scholarly journals Morphological and Molecular Characterization of Entomophthorales (Entomophthoromycota: Entomophthoromycotina) from Argentina

2020 ◽  
Vol 55 (2) ◽  
Author(s):  
Romina G. Manfrino ◽  
Louela A. Castrillo ◽  
Claudia C. López Lastra ◽  
Andrea V. Toledo ◽  
Walter Ferrari ◽  
...  
Keyword(s):  
Molecular Data ◽  
Molecular Techniques ◽  
Accurate Identification ◽  
Fungal Isolates ◽  
Unidentified Species ◽  
First Time ◽  
Aphid Host ◽  
Morphological And Molecular Characterization ◽  
Molecular Characters

We characterized 17 insect-pathogenic entomophthoralean fungal isolates (Entomophthoromycotina: Entomophthorales) using morphological and molecular techniques. We identified four species from various insect hosts: (<strong><em>i</em></strong>) <em>Entomophthora planchoniana</em>, six specimens from aphids; (<strong><em>ii</em></strong>) <em>Pandora </em><em>neoaphidis</em>, three specimens from aphids; (<strong><em>iii</em></strong>) <em>Zoophthora phalloides </em>from an aphid; and (<strong><em>iv</em></strong>) <em>Z. radicans</em>, seven specimens from insects in the orders Diptera, Hemiptera, and Lepidoptera. Analysis of ITS1 data from <em>E. planchoniana </em>showed clustering in accordance to aphid host species. <em>Entomophthora planchoniana </em>from <em>Macrosiphum</em><em> </em><em>euphorbiae </em>clustered together, separate from the isolate from <em>Myzus persicae</em>. The <em>P.</em><em> </em><em>neoaphidis </em>specimens clustered with sequences from other aphid-pathogenic <em>Pandora </em>species in GenBank. In this study, <em>Z. phalloides </em>from <em>Brevicoryne brassicae </em>and <em>Z. radicans </em>from an unidentified species of Chironomidae (Diptera) in Argentina were characterized for the first time. The present study was initiated to elucidate the taxonomy of the entomophthoralean fungi in Argentina according to their morphological and molecular characters. The presented results emphasize the significance of the combination of molecular data and information on morphology, ecology, and host range for accurate identification of entomophthoralean and allied genera.

Morphological and molecular characterization of adult worms of Leucochloridium paradoxum Carus, 1835 and L. perturbatum Pojmańska, 1969 (Digenea: Leucochloridiidae) from the great tit, Parus major L., 1758 and similarity with the sporocyst stages

2013 ◽  
Vol 88 (4) ◽  
pp. 506-510 ◽  
Author(s):  
I. Rząd ◽  
P. Hofsoe ◽  
R. Panicz ◽  
J.K. Nowakowski
Keyword(s):  
Parus Major ◽  
Morphological Characters ◽  
Great Tit ◽  
Molecular Techniques ◽  
Morphological Identification ◽  
The Baltic Sea ◽  
The Baltic ◽  
Morphological And Molecular Characterization ◽  
Sea Coast

AbstractUnlike the sporocyst stages, adult leucochloridiid digeneans are difficult to differentiate. Sporocyst broodsacs can be identified on the basis of their colour and banding pattern, but in the absence of broodsacs and when experimental infection cannot be performed, tentative morphological identification needs to be verified, and molecular techniques offer a tool to do this. In this study, adult leucochloridiid digeneans were collected from the great tit (Parus major) found dead at three localities at or near the Baltic Sea coast (Hel, Bukowo-Kopań and Szczecin) in northern Poland. On the basis of differences in their morphological characters, Hel specimens were tentatively assigned to Leucochloridium perturbatum, Bukowo-Kopań and Szczecin specimens being identified tentatively as L. paradoxum. Subsequent ribosomal DNA sequence analysis confirmed the identification of these leucochloridiid flukes. Nucleotide sequences discriminating between the two species were identical to those used by earlier authors as characteristic of two distinctly different sporocyst broodsacs representing L. perturbatum and L. paradoxum.

