scholarly journals The assessment of incompatibility and cross-compatibility in three populations of European globeflower (Trollius europaeus L.) by observing the pollen tubes growth

2017 ◽  
Vol 86 (2) ◽  
Author(s):  
Wojciech Antkowiak ◽  
Janetta Niemann ◽  
Andrzej Wojciechowski
Keyword(s):  
Natural Populations ◽  
Pollen Grains ◽  
Diallel Cross ◽  
Pollen Tubes ◽  
Small Degree ◽  
Trollius Europaeus ◽  
Cross Pollination ◽  
Cross Compatibility ◽  
Two Populations ◽  
Made In

<span>The European globeflower was shown to be highly self‐incompatible by flower bagging experiments, however, a very small degree of selfing was observed in natural populations. The present study is an attempt to evaluate the level of self-incompatibility (SI) in three <em>Trollius europaeus</em> populations from different sites of Poland and to assess a degree of cross-compatibility (CC) between these populations. The SI and CC of the globeflower populations were evaluated based on the pollen germination index (PGI). The observations of pollen grains germination and pollen tubes penetration were made in pistils after self- and diallel cross-pollination of globeflower plants. The pollination combinations which had the PGI equal or higher than 2 were regarded as compatible. Generally, the PGI after self-pollination of all globeflower population was over 2, indicating that they are self-compatible. Also, in two globeflower populations after cross-pollination the PGI was higher than 2 showing that there was cross-compatibility between plants of these two populations. However, the third population, from Biedrusko, was fully cross-incompatible.</span>

scholarly journals Reproductive Output and Insect Behavior in Hybrids and Apomicts from Limonium ovalifolium and L. binervosum Complexes (Plumbaginaceae) in an Open Cross-Pollination Experiment

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 169
Author(s):  
Sofia I. R. Conceição ◽  
Joana Fernandes ◽  
Elsa Borges da Silva ◽  
Ana D. Caperta
Keyword(s):  
Reproductive Output ◽  
Natural Populations ◽  
Diploid Species ◽  
Pollen Grains ◽  
Insect Behavior ◽  
Reproductive Barrier ◽  
Ex Situ ◽  
Cross Pollination ◽  
Plant Collections ◽  
Fertile Hybrids

Ex situ plant collections established from seeds of natural populations are key tools for understanding mating systems of intricate taxonomic complexes, as in the Limonium Mill. genus (sea lavenders, Plumbaginaceae). Plants show a polymorphic sexual system associated to flower polymorphisms such as ancillary pollen and stigma and/or heterostyly that prevents self and intramorph mating. The main objectives of this study were to investigate the significance of pollen-stigma dimorphisms and the role of flower visitors in the reproductive output of hybrids arising from sexual diploids of Limonium ovalifolium complex and apomicts tetraploids of L. binervosum complex in an open cross-pollination experiment. Results showed that, similarly to parental plants, hybrids present inflorescence types, self-incompatible flowers, and produced regular pollen grains with the typical exine patterns, with medium to high viability. By contrast, apomicts show floral polymorphisms, inflorescences, and pollen grains of maternal phenotype but with low stainability. Several insects’ species visited the inflorescences of parental plants and both hybrids and apomicts and some of these insects carried A and/or B pollen grains on their bodies, especially Clepsis coriacana (Rebel) and Tapinoma sp. Insects’ floral visits to hybrids and apomicts seem to be independent of pollen fertility and plants’ reproductive modes. Both hybrids and apomicts were able to produce fertile seeds, although the latter showed more seedlings with developmental anomalies than the first plants. The findings demonstrate that there is a weak reproductive barrier between the diploid species of L. ovalifolium complex as they can hybridize and produce fertile hybrids, provided there is pollen transport by pollinator insects. This study supports that apomixis is a strong reproductive barrier between both L. ovalifolium and L. binervosum complexes but did not allow us to exclude reproductive interferences of apomict pollen into sexuals.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Immunolocalization of H+-ATPases in the plasma membrane of pollen grains and pollen tubes ofLilium longiflorum

PROTOPLASMA ◽  
1992 ◽  
Vol 171 (1-2) ◽  
pp. 55-63 ◽  
Author(s):  
G. Obermeyer ◽  
M. L�tzelschwab ◽  
H. -G. Heumann ◽  
M. H. Weisenseel
Keyword(s):  
Plasma Membrane ◽  
Pollen Grains ◽  
Pollen Tubes

FUNGI IN AIR MASSES OVER MONTREAL DURING 1950 AND 1951

1956 ◽  
Vol 34 (1) ◽  
pp. 1-15 ◽  
Author(s):  
S. M. Pady ◽  
L. Kapica
Keyword(s):  
Seasonal Variation ◽  
Pollen Grains ◽  
Average Concentration ◽  
Yeast Cells ◽  
The Sun ◽  
Air Masses ◽  
Air Sampler ◽  
Agricultural Areas ◽  
Very High ◽  
Made In

