scholarly journals Water deficit-induced membrane injury and oxidative stress in two barley genotypes

2014 ◽  
Vol 71 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Hanna Bandurska ◽  
Jolanta Floryszak-Wieczorek
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Water Stress ◽  
Water Deficit ◽  
Superoxide Radical ◽  
Membrane Injury ◽  
Induced Membrane ◽  
Radical Generation ◽  
And Oxidative Stress ◽  
Barley Genotypes

The purpose of this paper was to examine the effect of water deficit on membrane injuries, superoxide radical generation and lipid peroxidation in the leaves of two barley genotypes. Six-day-old seedlings of the cv. Aramir and line R567 were used in the experiments. According to our earlier work these genotypes significantly differ in the level of membrane injuries under water deficit conditions. Water stress was applied directly to leaves or to roots. The stress caused considerable membrane injuries in the leaves of all genotypes investigated.The percentage membrane injury was higher in the line R567 than in the cv. Aramir. Water stress imposed on leaves caused higher membrane injuries than water stress imposed on roots. The water stress treatment followed by an oxidative stress in the leaves. Line R567 having noticeably larger membrane injuries also exhibited a higher level of superoxide radical generation than the cv. Aramir. The level of lipid peroxidation increased in the both genotypes under the conditions of water stress imposed on leaves, but not on roots.

Essential nutrients and oxidative stress of durum wheat under water stress.

2014 ◽  
Vol 3 (1) ◽  
pp. 123-128
Author(s):  
Issaad Ghozlène ◽  
Djebar Mohammed-Réda ◽  
Berrebbah Houria ◽  
Charga Ali
Keyword(s):  
Oxidative Stress ◽  
Water Stress ◽  
Durum Wheat ◽  
Water Deficit ◽  
Triticum Durum ◽  
Metabolic Disturbances ◽  
Essential Nutrients ◽  
Plant Model ◽  
The Subject ◽  
And Oxidative Stress

Oxidative stress in plants is the subject of numerous reviews of the literature (Apel and Hirt, 2004; Noctor and Foyer, 2005a; Pitzschke et al, 2006;. Wormuth et al, 2007;. Sbartai et al, 2012.) And of several books (Inze and Montagu, 2001; Smirnoff et al, 2005.). This work focuses on studying the effects of water stress on the roots of a plant model: durum wheat (Triticum durum) variety GTA. After the germination, the plants were subjected to water stress during (03, 05, 07 and 09 days). Our results demonstrate abiochemical and metabolic disturbances of the seed subjected to water deficit. The lack of water has caused significant changes in the GTA durum variety.

Nose-only water-pipe smoking effects on airway resistance, inflammation, and oxidative stress in mice

2013 ◽  
Vol 115 (9) ◽  
pp. 1316-1323 ◽  
Author(s):  
Abderrahim Nemmar ◽  
Haider Raza ◽  
Priya Yuvaraju ◽  
Sumaya Beegam ◽  
Annie John ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Airway Resistance ◽  
Reduced Glutathione ◽  
Mechanistic Explanation ◽  
The United States ◽  
Forced Oscillation Technique ◽  
Water Pipe ◽  
Respiratory Effects ◽  
And Oxidative Stress

Water-pipe smoking (WPS) is a common practice in the Middle East and is now gaining popularity in Europe and the United States. However, there is a limited number of studies on the respiratory effects of WPS. More specifically, the underlying pulmonary pathophysiological mechanisms related to WPS exposure are not understood. Presently, we assessed the respiratory effects of nose-only exposure to mainstream WPS generated by commercially available honey flavored “moasel ” tobacco. The duration of the session was 30 min/day and 5 days/wk for 1 mo. Control mice were exposed to air only. Here, we measured in BALB/c mice the airway resistance using forced-oscillation technique. Lung inflammation was assessed histopathologically and by biochemical analysis of bronchoalveolar lavage (BAL) fluid, and oxidative stress was evaluated biochemically by measuring lipid peroxidation, reduced glutathione and several antioxidant enzymes. Pulmonary inflammation assessment showed an increase in neutrophil and lymphocyte numbers. Likewise, airway resistance was significantly increased in the WPS group compared with controls. Tumor necrosis factor α and interleukin 6 concentrations were significantly increased in BAL fluid. Lipid peroxidation in lung tissue was significantly increased whereas the level and activity of antioxidants including reduced glutathione, glutathione S transferase, and superoxide dismutase were all significantly decreased following WPS exposure, indicating the occurrence of oxidative stress. Moreover, carboxyhemoglobin levels were significantly increased in the WPS group. We conclude that 1-mo nose-only exposure to WPS significantly increased airway resistance, inflammation, and oxidative stress. Our results provide a mechanistic explanation for the limited clinical studies that reported the detrimental respiratory effects of WPS.

