scholarly journals Comparison of enzymatic activity of selected yeast-like fungi isolated from lakes and astatic reservoirs

2014 ◽  
Vol 33 (1) ◽  
pp. 37-42
Author(s):  
Maria Dynowska ◽  
Anna Biedunkiewicz
Keyword(s):  
Candida Albicans ◽  
Chemical Composition ◽  
Enzymatic Activity ◽  
Morphological Differentiation ◽  
Water Reservoirs ◽  
Physiological Differences ◽  
Trichosporon Beigelii

The stains of <i>Candida albicans</i> and <i>Trichosporon beigelii</i> isolated from astatic reservoir show a higher enzymatic activity than the strains isolated from lake. The results obtained confirm earlier suspicions that the morphological differentiation of micro-fungi living in different water reservoirs is generally accompanied by physiological differences, which arc, to the largest extent, reflections of the chemical composition of the environment.

scholarly journals Interactions between Terpinen-4-ol and Nystatin on biofilm of Candida albicans and Candida tropicalis

2018 ◽  
Vol 29 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Caroline Coradi Tonon ◽  
Renata Serignoli Francisconi ◽  
Ester Alves Ferreira Bordini ◽  
Patrícia Milagros Maquera Huacho ◽  
Janaína de Cássia Orlandi Sardi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Enzymatic Activity ◽  
Candida Tropicalis ◽  
Microtiter Plate ◽  
Synergistic Activity ◽  
Oral Keratinocytes ◽  
Mixed Species ◽  
Colony Forming Units ◽  
Anti Fungal ◽  
Oral Cells

Abstract The aim of this study was to evaluate the antifungal activity of Terpinen-4-ol associated with nystatin, on single and mixed species biofilms formed by Candida albicans and Candida tropicalis, as well as the effect of terpinen-4-ol on adhesion in oral cells and the enzymatic activity. The minimum inhibitory concentrations and minimum fungicide concentrations of terpinen-4-ol and nystatin on Candida albicans and Candida tropicalis were determined using the microdilution broth method, along with their synergistic activity (“checkerboard” method). Single and mixed species biofilms were prepared using the static microtiter plate model and quantified by colony forming units (CFU/mL). The effect of Terpinen-4-ol in adhesion of Candida albicans and Candida tropicalis in coculture with oral keratinocytes (NOK Si) was evaluated, as well as the enzymatic activity by measuring the size of the precipitation zone, after the growth agar to phospholipase, protease and hemolysin. Terpinen-4-ol (4.53 mg mL-1) and nystatin (0.008 mg mL-1) were able to inhibit biofilms growth, and a synergistic antifungal effect was showed with the drug association, reducing the inhibitory concentration of nystatin up to 8 times in single biofilm of Candida albicans, and 2 times in mixed species biofilm. A small decrease in the adhesion of Candida tropicalis in NOK Si cells was showed after treatment with terpinen-4-ol, and nystatin had a greater effect for both species. For enzymatic activity, the drugs showed no action. The effect potentiated by the combination of terpinen-4-ol and nystatin and the reduction of adhesion provide evidence of its potential as an anti-fungal agent.

scholarly journals Chemical Composition and Preliminary Antimicrobial Activity of the Hydroxylated Sesquiterpenes in the Essential Oil from Piper barbatum Kunth Leaves

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 211 ◽  
Author(s):  
Paco Noriega ◽  
José Ballesteros ◽  
Alejandra De la Cruz ◽  
Tatiana Veloz
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Forest Ecosystem ◽  
Raw Materials ◽  
The Andes ◽  
Ancestral Knowledge ◽  
Antifungal Potential

This study evaluates the antimicrobial and antifungal potential of the essential oil extracted from a species located in the Andes of Ecuador, Piper barbatum Kunth, known as “cordoncillo” or “allupa”, used by the Quichua people as an antibacterial plant for washing female genitalia in cases of infection. The most abundant molecules in the essential oil were: α- phellandrene (43.16%), limonene (7.04%); some oxygenated sesquiterpenes such as: trans-sesquisabinene hydrate (8.23%), elemol (7.21%) and others. The evaluation of antimicrobial activity showed activity in all the strains analyzed; however, those in which MIC values are considered to be very strong (less than 500 µg/mL) are: Staphylococcus aureus 264 µg/mL, Streptococcus mutans 132 µg/mL, Candida albicans 132 µg/mL and Candida tropicalis 264 µg/mL. Antimicrobial bioautography defines which molecules are responsible for the activity; thus, it was possible to establish the chromatographic regions of = 0.02 and Rf = 0.04, as those with active molecules. It was established that 4 hydroxylated sesquiterpene molecules are involved: elemol (7.21%), trans-sesquisabinene hydrate (8.23%), β–eudesmol (3.49%) and 10-epi-γ-eudesmol (1.07%); the last two being the most active. The aim of this manuscript is to analyze both the ancestral knowledge of the Quichua people of Ecuador, and the chemical-biodiversity of the Andean forest ecosystem, in order to provide new raw materials of pharmaceutical interest.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oil from Chaerophyllum Aureum L. (Apiaceae)

