scholarly journals Influence of Bacillus polymyxa on the growth and development of Fusarium oxysporum f. sp. tulipae

2013 ◽  
Vol 52 (1-2) ◽  
pp. 5-10
Author(s):  
Alicja Saniewska
Keyword(s):  
Fusarium Oxysporum ◽  
Spore Germination ◽  
Growth And Development ◽  
Antagonistic Effect ◽  
Potato Dextrose Agar ◽  
Bacillus Polymyxa ◽  
Dual Cultures ◽  
Tulip Bulbs

Antagonistic effect of Bacillus polymyxa, strain S13, toward Fusarium oxysporum f. sp. tulipae was evaluated iii vitro and in vivo. The growth of the pathogen was greatly inhibited in dual cultures with Bacillus polymyxa on potato dextrose agar. Suspension of B. polymyxa and its filtrate substantially inhibited spore germination and development of Fusarium oxysporuum f. sp. tulipae on tulip bulbs.

scholarly journals The role of gum induced by Fusarium oxysporum Schlecht. Snyd. et Hans. f.sp. tulipae Apt. in tulip bulbs on growth and development of the pathogen

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 432-435
Author(s):  
A. Saniewska
Keyword(s):  
Fusarium Oxysporum ◽  
Growth And Development ◽  
Mycelium Growth ◽  
Tulip Bulbs ◽  
Bacto Agar

It was showed that gums induced by Fusarium oxysporum f.sp. tulipae in tulip bulbs applied to mineral Czapek-Dox Broth with Bacto Agar (CzDA) medium, containing sucrose substantially stimulated abundant growth of mycelium and sporulation of the pathogen. Addition of arabinose, xylose and their mixture, sugars occurring in tulip gum polysaccharide, to CzDA medium caused that mycelium was very sparse and sporulation was only slightly stimulated by arabinose. Mycelium growth on mineral CzDA medium without sucrose, was poor and sparse but addition of gum to the medium caused formation of abundant mycelium and increased sporulation of the pathogen. It is possible that polysaccharide of tulip gum may act mainly as elicitor and partially as substrate in regulation of mycelium growth and sporulation of Fusarium oxysporum f.sp. tulipae.

Pengembangan Teknologi Pengendalian Penyakit Layu Tomat (Fusarium oxysporum f. sp. lycopersici) yang Ramah Lingkungan dengan Menggunakan Mikroorganisme Saprofit

Agrotek ◽  
2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Derek Kornelis Erari ◽  
Eko Agus Martanto
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Growth And Development ◽  
Wilt Disease ◽  
Common Disease ◽  
Microbial Antagonism ◽  
The Earth ◽  
Tomato Wilt

Fusarium wilt disease is a common disease for tomatoes plant which damages plant although it is intensively cultivated. Biological control by microbial antagonism is environmentally friendly compare to the use of fungicide. This research was aimed to study the capability of Bacillus subtilis, Pseudomonas fluorescens, Gliocladium fimbriatum and Trichoderma viridae that are locally specific in controll Fusarium oxysporum f.sp. lycopersici� cause tomato wilt disease. Evaluation of F.o. f.sp. lycopersici� antagonism capability was done by in vitro and in vivo.� In vitro antagonism treatment used the ouble plating treatment on Potato Dextose Agar.� In vivo antagonism treatment was done by gave antagonism agent and F.o. f.sp. lycopersici� together to the earth medium. The result of the study revealed that (1) B. subtilis, G. fimbriatum and T. viridae can pressure the growth and development �F.o. f.sp. lycopersici which cause tomato wilt disease both in vitro and in vivo, (2) In vitro the average of the prevented pathogen growth was as follow: B. subtilis (55.52%), G. fimbriatum (36.77%) and� T. viridae (35.87%), (3) In vivo treatment by the earth medium was not significantly different, and (4) In vivo the ability of prevent disease intensity was as follow : B. subtilis (62.35%), G. fimbriatum (49.95%) and� T. viridae (49.95%).

scholarly journals The effect of D,L-β-aminobutyric acid on the growth and development of Fusarium oxysporum f. sp. tulipae (Apt.)

2012 ◽  
Vol 60 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Anna Jarecka ◽  
Alicja Saniewska
Keyword(s):  
Fusarium Oxysporum ◽  
Root System ◽  
Growth And Development ◽  
Linear Growth ◽  
Aminobutyric Acid ◽  
Fresh Weight ◽  
Mycelium Growth ◽  
Disease Symptoms ◽  
Tulip Bulbs ◽  
Inducing Resistance

