scholarly journals Formation and development of sperms in angiosperms

2014 ◽  
Vol 50 (1-2) ◽  
pp. 99-101 ◽  
Author(s):  
S. Tatintseva
Keyword(s):  
Pollen Tube ◽  
Delivery System ◽  
Pollen Grain ◽  
Female Gamete ◽  
Sperm Cells ◽  
Angiosperm Species ◽  
Different Levels ◽  
Full Maturity

Spermiogelnesis has been studied in a large number of Angiosperm species characterizing different levels of phylogenetic system. The formation and development of male gamets can be described as formation of sperm cells that undergo ontogenesis which stimulates changes up to full maturity of the pollen grain. The process of ontogenesis is aimed at creating a suitable delivery system by the pollen tube to a female gamete.

Actin During Pollen Germination

1986 ◽  
Vol 86 (1) ◽  
pp. 1-8
Author(s):  
J. HESLOP-HARRISON ◽  
Y. HESLOP-HARRISON ◽  
M. CRESTI ◽  
A. TIEZZI ◽  
F. CIAMPOLINI
Keyword(s):  
Medium Release ◽  
Pollen Tube ◽  
Vegetative Cell ◽  
Pollen Germination ◽  
Pollen Grain ◽  
Actin Microfilaments ◽  
Vegetative Cells ◽  
Stage Of Development ◽  
Angiosperm Species

The cytoplasm of the vegetative cell of the ungerminated pollen grain of Endymton non-scriplus and other angiosperm species contains numerous fusiform bodies sometimes exceeding 15μm in length and 2.5 μm in width, which bind fluorescent-labelled phalloidin and are likely therefore to constitute a storage form of actin. The bodies are dispersed during the activation of the pollen, being replaced by aggregates of slender phalloidin-binding fibrils, which converge towards the germination apertures and are present in the emerging pollen tube. The storage bodies appear to be homologous with crystalline-fibrillar structures, shown in an earlier paper to be abundantly present in the vegetative cells of Nicotiana pollen. These are composed of massive aggregates of linearly disposed units with individual widths of 4–7 nm, probably to be interpreted as actin microfilaments. Vegetative-cell protoplasts from mature but ungerminated pollen disrupted in osmotically balancing medium release extended phalloidin-binding fibrils of a kind not observed in the intact grain. It is suggested that these are derived by the rapid dissociation of the compact actin storage bodies present in the vegetative cell at this stage of development.

scholarly journals Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm

2015 ◽  
Vol 112 (43) ◽  
pp. 13378-13383 ◽  
Author(s):  
Hua Jiang ◽  
Jun Yi ◽  
Leonor C. Boavida ◽  
Yuan Chen ◽  
Jörg D. Becker ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Intercellular Communication ◽  
Protein Phosphatase ◽  
Pollen Tube Growth ◽  
Vegetative Cell ◽  
Male Gametophyte ◽  
Pollen Grain ◽  
Sperm Cells ◽  
Coding Region

An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5′ UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.

scholarly journals The Long Journey of Pollen Tube in the Pistil

2018 ◽  
Vol 19 (11) ◽  
pp. 3529 ◽  
Author(s):  
Yang-Yang Zheng ◽  
Xian-Ju Lin ◽  
Hui-Min Liang ◽  
Fang-Fei Wang ◽  
Li-Yu Chen
Keyword(s):  
Pollen Tube ◽  
Female Gametophyte ◽  
Male Gametophyte ◽  
Pollen Grain ◽  
Tubular Structure ◽  
Future Research ◽  
Sperm Cells ◽  
Double Fertilization ◽  
Complex Process ◽  
Pass Through

In non-cleistogamous plants, the male gametophyte, the pollen grain is immotile and exploits various agents, such as pollinators, wind, and even water, to arrive to a receptive stigma. The complex process of pollination involves a tubular structure, i.e., the pollen tube, which delivers the two sperm cells to the female gametophyte to enable double fertilization. The pollen tube has to penetrate the stigma, grow in the style tissues, pass through the septum, grow along the funiculus, and navigate to the micropyle of the ovule. It is a long journey for the pollen tube and its two sperm cells before they meet the female gametophyte, and it requires very accurate regulation to perform successful fertilization. In this review, we update the knowledge of molecular dialogues of pollen-pistil interaction, especially the progress of pollen tube activation and guidance, and give perspectives for future research.

scholarly journals Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kazuki Motomura ◽  
Hidenori Takeuchi ◽  
Michitaka Notaguchi ◽  
Haruna Tsuchi ◽  
Atsushi Takeda ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Pollen Tube ◽  
Sperm Cell ◽  
Pollen Tubes ◽  
Vegetative Nucleus ◽  
Sperm Cells ◽  
Apical Region ◽  
Basal Region ◽  
Specific Expression ◽  
Directional Growth

AbstractDuring the double fertilization process, pollen tubes deliver two sperm cells to an ovule containing the female gametes. In the pollen tube, the vegetative nucleus and sperm cells move together to the apical region where the vegetative nucleus is thought to play a crucial role in controlling the direction and growth of the pollen tube. Here, we report the generation of pollen tubes in Arabidopsis thaliana whose vegetative nucleus and sperm cells are isolated and sealed by callose plugs in the basal region due to apical transport defects induced by mutations in the WPP domain-interacting tail-anchored proteins (WITs) and sperm cell-specific expression of a dominant mutant of the CALLOSE SYNTHASE 3 protein. Through pollen-tube guidance assays, we show that the physiologically anuclear mutant pollen tubes maintain the ability to grow and enter ovules. Our findings provide insight into the sperm cell delivery mechanism and illustrate the independence of the tip-localized vegetative nucleus from directional growth control of the pollen tube.

scholarly journals Pollen tube incompatibility reaction on the stigma in selfpollinated Sinapis alba L.

2014 ◽  
Vol 65 (1-2) ◽  
pp. 101-105 ◽  
Author(s):  
Renata Śnieżko ◽  
Krystyna Winiarczyk
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Sinapis Alba ◽  
Pollen Grain ◽  
Tube Growth ◽  
Sinapis Alba L ◽  
Growth Changes ◽  
Incompatibility Reaction

After selfpollination of <em>Sinapis alba</em> L. pollen tubes growth is inhibited on the stigma. The pollen grains germinate 3-4 hours after pollination. The pollen give rise to one or more pollen tubes. They grow along the papillae. In the place of contact between the papilla and pollen tube the pellicula is digested. Then the direction of pollen tube growth changes completely. Pollen tubes grow back on the exine of their own pollen grain, or turn into the air. The pollen tubes growth was inhibited in 6-8 hours after selfpollination. After crosspollination usually there is no incompatibility reaction.

Sugar composition of pollen grain and pollen tube cell walls

1981 ◽  
Vol 20 (5) ◽  
pp. 981-984 ◽  
Author(s):  
Norio Nakamura ◽  
Hiroshi Suzuki
Keyword(s):  
Pollen Tube ◽  
Cell Walls ◽  
Pollen Grain ◽  
Sugar Composition ◽  
Tube Cell

scholarly journals Nuclear activity of sperm cells during Hyacinthus orientalis L. in vitro pollen tube growth

2010 ◽  
Vol 62 (3) ◽  
pp. 1255-1269 ◽  
Author(s):  
Krzysztof Zienkiewicz ◽  
Anna Suwińska ◽  
Katarzyna Niedojadło ◽  
Agnieszka Zienkiewicz ◽  
Elżbieta Bednarska
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Sperm Cells ◽  
Nuclear Activity ◽  
Hyacinthus Orientalis
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