scholarly journals The role of plant growth substances in the regulation of the cell cycle in antheridial filaments of Chara vulgaris L. I. Effect of gibberellic acid on some, processes in the course of the cell cycle

2015 ◽  
Vol 46 (2) ◽  
pp. 317-329 ◽  
Author(s):  
Mirosław Godlewski
Keyword(s):  
Cell Cycle ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Substance ◽  
Similar Degree ◽  
Chara Vulgaris ◽  
Cell Dimensions ◽  
Dividing Cells ◽  
Plant Growth Substances

The effect of gibberellic acid (10<sup>-4</sup> M) on the incorporation of 8-<sup>14</sup>C adenine, <sup>3</sup>H phenylalanine, the dimensions of mitotic cells and the durations of particular stages in the cell cycle were studied in synchronously dividing cells of the antheridial filaments in <i>Chara vulgaris</i> L. during succesive periods of growth and differentiation. GA<sub>3</sub> strongly stimulates the uptake of both labeled precursors in the course of a whole interphase and in all generations of the antheridial filaments; approximatively in proportion to the intensity of the process in the control. The gibberellin causes a slight increment in cell dimensions and strongly reduces the cell cycle durations: the S, G2, and M to a similar degree. The earlier is the generation of the antheridial filament, the more pronounced is the influence of the plant growth substance. Since the gibberellin stimulated the course of all examined processes, the present study did not reveal any stage of interphase to be especially sensitive to GA<sub>3</sub>. The results suggest to interpret the effect of GA<sub>3</sub> as an unspecific stimulator of metabolism in cells of the antheridial filaments of <i>Chara vulgaris</i> L.

scholarly journals RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage–induced cell senescence

2016 ◽  
Vol 27 (8) ◽  
pp. 1346-1357 ◽  
Author(s):  
Pavol Cekan ◽  
Keisuke Hasegawa ◽  
Yu Pan ◽  
Emily Tubman ◽  
David Odde ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Dna Repair ◽  
Cell Cycle Progression ◽  
Nucleotide Exchange ◽  
Normal Cells ◽  
Cytoplasmic Transport ◽  
Dividing Cells ◽  
Ran Gtp

The coordination of cell cycle progression with the repair of DNA damage supports the genomic integrity of dividing cells. The function of many factors involved in DNA damage response (DDR) and the cell cycle depends on their Ran GTPase–regulated nuclear–cytoplasmic transport (NCT). The loading of Ran with GTP, which is mediated by RCC1, the guanine nucleotide exchange factor for Ran, is critical for NCT activity. However, the role of RCC1 or Ran⋅GTP in promoting cell proliferation or DDR is not clear. We show that RCC1 overexpression in normal cells increased cellular Ran⋅GTP levels and accelerated the cell cycle and DNA damage repair. As a result, normal cells overexpressing RCC1 evaded DNA damage–induced cell cycle arrest and senescence, mimicking colorectal carcinoma cells with high endogenous RCC1 levels. The RCC1-induced inhibition of senescence required Ran and exportin 1 and involved the activation of importin β–dependent nuclear import of 53BP1, a large NCT cargo. Our results indicate that changes in the activity of the Ran⋅GTP–regulated NCT modulate the rate of the cell cycle and the efficiency of DNA repair. Through the essential role of RCC1 in regulation of cellular Ran⋅GTP levels and NCT, RCC1 expression enables the proliferation of cells that sustain DNA damage.

scholarly journals Comparison of the duration of the cell cycle in successive generation of synchronously dividing antheridial filaments of Chara vulgaris L. as measured with 3H thymidine

2015 ◽  
Vol 42 (1) ◽  
pp. 121-131 ◽  
Author(s):  
M. Godlewska ◽  
M. J. Olszewska
Keyword(s):  
Cell Cycle ◽  
Successive Generation ◽  
Chara Vulgaris ◽  
Dividing Cells ◽  
S Period

Duration of the cell cycle in synchronously dividing cells of successive generation of antheridial filaments in <i>Chara vulgaris</i> L. was estimated on-the basis of labeling with <sup>3</sup>H thymidine. Duration of the cell cycle is proportional to the volume of cells and with their decreasing in the consequence1 of consecutive divisions, the cell cycle becomes shorter. In the 2-, 4-, 8-, and 16-cell generations the length of the S period remains constant, so the duration of the G2 period is gradually reduced.

scholarly journals Roles of plant growth substance in callus induction of Achyranthes bidentata

