scholarly journals Changes in the electrophoretic pattern of glucosidases during apple seeds stratification

2015 ◽  
Vol 42 (2) ◽  
pp. 193-199 ◽  
Author(s):  
A. Podstolski ◽  
St. Lewak
Keyword(s):  
Electrophoretic Pattern ◽  
Triphenyltetrazolium Chloride ◽  
Apple Seed ◽  
Electrophoretic Patterns ◽  
Seed Stratification ◽  
Apple Seeds

The technique of localization of glucosidases on disc electropherograms, based on reduction of 2,3,5-triphenyltetrazolium chloride by enzymatically liberated sugar is described. The changes in electrophoretic patterns of amygdaline, phloridzin and p-nitrophenyl-β-glucoside hydrolysing glucosidases during apple seed stratification were studied. The changes were correlated with earlier described changes in endogenous apple seed amygdaline and phloridzin contents.

scholarly journals The Effect Of Bioinsecticide Granule Mixture Of Sugar Apple Seed (Annonae Squamosa) And Betel Leaf (Piper Betle) On Morphology Of Eggs Aedes Aegypti

2021 ◽  
Vol 9 (1) ◽  
pp. 26-30
Author(s):  
Firdha Firdha Novitasari ◽  
Dwi Wahyuni Dwi Wahyuni ◽  
Erma Sulistyaningsih Erma Sulistyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Public Health Problem ◽  
Ordinal Regression ◽  
Seed Extract ◽  
Control Group ◽  
Piper Betle ◽  
Betel Leaf ◽  
Apple Seed ◽  
Regression Test ◽  
Apple Seeds

Introduction: Dengue hemorrhagic fever (DHF) is an important public health problem in Indonesia because its morbidity and mortality are quite high. Prevention by vector control methods with chemical insecticides adversely affects the environment. Research on granular bioinsectide mixture of betel leaf extract and sugar apple seeds against Aedes aegypti is expected to be used to break the chain of Aedes aegypti mosquitoes starting  from the juvenile stage  (eggs) as an effort to control the vector population naturally. Objective: This study aims to analyze the effect of granule bioinsecticide mixture of sugar apple seed extract and betel leaf on changes in the morphology of Aedes aegypti eggs. Methods: This study is a true-experimental study with a Post test Only Control Group Design design. The treatment group will be given a granular bioinsecticide, a mixture of sugar apple seed extract and betel leaf, positive control will be given abate while negative control will be given aquades. Each group uses 25 eggs with 3 repetitions. On day 3, all eggs will be observed using a stereo microscope to determine morphological changes. The data obtained will be analyzed using ordinal regression test. Results: the ordinal regression test got the value of ρ in the Wald test between (0.012 - 0.955)> α = 0.01. Conclusion: there is no effect of mixed granules of betel leaf and sugar apple seeds on the mortality of Aedes aegypti eggs. Further research is needed on new formulations using other natural ingredients to increase the effect of ovicides on Aedes aegypti.

scholarly journals Human ‘creatine kinase conversion factor’ identified as a carboxypeptidase

1984 ◽  
Vol 221 (2) ◽  
pp. 465-470 ◽  
Author(s):  
R J Edwards ◽  
D C Watts
Keyword(s):  
Creatine Kinase ◽  
Conversion Factor ◽  
Electrophoretic Pattern ◽  
Beta Subunits ◽  
Rabbit Muscle ◽  
Activity Profile ◽  
Muscle Creatine Kinase ◽  
Electrophoretic Patterns ◽  
Carboxypeptidase N ◽  
Low Activity

The effect of partially purified ‘creatine kinase conversion factor’ on rabbit muscle creatine kinase is shown to be that of a carboxypeptidase, removing the C-terminal lysine residue from both subunits. These changes fully explain the three-banded electrophoretic patterns of the partially and the fully modified rabbit and human enzymes. The factor also produces a similar electrophoretic pattern with haemoglobin A; comparison with the effects of carboxypeptidases A and B permits the inference that the C-terminal residues of both alpha- and beta-subunits are removed. Small synthetic peptides are poor or non-substrates. A low activity with hippuryl-L-lysine may be due to contamination of the preparation with carboxypeptidase N. The possibility has been excluded that the action of conversion factor on creatine kinase involves modification of the protein thiol groups. Mr, substrate-specificity, pH-activity profile and the effects of metal ions distinguish creatine kinase conversion factor from carboxypeptidases A, B and N. On the basis of this evidence it is proposed to give the conversion factor the provisional name of carboxypeptidase K.

