scholarly journals Acclimation of poplar trees to heavy metals in polluted habitats: l. Carbohydrate metabolism in fine roots of Populus deltoides

2011 ◽  
Vol 74 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Gabriela Lorenc-Plucińska ◽  
Krzysztof Stobrawa
Keyword(s):  
Fine Roots ◽  
Populus Deltoides ◽  
Soluble Sugars ◽  
Chemical Properties ◽  
Heavy Metal Stress ◽  
Control Site ◽  
Pentose Phosphate ◽  
Polluted Site ◽  
Soluble Carbohydrates ◽  
Specific Activities

Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of <em>Populus deltoides</em> Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.

scholarly journals Sucrose degradation in sink and source poplar leaves treated with sulfite

2014 ◽  
Vol 70 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Gabriela Lorenc-Plucińska ◽  
Anna Szadel ◽  
Andrzej Pluciński ◽  
Stanisława Pukacka
Keyword(s):  
Sucrose Synthase ◽  
Populus Deltoides ◽  
Specific Activity ◽  
Soluble Sugars ◽  
Acid Invertase ◽  
Transpiration Stream ◽  
Neutral Invertase ◽  
Specific Activities ◽  
Poplar Leaves

Sulfite at concentrations from 0.5 to 5.0 mM was supplied to illuminated, detached poplar (<em>Populus deltoides</em> Bartr. ex Marsh) leaves via the transpiration stream. Concentrations of chlorophyll <em>a</em> and <em>b</em>, carotenoids, glucose, fructose, sucrose and starch, and extractable specific activities of sucrose synthase (SuSy), acid invertase (AI) and neutral invertase (NI) were measured. In sink and source leaves chlorophylls and carotenoids appeared to be increased by sulfite at concentrations ranging from 0.5 to 5.0 mM. In source leaves the application of 5.0 mM and ≥ 2.5 mM sulfite led to a decrease in total nonstructural soluble sugars (sTNC) and starch, respectively. The specific activity of SuSy was enhanced by ≥ 0.5 mM sulfite. There was only a slight decline in the activity of Al and N1.In sink leaves sTNC decreased and starch increased under the influence of sulfite at concentrations ≤ 1.O mM and ≥ 0.5 mM, respectively. The effect of sulfite on the activity of At, NI and SuSy was negligible. However, in both development groups of leaves alterations in sucrose, glucose and fructose contents were observed. On the basis cf the obtained results we postulate that sulfite changes the sucrolytic activities primarily in source leaves and only marginally in sink leaves.

scholarly journals Carbon and nutrient physiology in shrubs at the upper limits: a multi-species study

2020 ◽  
Author(s):  
Xue Wang ◽  
Fei-Hai Yu ◽  
Yong Jiang ◽  
Mai-He Li
Keyword(s):  
Fine Roots ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Growing Season ◽  
Interactive Effects ◽  
Tree Line ◽  
Shrub Species ◽  
Nutrient Physiology ◽  
Upper Limits ◽  
Species Study

Abstract Aims Carbon and nutrient physiology of trees at their upper limits have been extensively studied, but those of shrubs at their upper limits have received much less attention. The aim of this study is to examine the general patterns of non-structural carbohydrates (NSCs), nitrogen (N) and phosphorous (P) in shrubs at the upper limits, and to assess whether such patterns are similar to those in trees at the upper limits. Methods Across Eurasia, we measured the concentrations of soluble sugars, starch, total NSCs, N and P in leaves, branches and fine roots (&lt; 0.5 cm in diameter) of five shrub species growing at both the upper limits and lower elevations in both summer (peak growing season) and winter (dormancy season). Important Findings Neither elevation nor season had significant effects on tissue N and P concentrations, except for lower P concentrations in fine roots in winter than in summer. Total NSCs and soluble sugars in branches were significantly higher in winter than in summer. There were significant interactive effects between elevation and season for total NSCs, starch, soluble sugars and the ratio of soluble sugar to starch in fine roots, showing lower soluble sugars and starch in fine roots at the upper limits than at the lower elevations in winter but not in summer. These results suggest that the carbon physiology of roots in winter may play an important role in determining the upward distribution of shrubs, like that in the alpine tree-line trees.