Numbers and kinds of fungi were determined from nutrient plate and silicone slide studies from the roof of the Sun Life Building, Montreal, between September 1950 and December 1951. Exposures of plates were made in the General Electric Bacterial Air Sampler, and plates and silicone slides in the Bourdillon Slit Sampler. A total of 978 exposures was made on 113 sampling days during 16 months; 507 plates in the G. E. Sampler, 344 plates and 127 slides in the Slit Sampler. Of 40,359 colonies examined, Cladosporium, Penicillium, yeasts, Aspergillus, Alternaria, and Actinomycetes were commonest, constituting 47.7, 15.8, 10.4, 4.6, 4.2, and 2.2% of the total. The next commonest fungi were Pullularia, Oöspora, Fusarium, Stemphylium, Verticillium, Rhizopus, Spicaria, Scopulariopsis, Phoma, Mucor, Botrytis, Cephalosporium, Trichoderma, Helmin-thosporium, Neurospora, Papularia, Cephalothecium, Pyrenochaeta, Zythia, and Nigrospora. In addition 12 genera were infrequently found. Unidentified colonies numbered 174 and nonsporulating 3371 (8.3%). On a cubic foot basis numbers in the plates varied from 17.7 per cu. ft. in August to 0.4 per cu. ft in February.Fungus spores showed a seasonal variation with summer highs averaging 244 per cu. ft. in July to a low of 0.8 per cu. ft. in December. The most abundant spores were Cladosporium, yeasts, smuts, Fusarium, Alternaria, Venturia-like, Stemphylium, rusts, Septoria, and Helminthosporium. Hyphal fragments and pollen grains were present also. On eight occasions during the summer, readings of over 200 spores per cu. ft. were recorded, the maximum being 445 per cu. ft. on September 6, 1951. Cladosporium in August reached a peak of 74.1 per cu. ft. and yeast cells in July had an average concentration of 100 per cu. ft.An analysis of the air masses indicated that pure polar air carried low numbers of fungi, whereas tropical air had very high numbers. Most of the air masses were modified polar air and their fungus content varied considerably. The fungi in the air over Montreal are believed to have had their origin in agricultural areas.

scholarly journals Interspecific Hybridization Processes between Michelia yunnanensis and M. crassipes and Embryogenesis of the Heterozygote

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1043-1047
Author(s):  
Haiyan Xu ◽  
Folian Li ◽  
Yuezhi Pan ◽  
Xun Gong
Keyword(s):  
Reciprocal Cross ◽  
Embryo Sac ◽  
Pollen Grains ◽  
Blue Staining ◽  
Formation Processes ◽  
Callose Deposition ◽  
Cross Compatibility ◽  
Direct Cross ◽  
Hybrid Seeds ◽  
Aniline Blue Staining

The investigation of hybridization processes and embryogenesis of heterozygote is an effective approach for early hybrids’ identification, which could provide reliable information for successful crossbreeding. In this study, we reported the whole hybridization processes of the direct cross and reciprocal cross between Michelia yunnanensis Franch. ex Finet et Gagnep. and Michelia crassipes Law using fluorescence microscopy after aniline blue staining, with the pollen germination on stigmas, pollen tube growth in styles, and subsequent extension into the embryo sac as well as the double fertilization processes are documented in detail. The M. yunnanensis × M. crassipes combination displayed considerable cross-compatibility, and the heterozygote embryogenesis was further observed with an approach of modified cryosectioning technique. Besides, the whole formation processes of hybrid seeds from artificial pollination to maturation were successfully observed. However, in the reciprocal cross, we found incompatibility between pollen grains of M. yunnanensis and stigmas of M. crassipes for the reason of hysteretic identification, as well as the abnormal callose deposition which belongs to the prefertilization barriers. This is the first study in which the complete and clear hybridization processes in Michelia were reported. We inferred that unilateral incompatibility of M. crassipes detected in this study may also exist in some other Michelia species. In artificial hybridization practices, we suggest some special treatments for overcoming prefertilization barrier should be taken when treating M. crassipes as the maternal parent.

Psychiatry in the Northern Sudan: A Study in Comparative Psychiatry

1968 ◽  
Vol 114 (513) ◽  
pp. 945-948 ◽  
Author(s):  
M. E. Elsarrag
Keyword(s):  
Private Practice ◽  
Psychiatric Illness ◽  
The West ◽  
Government Clinic ◽  
The Government ◽  
Two Populations ◽  
Made In

This study is mainly based on my work in psychiatry in the Clinic of Nervous Disorders, Khartoum North, and with my private practice patients. I have seen altogether 2,160 patients. The distribution of psychiatric illness in these is shown in the table below. The two populations of patients attending the Government clinic and private practice are comparable, and the same patients interchange. A comparison is made in this paper between psychiatry in the West, mainly British psychiatry, and Sudanese psychiatry.

scholarly journals Prunus necrotic ringspot virus Early Invasion and Its Effects on Apricot Pollen Grain Performance

2007 ◽  
Vol 97 (8) ◽  
pp. 892-899 ◽  
Author(s):  
Khalid Amari ◽  
Lorenzo Burgos ◽  
Vicente Pallas ◽  
María Amelia Sanchez-Pina
Keyword(s):  
Developmental Stages ◽  
Generative Cell ◽  
Pollen Grains ◽  
Growth Zone ◽  
Pollen Tubes ◽  
Climatic Conditions ◽  
Pollen Grain ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus

The route of infection and the pattern of distribution of Prunus necrotic ringspot virus (PNRSV) in apricot pollen were studied. PNRSV was detected both within and on the surface of infected pollen grains. The virus invaded pollen during its early developmental stages, being detected in pollen mother cells. It was distributed uniformly within the cytoplasm of uni- and bicellular pollen grains and infected the generative cell. In mature pollen grains, characterized by their triangular shape, the virus was located mainly at the apertures, suggesting that PNRSV distribution follows the same pattern as the cellular components required for pollen tube germination and cell wall tube synthesis. PNRSV also was localized inside pollen tubes, especially in the growth zone. In vitro experiments demonstrated that infection with PNRSV decreases the germination percentage of pollen grains by more than half and delays the growth of pollen tubes by ≈24 h. However, although PNRSV infection affected apricot pollen grain performance during germination, the presence of the virus did not completely prevent fertilization, because the infected apricot pollen tubes, once germinated, were able to reach the apricot embryo sacs, which, in the climatic conditions of southeastern Spain, mature later than in other climates. Thus, infected pollen still could play an important role in the vertical transmission of PNRSV in apricot.

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