scholarly journals Free and bound phenolic acids in inflorescences and rhizomes with roots of Hieracium pilosella L. (Asteraceae)

2011 ◽  
Vol 75 (3) ◽  
pp. 215-218
Author(s):  
Tadeusz Krzaczek ◽  
Monika Gawrońska-Grzywacz
Keyword(s):  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Plant Materials ◽  
Hieracium Pilosella ◽  
Rp Hplc ◽  
Medicinal Properties

The analysis of the fractions of free and bound, liberated by hydrolysis, phenolic acids in the inflorescences and rhizomes with roots of <em>Hieracium pilosella</em> L. was conducted by 2D-TLC and RP-HPLC methods. In the inflorescences fourteen phenolic acids were found: chlorogenic, gallic, protocatechuic, homoprotocatechuic, caffeic, gentisic, p-hydroxyphenylacetic, p-hydroxybenzoic, m-hydroxybenzoic, p-coumaric, syringic, vanillic, ferulic and salicylic. In the rhizomes with roots nine phenolic acids were identified: chlorogenic, gallic, homoprotocatechuic, caffeic, gentisic, p- hydroxyphenylacetic, p-hydroxybenzoic, syringic and vanillic. By means of the RP-HPLC method the contents of major acids were estimated which ranged from 0.01 mg% up to 139.50 mg%. Caffeic acid is the remarkably dominating constituent of both plant materials. Phenolic compounds may contribute to invasiveness and medicinal properties of this species.

scholarly journals Anti-diabetes Constituents in Leaves of Smallanthus sonchifolius

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Xiang Zheng ◽  
He Fan ◽  
Kang Ting-Guo ◽  
Dou De-Qiang ◽  
Gai Kuo ◽  
...  
Keyword(s):  
Quality Control ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Analytical Method ◽  
Inhibitory Effect ◽  
Hplc Method ◽  
Smallanthus Sonchifolius ◽  
Rp Hplc

The inhibitory effect of smallanthaditerpenic acids A, B, C and D previously isolated from leaves of Smallanthus sonchifolius (yacon) on α-glucosidase were examined and their IC50 were determined to be 0.48 mg/mL, 0.59 mg/mL, 1.00 mg/mL, and 1.17 mg/mL respectively. In addition, a rapid, reliable RP-HPLC method for the analysis of chlorogenic acid, caffeic acid, and smallanthaditerpenic acids A and C in yacon leaves was established, and the variation in their contents in leaves from plants cultivated in different places and collected at different times of the year were compared. The established analytical method for determining smallanthaditerpenic acids A and C, chlorogenic acid and caffeic acid presented good results and could be used as a method for the quality control of S. sonchifolius leaves.

scholarly journals The effects of plant density and irrigation on phenolic content in cauliflower 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 178-185 ◽  
Author(s):  
Alina Kałużewicz ◽  
Jolanta Lisiecka ◽  
Monika Gąsecka ◽  
Włodzimierz Krzesiński ◽  
Tomasz Spiżewski ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Plant Density ◽  
Phenolic Content ◽  
Sinapic Acid ◽  
Dry Weight ◽  
Coumaric Acid

This study was conducted to study the influence of plant density and irrigation on the content of phenolic compounds, i.e., phenolic acids and flavonols in cv. ‘Sevilla’ cauliflower curds. Levels of phenolic acids and flavonols were in the range of 3.0–6.2 mg and 25.4–87.8 mg/100 g of dry weight, respectively, depending on plant density and irrigation. Of the phenolic acids, caffeic acid was detected in the highest amount, followed by p-coumaric acid, sinapic acid, gallic acid, and ferulic acid. Of the two flavonols detected, the levels of quercetin were higher than those of kaempferol. The content of the detected phenolic acids (with the exception of ferulic acid) and both flavonols increased with increasing plant density. Furthermore, the concentration of phenolic compounds (with the exception of ferulic acid) was significantly higher under irrigation.