scholarly journals STUDY AND ESTIMATION OF THE RATIO OF 137CS AND 40K SPECIFIC ACTIVITIES IN SANDY AND LOAM SOILS / 137CS IR 40K SAVITŲJŲ AKTYVUMŲ SANTYKIŲ TYRIMAS IR VERTINIMAS PRIESMĖLIO IR PRIEMOLIO DIRVOŽEMYJE

2011 ◽  
Vol 3 (5) ◽  
pp. 37-42
Author(s):  
Renata Mikalauskienė ◽  
Donatas Butkus ◽  
Ingrida Pliopaitė Bataitienė
Keyword(s):  
Chemical Properties ◽  
Dry Weight ◽  
Soil Samples ◽  
Physical And Chemical Properties ◽  
Nuclear Testing ◽  
Specific Activities ◽  
Physical And Chemical ◽  
Chernobyl Nuclear Plant ◽  
Properties Of Soil ◽  
And Chemical Properties

The present article describes changes in specific activities and fluctuations in the ratio of natural 40K and artificial 137Cs radionuclides in soil samples taken from different places of Lithuanian territory. The samples of soil have been selected from the districts polluted after the accident in Chernobyl nuclear plant performing nuclear testing operations. The study has established the main physical and chemical properties of soil samples and their impact on the concentration of 40K activities. 137Cs/40K specific activities in soil have been observed under the dry weight of the sample that varied from 0.0034 to 0.0240. The results of the study could be used for establishing and estimating 137Csand 40K transfer in the system “soil-plant”. Santrauka Straipsnyje nagrinėjama gamtinės (40K) ir dirbtinės (137Cs) kilmės radionuklidų savitųjų aktyvumų ir jų santykio kaita skirtinguose Lietuvos teritorijos dirvožemiuose. Dirvožemio mėginiai parinkti iš vietovių, kurios buvo labiau užterštos po Černobylio atominės elektrinės avarijos ir buvusių branduolinių bandymų. Tyrimo metu nustatytos pagrindinės fizinės cheminės dirvožemio savybės ir jų poveikis 40K aktyvumų koncentracijai. 137Csir 40K savitieji aktyvumai dirvožemyje tirti esant sausam mėginio svoriui. 137Cs savitieji aktyvumai sausame dirvožemyje svyravo nuo 1,1±1,0 iki 14,3±0,9 Bq/kg, o 40K savitieji aktyvumai – nuo 326±29 iki 740±15 Bq/kg. 137Csir 40K savitųjų aktyvumų santykis skirtingų vietovių dirvožemiuose kito nuo 0,0034 iki 0,0240 Bq/kg. Tyrimo rezultatai gali būti panaudoti, nustatant ir įvertinant 137Csir 40K pernašą sistemoje dirvožemis–augalai.

THE PHYSIOLOGY OF HOST-PARASITE RELATIONS: IX. FURTHER OBSERVATIONS ON THE ACCUMULATION OF RADIOACTIVE SUBSTANCES AT RUST INFECTIONS

1961 ◽  
Vol 39 (6) ◽  
pp. 1393-1407 ◽  
Author(s):  
Michael Shaw
Keyword(s):  
Specific Activity ◽  
Dry Weight ◽  
Insoluble Fraction ◽  
Pentose Phosphate ◽  
Accumulation Ratio ◽  
X Ray ◽  
Specific Activities ◽  
Leaf Segments ◽  
Wheat Leaves ◽  
Host Parasite

