scholarly journals Persistence of ectomycorrhizas by Thelephora terrestris on outplanted Scots pine seedlings

2013 ◽  
Vol 41 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Dorota Hilszczańska ◽  
Zbigniew Sierota
Keyword(s):  
Scots Pine ◽  
Nitrogen Availability ◽  
Agricultural Land ◽  
Rflp Analysis ◽  
Mycorrhizal Symbiosis ◽  
Thelephora Terrestris ◽  
Pine Seedlings ◽  
Pcr Rflp ◽  
Conifer Trees

<em>Thelephora terrestris</em> (Erhr.) Fr. is a very common ectomycorrhizal symbiont (ECM) in conifer trees, however the role of this ubiquitous fungus in nurseries and Scots pine plantations is still unknown. It is described as tolerant of high nitrogen availability and therefore was taken into consideration as an important ECM partner of seedlings, particularly after replanting on post agricultural land. In laboratory the seedlings of Scots pine (<em>Pinus sylvestris</em> L.) were inoculated with <em>T. terrestris</em> (Tt/IBL/2) or not inoculated (control) and grown in containers in two different regimes of nitrogen fertilization (4g N x kg<sup>-1</sup> and 6 g N x kg<sup>-1</sup>). Next year these seedlings were outplanted in post agricultural land and 6 months later, the number and identity of some mycorrhizas were studied. It was found, that mycorrhizal abundance was higher in the inoculated treatments than in non-inoculated ones. PCR RFLP analysis confirmed share of two different isolates of <em>Thelephora</em> engaged in mycorrhizal symbiosis. Part of mycorrhizas had the same pattern of RFLP as the isolate used to inoculation. Similar results were obtained in second year of experimental study in the field what confirmed the persistence of artificially introduced <em>T. terrestris</em> in post agricultural soil as an important component of the ECM community.

scholarly journals THE PREVALENCE OF PAI-1 4G/5G POLYMORPHISM IN WOMEN WITH FETAL LOSS – FIRST DATA FOR A SERBIAN POPULATION / UČESTALOST POLIMORFIZMA PAI-1 4G/5G KOD ŽENA SA SPONTANIM POBAČAJEM - PRVI PODACI ZA SRPSKU POPULACIJU

2013 ◽  
Vol 33 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Valentina Đorđević ◽  
Maja Gvozdenov ◽  
Iva Pruner ◽  
Mirjana Kovač ◽  
Branko Tomić ◽  
...  
Keyword(s):  
Fetal Loss ◽  
Rflp Analysis ◽  
Plasminogen Activator Inhibitor ◽  
Second Trimester ◽  
Plasminogen Activator Inhibitor 1 ◽  
Over Expression ◽  
Increased Risk ◽  
Pcr Rflp ◽  
Pai 1

Summary Background: Plasminogen activator inhibitor 1 (PAI-1) is an inhibitor of fibrinolysis. The PAI-1 4G/5G polymorphism is associated with elevated plasma levels of PAI-1. Over- expression of PAI-1 and impaired fibrinolysis in homozygous carriers of the 4G/4G PAI polymorphism may lead to abnor- mal placental formation and increased risk of fetal loss (FL). The aim of our study was to determine the frequency of this polymorphism in patients with FL in a Serbian population. Methods: The study was carried out in a group of 203 women (91 controls and 112 women with FL). The presence of PAI-1 4G/5G polymorphism was detected by PCR-RFLP analysis. Results: Slightly increased frequency of the PAI-1 4G/4G genotype was observed in the study group compared to the controls (32.1% vs. 30.8%). The frequency of PAI-1 was highest in women experiencing FL in the second trimester of pregnancy (50%), but this difference was not statistically sig- nificant. Conclusions: Our findings suggest that PAI-1 4G/4G might be a risk factor for FL occurring in the second trimester of pregnancy. Further studies are required in order to determine the role of PAI-1 4G/5G polymorphism in the etiology of FL.

scholarly journals Changes in nitrogen level and mycorrhizal structure of Scots pine seedlings inoculated with Thelephora terrestris

2008 ◽  
Vol 65 (4) ◽  
pp. 409-409 ◽  
Author(s):  
Dorota Hilszczańska ◽  
Monika Małecka ◽  
Zbigniew Sierota
Keyword(s):  
Scots Pine ◽  
Nitrogen Level ◽  
Thelephora Terrestris ◽  
Pine Seedlings

scholarly journals The role of polymorphism of genes associated with cardiovascular system, in the formation lipodystrophy

2015 ◽  
Vol 14 (1) ◽  
pp. 67-73
Author(s):  
O. S. Glotov ◽  
I. V. Polyakova ◽  
T. N. Korolkova ◽  
A. V. Sogomonyan ◽  
Y. I. Sternin ◽  
...  
Keyword(s):  
Cardiovascular System ◽  
Design Methodology ◽  
Essential Role ◽  
Rflp Analysis ◽  
Blood Microcirculation ◽  
Wide Range ◽  
Pcr Rflp ◽  
Formation Method ◽  
Practical Implications

