scholarly journals Phosphorus compounds, proteins, nuclease and acid phosphatase activities in isolated spinach chloroplasts

2015 ◽  
Vol 41 (2) ◽  
pp. 217-222 ◽  
Author(s):  
E. Mikulska ◽  
W. Maciejewska-Potapczyk ◽  
I. Konopska ◽  
B. Damsz
Keyword(s):  
Acid Phosphatase ◽  
Total Protein ◽  
Phosphorus Compounds ◽  
Spinach Chloroplast ◽  
Simple Method ◽  
Intact Chloroplasts ◽  
Chloroplast Isolation ◽  
Chloroplast Fraction ◽  
Very High ◽  
Isolated Chloroplasts

This paper deals with attempts to elaborate a simple method of spinach chloroplast isolation ensuring a high proportion of intact chloroplasts. We obtained 3 preparations of isolated chloroplasts. Several preliminary analyses of the obtained chloroplast fraction were also performed. Phosphorus compounds, total protein and the enzyme activities of RNase, DNase and GPase were determined. We found: 0,36-0,59% of RNA, 0,19-0,24% of DNA, 2,1-2,9% of phospholipids and 26-28% of protein. RNase activity was very high.

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

1989 ◽  
Vol 35 (10) ◽  
pp. 2127-2129 ◽  
Author(s):  
Y Bergqvist ◽  
L Karlsson ◽  
L Fohlin
Keyword(s):  
Breast Milk ◽  
Total Protein ◽  
Brilliant Blue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Simple Method ◽  
Coomassie Brilliant Blue ◽  
Kjeldahl Method ◽  
Coomassie Brilliant

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

Inhibition of Photosynthetic Reactions by Aureomycin

1984 ◽  
Vol 39 (6) ◽  
pp. 627-633 ◽  
Author(s):  
Ji-yu Ye ◽  
U. Heber
Keyword(s):  
Membrane Potential ◽  
Proton Gradient ◽  
Effective Inhibitor ◽  
Mesophyll Protoplasts ◽  
Electrochromic Shift ◽  
Low Concentrations ◽  
Intact Chloroplasts ◽  
Photosynthetic Reactions ◽  
Isolated Chloroplasts

The effect of aureomycin on photosynthesis was investigated. This antibiotic which has been reported to stimulate photosynthesis at very low concentrations is an effective inhibitor at higher concentrations. In mesophyll protoplasts and isolated chloroplasts from spinach, 50% inhibition of CO2 reduction required about 20 μᴍ aureomycin. The reduction of 3-phosphoglycerate and of oxaloacetate by intact chloroplasts was also inhibited, but not that of nitrite and methylviologen which was actually stimulated. NADP reduction by broken chloroplasts and methylviologen- dependent photophosphorylation were also sensitive to aureomycin. The electrochromic shift at 518 nm which indicates formation of a light-dependent membrane potential was suppressed in the presence of 200 μᴍ aureomycin and the transthylakoid proton gradient was decreased. The data confirm reports that aureomycin has uncoupling properties, and they indicate that it also acts as an inhibitor of ferredoxin/NADP reductase.

scholarly journals Detection & Classification of Cardiac Arrhythmia

2017 ◽  
Vol 6 (1) ◽  
pp. 31
Author(s):  
Chetan M. Jadhav ◽  
V. K. Bairagi
Keyword(s):  
Cardiac Arrhythmia ◽  
Early Stage ◽  
Low Cost ◽  
Simple Method ◽  
Arrhythmia Detection ◽  
Ecg Signals ◽  
Electrocardiogram Signals ◽  
Electrical Impulses ◽  
Very High

<p>The term Arrhythmia refers to any change from the normal sequence in the electrical impulses. It is also treated as abnormal heart rhythms or irregular heartbeats. The rate of growth of Cardiac Arrhythmia disease is very high &amp; its effects can be observed in any age group in society. Arrhythmia detection can be done in many ways but effective &amp; simple method for detection &amp; diagnosis of  Cardiac Arrhythmia is by doing analysis of Electrocardiogram signals from ECG sensors. ECG signal can give us the detail information of heart activities, so we can use ECG signals to detect the rhythm &amp; behaviour of heart beats resulting into detection &amp; diagnosis of Cardiac Arrhythmia. In this paper new &amp; improved methodology for early Detection &amp; Classification of Cardiac Arrhythmia has been proposed. In this paper ECG signals are captured using ECG sensors &amp; this ECG signals are used &amp; processed to get the required data regarding heart beats of the human being &amp; then proposed methodology applies for Detection &amp; Classification of Cardiac Arrhythmia. Detection of Cardiac Arrhythmia using ECG signals allows us for easy &amp; reliable way with low cost solution to diagnose Arrhythmia in its prior early stage.</p>

