scholarly journals Catharanthus roseus (L.) G. Don - plant regeneration and alkaloids content

2014 ◽  
Vol 63 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Mirosława Furmanowa ◽  
Hanna Olędzka ◽  
Joanna Józefowicz ◽  
Agnieszka Pietrosiuk
Keyword(s):  
Plant Regeneration ◽  
Butyric Acid ◽  
Catharanthus Roseus ◽  
Juvenile Stage ◽  
Shoot Tips ◽  
Axillary Buds ◽  
Indolylacetic Acid

We describe here a regeneration of plantlets of <i>Catharanthus roseus</i> (L.) G. Don from shoot tips and axillary buds. Shoot tips were excised from 7-day-old seedlings and were incubated in solid Nitsch and Nitsch (NN) medium supplemented with kinetin, benzyladenine (BA), indole-3-butyric acid (IBA) and β-indolylacetic acid (IAA) in various combinations. After two months in culture, regenerated rooted plantlets were cut and transferred to a new medium; the explants contained shoot tips or axillary buds. Four passages were done. We obtained about 200 rooting plantlets from one seedling. Then the plantlets were transferred to the soil and they grew under a foil tent. After five months of vegetation they were collected, dried and weighed. Chemical investigations of leaves of these plants were done. The vindoline and catharanthine were dominant alkaloids in the juvenile stage of plants (before blooming). Total amount of alkaloids, equal 2.95%, was gravimetrically determined in leaves of plants, after 4th passage, regenerated <i>in vitro</i> on NN medium supplemented with kinetin and IBA.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Somatic seeds of Plantago asiatica L.

2011 ◽  
Vol 75 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Emilia Andrzejewska-Golec ◽  
Joanna Makowczyńska
Keyword(s):  
Sodium Alginate ◽  
Calcium Chloride ◽  
Butyric Acid ◽  
Shoot Tips ◽  
Plantago Asiatica ◽  
First Time

Somatic seeds of <em>Plantago asiatica</em> L. were produced for the first time. Shoot-tips isolated from in vitro obtained 4-week shoots were encapsulated using sodium alginate and calcium chloride. Capsules with or without sucrose and with and without cytokinin - indole-3-butyric acid (IBA) were used. Sucrose presence in capsules very distinctly influences somatic seeds of <em>Plantago asiatica</em> germination and their conversion into plants. However, addition of IBA to capsules has not clear influence on the ability of plant regrowth. Plantlets transplanted to soil grew to phenotypically normal plants.

scholarly journals 317 Establishment and Multiplication of in Vitro Pyrus spp. Cultivars

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 446E-447
Author(s):  
Adriana Cibele de Mesquita Dantas ◽  
Adriano Nunes Nesi ◽  
Lilia Bender Machado ◽  
Janny Haerter ◽  
Gerson Renan de Luces Fortes
Keyword(s):  
Tissue Culture ◽  
Shoot Tips ◽  
Axillary Buds ◽  
Temperate Climate ◽  
In Vitro Multiplication ◽  
Growth Room ◽  
Sodium Hypochloride

The culture of meristems, shoot tips, and axillary buds leads to the method of in vitro multiplication that is easily used and safe to obtain uniform copies with no undesirable variations. This work aimed to propagate five in vitro pear cultivars: Housui, Carrick, Nijisseiki, Packham's Triumph, and Red Bartlett. The work was carried out in the Tissue Culture Laboratory at Embrapa Temperate Climate. The plants were sprayed with benomyl (1.0 mg./L) and agrimicine (2.4 mg/L) in the fields, 2 weeks before the shoots were collected. The shoots were then cut with two buds with no leaves and desinfested with alcohol 70% for 10 s and 1% sodium hypochloride for 20 min, 50 explants, 25 buds, and 25 meristems, were then transferred to test tubes containing MS salts and vitamins, myo-inositol (100.0 mg/L), sucrose (30.0 g/L), agar (6.0 g/L), added to in mg/L: BAP (1.0), GA3 (0.1), and NAA (0.01). Three pear cultivars were used for in vitro multiplication (`Nijisseiki', `Red Bartlett', and `Housui') by using the same basal salt with N reduced to strength, added to (in mg/L): BAP (1.6), NAA (0.16). The material was kept in growth room under 16-h photoperiod, 25 ± 2 °C and 19 μMol·m-2·s-1 of flux radiation. The in vitro contaminations were mainly due to bacteria derived from the bud material (71.5%). Higher oxidation for meristem material was observed for `Carrick' and `Packham`s Triumph'. `Red Bartlett' showed the best results for all the variable studied, although all cultivars in general presented low response.

scholarly journals Micropropagation of Centaurea macrocephala Pushk. ex Willd. by Shoot-axis Splitting

HortScience ◽  
1997 ◽  
Vol 32 (6) ◽  
pp. 1124-1125 ◽  
Author(s):  
Takashi Hosoki ◽  
Daisuke Kimura
Keyword(s):  
In Vitro Culture ◽  
Butyric Acid ◽  
Proliferation Rate ◽  
Axillary Buds ◽  
Culture Period

