scholarly journals Rhodiola rosea in vitro culture - phytochemical analysis and antioxidant action

2014 ◽  
Vol 67 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Mirosława Furmanowa ◽  
Ewa Skopińska-Rozewska ◽  
Ewa Rogala ◽  
Małgorzata Hartwich
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Culture ◽  
Caffeic Acid ◽  
Hplc Method ◽  
Rhodiola Rosea ◽  
Phytochemical Analysis ◽  
Chemiluminescence Method ◽  
Phytochemical Investigation ◽  
Yellow Line

Various <em>Rhodiola rosea</em> organs and tissues from in vitro culture were studied in two areas: searching for the biological active four phenolic compounds and measurement of antioxidant activity of dry residues of EtOH-extracts soluble in PBS using chemiluminescence method. For phytochemical investigation HPLC method was used. Salidroside was typical for organs of intact plant, rosavin for roots of different origin and shoots of intact plants, triandrin was more typical for tissue cultured in vitro, but it was found in all studied samples. Caffeic acid was detected mainly in green and yellow lines of callus, less in other <em>Rhodiola rosea</em> plant material. The highest antioxidant activity showed the extract from 1,5-years-old callus of green and yellow line, which contained caffeic acid and triandrin. Further observations are in progress.

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

scholarly journals Phytochemical Analysis of Anti-Inflammatory and Antioxidant Effects of Mahonia aquifolium Flower and Fruit Extracts

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Andra-Diana Andreicut ◽  
Alina Elena Pârvu ◽  
Augustin Cătălin Mot ◽  
Marcel Pârvu ◽  
Eva Fischer Fodor ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Stress Index ◽  
Tnf Alpha ◽  
Phytochemical Analysis ◽  
Anti Inflammatory ◽  
Mahonia Aquifolium ◽  
Antioxidant Effects

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.

PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL PROPERTIES OF DIOSCOREA BULBIFERA TUBER

2017 ◽  
Vol 10 (12) ◽  
pp. 317
Author(s):  
Dahiya P
Keyword(s):  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Investigation ◽  
Dioscorea Bulbifera ◽  
Acinetobacter Sp ◽  
Almost All

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.

Comparative efficacy of antioxidant drugs in an in vitro experiment

2021 ◽  
Vol 2021 (2) ◽  
pp. 37-40
Author(s):  
Kseniya Smirnova ◽  
Dmitriy Gildikov
Keyword(s):  
Antioxidant Activity ◽  
Test System ◽  
Comparative Efficacy ◽  
Chemiluminescence Method ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Oxidation System

The aim of this work was to study the antioxidant activity of 3-hydroxypyridine derivatives: Mexidol-Vet® and Emycidin in a model oxidation system. Using the chemiluminescence method, it was found that the introduction of Mexidol-Vet® into the test system at a dose of 1 mg reliably suppresses the amplitude of a fast flash and decreases the formation of photons (light sum value), compared with the test system / placebo and the test system / Emicidin. The antioxidant activity of Mexidol-Vet® is significantly higher than Emicidin by 28.1%.

scholarly journals Evaluation of In Vitro Antioxidant and Antidiabetic Activities of Aristolochia longa Extracts

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Nasreddine El Omari ◽  
Karima Sayah ◽  
Saad Fettach ◽  
Omar El Blidi ◽  
Abdelhakim Bouyahya ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Inhibitory Effect ◽  
Phytochemical Analysis ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Therapeutic Tools

Oxidative stress plays a major role in diabetic physiopathology; hence, the interest of using natural antioxidants as therapeutic tools exists. The aim of this study was the evaluation of in vitro antioxidant activity and inhibitory potential of organic extracts from Aristolochia longa roots against key enzymes linked to hyperglycemia. Antioxidant activity was performed using 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals and ferric reducing/antioxidant power (FRAP) methods. The α-Glucosidase and β-Galactosidase inhibitory activities were investigated using an in vitro model. Moreover, phytochemical analysis of tested extracts was carried out. The aqueous fraction of this herb exhibited the highest antioxidant activity for both DPPH and ABTS methods, IC50=125.40±2.40 μg/mL and IC50=65.23±2.49 μg/mL, respectively. However, the ethyl acetate fraction possessed the strongest inhibitory effect towards α-Glucosidase (IC50=1.112±0.026 mg/mL). Furthermore, the result showed high levels of phenolic content. The results showed that this plant could be a significant source of medically important natural compounds.

In vitro methods to determine the antioxidant activity of caffeic acid

2019 ◽  
Vol 219 ◽  
pp. 358-366 ◽  
Author(s):  
Caroline Magnani Spagnol ◽  
Renata Pires Assis ◽  
Iguatemy Lourenço Brunetti ◽  
Vera Lucia Borges Isaac ◽  
Hérida Regina Nunes Salgado ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Caffeic Acid ◽  
In Vitro Methods

In vitro culture of Rhodiola rosea L.

2016 ◽  
Author(s):  
O.N. Khapilina ◽  
◽  
Z.S. Kupeshev ◽  
A.N. Danilova ◽  
R.N. Kalendar
Keyword(s):  
In Vitro Culture ◽  
Rhodiola Rosea

Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir

2012 ◽  
Vol 6 (1) ◽  
pp. 67 ◽  
Author(s):  
Mohd Mujeeb ◽  
Kamran Ashraf ◽  
Mohammad Aqil ◽  
Mohd Amir ◽  
Ahsanullah Khan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity

In vitro antioxidant activity and phytochemical analysis of Teucrium pseudo-Scorodonia Desf. Collected from Algeria

2017 ◽  
Vol 17 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Karima Belarbi ◽  
Fawzia Atik-Bekkara ◽  
Imad Abdelhamid El Haci ◽  
Ilhem Bensaid ◽  
Fawzia Beddou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Analysis ◽  
In Vitro Antioxidant Activity
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