Electrophoretic patterns of proteinuria in feline spontaneous chronic kidney disease

2019 ◽  
Vol 22 (2) ◽  
pp. 114-121
Author(s):  
Marco Giraldi ◽  
Saverio Paltrinieri ◽  
Paola Scarpa
Keyword(s):  
Chronic Kidney Disease ◽  
At Risk ◽  
Molecular Weight ◽  
Kidney Disease ◽  
Sodium Dodecyl ◽  
Electrophoretic Pattern ◽  
Low Molecular Weight ◽  
Tubular Proteinuria ◽  
Exact Test ◽  
Electrophoretic Patterns

Objectives The purpose of this study was to describe the electrophoretic patterns of proteinuria in cats at risk of and cats with chronic kidney disease (CKD), and to investigate whether the presence of high-molecular-weight (HMW) and low-molecular-weight (LMW) proteins were associated with CKD, proteinuria and/or disease progression. Methods Healthy cats at risk of developing renal disease (n = 17) and cats affected with CKD at different stages (n = 22) were prospectively enrolled and sampled over time. Seventy urine samples were included and assayed with a commercially available sodium dodecyl sulfate–agarose gel electrophoresis (SDS-AGE) method. Each sample (gel lane) was inspected to identify albumin, HMW and LMW proteins, and an electrophoretic pattern (albuminuria, glomerular, tubular, mixed or negative) was assigned accordingly. Fisher’s exact test was used to assess the distribution of HMW and LMW proteins in cats grouped according to International Renal Interest Society stage and to the magnitude of proteinuria, and to assess if HMW and LMW proteins at the time of inclusion were associated with the development and progression of CKD. Results In samples of cats at risk, the most common pattern was glomerular (84.6%); glomerular pattern was also common in cats with CKD (54.2%), although mixed proteinuria and tubular proteinuria were also present (29.5% and 11.4%, respectively). The presence of LMW proteins was associated with CKD ( P <0.0001) and to a urine protein:creatinine ratio >0.2 ( P = 0.025). Both HMW and LMW proteins were not associated with progression of CKD within 6 months (n = 14). Conclusions and relevance Our results showed that HMW proteinuria is common in healthy cats at risk of developing CKD, although the pathological significance needs to be confirmed. The detection of LMW proteins in urine of cats suspected to be affected by CKD, especially in non-azotaemic, non-proteinuric or borderline proteinuric cats, suggests the presence of kidney damage.

scholarly journals Detection of serum proteins in the electrophoretic patterns of total proteins of mycoplasma cells

1976 ◽  
Vol 77 (2) ◽  
pp. 189-198 ◽  
Author(s):  
O. E. Yaguzhinskaya
Keyword(s):  
Culture Medium ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Sucrose Density Gradient ◽  
Total Proteins ◽  
Molecular Weights ◽  
Electrophoretic Patterns ◽  
Mycoplasma Cell ◽  
Co Precipitation ◽  
Serum Precipitate

