (14) ◽  
pp. 7245
Author(s):  
Huiguang Li ◽  
Yanli Yang ◽  
Houling Wang ◽  
Sha Liu ◽  
Fuli Jia ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Populus Deltoides ◽  
Stomatal Density ◽  
Populus Nigra ◽  
35S Promoter ◽  
Control Line ◽  
Stomatal Size ◽  
Use Efficiency

Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the PdERECTA gene, which is a member of the LRR-RLKs family from Populus nigra × (Populus deltoides × Populus nigra), improves water use efficiency and enhances drought tolerance in triploid white poplar. PdERECTA localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24–34% lower and the stomatal size was 12–14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18–35% lower than the control line, and instantaneous water use efficiency was around 14–63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. PdERECTA overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that PdERECTA can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes PdERECTA trees promising candidates for establishing more water use efficient plantations.

scholarly journals The Arabidopsis GTL1 Transcription Factor Regulates Water Use Efficiency and Drought Tolerance by Modulating Stomatal Density via Transrepression of SDD1

2010 ◽  
Vol 22 (12) ◽  
pp. 4128-4141 ◽  
Author(s):  
Chan Yul Yoo ◽  
Heather E. Pence ◽  
Jing Bo Jin ◽  
Kenji Miura ◽  
Michael J. Gosney ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Stomatal Density ◽  
Use Efficiency

scholarly journals Emerging Molecular Strategies for Improving Rice Drought Tolerance

2020 ◽  
Vol 21 ◽  
Author(s):  
Jitender Giri ◽  
Swarup K. Parida ◽  
Saurabh Raghuvanshi ◽  
Akhilesh K. Tyagi
Keyword(s):  
Drought Tolerance ◽  
Protein Modification ◽  
Stomatal Density ◽  
Drought Response ◽  
Food Crop ◽  
High Demand ◽  
Rice Varieties ◽  
Drought Tolerant ◽  
The World ◽  
Use Efficiency

Abstract:: Rice occupies a pre-eminent position as a food crop in the world. Its production, however, entails up to 3000 liters of water per kilogram of grain produced. Such high demand makes rice prone to drought easily. Sustainable rice cultivation with limited water resources requires the deployment of a suitable strategy for better water use efficiency and improved drought tolerance. Several drought-related genes have been evaluated in rice for their mode of action in conferring drought tolerance. Manipulation of components of abscisic acid signal transduction, stomatal density, deposition of cuticular wax, and protein modification pathways are emerging as priority targets. Gene reprogramming by microRNAs is also being explored to achieve drought tolerance. Genetically dissected Quantitative Trait Loci (QTLs) and their constituent genes are being deployed to develop drought-tolerant rice varieties. Progressive research and challenges include a better understanding of crucial components of drought response and search for new targets, along with the deployment of improved varieties in the field.

scholarly journals Heterologous Expression of Arabidopsis thaliana rty Gene in Strawberry (Fragaria × ananassa Duch.) Improves Drought Tolerance

2020 ◽  
Author(s):  
Maofu Li ◽  
Yuan Yang ◽  
Ali Raza ◽  
Shanshan Yin ◽  
Hua Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Heterologous Expression ◽  
Water Use Efficiency ◽  
Water Use ◽  
Water Loss ◽  
Loss Rate ◽  
Water Loss Rate ◽  
Use Efficiency

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferaseⅡ(NPTⅡ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

scholarly journals Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

2020 ◽  
Author(s):  
Maofu Li ◽  
Yuan Yang ◽  
Ali Raza ◽  
Shanshan Yin ◽  
Hua Wang ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Drought Tolerance ◽  
Heterologous Expression ◽  
Water Use ◽  
Water Loss ◽  
Loss Rate ◽  
Stomatal Closure ◽  
Fragaria Ananassa ◽  
Water Loss Rate ◽  
Use Efficiency