Wang (Can. J. Botany, 38, 635–642 (1960)) concluded that the accumulation of radioactivity observed on radioautographs at infection sites on rusted leaves fed with C14-labelled substances was 'apparent' rather than real. The ‘accumulation ratio’ is defined as the ratio of the specific activities (c.p.m./mg dry weight of intact tissue) of rust-infected to uninfected areas of infected leaves. Theoretical considerations relating to the radioautography of leaves labelled with C14 and to the measurement of ‘accumulation ratios’ by extraction of C14-labelled substances from rusted and uninfected segments of infected leaves, as well as experimental data, show that Wang's conclusion is not generally applicable.Experimentally, it was shown using polymethacrylate C14 sources that differences in distance between sources and X-ray film of the order of 100 μ had no effect on the intensity of autoradiographs. Rust-infected leaves, fed with radioactive glucose, were radiographed between X-ray plates. Localization of radioactivity at infection sites was observed on both ‘dorsal’ and ‘ventral’ radiographs, indicating a real accumulation per unit area. Ventral were more radioactive than dorsal surfaces. The main development of the fungus occurred on the former. Radioautography revealed that C14 from glucose-1-C14, glucose-6-C14, and uniformly labelled glucose fed to excised wheat leaves became localized at 10-day-old rust infections in 2 hours. ‘Accumulation ratios’ calculated from the specific activity of leaf segments remained close to 1.0 for at least 6 hours after introduction of the tracer, but increased to more than 2 after 24 hours. When ‘accumulation ratios’ were calculated from the specific activities of individual pustules (excised with a punch 1 mm in diameter) and interpustular disks, values greater than 1 were observed in 2 hours, thus confirming the results of autoradiography. Differences between the ‘accumulation ratios’ observed with glucose-6-C14 and glucose-1-C14 were consistent with an increased role of the pentose phosphate pathway at infection sites. Incorporation of C14 from uniformly labelled glucose into the alcohol-insoluble fraction of rusted leaf segments was 2.5-fold that in uninfected segments in 6 hours and 3.65-fold in 24 hours. The humin formed during hydrochloric acid hydrolysis accounted for approximately 50% of the activity of the alcohol-insoluble material. The ‘accumulation ratio’ for the alcohol-soluble material was only 1.56 after 24 hours.All the results support the view (Shaw and Samborski, Can. J. Botany, 34, 389–405 (1956)) that there is a quantitative, metabolically dependent accumulation of C14 from radioactive glucose at vigorous rust infections. The relative roles of fungus and host in this process are discussed briefly.

Fine root biomass and nutrient content in a black spruce peat soil with and without alder

1998 ◽  
Vol 78 (1) ◽  
pp. 163-169 ◽  
Author(s):  
J. S. Bhatti ◽  
N. W. Foster ◽  
P. W. Hazlett
Keyword(s):  
Fine Roots ◽  
Root Biomass ◽  
Fine Root ◽  
Black Spruce ◽  
Peat Soil ◽  
Chemical Properties ◽  
Nutrient Content ◽  
Fine Root Biomass ◽  
Boreal Peatlands ◽  
Strong Positive Relationship

Vertical distribution of fine root biomass and nutrient content was examined within a black spruce (Picea mariana) stand growing on a boreal peat soil in northeastern Ontario. The influence of site physical and chemical properties on fine root biomass production was assessed. More then 80% of the fine roots were present in moss plus the top 10 cm of peat where nutrients and aeration are most favourable. The fine root biomass (W/V) was significantly higher with alder (5.9 kg m−3) (Alnus rugosa) as understory vegetation compared to non-alder locations (2.9 kg m−3). Total nutrient content in fine roots was 54, 3.2, 5.4, 63 and 5.7 kg ha−1 on the alder site and 20, 1.4, 2.3, 28 and 4.2 kg ha−1 of N, P, K, Ca, and Mg on the non-alder site, respectively. The mass (W/V) of nutrients in fine roots was strongly dependent upon the availability of nutrients in the peat. Fine root content had a strong positive relationship with peat available P and exchangeable K contents suggesting that P and K may be limiting nutrients for black spruce in this peat soil. Key words: Nitrogen, phosphorus, potassium, boreal peatlands, aeration, water table

scholarly journals Soluble carbohydrates in cereal (wheat, rye, triticale) seed after storage under accelerated ageing conditions

