scholarly journals Banding patterns in plant chromosomes. III. Dactylis glomerata subsp. aschersoniana (Graebner) Thell. from Poland

2014 ◽  
Vol 54 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Marta Mizianty
Keyword(s):  
Dactylis Glomerata ◽  
Banding Patterns ◽  
Plant Chromosomes

The present paper presents part of the author's studies on the variability of the genus <em>Dactylis</em> from Poland. The karyotypes and Giemsa heterochromatin banding patterns of three population samples of <em>Dactylis glomerata</em> subsp. <em>aschersoniana</em> (Graebner) Thell. (2n = 14) from Poland were studied in detail.

scholarly journals Banding patterns in plant chromosomes. II. Bibliography (1970-1980) - Anthophyta. Supplement 2

2014 ◽  
Vol 54 (3) ◽  
pp. 193-194 ◽  
Author(s):  
Marta Mizianty
Keyword(s):  
Plant Species ◽  
Banding Patterns ◽  
Plant Chromosomes

The present paper complements the main bibliography (8) and Supplement 1 (9). It contains list of 4 plant species and appropriate literature for it.

G and/or C-bands in plant chromosomes?

1984 ◽  
Vol 71 (1) ◽  
pp. 111-120
Author(s):  
I. Schubert ◽  
R. Rieger ◽  
P. Dobel
Keyword(s):  
Constitutive Heterochromatin ◽  
Giemsa Staining ◽  
Giemsa Banding ◽  
Base Pairs ◽  
Banding Patterns ◽  
C Banding ◽  
Plant Chromosomes ◽  
Nucleolus Organizing Region ◽  
Similarities And Differences ◽  
Simple Sequence

Similarities and differences become evident from comparisons of centromeric and non-centromeric banding patterns in plant and animal chromosomes. Similar to C and G-banding in animals (at least most of the reptiles, birds and mammals), centromeric and nucleolus-organizing region bands as well as interstitially and/or terminally located non-centromeric bands may occur in plants, depending on the kind and strength of pretreatment procedures. The last group of bands may sometimes be subdivided into broad regularly occurring ‘marker’ bands and thinner bands of more variable appearance. Non-centromeric bands in plants often correspond to blocks of constitutive heterochromatin that are rich in simple sequence DNA and sometimes show polymorphism; they thus resemble C-bands. However, most of these bands contain late-replicating DNA. Also they are sometimes rich A X T base-pairs, closely adjacent to each other and positionally identical to Feulgen+ and Q+ bands, thus being comparable to mammalian G-bands. Although banding that is reverse to the non-centromeric bands after Giemsa staining is still uncertain in plants, reverse banding patterns can be obtained with Feulgen or with pairs of A X T versus G X C-specific fluorochromes. It is therefore concluded that not all of the plant Giemsa banding patterns correspond to C-banding of mammalian chromosomes. Before the degree of homology between different Giemsa banding patterns in plants and G and/or C-bands in mammals is finally elucidated, the use of the neutral term ‘Giemsa band’, specified by position (e.g. centromeric, proximal, interstitial, terminal), is suggested to avoid confusion.

scholarly journals Analysis of CMA-DAPI bands and preparation of fluorescent karyotypes in thirty Indian cultivars of Lens culinaris

Caryologia ◽  
2021 ◽  
Author(s):  
Timir baran Jha ◽  
Biplab Kumar Bhowmick ◽  
Partha Roy
Keyword(s):  
Cost Effective ◽  
Fluorochrome Banding ◽  
Banding Patterns ◽  
Plant Chromosomes ◽  
Cost Effective Method ◽  
Production And Consumption ◽  
Breeding Programmes ◽  
Cultivated Species ◽  
Protein Rich ◽  
First Time