SummaryThe contamination of mycoplasma cell preparations by serum proteins originating from culture medium was studied.A. laidlawiiandM. arthritidiscells were grown in the presence of [14C]-aminoacids, and the cells were washed with 0·9% NaCl by threefold centrifugation. Total proteins of the washed cells were analysed by SDS gel electrophoresis. Coomassie-stained electrophoretic patterns were compared with autoradiographs of the same gels. The stained electrophoretic pattern of washedA. laidlawiigrown without serum was identical with autoradiographs of the same cells grown without or with serum. That of washedA. laidlawiigrown with serum differed from the corresponding autoradiograph by the presence of extra protein bands I, II, III, and IV with molecular weights of over 160,000, 80,000–87,000, 55,000 and 25,000, respectively. The same extra bands were found in stained electrophoretic patterns of washed: (a)A. laidlawiicells grown without serum and mixed with serum in the stationary phase, (b)M. arthritidiscells, as compared with their autoradiographs, (c) serum precipitate. The bands III and IV may be due to the heavy and light chains of γ-globulin, the band II might belong to transferrin or to some component of complement. Acidification of serum to pH 5 brought about 100-fold rise of amount of serum precipitate, the number of bands in the electrophoretic pattern of the precipitate being also increased. Stained electrophoretic patterns of cells purified by twofold centrifugation in step sucrose density gradient (1·20–1·27 g./cm.3forA. laidlawii,and 1·15–1·25 forM. arthritidis) contained no extra bands and matched completely with their autoradiographs.It was concluded that contamination of washed mycoplasma cells by serum proteins is mainly due to co-precipitation of aggregated serum proteins together with cells during centrifugation rather than to adsorption of serum proteins on the cell surface.

Proximate Composition of the Apple Seed and Characterization of Its Oil

2007 ◽  
Vol 3 (5) ◽  
Author(s):  
Xiuzhu Yu ◽  
Frederick R. van de Voort ◽  
Zhixi Li ◽  
Tianli Yue
Keyword(s):  
Amino Acid ◽  
Proximate Composition ◽  
Animal Feed ◽  
Value Added ◽  
Proximate Analysis ◽  
Apple Seed ◽  
Protein Components ◽  
Sulfur Containing ◽  
Apple Seeds

Apple seeds, a common byproduct of apple processing, have been examined for their overall proximate composition, fatty acid and amino acid composition of the lipid and protein components, respectively, as well as their key mineral constituents. Proximate analysis indicated that apple seeds are rich in oil content and protein ranging from 27.5 to 28% and 33.8 to 34.5% respectively, comparing favorably with oilseeds. GC analysis indicated high levels of linoleic acid (~49%) with the other dominant fatty acids being oleic, palmitic and stearic acids, ranging from ~39, 7 and 2% respectively. Amino acid analysis indicates that there are substantial amounts of sulfur containing amino acids in the apple seed. The apple seeds also contain significant amounts of phosphorus, potassium, magnesium, calcium and iron, in the order of 720, 650, 510, 210 and 110 mg/100g, respectively. Based on the proximate composition of the apple seeds, if adequate amounts are available as a process byproduct, apple seeds could have value-added potential as a source of edible oil, with the oil cake potentially serving as an animal feed supplement.

scholarly journals Variety-specificity of soluble proteins of potato tubers

2015 ◽  
Vol 43 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Marta Czupryn ◽  
Kazimierz Toczko
Keyword(s):  
Potato Tuber ◽  
Genetic Research ◽  
Electrophoretic Pattern ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
The Other ◽  
Potato Tubers ◽  
Genetic Origin ◽  
Electrophoretic Patterns ◽  
Taxonomic Studies

Separation of soluble tuber proteins from six potato clones and twelve varieties cultivated in Poland has been accomplished by disc electrophoresis. It was found that electrophoretic pattern was unique for a given clone or variety. Data obtained confirm results of the other authors for the other varieties and indicate that electrophoretic analysis of potato tuber proteins can be a useful method for taxonomic studies. Such analysis however cannot be used for genetic research since no correlations were found between electrophoretic patterns and genetic origin of respective clones and varieties.

scholarly journals Effect of Pollinators on Production of Hybrid Apple Seed

2015 ◽  
Vol 15 (4) ◽  
pp. 363
Author(s):  
Ljubomir Radoš ◽  
Dragana Novaković
Keyword(s):  
High Impact ◽  
Hybrid Seed ◽  
Apple Seed ◽  
Fruit Species ◽  
Artificial Pollination ◽  
Hybrid Seeds ◽  
New Varieties ◽  
Apple Seeds