scholarly journals RP-HPLC Analysis of Phenolic Acids of Selected Central European Carex L. (Cyperaceae) Species and Its Implication for Taxonomy

2011 ◽  
Vol 94 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Anna Bogucka-Kocka ◽  
Katarzyna Szewczyk ◽  
Magdalena Janyszek ◽  
Sławomir Janyszek ◽  
Łukasz Cieśla
Keyword(s):  
Cluster Analysis ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Hplc Analysis ◽  
Sinapic Acid ◽  
Plant Tissues ◽  
Coumaric Acid ◽  
Central European ◽  
The Real ◽  
Rp Hplc

Abstract Eighteen species belonging to the Carex genus were checked for the presence and the amount of eight phenolic acids (p-hydroxybenzoic, vanillic, caffeic, syringic, protocatechuic, p-coumaric, sinapic, and ferulic) by means of HPLC. Both the free and bonded phenolic acids were analyzed. The majority of the analyzed acids occurred in the studied species in relatively high amounts. The highest concentrations found were caffeic acid and p-coumaric acid, for which the detected levels were negatively correlated. A very interesting feature was the occurrence of sinapic acid, a compound very rarely detected in plant tissues. Its distribution across the analyzed set of species can be hypothetically connected with the humidity of plants' habitats. Several attempted tests of aggregative cluster analysis showed no similarity to the real taxonomical structure of the genus Carex. Thus, the phenolic acids' composition cannot be considered as the major taxonomical feature for the genus Carex.

scholarly journals The effect of feeding culture media with biogenetic precursors on high production of depsides in agitated shoot cultures of black and red aronias

2020 ◽  
Vol 142 (2) ◽  
pp. 379-399
Author(s):  
Agnieszka Szopa ◽  
Paweł Kubica ◽  
Łukasz Komsta ◽  
Aleksandra Walkowicz-Bożek ◽  
Halina Ekiert
Keyword(s):  
Caffeic Acid ◽  
Phenolic Acids ◽  
Cinnamic Acid ◽  
Culture Media ◽  
Hplc Method ◽  
Shoot Cultures ◽  
Growth Cycles ◽  
Methanolic Extracts ◽  
Aronia Melanocarpa ◽  
High Production

Abstract Agitated shoot cultures of two aronias, Aronia melanocarpa (Michx.) Elliott and Aronia arbutifolia (L.) Pers., were maintained on Murashige & Skoog medium (1 mg/l BA and 1 mg/l NAA), both with and without the addition of various biosynthetic precursors of phenolic acids and depsides (phenylalanine, cinnamic acid, benzoic acid and caffeic acid). Each substance was added in 5 concentrations (0.1–10 mmol/l), each concentration at two time points (at the beginning and on the 10th day of cultures). Twenty-four phenolic acids were determined in methanolic extracts of the biomasses collected after 20 days of growth cycles by means of HPLC method with DAD detection. The presence of seven compounds was confirmed in all the extracts—five depsides (neochlorogenic, chlorogenic, cryptochlorogenic, isochlorogenic and rosmarinic acids), and syringic and caffeic acids. The main metabolites in A. melanocarpa shoot extracts were isochlorogenic, chlorogenic and neochlorogenic acids (max. 249.88, 450.35, 192.16 mg/100 g DW). The main metabolites in A. arbutifolia shoot extracts were: chlorogenic, isochlorogenic and cryptochlorogenic acids (max. 361.60, 224.5, 526.2 mg/100 g DW). The largest total amounts of the compounds were confirmed in the cultures of both aronias after the addition of cinnamic acid (989.79 and 661.77 mg/100 g DW, respectively) and caffeic acid (854.99 and 1098.46 mg/100 g DW, respectively) at concentrations of 5 mmol/l on 10th day of growth cycles. These maximum amounts were 3.41, 3.42, 2.95 and 5.67 times higher, respectively, than in the control cultures. This is the first report documenting the high production of depsides in shoot cultures of black and red aronias after feeding with their biosynthetic precursors.

