scholarly journals Fatty acid and sterol contents during methyl jasmonate-induced leaf abscission in Kalanchoe blossfeldiana

2013 ◽  
Vol 47 (2) ◽  
pp. 83-88
Author(s):  
Marian Saniewski ◽  
Janusz Czapski ◽  
Marcin Horbowicz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Jasmonate ◽  
Leaf Abscission ◽  
Kalanchoe Blossfeldiana ◽  
Kalanchoë Blossfeldiana

It was found previously that methyl jasmonate (JA-Me) induced leaf abscission in <i>Kalanchoe blossfeldiana</i>. In present studies it was showed that JA-Me did not affect or only slightly affected the content of free and bound fatty acids in petioles and blades. ß-Sitosterol, campesterol and ß-amyrin were identified in petioles and blades of <i>K. blossfeldiana</i>; JA-Me decreased the content of campesterol in petioles and increased the content of ß-sitosterol in blades. In blades of plants treated with JA-Me disappearance of olean-12-one was indicated but appearance of 2H-cyclopropa[a]-naphthalen-2-one,l, la, 4, 5, 6, 7, 7a, 7b-octahydro-l, 1, 7, 7a-tetramethyl (aristolone) was documented. The significance of these findings in leaf abscission induced by methyl jasmonate in <i>K. blossfeldiana</i> is discussed.

scholarly journals Activity of cell wall degrading glycanases in methyl jasmonate-induced leaf abscission in Kalanchoe blossfeldiana

2013 ◽  
Vol 48 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Marian Saniewski ◽  
Ewa Gajewska ◽  
Henryk Urbanek
Keyword(s):  
Cell Wall ◽  
Methyl Jasmonate ◽  
Apical Part ◽  
Abscission Zone ◽  
Leaf Abscission ◽  
Cell Wall Polysaccharides ◽  
Kalanchoe Blossfeldiana ◽  
Kalanchoë Blossfeldiana

It was found previously that methyl jasmonate (JA-Me) induced leaf abscission in <em>Kalanchoe blossfeldiana</em>. In present studies it was shown that JA-Me markedly increased the total activities of cellulase, polygalacturonase, pectinase and xylanase in petioles, but did not affect activities of these enzymes in the blades and apical part of shoots of <em>K. blossfeldiana</em>. These results suggest that methyl jasmonate promotes the degradation of cell wall polysaccharides in the abscission zone and in this way induces leaf abscission in <em>Kalanchoe blossfeldiana</em>.

scholarly journals Fatty acid and sterol contents during tulip leaf senescence induced by methyl jasmonate

2013 ◽  
Vol 47 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Marian Saniewski ◽  
Janusz Czapski ◽  
Marcin Horbowicz
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Methyl Jasmonate ◽  
Membrane Fluidity ◽  
Linolenic Acid ◽  
Leaf Senescence ◽  
Bottom Surface ◽  
Induced Changes

It has been shown previously that methyl jasmonate (JA-Me) applied in lanolin paste on the bottom surface of intact tulip leaves causes a rapid and intense its senescence. The aim of this work was to study the effect of JA-Me on free and bound fatty acid and sterol contents during tulip leaf senescence. The main free and bound fatty acids of tulip leaf, in decreasing order of their abundance, were linolenic, linoleic, palmitic, oleic, stearic and myristic acids. Only the content of free linolenic acid decreased after treatment with JA-Me during visible stage of senescence. ß-Sitosterol (highest concentration), campesterol, stigmasterol and cholesterol were identified in tulip leaf. Methyl jasmonate evidently increased the level of ß-sitosterol, campesterol and stigmasterol during induced senescence. It is suggested that the increase in sterol concentrations under the influence of methyl jasmonate induced changes in membrane fluidity and permeability, which may be responsible for senescence.

scholarly journals The Effect Of Some Plant Growth Regulators And Their Combination With Methyl Jasmonate On Anthocyanin Formation In Roots Of Kalanchoe Blossfeldiana

2014 ◽  
Vol 22 (2) ◽  
pp. 31-40 ◽  
Author(s):  
Justyna Góraj ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Marian Saniewski
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Methyl Jasmonate ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Anthocyanin Accumulation ◽  
Kalanchoe Blossfeldiana ◽  
Isolated Roots ◽  
Whole Plants ◽  
Kalanchoë Blossfeldiana

AbstractIn this study, we investigated the effect of plant growth regulators (PGRs) - auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L-1, indole-3-butyric acid (IBA) at 5 mg·L-1 and abscisic acid (ABA) at 10 mg·L-1 induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L-1 IAA. The results indicate that in K. blossfeldiana, the aboveground parts of the plant play an important role in the biosynthesis of anthocyanins in roots.

Methyl jasmonate stimulates the formation and the accumulation of anthocyanin in Kalanchoe blossfeldiana

2003 ◽  
Vol 25 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Marian Saniewski ◽  
Marcin Horbowicz ◽  
Jerzy Puchalski ◽  
Junichi Ueda
Keyword(s):  
Methyl Jasmonate ◽  
Kalanchoe Blossfeldiana ◽  
Kalanchoë Blossfeldiana

scholarly journals The effect of sugars in relation to methyl jasmonate on anthocyanin formation in the roots of Kalanchoe blossfeldiana (Poelln.)