Transformation of Rana tigrina during Progressive Metamorphosis

2021 ◽  
Vol 30 (1) ◽  
pp. 102-109
Author(s):  
Dhananjay Mishra ◽  
K.Venu Achari
Keyword(s):  
Acid Phosphatase ◽  
Total Protein ◽  
Ribonucleic Acid ◽  
Fold Increase ◽  
Total Activity ◽  
Total Protein Content ◽  
Total Carbohydrate ◽  
Rna Content ◽  
Rana Tigrina ◽  
Kinetics Of

We determined the kinetics of metamorphosis, apoptosis, and tail regression in Rana tigrina. Acid phosphatase activity (µMole Pi.hr-1.tail-1) in the growing and regressing tail attended six to thirty fold increase respectively. However total activity in the trunk was decreased through progressive growing stages of metamorphosis. Total protein content in the trunk of tadpoles at climax stage (XXI) was decrease (35%) from 2.6mg/ml to 1.7mg/ml. The tail of tadpole tissue has shown a two fold increase in total Ribonucleic Acid (RNA) content from stage III to stage XVIII. But there was again decrease in total RNA content at climax stage (stage XXI). This might be possible due to decreased protein synthetic status. When the experiment was performed in trunk homogenate the amount of total carbohydrate (mg/ml) was slightly increased from 37mg/ml to 38.6mg/ml. this might be due to increase in the activity of α-amylase enzymes in the viscera of developing tadpole when it reached the climax stage.

Acid and alkaline phosphatases in the lymphomyeloid tissues of elasmobranch fish

1978 ◽  
Vol 58 (3) ◽  
pp. 727-730 ◽  
Author(s):  
Ragnar Fänge
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
High Activity ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Alkaline Phosphatases ◽  
Large Numbers ◽  
Alkaline Reaction ◽  
Elasmobranch Fish ◽  
Very High

Activities of phosphomonoesterases were measured at acid and at alkaline reaction (pH 4–5 or 9–65) in homogenates of elasmobranch tissues especially lymphomyeloid structures. The animals were dogfish (Scyliorhinus caniculd) and two species of ray (Raja brachyura, R. naevus). Acid phosphatase activity was high in the epigonal tissue, Leydig's organ, the spleen and the thymus. High activity was also found in the pancreas and the kidney, whereas skeletal and cardiac muscle showed low values. The activity of alkaline phosphatase was very high in the kidney and relatively low in other tissues. Ultrasonification of homogenates from the dogfish resulted in increase of acid phosphatase activity but had little effect on alkaline phosphatase activity. The high activity of acid phosphatase in lymphomyeloid tissue may be due to the presence of large numbers of various types of leucocytes.

Slow secondary fluorescence kinetics associated with the onset of photosynthetic carbon assimilation in intact isolated chloroplasts

1984 ◽  
Vol 220 (1220) ◽  
pp. 327-338 ◽  
Keyword(s):  
Carbon Assimilation ◽  
Assay Medium ◽  
Exponential Increase ◽  
Photosynthetic Carbon ◽  
Intact Chloroplasts ◽  
Dark Interval ◽  
Photosynthetic Carbon Assimilation ◽  
Isolated Chloroplasts ◽  
Fast Rates ◽  
Secondary Fluorescence

Experiments with carefully isolated, largely intact chloroplasts, capable of fast rates of CO 2 -dependent O 2 evolution, show that the fall in chlorophyll a fluorescence (from the early maxima reached immediately after illumination) is interrupted by a ‘shoulder’ which is associated with the exponential increase in the rate of O 2 evolution. The length of this induction period was increased by storage, by decreased temperature, by increased orthophosphate concentration in the assay medium or by the presence of D, L-glyceraldehyde. It could also be shortened by the addition of 3-phosphoglycerate or dihydroxyacetonephosphate. In each treatment the shoulder in fluorescence shifted so that the association with the period of exponential increase was maintained. When illumination was re-started after a short dark interval, induction was minimal and no shoulder could be discerned, but both the lag in the onset of O 2 evolution and the shoulder were restored when the chloroplasts were resuspended in fresh assay medium during the period of darkness. The relation between chlorophyll a fluorescence and the onset of photosynthetic carbon assimilation is discussed.

scholarly journals Lymphokine-activated killer cells in rats. III. A simple method for the purification of large granular lymphocytes and their rapid expansion and conversion into lymphokine-activated killer cells.

