scholarly journals Micromorphology and anatomy of flowers and nectaries of Saxifraga stolonifera L.

2014 ◽  
Vol 67 (4) ◽  
pp. 3-12 ◽  
Author(s):  
Agata Konarska
Keyword(s):  
Electron Microscopy ◽  
Glandular Trichomes ◽  
Secretory Cells ◽  
Abaxial Surface ◽  
Bright Field ◽  
Vascular Elements ◽  
Yellow Orange ◽  
Modified Stomata ◽  
Micromorphology And Anatomy ◽  
Scanning Electron

Saxifrages are plants commonly found in all continents. Many of them are adapted to flowering and reproduction under mountainous and rocky conditions. They are pollinated by various groups of insects and have intrastaminal nectaries. The morpho-anatomy of the flowers and nectaries of <em>Saxifraga stolonifera</em> L. was examined using bright-field light and stereoscopic microscopy as well as scanning electron microscopy. The abaxial surface of the sepals has multicellular glandular trichomes containing anthocyanins in the base cells and polyphenols in the secretory cells of the head, whereas visual attractants in the form of color spots are found on the petals. The nectary gland is located at the apex of the ovary and forms a yellow-orange fleshy half-ring. Nectar is secreted through numerous modified stomata. The glandular parenchyma does not have vascular elements. Moreover, orange-brown polyphenols were observed in the nectary cells.

scholarly journals Micromorphology and anatomy of three Symphytum (Boraginaceae) taxa from Turkey

1970 ◽  
Vol 36 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Oznur Ergen Akcin ◽  
Hilal Baki
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Key Words ◽  
Leaf Surface ◽  
Glandular Trichomes ◽  
Epidermal Cells ◽  
Lower Epidermis ◽  
Micromorphology And Anatomy ◽  
Scanning Electron

Symphytum asperum Lepechin, S. ibericum Steven and S. sylvaticum Boiss. were examined morphologically, micromorphologically and anatomically. Scanning electron microscopy was used to examine leaf surface and trichomes of these species. These species had bifacial and hypostomatous leaf types. Epidermal cells of leaves were usually polygonal or irregular in form. The pattern of anticlinical cells may vary in different species and between the upper and lower epidermis of the same species. Stomata are anisocytic and anomocytic in three species. Stomata index is 27.5 for S. sylvaticum, 24.65 for S. ibericum and 21.86 for S. asperum glandular trichomes are capitate in forms and more dense on the lower epidermis than upper epidermis. Eglandular trichomes are simple, short or long, unicellular or multicellular and thin or thick. Key words: Micromorphology, Anatomy, Symphytum DOI = 10.3329/bjb.v36i2.1496 Bangladesh J. Bot. 36(2): 93-103, 2007 (December)   

scholarly journals Morphological differentiation and distribution of non-glandular and glandular trichomes on Dracocephalum moldavicum L. shoots

2012 ◽  
Vol 63 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Elżbieta Weryszko-Chmielewska ◽  
Marta Dmitruk
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Morphological Differentiation ◽  
Structural Variations ◽  
Abaxial Surface ◽  
The Rich ◽  
Trichome Types ◽  
Scanning Electron

The structure, micromorphology and distribution of trichomes on <i>Dracocephalum moldavicum</i> L. shots were investigated using light and scanning electron microscopy (SEM). There were distinguished 3 types of non-glandular trichomes, 3 types of glandular trichomes and papillae of the osmophore in the epidermis of the corolla. The highest density of non-glandular and glandular trichomes was found on the abaxial surface of the calyx, on bracts and in the upper part of the stem. Structural variations in the head and stalk of long glandular trichomes were demonstrated. The dimensions of particular types of trichomes are given in the present paper. Differences in the structure of the trichomes of this species are presented, compared to literature data. The micromorphology of particular trichome types is documented in numerous photographs showing the rich ornamentation of the cuticle of non-glandular trichomes.

Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species

2017 ◽  
Vol 23 (3) ◽  
pp. 679-686 ◽  
Author(s):  
Milan Gavrilović ◽  
Suzana Erić ◽  
Petar D. Marin ◽  
Núria Garcia-Jacas ◽  
Alfonso Susanna ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Energy Dispersive ◽  
Spectrometric Analysis ◽  
Eds Analysis ◽  
Taxonomic Studies ◽  
First Time ◽  
Tetragonal Bipyramid ◽  
Scanning Electron

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.

