scholarly journals Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 69 (2) ◽  
pp. 115-117 ◽  
Author(s):  
Janusz Malarz ◽  
Wanda Kisiel
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Control Level ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Direct Addition

Direct addition of pectinase as an elicitor to the hairy root culture of <em>Lactuca virosa</em> L. transformed with <em>Agrobacterium rhizogenes</em> stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment.

Sesquiterpene lactones in Agrobacterium rhizogenes—Transformed hairy root culture of Lactuca virosa

1995 ◽  
Vol 40 (4) ◽  
pp. 1139-1140 ◽  
Author(s):  
Wanda Kisiel ◽  
Anna Stojakowska ◽  
Janusz Malarz ◽  
Stanislaw Kohlmüzer
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Transformed Hairy Root

scholarly journals Effect of methyl jasmonate on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 68 (2) ◽  
pp. 119-121 ◽  
Author(s):  
Janusz Malarz ◽  
Wanda Kisiel
Keyword(s):  
Methyl Jasmonate ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hplc Analysis ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Methyl Jasmonate Treatment

Hairy roots of <em>Lactuca virosa</em> L. transformed with <em>Agrobacterium rhizogenes</em> and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control.

scholarly journals Hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 64 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Anna Stojakowska ◽  
Janusz Malarz ◽  
Wanda Kisiel ◽  
Stanisław Kohlmünzer
Keyword(s):  
Growth Rate ◽  
Hairy Root ◽  
Chromatographic Analysis ◽  
Sesquiterpene Lactones ◽  
Leaf Explants ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Nutrient Media ◽  
Lactuca Virosa

A hairy root culture of <i>Lactuca virosa</i> L. (<i>Asteraceae</i>) was initiated by infection of the leaf explants of the aseptic plants with <i>Agrobacterium rhizogenes</i> - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant.

A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum

Biologia ◽  
2014 ◽  
Vol 69 (7) ◽  
Author(s):  
Elnaz Nourozi ◽  
Bahman Hosseini ◽  
Abbas Hassani
Keyword(s):  
Salicylic Acid ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
High Performance ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Biochemical Properties ◽  
Separate Experiment ◽  
Transformed Roots

AbstractHairy root culture system is a valuable tool to study the characteristics of gene expression, gene function, root biology, biochemical properties and biosynthesis pathways of secondary metabolites. In the present study, hairy roots were established in Anise hyssop (Agastache foeniculum) via Agrobacterium rhizogenes. Three strains of Agrobacterium rhizogenes (A4, A7 and 9435), were used for induction of hairy roots in four various explants (hypocotyl, cotyledon, one-month-old leaf and five-month-old leaf) of Anise hyssop. The highest frequency of transformation was achieved using A4 strain in one-month-old leaves (51.1%). The transgenic states of hairy root lines were confirmed by PCR (Polymerase chain reaction) method. High performance liquid chromatography analysis revealed that the production of rosmarinic acid (RA) in transformed roots of A. foeniculum was almost 4-fold higher than that of the non-transformed roots. In a separate experiment, hairy roots obtained from one-month-old leaves inoculated with A4 strain, were grown in liquid medium and the effects of different concentrations of salicylic acid (0.0, 0.01, 0.1 and 1 mM) and chitosan (0, 50, 100 and 150 mg L−1) (as elicitor) and sucrose (20, 30, 40 and 50 g L−1) on the growth of hairy roots were evaluated. The results showed that, 30 g L−1 sucrose and 100 mg L−1 chitosan increased the biomass of hairy root cultures and application of salicylic acid reduced the growth of hairy roots compared with control roots.

Technology and Application of Hairy Root Culture in Monocotyledons

2021 ◽  
Vol 07 ◽  
Author(s):  
Chang-Qi Hao ◽  
Shuai-Run Wang ◽  
Yi Wang ◽  
Xin-Yi Hou ◽  
Ya-Xuan Jiang ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Food Crops ◽  
Root Induction ◽  
Hairy Root Induction ◽  
Selection Of

Background: Hairy root culture has been widely used in the production of metabolites in dicotyledons, and a large number of food crops and medicinal plants in monocotyledons need to be developed, but there are many difficulties in the induction of hairy roots in monocotyledons. The purpose of this paper is to introduce the inducing methods, influencing factors and application of hairy roots in monocotyledons, and to promote the development of hairy root system in monocotyledons. Methods: The mechanism of action of Agrobacterium rhizogenes and the current situation of hairy root induction, induction methods and influencing factors of monocotyledons were summarized so as to provide convenience for efficient acquisition of hairy root of monocotyledons. Results: Monocotyledons are not easy to produce phenols, cells are prone to lignification, adverse differentiation and selective response to Agrobacterium rhizogenes strains. It is proposed that before induction, plant varieties and explants should be selected, and different infection strains should be screened. In the process of hairy root induction, exogenous inducers such as acetosyringone can be added. Although these factors can provide some help for the induction of hairy roots in monocotyledons, we still need to pay attention to the disadvantages of monocotyledons from dicotyledons at the cellular level. Conclusion: A large number of food crops and medicinal plants are monocotyledons. Hairy root culture can be used to help the breeding and production of medicinal substances. Therefore, it is necessary to pay attention to the selection of varieties and explants, the selection of Agrobacterium rhizogenes and the addition of acetosyringone in the process of hairy root induction so as to improve the production efficiency and facilitate the development and utilization of monocotyledons.

Polyprenols in hairy roots of Coluria geoides

2000 ◽  
Vol 28 (6) ◽  
pp. 790-791 ◽  
Author(s):  
K. Skorupińska-Tudek ◽  
V. S. Hung ◽  
O. Olszowska ◽  
M. Furmanowa ◽  
T. Chojnacki ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Lipid Content ◽  
Mammalian Cells ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
Living Organisms ◽  
Long Chain

Long-chain polyisoprenoid alcohols built from several up to more than 100 isoprenoid units are common constituents of all living organisms. They were found mostly in plants, bacteria, yeasts and mammalian cells. In vitro hairy root culture of Coluria geoides was obtained from plants transformed with Agrobacterium rhizogenes. Growth was optimal at 0.75% (w/v) glucose and at 22 °C. Dry samples of roots were extracted and lipid content was analysed by HPLC. According to our estimation, polyprenols are accumulated in roots of C. geoides cultivated in vitro as a mixture of several prenologues with the dominating prenol composed of 16 isoprenoid units. The content of polyprenols in tissue was approx. 300 μg/g of dry weight.

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