scholarly journals Schisandra rubriflora Plant Material and In Vitro Microshoot Cultures as Rich Sources of Natural Phenolic Antioxidants

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Agnieszka Szopa ◽  
Michał Dziurka ◽  
Sebastian Granica ◽  
Marta Klimek-Szczykutowicz ◽  
Paweł Kubica ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
High Performance ◽  
Total Phenolic ◽  
Array Detector ◽  
Plant Materials

Schisandra rubriflora is a dioecious, underestimated medicinal plant species known from traditional Chinese medicine. The present study was aimed at characterising the polyphenolic profile composition and the related antioxidant capacity of S. rubriflora fruit, stem and leaf and in vitro microshoot culture extracts. Separate analyses of material from female and male specimens were carried out. This study was specifically aimed at detailed characterisation of the contribution of phenolic compounds to overall antioxidant activity using ultra-high-performance liquid chromatography with a photodiode array detector coupled to electrospray ionization ion trap mass spectrometry (UHPLC-DAD-ESI-MS3) and a high-performance liquid chromatography-diode array detector (HPLC-DAD). Using UHPLC-DAD-ESI-MS3, twenty-seven phenolic compounds from among phenolic acids and flavonoids were identified. Concentrations of three phenolic acids (neochlorogenic, chlorogenic and cryptochlorogenic acids) and eight flavonoids (hyperoside, rutoside, isoquercitrin, guaijaverin, trifolin, quercetin, kaempferol, and isorhamnetin) were determined using HPLC-DAD using reference standards. The highest total phenolic content was confirmed for the stem and leaf extracts collected in spring. The contents of phenolic compounds of in vitro biomasses were comparable to that in the fruit extracts. The methanolic extracts from the studied plant materials were evaluated for their antioxidant properties using various in vitro assays, namely free radicals scavenging estimation using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), ferric-reducing antioxidant power (FRAP) and cupric-reducing antioxidant capacity (CUPRAC) as well as QUick, Easy, New, CHEap, and Reproducible CUPRAC (QUENCHER-CUPRAC) assays. A close relationship between the content of polyphenolic compounds in S. rubriflora and their antioxidant potential has been documented.

scholarly journals Identification and Determination of Seven Phenolic Acids in Brazilian Green Propolis by UPLC-ESI-QTOF-MS and HPLC

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1791 ◽  
Author(s):  
Shengwei Sun ◽  
Meijuan Liu ◽  
Jian He ◽  
Kunping Li ◽  
Xuguang Zhang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Hplc Method ◽  
Operating Conditions ◽  
Total Phenolic ◽  
Artepillin C ◽  
Brazilian Green Propolis

Brazilian green propolis is a complex mixture of natural compounds that is difficult to analyze and standardize; as a result, controlling its quality is challenging. In this study, we used the positive and negative modes of ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time of flight mass spectrometry in conjunction with high-performance liquid chromatography for the identification and characterization of seven phenolic acid compounds in Brazilian green propolis. The optimal operating conditions for the electrospray ionization source were capillary voltage of 3500 V and drying and sheath gas temperatures of 320 °C and 350 °C, respectively. Drying and sheath gas flows were set to 8 L/min and 11 L/min, respectively. Brazilian green propolis was separated using the HPLC method, with chromatograms for samples and standards measured at 310 nm. UPLC-ESI-QTOF-MS was used to identify the following phenolic compounds: Chlorogenic acid, caffeic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, caffeic acid phenethyl ester (CAPE), and artepillin C. Using a methodologically validated HPLC method, the seven identified phenolic acids were then quantified among different Brazilian green propolis. Results indicated that there were no significant differences in the content of a given phenolic acid across different Brazilian green propolis samples, owing to the same plant resin sources for each sample. Isochlorogenic acid B had the lowest content (0.08 ± 0.04) across all tested Brazilian green propolis samples, while the artepillin C levels were the highest (2.48 ± 0.94). The total phenolic acid content across Brazilian green propolis samples ranged from 2.14–9.32%. Notably, artepillin C quantification is an important factor in determining the quality index of Brazilian green propolis; importantly, it has potential as a chemical marker for the development of better quality control methods for Brazilian green propolis.