Coelomomyces canadense stat. et comb. nov. (Blastocladiales: Coelomomycetaceae) from Psectrocladius sp. G (Diptera: Chironomidae)

1978 ◽  
Vol 56 (18) ◽  
pp. 2303-2306 ◽  
Author(s):  
Richard A. Nolan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phase Contrast ◽  
Field Phase ◽  
Species Status ◽  
Bright Field ◽  
Latin Diagnosis ◽  
Taxonomic Criterion ◽  
Sporangial Wall ◽  
Scanning Electron

Resistant sporangia of Coelomomyces chironomi var. canadense Weiser and McCauley were examined by bright-field, phase-contrast, and scanning electron microscopy (SEM). The use of SEM facilitated the observation of previously undescribed complex furrows in the sporangial wall. The taxonomic criterion for varietal status is discussed, and the variety is elevated to species status. Coelomomyces canadense (Weiser and McCauley) Nolan stat. et comb. nov. is described with an emended Latin diagnosis.

scholarly journals Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Systematic Significance ◽  
Trichome Types ◽  
First Time ◽  
Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

Glandular Trichomes and the Yolatiles Obtained by Steam Distillation of Quercus robur Leaves

1993 ◽  
Vol 48 (9-10) ◽  
pp. 736-744 ◽  
Author(s):  
Ralf Engel ◽  
Paul-Gerhard Gülz ◽  
Thorsten Herrmann ◽  
Adolf Nahrstedt
Keyword(s):  
Electron Microscopy ◽  
Quercus Robur ◽  
Mass Spectra ◽  
Steam Distillation ◽  
Glandular Trichomes ◽  
Retention Indices ◽  
Mass Spectral Data ◽  
Mass Spectral ◽  
Kováts Indices ◽  
Scanning Electron

Glandular trichomes in form of long stretched tubes are present on the lower leaf side of Quercus robur as shown by scanning electron microscopy. The glands contain an essential oil, which was isolated by steam distillation together with volatile waxy components of the leaves in an amount of 0.025% of fresh leaves. The product of steam distillation was analyzed by GC-MS. Identification of com pounds is based on comparison of their mass spectral data with those of authentic samples in combination with retention indices and MS data using the SeKoMS (Search Kovats Indices and Mass Spectra) Library. Altogether 184 components of the product of steam distillation were separated, 155 of which could be identified, another 7 were tentatively assigned. Three groups of substances according to their chemical composition are found: hexenyl derivatives and some acetals (32%); terpenes including monoterpenes (4% ), sesquiterpenes and diterpenes (21%); and alkane derivatives (35%). The residual 8% consist of benzyl alcohol, com pounds which stem from the degradation of carotenes, and miscellaneous constituents

scholarly journals Atractis thapari (Nematoda, Atractidae) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí, Brazil

2018 ◽  
Vol 27 (2) ◽  
pp. 146-153
Author(s):  
Anangela Ravena da Silva Leal ◽  
Simone Mousinho Freire ◽  
Marcelo Knoff ◽  
Delir Corrêa Gomes ◽  
Jeannie Nascimento dos Santos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Neotropical Region ◽  
Health Conditions ◽  
Taxonomic Identification ◽  
Bright Field ◽  
New Host ◽  
Male And Female ◽  
Mean Intensity ◽  
Scanning Electron

Abstract Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.

An Inexpensive Approach for Bright-Field and Dark-Field Imaging by Scanning Transmission Electron Microscopy in Scanning Electron Microscopy

2014 ◽  
Vol 20 (1) ◽  
pp. 124-132 ◽  
Author(s):  
Binay Patel ◽  
Masashi Watanabe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Lattice Spacing ◽  
Dark Field ◽  
Bright Field ◽  
Specimen Holder ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Scanning Electron

AbstractScanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) is a convenient technique for soft materials characterization. Various specimen-holder geometries and detector arrangements have been used for bright-field (BF) STEM-in-SEM imaging. In this study, to further the characterization potential of STEM-IN-SEM, a new specimen holder has been developed to facilitate direct detection of BF signals and indirect detection of dark-field (DF) signals without the need for substantial instrument modification. DF imaging is conducted with the use of a gold (Au)-coated copper (Cu) plate attached to the specimen holder which directs highly scattered transmitted electrons to an off-axis yttrium-aluminum-garnet (YAG) detector. A hole in the copper plate allows for BF imaging with a transmission electron (TE) detector. The inclusion of an Au-coated Cu plate enhanced DF signal intensity. Experiments validating the acquisition of true DF signals revealed that atomic number (Z) contrast may be achieved for materials with large lattice spacing. However, materials with small lattice spacing still exhibit diffraction contrast effects in this approach. The calculated theoretical fine probe size is 1.8 nm. At 30 kV, in this indirect approach, DF spatial resolution is limited to 3.2 nm as confirmed experimentally.

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