scholarly journals In vitro cultures of Blackstonia perfoliata (L.) Hudson and an assay of the secoiridoid content

2014 ◽  
Vol 61 (3-4) ◽  
pp. 359-368 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Anna Krupińska ◽  
Barbara Thiem
Keyword(s):  
Medicinal Plant ◽  
Callus Tissue ◽  
In Vitro Cultures ◽  
Regenerated Plants

<i>In vitro</i> cultures of <i>Blackstonia perfoliata</i> (L.) Hudson, a popular medicinal plant, were initiated. Regenerated plants and callus tissue were obtained and then analysed for secoiridoids.

scholarly journals Callus culture and micropropagation of Eustoma grandiflorum Shinn.

2014 ◽  
Vol 57 (4) ◽  
pp. 423-430 ◽  
Author(s):  
Lutosława Skrzypczak ◽  
Maria Wesołowska ◽  
Barbara Thiem
Keyword(s):  
North America ◽  
Medicinal Plant ◽  
Callus Culture ◽  
Embryo Culture ◽  
Callus Tissue ◽  
Eustoma Grandiflorum ◽  
Regenerated Plants ◽  
Short Time ◽  
Eustoma Grandiflorum Shinn

Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of <em>Eustoma grandiflorum</em> Shinn. (<em>Gentianaceae</em>), a medicinal plant. This species, which does not naturally occur in this country, but is found in the southwestern regions of North America, can be propagated in vitro in a relatively short time. Regenerated plants were obtained in amounts enabling extensive phytochemical analyses to be conducted.

Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Endangered Medicinal Plant ◽  
Angelica Glauca

scholarly journals Results with the establishment of in vitro culture of Leucojum aestivum

2007 ◽  
Vol 13 (2) ◽  
Author(s):  
E. Kohut ◽  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Culture ◽  
In Vitro Cultures ◽  
Naphthalene Acetic Acid ◽  
Benzyl Adenine ◽  
Leucojum Aestivum ◽  
Leaves And Roots ◽  
Bulbous Plant

Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too.

A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE),A VALUABLE MEDICINAL PLANT

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
SUSANTA KUMAR MAITY
Keyword(s):  
Medicinal Plant ◽  
Leaf Shape ◽  
Basal Medium ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Hemidesmus Indicus ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.

scholarly journals The effect of media composition on multiplication of grape rootstocks in vitro

2012 ◽  
Vol 60 (8) ◽  
pp. 141-144 ◽  
Author(s):  
Břetislav Křižan ◽  
Eva Ondrušiková ◽  
Jana Moudrá
Keyword(s):  
Mass Production ◽  
Plant Material ◽  
In Vitro Cultures ◽  
Media Composition ◽  
Mass Propagation ◽  
Regenerated Plants ◽  
Optimized Protocol ◽  
Embryogenic Calluses ◽  
Murashige Skoog

The current demand for in vitro cultures of grape rootstocks, not only for mass production of plants, but also for genetic engineering is evident. The study on micropropagation of grape rootstock genotypes namely Kober 5BB, Kober 125AA and Teleki 5C was performed. The aim of the study was to develop an optimized protocol to obtain large quantity of plant material. Protocol is based on regeneration via organogenesis, considering that grape embryogenic calluses are laborious to establish and the genotype of the regenerated plants can be altered. Using of Driver and Kuniyuki Walnut media for the establishing of proliferating cultures gave better results than Murashige Skoog media in case of all used rootstocks. Subsequent cultivation on modified Murashige Skoog media with 1-naphtalene acetic acid and increased concentration of cytokynin was characterized by multiplication of cultures and formation of clusters with high multiplication capability. The clusters obtained from rootstock genotypes were suitable for mass propagation as well as for genetic transformation due to their high ability of regeneration.

HPTLC and HPTLC-coupled Bioassays for Bioprocess Control of Medicinal Plant In Vitro Cultures from the Balkan region

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381 ◽  
Author(s):  
E Wolfram ◽  
S Bräm ◽  
M Todorova ◽  
A Trendafilova ◽  
A Alvarez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
In Vitro Cultures ◽  
Bioprocess Control ◽  
Balkan Region ◽  
Plant In Vitro Cultures
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