2016 ◽  
Vol 6 ◽  
Author(s):  
Hongying Duan ◽  
Weikai Ding ◽  
Jianying Song ◽  
Jiaming Xu ◽  
Huina Wang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Callus Induction ◽  
Growth Substance ◽  
Optimum Combination ◽  
Dichlorophenoxyacetic Acid ◽  
Growth Substances ◽  
Plant Growth Substance ◽  
Achyranthes Bidentata ◽  
Plant Growth Substances ◽  
2,4 Dichlorophenoxyacetic Acid

<p>    <strong>In this research, callus from leaves, petioles and stems of <em>Achyranthes bidentata</em> was evidently initiated by plant growth substance, in which 2,4-dichlorophenoxyacetic acid (2,4-D) was very important to callus induction, but effects of other plant growth substances were various, and the optimum combination of plant growth substances for callus induction from leaves, petioles and stems was respectively obtained. Compared with callus induction from leaves and petioles, callus induction from stems was easier, and the higher induction rate and bigger mass of callus from stems were obtained. This study showed that the dedifferentiation capacity</strong><strong> of various explants from </strong><strong><em>Achyranthes bidentata</em></strong><strong> was obviously different, and effects of plant growth substance on callus induction from various explants of <em>Achyranthes bidentata</em> were significantly diverse.</strong><strong></strong></p>

scholarly journals Influence of the light factor on the course of the cell cycle in the successive generations of the antheridial filaments of Chara vulgaris L.

2015 ◽  
Vol 46 (1) ◽  
pp. 31-45 ◽  
Author(s):  
J. Maszewski
Keyword(s):  
Cell Cycle ◽  
Hormonal Regulation ◽  
Continuous Light ◽  
Continuous Illumination ◽  
Chara Vulgaris ◽  
Inhibition Of Growth ◽  
Successive Generations ◽  
Role Of Light ◽  
Kinetics Of

The exposure to continuous light causes a rise of the mitotic index in the successive generations of the antheridial filaments of <i>Chara vulgaris</i>, whereas culture in darkness leads to a considerable depression of mitosis, and if prolonged, to its complete cessation. Light reverses the effect of the depression of mitosis caused by darkness: in the 16-, and 32-cell generations mitotic activity reappeares as early as after 3 h, but in the 2-, 4-, and 8-cell generations only after 18 h. The size of the cells in the antheridial filaments of plants exposed to continuous illumination is larger as compared with the control material. After being kept in darkness the cells become smaller. The analysis of the size of the latter suggests the inhibition of growth in initial and final periods of interphase. In all generations continuous light reduces the duration of the cell cycle while darkness protracts it. The duration of S phase is similar in all generations of antheridial filaments (ca. 16 h) and is not modified by the light conditions. Thus, the light factor seems to interfere mainly with G2 phase, and its role in the regulation of the cell cycle is correlated with the duration of this phase. The role of light in the kinetics of the cell cycle of the particular generations of antheridial filaments in Chara is discussed in the aspect of the nutritional requirements of cells, hormonal regulation and phytochrome.

scholarly journals Alleviating Role of Gibberellic Acid in Enhancing Plant Growth and Stimulating Phenolic Compounds in Carrot (Daucus carota L.) under Lead Stress

2021 ◽  
Vol 13 (21) ◽  
pp. 12329
Author(s):  
Muhammad Awais Ghani ◽  
Muhammad Mehran Abbas ◽  
Basharat Ali ◽  
Rukhsanda Aziz ◽  
Rashad Waseem Khan Qadri ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Daucus Carota ◽  
Daucus Carota L ◽  
Chlorophyll Contents ◽  
Pb Stress

Toxicity of heavy-metals in soil is a major constraint for the production of carrots (Daucus carota L.). Different plant growth regulators are being used to overcome this problem. It has been found that plant growth regulators induce stress tolerance in plants. In this study, the role of exogenously applied plant growth regulator, gibberellic acid (GA3) was examined in soil grown two carrot cultivars under four different levels of lead (0, 50, 100, and 150 mg/kg) with one level of gibberellic acid (50 ppm). Results showed that Pb stress retarded the plant growth and reduced chlorophyll contents in the leaves of both carrot cultivars. A significant decrease was observed in photosynthetic attributes by Pb addition alone. However, exogenously applied GA3 ameliorated the plant growth and chlorophyll contents in the leaves of both carrot cultivars under Pb stressed conditions. Moreover, GA3 also decreased the uptake of Pb concentration in carrot leaves and roots. In addition, GA3 significantly regulated the phenolic compounds concentration in both carrot cultivars under Pb stress. In this study, cultivar T-29 was found to be more tolerant to Pb stress, however, cultivar Mevarick experienced higher damage regarding plant growth under Pb stress.

Sign in / Sign up

Export Citation Format

Share Document