The effect of D,L-<i>β</i>-aminobutyric acid (BABA) on the growth and development of the root system and the development of fusariosis on tulip bulbs cv. Apeldoorn infected by <i>Fusarium oxysporum</i> f. sp. <i>tulipae</i> (F.ox.t. 218) was studied. The length and fresh weight of roots, the development of fusariosis on bulbs and the linear growth of mycelium of F.ox.t. 218 on PDA medium were measured. Preventively used BABA at a concentration of 100, 250 and 300 µg·cm<sup>-3</sup> for soaking uncooled and cooled tulip bulbs greatly inhibited the development of fusariosis on the root system; the length and fresh weight of roots were similar to those of the bulbs not inoculated with F.ox.t. 218. At a concentration of 100 µg·cm<sup>-3</sup>;, BABA used for soaking bulbs limited the development of fusariosis on scales in about 50% and the concentration of 200 µg·cm<sup>-3</sup> totally inhibited the disease symptoms induced by F.ox.t. 218. At a concentration of 100 - 1000 µg·cm<sup>-3</sup>, BABA did not inhibit the mycelium growth of F.ox.t. 17 and F.xo.t. 218 on PDA medium. This study suggests that BABA protects tulip roots and bulb scales against <i>F. oxysporum</i> f. sp. <i>tulipae</i> by inducing resistance in these organs and has no direct influence on the pathogen.

scholarly journals The effect of Medicago arabica, M. hybrida and M. sativa saponins on the growth and development of Fusarium oxysporum Schlecht f. sp. tulipae apt.

2012 ◽  
Vol 61 (2) ◽  
pp. 147-155 ◽  
Author(s):  
Anna Jarecka ◽  
Alicja Saniewska ◽  
Zbigniew Biały ◽  
Marian Jurzysta
Keyword(s):  
Fusarium Oxysporum ◽  
Growth And Development ◽  
Inhibitory Effect ◽  
Mycelium Growth ◽  
Medicagenic Acid ◽  
Colony Forming Units ◽  
Tulip Bulbs

In the present work it was shown that total saponins originated from <i>M. hybrida</i> and <i>M. sativa</i> substantially limited mycelium growth of <i>F. oxysporum</i> f. sp. <i>tulipae</i> and symptoms of fusariosis on tulip bulbs. Out of 15 individual tested saponins originated from <i>M. arabica</i>, <i>M. hybrida</i> and <i>M. sativa</i>, four compounds: 3-<i>O</i>-[β-D-glucopyranosyl (1→2)α-L-arabinopyranosyl] hederagenin, hederagenin 3-<i>O</i>-β-D-glucopyranoside, medicagenic acid, medicagenic acid 3-<i>O</i>-β-D-glucopyranoside had the strongest inhibitory effect on mycelium growth of <i>Fusarium oxysporum</i> f. sp. <i>tulipae</i> on PDA medium. The total saponins from <i>M. arabica</i>, <i>M. hybrida</i> and <i>M. sativa</i> inhibited the number of colony forming units of <i>Fusarium oxysporum</i> f. sp. <i>tulipae</i> in artificially infested substrate. The use of saponins originated from <i>Medicago</i> as a fungicide is suggested.

In vivo Experiments of Natural Products Protection of Antagonistic Effects of Lead on Iron

2018 ◽  
Vol 68 (12) ◽  
pp. 2747-2751
Author(s):  
Marioara Nicula ◽  
Nicolae Pacala ◽  
Lavinia Stef ◽  
Ioan Pet ◽  
Dorel Dronca ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Iron Homeostasis ◽  
Iron Concentration ◽  
Antagonistic Effect ◽  
Tissue Samples ◽  
Antagonistic Effects ◽  
In Vivo Experiments ◽  
Living Organisms ◽  
Toxic Potential

Living organisms take nutrients from the environment, and together with them, substances with toxic potential � such as heavy metals. Lead is one common metal pollutant especially in aquatic environment, from where the fish can be intoxicated very easily. Bioavailability, distribution, toxic action, synergistic and antagonistic effects are characteristics which can alter the fish health. Our experimental study followed the effects of lead overload in water on iron distribution, in different tissues sample Carassius gibelio Bloch fish. We performed the experiment in four different fish groups: control C; lead � Pb (administration of lead in water 0.075mg/mL of water, as Pb(NO3)2 x � H2O); lead (the same dose) and 2% of freeze-dry garlic incorporated into fishes� food � Pb+garlic; lead (the same dose) and 2% chlorella incorporated into fishes� food � Pb+chlorella, for 21 consecutive days. The iron concentration was analysed with AAS (Atomic Absorption Spectroscopy) from gills, muscle, skin (and scales), intestine, liver, heart, brain, ovary, testicles, and kidney. The obtained data presented a significantly decrease of iron content in all tested tissue samples that demonstrated, alteration of iron homeostasis, explained by a strong antagonistic effect of lead on iron. Our experiment showed that biologic active principles from garlic and chlorella act like natural protectors, and potentiate the iron deficiency even in the case of lead overload in aquatic environment, for fish.

scholarly journals Growth hormone signaling and action in obese versus lean human subjects