scholarly journals The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

2014 ◽  
Vol 66 (3) ◽  
pp. 1075-1081
Author(s):  
Ivan Simic ◽  
Violeta Iric-Cupic ◽  
Rada Vucic ◽  
Marina Petrovic ◽  
Violeta Mladenovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Brain ◽  
Wistar Rats ◽  
Superoxide Radical ◽  
Reduced Glutathione ◽  
Oxidative Stress Markers ◽  
Sod Activity ◽  
Stress Markers ◽  
Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

scholarly journals The Current Trends in Using Nanoparticles, Liposomes, and Exosomes for Semen Cryopreservation

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2281
Author(s):  
Islam M. Saadeldin ◽  
Wael A. Khalil ◽  
Mona G. Alharbi ◽  
Seok Hee Lee
Keyword(s):  
Oxidative Stress ◽  
Artificial Insemination ◽  
Pleiotropic Effect ◽  
Bioactive Molecules ◽  
Membrane Injury ◽  
Semen Cryopreservation ◽  
Freeze Thaw ◽  
Current Trends ◽  
And Oxidative Stress ◽  
Different Levels

Cryopreservation is an essential tool to preserve sperm cells for zootechnical management and artificial insemination purposes. Cryopreservation is associated with sperm damage via different levels of plasma membrane injury and oxidative stress. Nanoparticles are often used to defend against free radicals and oxidative stress generated through the entire process of cryopreservation. Recently, artificial or natural nanovesicles including liposomes and exosomes, respectively, have shown regenerative capabilities to repair damaged sperm during the freeze–thaw process. Exosomes possess a potential pleiotropic effect because they contain antioxidants, lipids, and other bioactive molecules regulating and repairing spermatozoa. In this review, we highlight the current strategies of using nanoparticles and nanovesicles (liposomes and exosomes) to combat the cryoinjuries associated with semen cryopreservation.

scholarly journals Effect of High Glucose-Induced Oxidative Stress on Paraoxonase 2 Expression and Activity in Caco-2 Cells

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1616 ◽  
Author(s):  
Camilla Morresi ◽  
Laura Cianfruglia ◽  
Davide Sartini ◽  
Monia Cecati ◽  
Stefania Fumarola ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Free Radical ◽  
High Glucose ◽  
Gastrointestinal Diseases ◽  
Intestinal Cells ◽  
Glycation End Products ◽  
Radical Generation ◽  
Apoptotic Pathways ◽  
Expression And Activity

(1) Background: Hyperglycemia leads to several biochemical and physiological consequences, such as the generation of advanced glycation end products (AGEs) and reactive oxygen species (ROS), which are involved in the development of several human diseases. Intestinal cells are continuously exposed to pro-oxidants and lipid peroxidation products from ingested foods, and also to glyco-oxidative damage. It has been reported that free radical generation may be linked to the development of inflammation-related gastrointestinal diseases. (2) Methods: The effects of high glucose (HG) treatment (50 mM) were assessed in terms of free radical production, lipid peroxidation, and AGEs formation. Furthermore, the expression and the antiapoptotic and antioxidant activity of the paraoxonase-2 (PON2) enzyme in intestinal cells has been investigated. (3) Results: Caco-2 cells treated with media supplied with high glucose (HG) (50 mM) showed, with respect to physiological glucose concentration (25 mM), an increase in ROS production, lipid peroxidation, and AGEs formation. Moreover, a lower PON2 expression and activity in HG-treated cells was related to activation of the apoptotic pathways. (4) Conclusions: Our results demonstrated that high glucose concentrations triggered glyco-oxidative stress in intestinal cells; the downregulation of PON2 could result in a higher oxidative stress and might contribute to intestinal dysfunction.

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