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

2020 ◽  
Author(s):  
Hend H.A.M. Abdullah ◽  
Dina Aboelsoued ◽  
Tarek K Farag ◽  
Kadria N Abdel Megeed ◽  
Sobhy Abdel-Shafy ◽  
...  
Keyword(s):  
Molecular Techniques ◽  
Ixodid Ticks ◽  
Morphological Identification ◽  
Vector Borne Diseases ◽  
Maldi Tof ◽  
Vector Borne ◽  
First Time ◽  
Multiple Pathogens ◽  
Apparently Healthy

Abstract Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

scholarly journals Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

2017 ◽  
Vol 66 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Beata Zimowska ◽  
Ewa Dorota Zalewska ◽  
Ewa Dorota Król ◽  
Agnieszka Furmańczyk
Keyword(s):  
Culture Media ◽  
Culture Collection ◽  
Morphological Similarity ◽  
Reference Isolate ◽  
Angelica Archangelica ◽  
Its Regions ◽  
Standard Culture ◽  
First Time ◽  
Morphological And Molecular Characterization

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.

An extraordinary new genus and three new species of Acostemmini (Hemiptera: Cicadellidae: Deltocephalinae) from Madagascar with comments on the morphology and classification of the tribe

Zootaxa ◽  
2012 ◽  
Vol 3209 (1) ◽  
pp. 28 ◽  
Author(s):  
J. N. ZAHNISER ◽  
M. W. NIELSON
Keyword(s):  
New Species ◽  
New Genus ◽  
Strong Support ◽  
Molecular Data ◽  
Morphological Characterization ◽  
New Combination ◽  
Speci Mens ◽  
Three New Species ◽  
First Time

The circumscription and morphological characterization of the deltocephaline tribe Acostemmini is revised based on dis-coveries of new and poorly known taxa from recent collections in Madagascar and from examination of the type speci-mens of Alocoelidia fulva Evans, Iturnoria insulana Evans, and Protonesis delegorguei Spinola. Alocoelidia Evans 1954,Caelidioides Signoret 1880, Iturnoria Evans 1954, and Protonesis Spinola 1850 were previously placed in Coelidiinaebut were later removed from the subfamily and until now have been unplaced to subfamily are placed here in Deltocepha-linae: Acostemmini, new placement. A new genus and species, Ikelibeloha cristata gen. n., sp. n., are described that havean unusually modified structure of the head and a large, crested pronotum. Molecular data (28S, Histone H3 genes) wereobtained for Ikelibeloha and Iturnoria, and analyzed with other members of Deltocephalinae. Results of the phylogeneticanalyses show strong support for the monophyletic clade ((Acostemma, Eryapus), (Ikelibeloha, Iturnoria)) and thus a re-lationship between previously described Acostemmini, Ikelibeloha, and Iturnoria has strong statistical support, and aclose relationship is inferred between these and the morphologically similar Alocoelidia, Caelidioides, and Protonesis.Two new species of Alocoelidia, A. maurae sp. n. and A. chasei sp. n. are described, and the genus is redescribed. Themale genitalia of Caelidioides tristis (Signoret), Iturnoria insulana, Alocoelidia fulva, and Acostemella rubra Evans areillustrated and described for the first time. The female genitalia of A. fulva, C. tristis and I. insulana are described and thefirst and second valvulae of C. tristis are illustrated. The habitus and face of Protonesis delegorguei are illustrated and thegenus is redescribed. The characters traditionally used to define Acostemmini are reviewed with notes on their known de-grees of variation, and characters that differentiate Acostemmini from the closely related tribe Stegelytrini are discussed.The type of Malagasiella minima Evans was also examined, and Malagasiella Evans, 1954 is considered a junior syn-onym of Doratulina Melichar, 1903 (Deltocephalinae: Stenometopiini), syn. n. giving the new combination Doratulina minima (Evans) comb. n.

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

2012 ◽  
Vol 57 (3) ◽  
Author(s):  
Kateřina Huňová ◽  
Martin Kašný ◽  
Vladimír Hampl ◽  
Roman Leontovyč ◽  
Aleš Kuběna ◽  
...  
Keyword(s):  
Czech Republic ◽  
Molecular Data ◽  
Reproductive Organs ◽  
Shell Morphology ◽  
Snail Population ◽  
Intermediate Hosts ◽  
The Czech Republic ◽  
Trematode Infections ◽  
First Time