1988 ◽  
Vol 167 (1) ◽  
pp. 15-29 ◽  
Author(s):  
N L Vujanovic ◽  
R B Herberman ◽  
A A Maghazachi ◽  
J C Hiserodt
Keyword(s):  
Nk Cells ◽  
Rapid Expansion ◽  
Mononuclear Leukocytes ◽  
Target Cells ◽  
Large Granular Lymphocytes ◽  
Lymphokine Activated Killer Cells ◽  
Simple Method ◽  
Killer Cells ◽  
Very High ◽  
Granular Lymphocytes

A simple method for the purification and rapid expansion of large granular lymphocytes into cells with efficient broad antitumor cytotoxicity after stimulation by human rIL-2 is described. Nylon-wool nonadherent splenic mononuclear leukocytes from Fischer 344 rats were cultured in medium containing 1,000 U/ml rIL-2. The initial response of a small subpopulation of cells (less than 2%) to rIL-2 was their adherence to the plastic surface. This response was noted as soon as 2 h after addition of rIL-2. 2-h rIL-2-activated plastic adherent lymphocytes were 90-98% LGL, expressed surface markers characteristic of rat NK cells (OX8 [CD8]+, asialo GM1, laminin+, OX19 [CD5]-, R1-3B3 [CD5]-, W3/25 [CD4]-, OX39 [CD25]-, Ia-, and Ig-), and expressed very high levels of cytotoxicity against YAC-1 target cells. In addition to the above markers, plastic-adherent LGLs obtained at 24, 48, or 72 h progressively expressed Ia surface antigens, but were not phagocytic and contained less than 1% monocytes/macrophages by morphology. When 24- or 48-h plastic-adherent LGL/NK cells were cultured over 3-4 d in rIL-2, the cells expanded between 30- and 100-fold, reaching densities between 2-3 X 10(6) cells/ml. These rapidly expanding LGL/NK cells also generated very high levels of LAK activity (including lysis of fresh NK-resistant solid tumor cells), expressed a phenotype characteristic of activated rat NK/LAK cells, and incorporated [3H]TdR into DNA. This technique not only provides a novel method for the purification of LGL/NK cells for in vitro studies but also provides a means for the rapid expansion of highly purified cells with high levels of broad antitumor (LAK) cytotoxicity.

73. Phosphorus Compounds of Milk. VI. The Effect of Heat on Milk Phosphatase. A Simple Method for Distinguishing Raw from Pasteurised Milk, Raw from Pasteurised Cream, and Butter made from Raw Cream from that made from Pasteurised Cream

1933 ◽  
Vol 5 (1) ◽  
pp. 63-74 ◽  
Author(s):  
H. D. Kay ◽  
W. R. Graham
Keyword(s):  
Quantitative Determination ◽  
Raw Milk ◽  
Test Tube ◽  
Phosphorus Compounds ◽  
Simple Method ◽  
Heated Milk ◽  
Degree Of Certainty ◽  
Qualitative Test ◽  
Fair Degree

A method is described for the quantitative determination of phosphatase in raw milk. Using this method, it has been found that phosphatase is sufficiently thermolabile to be destroyed completely by pasteurisation if this process is properly carried out. The absence of phosphatase from a sample of milk or cream indicates with a fair degree of certainty that the milk or cream has been heated sufficiently to destroy such pathogenic organisms as were originally present, though it is of course no guarantee that the product is free from these organisms at the time of testing.A simple, qualitative test-tube method is described which may be used for differentiating between raw and heated milk, or between raw and heated cream, and which with slight modifications may also be used for distinguishing between butters made from raw or from heated cream.

A comparison of the sites of acid phosphatase activity in an adult filaria, Setaria sp. and in some gastro -intestinal nematodes

Parasitology ◽  
1980 ◽  
Vol 81 (3) ◽  
pp. 603-608 ◽  
Author(s):  
Jun Maki ◽  
Toshio Yanagisawa
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Alimentary Canal ◽  
Body Wall ◽  
The Body ◽  
Luminal Surface ◽  
Toxocara Cati ◽  
Similar Activity ◽  
Very High

SUMMARYThe histochemical localization of acid phosphatase in an adult filaria, Setaria sp. obtained from the peritoneal cavity of a cow was closely examined and compared with that of adult nematodes parasitic in the host alimentary canal; special attention was paid to the intestine and body wall of the parasites. Setaria sp. was found to show high acid phosphatase activity in the interchordal hypodermis of the body wall and uterine microfilariae, and similar activity is suspected to occur in the cuticle. The intestine of this nematode exhibited very low, if any, activity. In contrast, nematodes parasitic on the alimentary canal, such as Toxocara cati, T. canis, Physaloptera sp. and Ancylostoma caninum, showed no activity in the body wall and very high activity in the luminal surface of their intestine. The possible function of the abundant acid phosphatase in the body wall of this filaria is discussed.

Sign in / Sign up

Export Citation Format

Share Document