Purpose. The wide range of the factors promoting emergence of GLD and also specificity of racial features of this disease testify to importance of studying of the genetic reasons of hereditary predisposition to development of GLD. Design/methodology/approach. PCR-RFLP analysis of the studied polymorphism of genes associated with the formation method of lipodystrophy, and estimated of genetic testing results. Findings. There is a correlation between GLD and allelic variants of genes (MMP3, ACE, NOS3), whose products are involved in collagen metabolism, and the cardiovascular system by regulating blood microcirculation the violation of which plays the essential role in the development of GLD. Practical implications. These results suggest the use of treatment aimed at solving the problems of the microcirculation is important for the treatment of patients with GLD.

scholarly journals Soluble Fas and the −670 Polymorphism of Fas in Lupus Nephritis

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Juan José Bollain-y-Goytia ◽  
Mariela Arellano-Rodríguez ◽  
Felipe de Jesús Torres-Del-Muro ◽  
Leonel Daza-Benítez ◽  
José Francisco Muñoz-Valle ◽  
...  
Keyword(s):  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Healthy Subjects ◽  
Rflp Analysis ◽  
Allelic Frequency ◽  
Soluble Fas ◽  
Pcr Rflp ◽  
Sfas Level

This study was performed to clarify the role of soluble Fas (sFas) in lupus nephritis (LN) and establish a potential relationship between LN and the −670 polymorphism of Fas in 67 patients with systemic lupus erythematosus (SLE), including a subset of 24 LN patients with proteinuria. Additionally, a group of 54 healthy subjects (HS) was included. The allelic frequency of the −670 polymorphism of Fas was determined using PCR-RFLP analysis, and sFas levels were assessed by ELISA. Additionally, the WT-1 protein level in urine was measured. The Fas receptor was determined in biopsies by immunohistochemistry (IHC) andin situhybridization (FISH) and apoptotic features by TUNEL.Results. The −670 Fas polymorphism showed that the G allele was associated with increased SLE susceptibility, with an odds ratio (OR) of 1.86. The sFas was significantly higher in LN patients with the G/G genotype, and this subgroup exhibited correlations between the sFas level and proteinuria and increased urinary WT-1 levels. LN group shows increased expression of Fas and apoptotic features. In conclusion, our results indicate that the G allele of the −670 polymorphism of Fas is associated with genetic susceptibility in SLE patients with elevated levels of sFas in LN with proteinuria.

Whole-plant frost hardiness of mycorrhizal (Hebeloma sp. or Suillus luteus) and non-mycorrhizal Scots pine seedlings

2018 ◽  
Vol 39 (6) ◽  
pp. 951-960 ◽  
Author(s):  
Anna Korhonen ◽  
Tarja Lehto ◽  
Jaakko Heinonen ◽  
Tapani Repo
Keyword(s):  
Scots Pine ◽  
Reverse Flow ◽  
Visual Assessment ◽  
Frost Hardiness ◽  
Mycorrhizal Symbiosis ◽  
Nitrogen And Phosphorus ◽  
Suillus Luteus ◽  
Whole Plant ◽  
Hardening Treatment

Abstract Ectomycorrhizal trees are common in the cold regions of the world, yet the role of the mycorrhizal symbiosis in plant cold tolerance is poorly known. Moreover, the standard methods for testing plant frost hardiness may not be adequate for roots and mycorrhizas. The aims of this study were to compare the frost hardiness of mycorrhizal and non-mycorrhizal Scots pine (Pinus sylvestris L.) seedlings and to test the use of reverse-flow root hydraulic conductance (Kr) measurement for root frost hardiness determination. Mycorrhizal (Hebeloma sp. or Suillus luteus) and non-mycorrhizal seedlings were grown in controlled-environment chambers for 13 weeks. After this, half of the plants were allotted to a non-hardening treatment (long day and high temperature, same as during the preceding growing season) and the other half to a hardening (short day and low temperature) ‘autumn’ treatment for 4 weeks. The intact seedlings were exposed to whole-plant freezing tests and the needle frost hardiness was measured by relative electrolyte leakage (REL) method. The seedlings were grown for three more weeks for visual damage assessment and Kr measurements using a high-pressure flow meter (HPFM). Mycorrhizas did not affect the frost hardiness of seedlings in either hardening treatment. The effect of the hardening treatment on frost hardiness was shown by REL and visual assessment of the aboveground parts as well as Kr of roots. Non-mycorrhizal plants were larger than mycorrhizal ones while nitrogen and phosphorus contents (per unit dry mass) were similar in all mycorrhiza treatments. In plants with no frost exposure, the non-mycorrhizal treatment had higher Kr. There was no mycorrhizal effect on plant frost hardiness when nutritional effects were excluded. Further studies are needed on the role of mycorrhizas especially in the recovery of growth and nutrient uptake in cold soils in the spring. The HPFM is useful novel method for assessment of root damage.

The role of low-level ozone exposure and mycorrhizas in chemical quality and insect herbivore performance on Scots pine seedlings

2000 ◽  
Vol 6 (1) ◽  
pp. 111-121 ◽  
Author(s):  
Anne-Marja Manninen ◽  
Toini Holopainen ◽  
Päivi Lyytikäinen-Saarenmaa ◽  
Jarmo K. Holopainen
Keyword(s):  
Scots Pine ◽  
Ozone Exposure ◽  
Insect Herbivore ◽  
Chemical Quality ◽  
Herbivore Performance ◽  
Low Level ◽  
Pine Seedlings

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp
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