2011 ◽  
Vol 79 (1) ◽  
pp. 21-25
Author(s):  
Agnieszka I. Piotrowicz-Cieślak ◽  
Maciej Niedzielski ◽  
Dariusz J. Michalczyk ◽  
Wiesław Łuczak ◽  
Barbara Adomas
Keyword(s):  
Moisture Content ◽  
Spring Wheat ◽  
Soluble Sugars ◽  
Dry Mass ◽  
Soluble Carbohydrates ◽  
Accelerated Ageing ◽  
Winter Rye ◽  
Seed Moisture Content ◽  
Winter Triticale ◽  
Seed Moisture

Germinability and the content of soluble carbohydrates were analysed in cereal seed (winter rye, cv. Warko; spring wheat, cv. Santa; hexaploid winter triticale, cv. Fidelio and cv. Woltario). Seed moisture content (mc) was equilibrated over silica gel to 0.08 g H<sub>2</sub>O/g dry mass and stored in a desiccator at 20<sup>o</sup>C for up to 205 weeks or were equilibrated to mc 0.06, 0.08 or 0.10 g H<sub>2</sub>O/g dm and subjected to artificial aging at 35<sup>o</sup>C in air-tight laminated aluminium foil packages for 205 weeks. It was shown that the rate of seed aging depended on the species and seed moisture content. The fastest decrease of germinability upon storage was observed in seed with the highest moisture level. Complete germinability loss for winter rye, winter triticale cv. Fidelio, winter triticale cv. Woltario and spring wheat seed with mc 0.10 g H<sub>2</sub>O/g dm<sup>3</sup> occurred after 81, 81, 101 and 133 weeks, respectively. Fructose, glucose, galactose, myo-inositol, sucrose, galactinol, raffinose, stachyose and verbascose were the main soluble carbohydrates found in the seed. The obtained data on the contents of specific sugars and the composition of soluble sugars fraction in seed of rye, wheat and triticale did not corroborate any profound effect of reducing sugars, sucrose and oligosaccharides on seed longevity.

scholarly journals Preliminary Study on Effect of Early Defoliation on Dry Matter Accumulation and Storage of Reserves on ‘Abbé Fétel’ Pear Trees

HortScience ◽  
2019 ◽  
Vol 54 (12) ◽  
pp. 2169-2177 ◽  
Author(s):  
Karen Mesa ◽  
Sara Serra ◽  
Andrea Masia ◽  
Federico Gagliardi ◽  
Daniele Bucci ◽  
...  
Keyword(s):  
Dry Matter ◽  
Management Practices ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Growing Season ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
Sink Strength ◽  
Dry Matter Accumulation ◽  
Pear Trees

Annual accumulation of starch is affected by carbon reserves stored in the organs during the growing season and is controlled mainly by sink strength gradients within the tree. However, unfavorable environmental conditions (e.g., hail events) or application of management practices (e.g., defoliation to enhance overcolor in bicolor apple) could influence the allocation of storage carbohydrates. This preliminary research was conducted to determine the effects of early defoliation on the dry matter, starch, and soluble carbohydrate dynamics in woody organs, roots, and mixed buds classified by age and two levels of crop-load for one growing season in ‘Abbé Fétel’ pear trees (Oct. 2012 to mid-Jan. 2013 in the northern hemisphere). Regardless of the organs evaluated (woody organs, roots, and mixed buds), an increase of soluble carbohydrate concentration was observed in these organs in the period between after harvest (October) and January (dormancy period). Among all organs, woody short-old spurs showed the highest increase (+93.5%) in soluble sugars. With respect to starch, woody organs showed a clear trend of decreasing in concentration between October and January. In this case, short-old spurs showed the smallest decline in starch concentrations, only 6.5%, whereas in other tree organs starch decreased by 34.5%. After harvest (October), leaves showed substantially higher starch and soluble sugar concentrations in trees with lower crop-loads. These results confirm that in the period between October and January, dynamic interconversions between starch and soluble carbohydrates occur at varying magnitudes among organs in pear trees.