2015 ◽  
Vol 32 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Justyna Góraj-Koniarska ◽  
Marian Saniewski
Keyword(s):  
Methyl Jasmonate ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Sugar Alcohols ◽  
Anthocyanin Formation ◽  
Intact Plants ◽  
Kalanchoe Blossfeldiana ◽  
Kalanchoë Blossfeldiana

<p>This study investigated the effects of different sugars (sucrose, fructose, glucose) and sugar alcohols (mannitol, sorbitol) applied alone and in solution with methyl jasmonate (JA-Me) on the anthocyanin content in the roots of <em>Kalanchoe blossfeldiana</em>. None of the sugars used individually in the experiment affected anthocyanin accumulation in the roots of intact plants. The anthocyanin level was similar to that in the control. Sucrose at concentrations of 0.5% and 3.0%, and glucose at a concentration of 3.0% inhibited anthocyanin accumulation induced by JA-Me. Only fructose at a concentration of 3.0% stimulated anthocyanin accumulation induced by JA-Me. The sugar alcohols, mannitol at a concentration of 3.0% and sorbitol at 0.5% and 3.0%, inhibited anthocyanin accumulation in the roots of intact <em>K. blossfeldiana</em> plants induced by JA-Me. In excised roots, both sugars and JA-Me used individually did not affect the formation of anthocyanins. Also, the sugar alcohols (mannitol and sorbitol) applied simultaneously with JA-Me had no effect on the accumulation of anthocyanins. However, roots treated with sugars (sucrose, fructose, glucose) in solution with JA-Me promoted the induction of anthocyanins in the apical parts of the roots. </p><p>The results suggest that anthocyanin elicitation in the roots of <em>K. blossfeldiana</em> by methyl jasmonate may be dependent on the interaction of JA-Me with sugars transported from the stems (leaves) to the roots.</p>

scholarly journals Elicitation of Anthocyanin Production in Roots of Kalanchoe blossfeldiana by Methyl Jasmonate

2015 ◽  
Vol 57 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Justyna Góraj-Koniarska ◽  
Anna Stochmal ◽  
Wiesław Oleszek ◽  
Jarosław Mołdoch ◽  
Marian Saniewski
Keyword(s):  
Methyl Jasmonate ◽  
Natural Light ◽  
Anthocyanin Accumulation ◽  
Light Conditions ◽  
Intact Plants ◽  
Kalanchoe Blossfeldiana ◽  
Isolated Roots ◽  
Anthocyanin Production ◽  
Kalanchoë Blossfeldiana

AbstractThe influence of methyl jasmonate on anthocyanin accumulation in roots ofKalanchoe blossfedianaplants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l−1, substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants ofK. blossfeldianathan in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of oldK. blossfeldianaplants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Free Living ◽  
Ω3 Fatty Acids ◽  
Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

EFFECT OF GINGER, LEMURU FISH OIL SAPONIFICATION AND OLIVE OIL ON COMPOSITION FATTY ACID OF BEEF

2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fish Oil ◽  
Olive Oil ◽  
Rice Bran ◽  
Dry Matter ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Omega 3 ◽  
Omega 6

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 

Fatty Acid Profile and In Vitro Anticancer Activity of Two Marine Sponge- Associated Bacteria

2020 ◽  
Vol 16 (9) ◽  
pp. 1273-1280
Author(s):  
Giuseppina Tommonaro ◽  
Ali M. El-Hagrassi ◽  
Walid Fayad ◽  
Carmine Iodice ◽  
Kamel H. Shaker ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Colorectal Carcinoma ◽  
Cell Line ◽  
Marine Sponge ◽  
Hct116 Cell ◽  
Associated Bacteria ◽  
Sponge Associated Bacteria ◽  
Human Colorectal Carcinoma

Background: Colorectal cancer represents one of the prominent causes of mortality worldwide in men and women. The objective of this study was to search for new potential anticancer compounds, both in prevention and treatment of colorectal cancer. The anticancer potential of marine bacterial extracts against Human colorectal carcinoma cell line (HCT116) was evaluated as well as the partial identification of bioactive metabolites. Methods: All bacterial extracts were tested for their cytotoxicity against HCT116 cell line by means of MTT assay. The highly cytotoxic dichloromethane extracts of marine sponge-associated bacteria Vibrio sp. and Bacillus sp. were analyzed by GC-MS. Results: Two fractions, Vib3 and Bac3, exhibited a very interesting cytotoxicity against human colorectal carcinoma (HCT116) cell line, with a percentage of cytotoxicity of 96.04 % and 29.48 %, respectively. Discussion: The GC-MS analysis revealed the presence of two major fatty acids, palmitic and oleic acids, in Vib3 fraction and fatty acid esters and phenolic compounds in Bac3 fraction. Conclusion: Based on previous literature, it may be hypothesized that the anticancer activity of bacterial extracts could be, at least partially, to the fatty acids fraction.

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