2009 ◽  
Vol 4 (1) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Branislava Lakušić ◽  
Violeta Slavkovska ◽  
Milica Pavlović ◽  
Marina Milenković ◽  
Jelena Antić Stanković ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Micrococcus Luteus ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aerial Parts

The essential oils of the aerial parts and fruits of Chaerophyllum aureum L., collected from two mountains in Serbia, were analyzed by GC and GC/MS. Sabinene (18.5-31.6%), p-cymene (7.9-25.4%) and limonene (1.9-10.9%) were characterized as the main constituents. The oils were tested against six bacterial strains and one strain of yeast, Candida albicans. The highest antimicrobial activity was observed against the Gram-positive bacteria Staphylococcus aureus, S. epidermidis and Micrococcus luteus, while of the Gram-negative strains, Escherichia coli was the most sensitive.

scholarly journals Molecular Biological Approach to Morphological Differentiation in Candida albicans.

1997 ◽  
Vol 38 (4) ◽  
pp. 285-290
Author(s):  
Tamaki Cho ◽  
Hisako Hamatake ◽  
Takeshi Hirota ◽  
Hidenori Kaminishi ◽  
Yoshisato Hagihara ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Morphological Differentiation ◽  
Biological Approach ◽  
Molecular Biological Approach

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Chemical Composition, Antifungal and Antibacterial Activity of the Essential Oil of Chamaecyparis Nootkatensis from Spain

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Jesús Palá-Paúl ◽  
Jaime Usano-Alemany ◽  
Elena Granda ◽  
Ana-Cristina Soria
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Micrococcus Luteus ◽  
Enterobacter Aerogenes ◽  
Chamaecyparis Nootkatensis ◽  
Antibacterial And Antifungal Activities ◽  
Total Oil

The chemical composition of the oil of Chamaecyparis nootkatensis (D. Don) Spach. has been analysed by gas chromatography (GC) and gas chromatography coupled to mass spectrometry (GCMS). A total of 34 compounds were identified representing more than 90% of the total oil. The oil was richer in monoterpenes than in sesquiterpenes, the major constituents being limonene (53.2%), δ-3-carene (21.0%) and α-pinene (12.2%). The antibacterial and antifungal activities of the oil were also tested against Candida albicans, Bacillus subtilis, Enterobacter aerogenes, Enterococcus faecalis, Escherichia coli, Micrococcus luteus, Pseudomonas aeruginosa, Salmonella sp. and Serratia marcescens. Only two of these, Bacillus subtilis and Candida albicans, were sensitive to the treatment, inhibition zones of 11 and 14 mm diameter being obtained, respectively. As far as we know, this is the first report of the antifungal and antibacterial activity of this species.

scholarly journals Essential Oil Composition and Antimicrobial Activity of Aerial Parts and Ripe Fruits of Echinophora spinosa (Apiaceae) from Italy

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800
Author(s):  
Daniele Fraternale ◽  
Salvatore Genovese ◽  
Donata Ricci
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Intestinal Microflora ◽  
Central Italy ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Aerial Parts ◽  
Eubacterium Limosum

The chemical composition and antimicrobial activity of the essential oils obtained from the flowering aerial parts and ripe fruits of Echinophora spinosa L. (Apiaceae) from central Italy were analyzed by GC/MS. The major constituents of the oil from the aerial parts were β-phellandrene (34.7%), myristicin (16.5%), δ3-carene (12.6%), α-pinene (6.7%) and α-phellandrene (6.2%), and of the oil from the ripe fruits p-cymene (50.2%), myristicin (15.3%), α-pinene (15.1%) and α-phellandrene (8.1%). The two oils showed good antimicrobial activity against Clostridium difficile, C perfringens, Enterococcus faecalis, Eubacterium limosum, Peptostreptococcus anaerobius and Candida albicans with MIC values respectively of 0.25, 0.25, 0.25, 0.25, 2.25, and 0.50%, v/v, and 0.13, 0.13, 0.13, 0.13, 2.25, 0.50%, v/v, for aerial parts and ripe fruits respectively. A less significant antimicrobial activity against bifidobacteria and lactobacilli, very important in the intestinal microflora, was also detected, with MIC values higher than 4.0%, v/v.

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