India holds a significant rank in production and consumption of the age old protein rich crop Lentil with only one cultivated species and a large number of phenotypically similar cultivars. The need for a reliable and cost effective method of genetic characterization to unravel differences within the Lentil cultivars was felt. The present paper adopted EMA based chromosome preparation followed by staining with two contrasting fluorochromes dyes CMA and DAPI that bind directly to GC and AT rich heterochromatic segments on chromosomes. Analysis of fluorochrome banding pattern furnished a comparative account of genetic diversity within the cultivars that could not be achieved by traditional karyotyping. The marker pair of nucleolar chromosomes (4th and 3rd, majorly) occupied a pivotal position to intensify differences between cultivars in terms of banding patterns around secondary constrictions, suggestive of  yet unknown variation in heterochromatin composition. Our study has strengthened genetic background and relationships of Lentil cultivars. We observed certain types of unusual fluorochrome bands that put forward the exclusivity of Indian germplasm and have questioned the mainstream heterochromatin elements of plant chromosomes captured by CMA-DAPI stains. The comprehensive fluorescent karyotypes of 30 L. culinaris cultivars prepared for the first time, serve as an archetype for the benefit of future breeding programmes in any Indian crop. 

Prevalence of Sitobion fragariae (Walker) over S. avenae (Fabricius) (Hemiptera: Aphididae) on wild cocksfoot grass (Dactylis glomerata) in south-east England

1990 ◽  
Vol 80 (1) ◽  
pp. 27-30 ◽  
Author(s):  
H. D. Loxdale ◽  
C. P. Brookes
Keyword(s):  
Dactylis Glomerata ◽  
Sitobion Avenae ◽  
Cereal Aphids ◽  
Banding Patterns ◽  
Sitobion Fragariae ◽  
South East England

AbstractIndividual cereal aphids of the species Sitobion avenae (Fabricius) and S. fragariae (Walker) were differentiated by their peptidase electrophoretic banding patterns; S. avenae has three diagnostic isoenzyme bands (PEP-3 to -5), whereas S. fragariae has one (PEP-1). Over 90% of 5389 individuals of these two species, collected from the grass Dactylis glomerata within 50 km of Rothamsted over the course of several years, were S. fragariae. The importance of D. glomerata and other non-cereal Gramineae species as alternative summer and overwintering hosts for S. avenae is discussed in relation to this finding.

scholarly journals Banding patterns in plant chromosomes. II. Bibliography (1970-1980)-Anthophyta

2014 ◽  
Vol 53 (1) ◽  
pp. 111-136 ◽  
Author(s):  
Marta Mizianty
Keyword(s):  
Banding Patterns ◽  
Plant Chromosomes

The bibliography deals mainly with papers published from 1970 until 1980. Some of them published before 1970 are also included. The list of periodicals, which served for this bibliography is given in Appendix 1.

scholarly journals Different Kinds of Heterochromatin in Higher Plant Chromosomes

1974 ◽  
Vol 14 (3) ◽  
pp. 499-504
Author(s):  
S. M. STACK ◽  
C. R. CLARKE ◽  
W. E. CARY ◽  
J. T. MUFFLY
Keyword(s):  
Chromosome Banding ◽  
Higher Plants ◽  
Pericentric Heterochromatin ◽  
Giemsa Staining ◽  
Higher Plant ◽  
Banding Patterns ◽  
Plant Chromosomes ◽  
Staining Techniques ◽  
Ornithogalum Virens ◽  
Basic Similarity

After the use of different Giemsa staining techniques, variations in chromosome banding patterns have often been observed in animal chromosomes. Such staining differences are usually interpreted to indicate that there is more than one type of heterochromatin in many animal chromosomes. Using two differential Giemsa staining techniques we have found different staining patterns in the chromosomes of two higher plants, Allium cepa and Ornithogalum virens. Furthermore, pericentric heterochromatin that occurs so commonly in animal chromo-somes was specifically Giemsa stained in O. virens. These results suggest the basic similarity of higher plant and animal chromosomes.

scholarly journals Banding patterns in plant chromosomes. II. Bibliography (1970-1980) - Anthophyta. Supplement 1

2014 ◽  
Vol 53 (4) ◽  
pp. 485-487 ◽  
Author(s):  
Marta Mizianty
Keyword(s):  
Plant Species ◽  
Banding Patterns ◽  
Plant Chromosomes

The present paper complements the main bibliography (6). It contains list of 24 plant species and appropriate literature for it.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

1986 ◽  
Vol 44 ◽  
pp. 150-151
Author(s):  
M.K. Lamvik ◽  
L.L. Klatt
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Staining Pattern ◽  
Banding Patterns ◽  
Mass Thickness ◽  
New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

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