scholarly journals Phenolic compounds and properties of antioxidants in grapevine roots (Vitis vinifera L.) under drought stress followed by recovery

2011 ◽  
Vol 78 (2) ◽  
pp. 97-103 ◽  
Author(s):  
Stanisław Weidner ◽  
Monika Karolak ◽  
Magdalena Karamać ◽  
Ryszard Amarowicz
Keyword(s):  
Soil Moisture ◽  
Drought Stress ◽  
Phenolic Compounds ◽  
Vitis Vinifera ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Stress Conditions ◽  
Total Phenolic ◽  
Optimum Conditions ◽  
Fresh Matter

Grapevine seedlings<em> Vitis vinifera</em> were grown in a greenhouse under optimum conditions (soil moisture ca 70%) and under drought stress (soil moisture ca 35%). In addition, some of the plants subjected to drought underwent subsequent regeneration under optimum conditions. Drought stress caused accumulation of total phenolic compounds in grapevine roots, which may indicate that these compounds play an important role in the adaptation of roots to growth under stress conditions. Phenolic acids found in the roots occurred in the ester-bound form only. p-coumaric acid was present in the highest concentrations (6.2 to 10.5 µg/g fresh matter). The content of ferulic acid was lower, ranging from 2.4 to 4.6 µg/g fresh matter. The lowest concentration in grapevine roots was achieved by caffeic acid (2.4 to 2.9 µg/g fresh matter). The levels of p-coumaric and ferulic acids in roots rose significantly under the drought stress, while the concentration of caffeic acid increased during the post-drought recovery period. This may suggest that some of the phenolic acids protect plants under stress conditions. All the extracts from grapevine roots had antioxidative properties, but the antiradical activity of the extracts obtained from roots subjected to drought stress was inferior to the control. The same extracts were also characterised by depressed reducing power. The results imply that tolerance of grapevine to soil drought may be associated with the value of antioxidative potential in root tissues of these plants.

Validation of a RP-HPLC Method for Quantitation of Phenolic Compounds in three Different Extracts from Cymbopogon citratus

2015 ◽  
Vol 9 (7) ◽  
pp. 331-339 ◽  
Author(s):  
Maria Teresa Batista ◽  
Gustavo Costa ◽  
Fatima Nunes ◽  
Carla Vitorino ◽  
Joao Jose Sousa ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Hplc Method ◽  
Cymbopogon Citratus ◽  
Rp Hplc

scholarly journals Comparative study of phenolic acids from underground parts of Rheum palmatum L., R. rhaponticum L. and R. undulatum L.

2011 ◽  
Vol 74 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Helena Danuta Smolarz ◽  
Ewa Medyńska
Keyword(s):  
Comparative Study ◽  
Ferulic Acid ◽  
Phenolic Acids ◽  
Hplc Method ◽  
Rheum Palmatum ◽  
Rp Hplc ◽  
Rheum Undulatum

In three species of <em>Rheum</em> L. genus growing in Poland the composition of phenolic acids was determined. By 2D-TLC method the following acids were identified: ellagic, chlorogenic, gallic, protocatechuic, homoprotocatechuic, caffeic, α-resorcilic, p-hydroxyphenylacetic, p-hydroxybenzoic, p-coumaric, syringic, vanillic and ferulic. There are no substantial qualitative differences among the complex of phenolic acids in the investigated species. The RP-HPLC method was used for quanitative determination of phenolic acids. The amount of individual phenolic acids ranged between 2.2 µg/g and 147.8 µg/g in air-dry rhizome. The content of ferulic acid is the highest in all the examined cases. The total amount of the tested phenolic acids in <em>Rheum undulatum</em> L., <em>R. palmatum</em> L., and <em>R. raponticum</em> L. was respectively 346.4 µg/g, 229.8 µg/g, and 195 µg/g.

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