Abstract Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutation of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels and roots and shoot growth, but the effects of this gene in strawberry remain unclear. Results Here, we heterologously expressed Arabidopsis rty in strawberry plants and examined the effects of rty expression on the hormonal and physiological properties of the plants. Heterologous expression of rty induced IAA accumulation and increased the production of adventitious roots as well as trichomes on the abaxial leaf surface of the transgenic plants. Furthermore, the transgenic strawberry plants had increased ABA accumulation and stomatal closure. The transgenic strawberry plants exhibited enhanced water use efficiency and a reduced water loss rate. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the untransformed controls, and the transgenic plants were more drought tolerant than the wild-type plants. Our results uncover a transgenic approaches can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions. Conclusions In this study, the rty gene improves hormone-mediated drought tolerance in transgenic strawberry. We demonstrated that the heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. This study provides the basis for future genetic modifications of strawberry to improve drought tolerance.

Pu-miR172d regulates stomatal density and water use efficiency via targeting PuGTL1 in poplar

2020 ◽  
Author(s):  
Quangang Liu ◽  
Zhanchao Wang ◽  
Sen Yu ◽  
Wenlong Li ◽  
Mengqiu Zhang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Stomatal Density ◽  
Negative Regulator ◽  
Stomatal Development ◽  
Rna Seq ◽  
Mature Leaves ◽  
Gus Reporter ◽  
Young Leaves ◽  
Use Efficiency ◽  
Major Target

Abstract miRNAs play essential regulatory roles in many aspects of plant development and responses to abiotic and biotic environments. Here, we characterized Pu-miR172d, which acts as a negative regulator of stomatal density by directly repressing the expression of PuGTL1 in Populus ussuriensis. quantitative real-time PCR (RT-qPCR) and GUS reporter analyses showed that Pu-miR172d was strongly expressed in the guard cells of young leaves. Pu-miR172d overexpression significantly decreased stomatal density, resulting in the increase of WUE and drought tolerance by reducing net photosynthetic rate, stomatal conductance, and transpiration. Molecular analysis showed that PuGTL1 was a major target of Pu-miR172d cleavage. Moreover, PuGTL1-SRDX plants, in which PuGTL1 is suppressed, phenocopied Pu-miR172d overexpression lines with reduced stomatal density and enhanced WUE. In addition, the expression level of PuSDD1, a negative regulator of stomatal development, was significantly increased in young leaves of both Pu-miR172d overexpression and PuGTL1-SRDX plants. RNA-seq analysis of mature leaves revealed that Pu-miR172d overexpression decreased the expression of many genes related to photosynthesis. Our findings show that the Pu-miR172d/PuGTL1/PuSDD1 module plays an important role in stomatal differentiation, indicating its ability to improve the engineering of drought tolerance in poplar.

Controlling water use efficiency and drought tolerance through the GTL1-SDD1 stomatal density switch

2014 ◽  
Author(s):  
Hillel Fromm ◽  
Michael V. Mickelbart ◽  
Yaakov Tadmor ◽  
Paul Michael Hasegawa
Keyword(s):  
Drought Tolerance ◽  
Water Use Efficiency ◽  
Water Use ◽  
Stomatal Density ◽  
Use Efficiency

scholarly journals Heterologous Expression of Arabidopsis thaliana rty Gene in Strawberry (Fragaria × ananassa Duch.) Improves Drought Tolerance

2021 ◽  
Author(s):  
Maofu Li ◽  
Yuan Yang ◽  
Ali Raza ◽  
Shanshan Yin ◽  
Hua Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Heterologous Expression ◽  
Water Use Efficiency ◽  
Water Use ◽  
Water Loss ◽  
Loss Rate ◽  
Water Loss Rate ◽  
Use Efficiency

Abstract Background: Strawberry (Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry. Results: rty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase Ⅱ (NPT Ⅱ). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5 ng g–1 and 66.0 ng g–1in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3 ng g–1 and in transgenic plants were 543.8 ng g–1. The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses. Conclusions: The rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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