scholarly journals Alkylresorcinols of rye (Secale cereale L.) caryopses IV. Three-step preparation of 5-n-alkylresorcinols

2015 ◽  
Vol 47 (4) ◽  
pp. 379-389 ◽  
Author(s):  
W. Mejbaum-Katzenellenbogen ◽  
F. Tłuścik ◽  
A. Kozubek
Keyword(s):  
Thin Layer ◽  
Secale Cereale ◽  
Room Temperature ◽  
Uv Spectrum ◽  
Wheat Grains ◽  
Alkyl Derivatives ◽  
Secale Cereale L ◽  
Spectrum Characteristic ◽  
Layer Chromatography ◽  
Derivatives Of

Whole rye or wheat grains were extracted with acetone at room temperature. The extracts were pooled and acetone was evaporated. The remaining acetone oil was washed out with n-pentane until a colourless filtrate was obtained. The residue was dried, then dissolved in chloroform-acetone (95:5) mixture and separated by column chromatography on silica gel. The fractions containing 5-n-alkylresorcinols were pooled, condensed and crystallised from n-hexane. The preparation obtained was homogeneous in thin-layer chromatography and gave the UV spectrum characteristic for 5-n-alkyl derivatives of resorcinol.

scholarly journals Alkylresorcinols in rye (Secale cereale L.) grains. I. Micromethod for determination of alkyl derivatives of resorcinol in rye grain

2015 ◽  
Vol 44 (4) ◽  
pp. 479-489 ◽  
Author(s):  
W. Mejbaum-Katzenellenbogen ◽  
F. Tłuścik ◽  
A. Kozubek ◽  
A. Sikorski
Keyword(s):  
Preparative Thin Layer Chromatography ◽  
Uv Spectrum ◽  
Selective Determination ◽  
Alkyl Derivatives ◽  
Secale Cereale L ◽  
Spectrum Characteristic ◽  
Acetone Extracts ◽  
Layer Chromatography ◽  
Derivatives Of

A pure preparation of alkylresorcinol from rye grains was obtained by preparative thin-layer chromatography, which gave a UV spectrum characteristic for 5-n-alkylresorcinols and orcinol. This preparation served as standard in the elaboration of a micro method (for alkylresorcinols determination in acetone extract from rye grain. It was found that this method is suitable for selective determination of 5-n-alkylrezorcinols in acetone extracts from rye grains.

scholarly journals Alkylresorcinols in rye (Secale cereale L.) grains. VI. Colorimetric micromethod for the determination of alkylresorcinols with the use of diazonium salt, Fast Blue B

2014 ◽  
Vol 50 (4) ◽  
pp. 645-651 ◽  
Author(s):  
Franciszek Tłuścik ◽  
Arkadiusz Kazubek ◽  
Wanda Mejbaum-Katzenellenbogen
Keyword(s):  
Secale Cereale ◽  
Diazonium Salt ◽  
Precise Method ◽  
Fast Blue ◽  
Alkyl Derivatives ◽  
Secale Cereale L ◽  
Derivatives Of

A simple and precise method for alkylresorcinols determination was elaborated. The method a based on coupling of alkylresorcinals with diazotized Fast Blue B, in acidified propaaiol. The method is highly specific for 5-n-alkyl derivatives of resorcinols. In case of rye and wheat 98.1% and 96.2% respectively of total extinction value were due to 5-n-alkylresorcinols.

scholarly journals SINTESIS CALKON ((E)-1,3-DI(NAPHTHALEN-2-YL)PROP-2-EN-1-ONE) DAN AKTIVITASNYA SEBAGAI ANTIOKSIDAN

2012 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Rahmiwati Hilma ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Free Radical ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Intermediate Compound ◽  
Alkaline Condition ◽  
Point Measurement ◽  
Anti Inflammatory ◽  
Layer Chromatography

Study on chalcone calkon (E)-1,3-di(naphthalen-2-yl)prop-2-en-1-one synthesis have been carried out with stirrer methode. These compounds can be used as intermediate compound to synthesize others compounds which was reported having antimicrobial, anti-inflammatory, anti-depressant, anti-tumour. The of chalcones synthesis vatives were reported in acid and alkali condition. In this study, chalcone and its derivates were synthesized by using stirrer method in alkaline condition in room temperature. the compounds subjected to somes analyses including melting point measurement, thin layer chromatography and HPLC. Scavenging free radical by using DPPH methode showed Scavenging free radical with LC50 >80 μg/ml min potent activity while the ascorbat acid LC50 89,79 μg/ml.

Single-reagent polarization fluoroimmunoassay for barbiturates in urine.

1984 ◽  
Vol 30 (11) ◽  
pp. 1765-1769 ◽  
Author(s):  
D L Colbert ◽  
D S Smith ◽  
J Landon ◽  
A M Sidki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescence Polarization ◽  
Room Temperature ◽  
Cross Reactivity ◽  
Screening Techniques ◽  
Standard Curve ◽  
Layer Chromatography

Abstract We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, results in an assay with a broad cross-reactivity spectrum for most common barbiturates. One adds urine to the pre-mixed reagent, incubates the solution for a few minutes at room temperature, and measures the fluorescence polarization. The tracer/antiserum reagent is stable for at least six months at 4 degrees C. Results compare favorably with two other commonly used screening techniques for barbiturates, thin-layer chromatography and the EMIT-stTM (Syva) system. Although designed as a qualitative screen, some drugs can be quantified by means of a standard curve of the relevant barbiturate.

Instability of Some TWchothecenes in Methanol

1986 ◽  
Vol 69 (5) ◽  
pp. 902-903 ◽  
Author(s):  
Ru-Dong Wei ◽  
Fun S Chu
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Early Stage ◽  
Acetoxy Group ◽  
Layer Chromatography

Abstract Stability of 8 trichothecenes stored in methanol at room temperature was studied by thin layer chromatography. Results indicate that the trichothecenes which bear an acetoxy group at both C3 and C4 are very susceptible to methanolysis. In the early stage, the acetoxy group at C3 is most favorable for the transesterification. All trichothecenes tested were transformed to several products after prolonged (22 days) exposure to methanol.

scholarly journals The reaction of iodobenzene-p-sulphonyl chloride (pipsyl chloride) with certain amino acids and peptides, and with insulin

1967 ◽  
Vol 102 (3) ◽  
pp. 815-824 ◽  
Author(s):  
J. C. Fletcher
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Cysteic Acid ◽  
Buffer System ◽  
The Common ◽  
Layer Chromatography ◽  
Derivatives Of

1. A system of separation using buffered Celite columns is described that enables the pipsyl derivatives of most of the common amino acids to be separated. 2. The reaction of pipsyl chloride with several amino acids not included in previous studies has been investigated. In particular, knowledge of the acid-soluble pipsyl derivatives of arginine, histidine, lysine, tyrosine and cysteic acid has been extended. 3. Reproducible factors have been obtained that enable corrections to be applied for the breakdown of pipsylamino acids on acid hydrolysis. 4. The reaction of pipsyl chloride with peptides has been studied under various conditions. 5. The extent of the reaction between pipsyl chloride and insulin depends on the nature of the solvent-buffer system, and under the best conditions so far found is about 75% complete. 6. In an Appendix, the separation of pipsylamino acids by thin-layer chromatography is described.

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