2019 ◽  
Vol 316 (2) ◽  
pp. E333-E344 ◽  
Author(s):  
Morten Lyng Høgild ◽  
Ann Mosegaard Bak ◽  
Steen Bønløkke Pedersen ◽  
Jørgen Rungby ◽  
Jan Frystyk ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Growth Hormone ◽  
Human Subjects ◽  
Glucose Production ◽  
Relative Increase ◽  
Antagonistic Effect ◽  
Endogenous Glucose Production ◽  
Metabolic Adaptation ◽  
Igf I

Growth hormone (GH) levels are blunted in obesity, but it is not known whether this relates to altered GH sensitivity and whether this influences the metabolic adaptation to fasting. Therefore, we investigated the effect of obesity on GH signal transduction and fasting-induced changes in GH action. Nine obese (BMI 35.7 kg/m2) and nine lean (BMI 21.5 kg/m2) men were studied in a randomized crossover design with 1) an intravenous GH bolus, 2) an intravenous saline bolus, and 3) 72 h of fasting. Insulin sensitivity (hyperinsulinemic, euglycemic clamp) and substrate metabolism (glucose tracer and indirect calorimetry) were measured in studies 1 and 2. In vivo GH signaling was assessed in muscle and fat biopsies. GH pharmacokinetics did not differ between obese and lean subjects, but endogenous GH levels were reduced in obesity. GH signaling (STAT5b phosphorylation and CISH mRNA transcription), and GH action (induction of lipolysis and peripheral insulin resistance) were similar in the two groups, but a GH-induced insulin antagonistic effect on endogenous glucose production only occurred in the obese. Fasting-induced IGF-I reduction was completely abrogated in obese subjects despite a comparable relative increase in GH levels (ΔIGF-I: lean, −66 ± 10 vs. obese, 27 ± 16 µg/l; P < 0.01; ΔGH: lean, 647 ± 280 vs. obese, 544 ± 220%; P = 0.76]. We conclude that 1) GH signaling is normal in obesity, 2) in the obese state, the preservation of IGF-I with fasting and the augmented GH-induced central insulin resistance indicate increased hepatic GH sensitivity, 3) blunted GH levels in obesity may protect against insulin resistance without compromising IGF-I status.

scholarly journals Biocontrol of Sclerotinia sclerotiorum and white mold of soybean using saprobic fungi from semi-arid areas of Northeastern Brazil

2015 ◽  
Vol 41 (4) ◽  
pp. 251-255 ◽  
Author(s):  
Daiane Cristina Martins Barros ◽  
Inês Cristina de Batista Fonseca ◽  
Maria Isabel Balbi-Peña ◽  
Sérgio Florentino Pascholati ◽  
Douglas Casaroto Peitl
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Culture Medium ◽  
Antagonistic Effect ◽  
Northeastern Brazil ◽  
White Mold ◽  
Lesion Length ◽  
Fungal Culture ◽  
Saprobic Fungi ◽  
Previously Treated

ABSTRACTThe incidence and the levels of yield loss caused by the white mold of soybean (caused by the fungus Sclerotinia sclerotiorum) have increased in areas of higher altitude at Cerrado and Southern Brazil, causing yield losses of up to 60%. The aim of this study was to select saprobic fungi with the potential to control the white mold of soybean. First, in vitroantagonism screening was carried out to test eight saprobic fungi against S. sclerotiorum. Assessment of S. sclerotiorum mycelial growth was done at four and seven days after its placement on the culture medium. The isolate showing greatest antagonistic effect in all tests/assessments was Myrothecium sp. An in vivo experiment was conducted in a greenhouse and growth chamber, where plants previously treated with eight saprobic fungi were artificially inoculated with S. sclerotiorum. The fungal culture medium (potato-dextrose) and the commercial resistance inducer acibenzolar-S-methyl were used as controls. In the in vivotests, severity of the white mold was assessed at 8, 14 and 21 days after inoculation. The highest reduction percentage in the lesion length was observed for the treatment with Myrothecium sp. (70%), which has the greater potential to be used as biocontrol agent of soybean under the conditions of this experiment.

scholarly journals Physiological Implications of Hydrogen Sulfide in Plants: Pleasant Exploration behind Its Unpleasant Odour

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Zhuping Jin ◽  
Yanxi Pei
Keyword(s):  
Hydrogen Sulfide ◽  
Growth And Development ◽  
Defense Responses ◽  
Plant Responses ◽  
Plant Growth And Development ◽  
Systematic Classification ◽  
Physiological Processes ◽  
Overwhelming Evidence

Recently, overwhelming evidence has proven that hydrogen sulfide (H2S), which was identified as a gasotransmitter in animals, plays important roles in diverse physiological processes in plants as well. With the discovery and systematic classification of the enzymes producing H2Sin vivo, a better understanding of the mechanisms by which H2S influences plant responses to various stimuli was reached. There are many functions of H2S, including the modulation of defense responses and plant growth and development, as well as the regulation of senescence and maturation. Additionally, mounting evidence indicates that H2S signaling interacts with plant hormones, hydrogen peroxide, nitric oxide, carbon monoxide, and other molecules in signaling pathways.

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