scholarly journals Does the Mixture of Species change the C:N:P Ecological Stoichiometric Characteristics of fine Roots? A Case Study of Cupressus Funebris Mixed Forest

2021 ◽  
Author(s):  
Wenchun He ◽  
Yang Wang ◽  
Xiaochen Wen ◽  
Yu Wang ◽  
Baoru Xiao ◽  
...  
Keyword(s):  
Fine Roots ◽  
Environmental Changes ◽  
Mixed Forest ◽  
Chemical Properties ◽  
Available Phosphorus ◽  
Biological Factors ◽  
Nitrogen And Phosphorus ◽  
Mixed Stands ◽  
Available Nitrogen ◽  
Low Efficiency

Abstract The growth of fine roots of trees is affected by environmental changes and biological factors. At present, there have been many researches on the physiological plasticity of fine roots caused by environmental changes, but there are still few studies on the influence of biological factors on fine roots. This paper focused on the contents of carbon (C), nitrogen (N), and phosphorus (P), and their ecological stoichiometric ratios in different root orders of Cupressus funebris fine roots in 11 mixed stands with Koelreuteria paniculate or Toona sinensis at different ratios, and the effects of soil physical and chemical properties on the root chemical properties. It aimed to provide new insights into the fine-root nutrient distribution pattern and the transformation or reconstruction of low-efficiency pure forests from the standpoint of forest types. The results showed that: soil pH, and the content of available nitrogen (SAN), available phosphorus (SAP) and available potassium (SAK) differed significantly in the tested mixed forest stands. No significant differences in carbon content of fine roots were observed in different mixed stands. The content of nitrogen and phosphorus in fine roots in mixed forests showed heterogeneity. Species mixing changed the C/N, C/P and N/P of the C. funebris compared the pure stands. The "T. sinensis + C. funebris" forest alleviated the limitation of the lack of phosphorus on fine roots of C. funebris on. The principal component analysis showed that mixed stands of "T. sinensis + C. funebris" had the highest comprehensive score at ratio of "3:1". Thus, our results recommended the adoption of T. sinensis, especially at 75%, to reconstruct the low-efficiency pure C. funebris forest.

scholarly journals PROPRIEDADES QUÍMICAS E CÔMPUTO QUÍMICO DOS AMINOÁCIDOS DA FARINHA E CONCENTRADO PROTÉICO DE FEIJÃO GUANDU (Cajanus cajan (L.) Millsp)

2000 ◽  
Vol 18 (2) ◽  
Author(s):  
IVONE YURIKA MIZUBUTI ◽  
LUIZ WALDEMAR DE OLIVEIRA SOUZA ◽  
OSWALDO BIONDO JÚNIOR ◽  
ELZA IOUKO IDA
Keyword(s):  
Amino Acids ◽  
Chemical Composition ◽  
Cajanus Cajan ◽  
Soluble Sugars ◽  
Extraction Procedure ◽  
Chemical Properties ◽  
Pigeon Pea ◽  
Protein Concentrate ◽  
Chemical Score ◽  
Sulphur Amino Acids