Micropropagation of Centaurea macrocephala Pushk. ex Willd. was achieved by subculturing of vertically split shoots and division of axillary buds on MS-based medium with 0.44 μm BA. A proliferation rate of 2.0 per 16-day culture period was obtained. Seventy percent of microcuttings obtained through in vitro culture could be rooted on a modified Hyponex medium with 25 μM IBA. All plantlets were readily acclimatized and grown in a greenhouse. Chemical names used: benzylaminopurine (BA); indole-3-butyric acid (IBA).

scholarly journals Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions

2019 ◽  
Vol 23 (3) ◽  
pp. 281-286 ◽  
Author(s):  
E. S. Bespalova ◽  
Yu. V. Ukhatova ◽  
N. N. Volkova ◽  
E. V. Oves ◽  
N. A. Gaitova ◽  
...  
Keyword(s):  
Shoot Tips ◽  
Axillary Buds ◽  
Regeneration Ability ◽  
Cultivation Conditions ◽  
Regeneration Rate ◽  
Dark Incubation ◽  
Potato Varieties ◽  
Droplet Vitrification ◽  
In Vitro Plants

Cryopreservation provides long-term storage of the gene pool of potato varieties in cryobanks at extremely low temperatures. Currently, droplet vitrification is the most widely used method for cryopreservation of potato varieties, which is constantly improving to increase the regeneration rates of the stored plant material. Different modifications of this method are used in the world’s leading potato genebanks. This paper presents the results of studying the effect of cultivation conditions after plunging into liquid nitrogen and thawing of shoots tips and axillary buds of in vitro plants on their postcryogenic recovery. The droplet-vitrification method modified at VIR was used for cryopreservation. The factor “prolonged dark incubation of explants” did not have a significant effect on the frequency of post-cryogenic regeneration of the studied varieties except for one variety (Krepysh), for which a significant increase in the regeneration rate was observed for the shoot tips cultivated in the darkness compared to the cultivation under the photoperiod 16/8 hours (light/darkness). The frequency of post-cryogenic regeneration of shoot tips was higher than that of the axillary buds for all varieties; however, these differences were significant (p < 0.05) only in two cases: for the variety Udacha (a photoperiod of 16/8 hours) and for the variety Krepysh (the dark incubation). The results of two-factor analysis of variance indicate that there is no effect of interaction of factor 1 (prolonged dark incubation) and factor 2 (explant type) on the ability of varieties to post-cryogenic recovery. Taking into account the obtained results, the further cryopreservation of an extended subset of 9 varieties was carried out using shoot tips, which, after freezing-thawing, were cultivated under the photoperiod of 16/8 hours. The frequency of post-cryogenic regeneration of these varieties varied from 30 to 60 %. A significant effect of genotype on postcryogenic recovery has been established. The ability of varieties to regenerate shoots after freezing and thawing was not related to the values of morphogenic indices of in vitro plants. The age of the meriklons (2–4 years) did not significantly affect either the morphogenic indices or the frequency of post-cryogenic regeneration.

scholarly journals In vitro plant regeneration from axillary buds of Hibiscus syriacus L.

2009 ◽  
Vol 36 (2) ◽  
pp. 174-178 ◽  
Author(s):  
Seo-Bum Jeon ◽  
Seung-Won Kang ◽  
Wan-Soon Kim ◽  
Gung-Pyo Lee ◽  
Sun-Hyung Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Axillary Buds ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant ◽  
Hibiscus Syriacus

scholarly journals Plant Regeneration from Axillary Buds of Triploid Watermelon (Citrullus vulgaris Schrad.)

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 472D-472
Author(s):  
S. Guzman ◽  
H. Alejandro ◽  
J. Farias ◽  
A. Michel ◽  
G. Lopez
Keyword(s):  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Axillary Buds ◽  
Ms Medium ◽  
Citrullus Vulgaris ◽  
Triploid Watermelon

Watermelon (Citrullus vulgaris Schrad.) is a widely grown crop throughout the tropics and subtropics. In Mexico, it is an economically important crop. In vitro adventitious shoot regeneration of watermelon has been reported from shoot tip culture, leaf, hypocotyl, and cotyledons. Hence, the objective of this study was to evaluate in vitro plant regeneration from axillary buds of triploid watermelon. Axillary buds explants were prepared from shoot of commercial cultivar in field of 60 old day plants. Explants of 2 to 3 mm were incubated 2 weeks on Murashige and Skoog (MS) shoot regeneration medium containing 2.5 mg/L kinetin (KT) or indole-3-butyric acid (IBA), or gibberellic acid (GA3), followed by 3 weeks on shoot elongation medium supplemented with different combinations of the same phytohormones. The percentage of explants (83% to 90%) that produced shoots, expansion in size of explant (0.81–1 cm) and shoot length (6 mm) were highest in MS medium containing KT or IBA. In the shoot elongation step, shoot length (0.9–1 cm) and leaves number (6–7) were highest in MS medium supplemented with 2.5 mg/L of KT or GA3 and 0.2 mg/L IBA, but the better induction of roots in elongated shoot occurred on MS medium with 2.5 mg/L KT and 0.2 mg/L IBA. The results show that axillary buds from watermelon is an alternative for the micropropagation of this crop.

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