AbstractLymnaeid snails of the genus Radix serve as intermediate hosts of some schistosomes and fasciolids. In Europe, delineation of species within the genus Radix is unresolved and, therefore, spectrum of snail hosts susceptible to trematode infections is under discussion. We used and compared three criteria for species delineation using snails collected at 43 localities. (a) Sequence analysis of ITS-2 rDNA disclosed that the collected snails belong to four species — R. auricularia (Linnaeus, 1758), R. peregra (Müller, 1774), R. lagotis (Schrank, 1803) and R. labiata (Rossmaessler, 1835) (criteria and names are based on the work of Bargues et al. 2001). Occurrence of R. peregra in the Czech Republic was confirmed by molecular data for the first time. (b) Characterization of reproductive system disclosed differences in location, size and shape of bursa copulatrix and its ductus. Unfortunately, some R. labiata specimens shared morphological features of reproductive organs with R. lagotis. (c) Statistical analysis of shell morphology proved that significant differences exist among particular species. One prediction model showed that correct classification of species may be achieved in 82-84% of cases. However, identification of individual snails in the field (without knowledge of respective snail population and use of statistical tools) still remains a complicated issue due to overlaps of shell characteristics. Concerning the role in trematode transmission, R. lagotis, R. labiata and R. peregra are susceptible to Trichobilharzia regenti. Also, successful experimental infections of R. lagotis and R. labiata by Fascioloides magna were accomplished.

scholarly journals Molecular characterization of three species belongs to the Allocreadioidea, Hemiuroidea and Plagiorchioidea (Platyhelminthes: Trematoda) infecting freshwater fishes in India

2016 ◽  
Vol 53 (4) ◽  
pp. 378-384 ◽  
Author(s):  
A. Chaudhary ◽  
S. Mukut ◽  
H. S. Singh
Keyword(s):  
Phylogenetic Reconstruction ◽  
Freshwater Fishes ◽  
Molecular Data ◽  
Trematode Species ◽  
Rrna Gene ◽  
28S Rrna ◽  
Channa Punctata ◽  
Digenetic Trematodes ◽  
First Time

Summary Three species of digenetic trematodes are redescribed based on specimens collected from the intestine of freshwater fishes of Hastinapur and Meerut (U.P.), India: Allocreadium handiai (Pande, 1937) Madhavi, 1980 (Allocreadioidea: Allocreadiidae) from Mystus tengara (Hamilton, 1822) (Siluri-formes: Bagridae), Genarchopsis goppo Ozaki, 1925 (Hemiuroidea: Derogenidae) and Phyllodistomum chauhani Motwani & Srivastava, 1961 (Plagiorchioidea: Gorgoderidae) from Channa punctata (Bloch, 1793) (Perciformes: Channidae). The three species were subjected to morphological, morphometric and molecular analyses. The morphological study revealed that A. handiai, G. goppo and P. chauhani can be distinguished by their congeners on the basis of their morphology. Partial nucleotide sequences of the 28S ribosomal RNA gene were obtained from the three trematode species and deposited in the GenBank. A phylogenetic reconstruction based on the 28S rRNA gene placed the three studied species within their respective families and their validity is discussed. For the first time molecular data of newly collected material of these species from India were used for confirmation of their validity and to assess their phylogenetic relationships.

scholarly journals Isolation and Molecular Identification of some Fungi Associated with Jatropha curcas (L.)

2021 ◽  
Vol 37 (2) ◽  
pp. 103-112
Author(s):  
N.G Iyany ◽  
A.E Ataga
Keyword(s):  
Jatropha Curcas ◽  
Rhizopus Oryzae ◽  
Fungal Species ◽  
Molecular Techniques ◽  
Accurate Identification ◽  
Universal Primer ◽  
Aspergillus Tamarii ◽  
Jatropha Curcas L ◽  
Fungal Isolates ◽  
Aspergillus Sp

Jatropha curcas is a plant of great economic importance that experiences high incidence of fungal attack. Misidentification of the fungal species is bound to occur with the use of traditional cultural methods where organisms are identified morphologically and/or microscopically. This study was carried out to isolate and identify the fungi associated with Jatropha curcas (L.) using both traditional/ cultural techniques and molecular methods. The fungi were isolated from diseased leaves and stems of J. curcas using both Standard Blotter and Potato Dextrose Agar (PDA) methods. DNA was extracted from the fungal isolates using Zymo Fungal/Bacteria DNA MiniPrep Kit. Amplification of the Internal Transcribed Spacer (ITS) regions of the fungal isolates was carried out using fungi universal primer pairs for ITS4 and ITS5. The amplicons were sequenced and the isolates were identified as Penicillium brevicompactum, Aspergillus sp., Botryosphaeria rhodina, Aspergillus nomius, Aspergillus tamarii, Rhizopus oryzae, Penicillium citrinum and Fusarium solani. Phylogenetic analysis was carried out to know the relationship between the isolates and other closely-related species in GenBank. Jatropha curcas is colonized by many fungal species some of which may be pathogenic to the plant, and molecular techniques pose the best alternative for accurate identification of these organisms. Keywords: Jatropha curcas, fungi, polymerase chain reaction, phylogeny, sequencing

scholarly journals Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (O3[ratio ]K6 and O4[ratio ]K68) in Thailand and India