Esta pesquisa teve por objetivo investigar as propriedades químicas e cômputo químico dos aminoácidos da farinha e de concentrado protéico de feijão guandu (Cajanus cajan (L.) Millsp). A farinha apresentou composição química adequada em relação a outras leguminosas. O procedimento de extração aquosa de proteína, a partir de grãos de feijão guandu com 25,73% de proteína, permitiu a obtenção de concentrado protéico com 52,47% de proteína, em base seca, compreendendo concentração da ordem de 103,85%. O concentrado protéico de feijão guandu apresentou composição química adequada, sendo que cinzas e açúcares solúveis foram concentrados 122,17 e 21,86%, respectivamente, em relação ao grão. Os níveis de aminoácidos no concentrado protéico foram similares aos de farinha de guandu, porém, a metionina foi concentrada em 42,64%. O cômputo químico indicou que os aminoácidos limitantes primários na farinha e concentrado protéico foram os sulfurados totais. A composição química da farinha e do concentrado protéico de guandu indicou potencialidade para utilização de ambos como componentes de alimentos para seres humanos e animais. Abstract The aim of this work was the investigation of the chemical properties of Pigeon pea (Cajanus cajan) (L.) Millsp) flour and protein concentrate. The flour presented suitable chemical composition in comparison to other legumes. The water extraction procedure of protein, from Pigeon pea grains with 25,73% of protein allowed to obtain a protein concentrate with 52,47% of protein in dry basis, resulting an increase in order of 103,85%. The protein concentrate of Pigeon pea showed suitable chemical composition, having an increase of 122,17% in ashes and 21,86 % in soluble sugars in relation to the crude grain. The amino acids levels in the concentrate were similar to those in the flour, however there were an increase of 42,64% in methionine concentration. The chemical score suggest that the primary limiting amino acids in the flour and in the concentrate were the total sulphur amino acids. The chemical composition of the flour and concentrate of Pigeon pea showed perspectives of its utilization as feed components for humans and animals.

Metabolism of glucose-14C, pyruvate-14C, and mannitol-14C by Melampsora lini. II. Conversion to soluble products

1968 ◽  
Vol 46 (4) ◽  
pp. 453-460 ◽  
Author(s):  
D. Mitchell ◽  
Michael Shaw
Keyword(s):  
Pentose Phosphate Pathway ◽  
Tricarboxylic Acid Cycle ◽  
Rust Fungus ◽  
Tca Cycle ◽  
Tricarboxylic Acid ◽  
Pentose Phosphate ◽  
Soluble Carbohydrates ◽  
Melampsora Lini ◽  
Carbohydrate Fraction ◽  
The Tca Cycle

Mycelium of the flax rust fungus (Melampsora lini (Pers.) Lév.), grown on flax cotyledons in tissue culture, had a mean [Formula: see text]of 4.1 and a mean C6/C1 ratio of 0.14, measured after 4 hours in radioactive glucose. The C6/C1 ratio increased with time and also after treatment with 10−5 M 2,4-dinitrophenol. The relative labelling of the (80%) ethanol-soluble carbohydrates, and organic and amino acid fractions after incubation with glucose-1-, -2-, or -6-14C also indicated preferential release of C1 as 14CO2. Trehalose (unknown A) was tentatively identified in the carbohydrate fraction and was mildly radioactive after incubation of the mycelium with labelled glucose for 3 hours. The principal radioactive products of glucose in this fraction were two unknowns, B and C, which were tentatively identified as mannitol and arabitol. The labelling patterns were consistent with their formation from intermediates of the pentose phosphate pathway. The distribution of radioactivity derived from glucose in alanine, glutamate, and aspartate also indicated that hexose or triose units formed in the pentose phosphate pathway were converted to pyruvate, which either gave rise to alanine or was further oxidized in the tricarboxylic acid cycle. Incubation with pyruvate-1-, -2-, or -3-14C for 3 hours gave rise to 14CO2 and labelled alanine, glutamate, and aspartate in a manner consistent with the operation of the TCA cycle. Mannitol-1-6-14C was not metabolized to any appreciable extent in this period, but did give rise to 14CO2 and to several unidentified compounds in the carbohydrate fraction.

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