Apple is an open pollinated entomofilic fruit species. For successful fruiting, it is necessary to provide two or more varieties which are good pollinators, in addition to being compatible with the main cultivar. The situation is similar with the artificial pollination of apples with the aim of obtaining hybrid seeds, the only difference is that for this kind of pollination insects do not have to be provided. In order to create new varieties of apples, we selected the varity Ajdared as the recipient of pollen (mother) and Topaz, Rubies and Pink Lady, as pollinators varieties for which we do not have data how they act as pollinators or what kind of impact they have on the production of hybrid seed. Based on the obtained data, we concluded that the varieties pollinators in all combinations have a high impact on the production of hybrid apple seeds

Changes in proteins of Domiati cheese during pickling

1972 ◽  
Vol 39 (2) ◽  
pp. 219-225 ◽  
Author(s):  
M. H. Abd El-Salam ◽  
Safinaz El-Shibiny
Keyword(s):  
Amino Acids ◽  
Room Temperature ◽  
Gel Filtration ◽  
Electrophoretic Pattern ◽  
Pasteurized Milk ◽  
Molecular Weights ◽  
Electrophoretic Patterns ◽  
Related Substances ◽  
Nitrogenous Substances

SummaryDomiati cheese from homogenized and unhomogenized pasteurized milk was pickled for 4 months at room temperature. During pickling, the soluble tyrosine and tryptophan contents of the cheese gradually increased, indicating progressive proteolysis.Gel filtration of cheese extract on Sephadex G-25 revealed the presence of nitrogenous substances of various molecular weights. The maximum formation of amino acids and related substances was observed after 15 days storage.The changes in the electrophoretic patterns of proteins in the cheese during pickling indicated that both αs - and β-caseins were attacked. β-Casein, however, was much less affected.Homogenization was found to affect the formation of soluble nitrogenous substances, but was without effect on the electrophoretic pattern of the protein in the cheese.

Electrophoresis of serum protein to detect alpha 1-antitrypsin deficiency: five illustrative cases.

1985 ◽  
Vol 31 (8) ◽  
pp. 1397-1399 ◽  
Author(s):  
R Malfait ◽  
F Gorus ◽  
C Sevens
Keyword(s):  
Serum Protein ◽  
Visual Inspection ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
The Other ◽  
Electrophoretic Patterns ◽  
Antitrypsin Deficiency ◽  
Alpha 1 Antitrypsin Deficiency ◽  
Alpha 1 Antitrypsin

Abstract We describe five cases of severe alpha 1-antitrypsin (AAT) deficiency to illustrate the importance of visual inspection of electrophoretic patterns of serum proteins. In four patients the diagnosis of AAT deficiency was clinically unsuspected; in the other patient, the electrophoretic pattern was the first clue to confirm the diagnosis. Densitometric scanning of these patterns invariably overestimated the concentration of alpha 1-globulin. By visually inspecting electrophoretic strips instead of relying on densitometry, clinical chemists can help detect AAT deficiency earlier.

scholarly journals GENE DOSAGE AND THE EXPRESSION OF ELECTROPHORETIC PATTERNS IN HETEROPLOID MOUSE CELL LINES

Genetics ◽  
1973 ◽  
Vol 74 (3) ◽  
pp. 521-531
Author(s):  
Rosann A Farber
Keyword(s):  
Cell Lines ◽  
Isocitrate Dehydrogenase ◽  
Gene Dosage ◽  
Electrophoretic Pattern ◽  
Mouse Cell ◽  
Chromosome 7 ◽  
Glucosephosphate Isomerase ◽  
Electrophoretic Patterns ◽  
The Relationship

ABSTRACT Spontaneously transformed mouse cell lines heterozygous for electrophoretic markers have been studied to determine the relationship between gene dosage and phenotype. It is shown that a clone with an electrophoretic pattern for glucosephosphate isomerase of three bands in a ratio of 4A:4AB:1B contains three copies of chromosome 7, which carries the gene for this enzyme. A clone from a different line with a pattern of three bands in a ratio of 1A:6AB:9B for NADP-isocitrate dehydrogenase has four copies of the chromosome carrying this gene or three copies plus a rearrangement which apparently involves this chromosome. These results show that all of the alleles for each enzyme are expressed to an equal extent in these cells.

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