2000 ◽  
Vol 125 (1) ◽  
pp. 17-25 ◽  
Author(s):  
N. R. CHOWDHURY ◽  
S. CHAKRABORTY ◽  
B. EAMPOKALAP ◽  
W. CHAICUMPA ◽  
M. CHONGSA-NGUAN ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Time Frame ◽  
Molecular Techniques ◽  
Dna Fingerprint ◽  
Gene Characterization ◽  
Geographical Regions ◽  
Similar Restriction ◽  
First Time ◽  
Close Genetic Relationship

Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4[ratio ]K68 serovar for the first time in March 1998 alongside the continued predominant incidence of the O3[ratio ]K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3[ratio ]K6 and O4[ratio ]K68 strains and for comparison, non-O3[ratio ]K6 and non-O4[ratio ]K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3[ratio ]K6 and O4[ratio ]K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of strains yielded nearly similar restriction fragment length polymorphism (RFLP) patterns for the O3[ratio ]K6 and O4[ratio ]K68 isolates from Calcutta and Thailand. Phylogenetic analysis of the NotI RFLP showed that the O3[ratio ]K6 and O4[ratio ]K68 strains formed a cluster with 78–91% similarity thus indicating close genetic relationship between the two different serovars isolated during the same time-frame but from widely separated geographical regions. The non-O3[ratio ]K6 and non-O4[ratio ]K68, in contrast, showed different ribotype, AP-PCR and PFGE patterns.

scholarly journals Morphological and molecular characterization of Xiphinema species from Shenzhen, China

2016 ◽  
Vol 53 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Y. Zeng ◽  
W. Ye ◽  
Z. Zhang ◽  
H. Sun ◽  
L. Yong ◽  
...  
Keyword(s):  
Acacia Mangium ◽  
Distinct Species ◽  
Morphological Characters ◽  
Molecular Data ◽  
Biodiversity Survey ◽  
Close Relationship ◽  
Nematode Biodiversity ◽  
Morphological And Molecular Characterization ◽  
Partial 18S

Summary During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunaniense Wang & Wu, 1992, X. brasiliense Lordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.

scholarly journals Morphological and Molecular Characterization of Endophytic Fungi Associated with Cocoa (Theobroma cacao L.) in India

2020 ◽  
pp. 1-8
Author(s):  
M. Chaithra ◽  
S. Vanitha ◽  
A. Ramanathan ◽  
V. Jegadeeshwari ◽  
V. Rajesh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Endophytic Fungi ◽  
Tamil Nadu ◽  
Fungal Endophytes ◽  
Molecular Techniques ◽  
Universal Primers ◽  
Malt Extract ◽  
Plant Parts ◽  
Morphological And Molecular Characterization

Aim: To identify cocoa associated endophytic fungi through morphological and molecular techniques. Place and Duration of Study: Department of Plant Pathology, TNAU, Coimbatore, Tamil Nadu from April 2018 to November 2019. Methodology: Endophytic fungal isolates were isolated from different parts of cocoa using Petrini method. Isolated endophytic fungal strains were grown in Malt extract broth and total genomic DNA was isolated and amplified using universal primers ITS1F and ITS4R. Amplified rDNA was visualized and documented. Results: Morphological and molecular characterization of six endophytic fungi revealed that they are from four different taxa viz., Lasiodiplodia pseudotheobromae PAK-7, Arthrinium rasikravindrae P11, Arthrinium rasikravindrae P12, Diaporthe sp. Vef-3, Lasiodiplodia theobromae TN-R-3,  Colletotrichum sp. TN-9-2 belonging to four different families viz., Botryosphaeriaceae, Apiosporaceae, Diaporthaceae and Glomorellaceae under Phylum Ascomycota. Conclusion:  The present study indicates the distribution and diversity of fungal endophytes in different plant parts of the cocoa tree in south India.

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