scholarly journals The effect of Ethrel on detection of potato virus Y (PVYº) in primarily infected potato plants

2013 ◽  
Vol 41 (2) ◽  
pp. 257-264
Author(s):  
Marian Staszewicz
Keyword(s):  
Statistical Analysis ◽  
Potato Virus ◽  
Active Ingredient ◽  
Potato Virus Y ◽  
Elisa Test ◽  
Field Conditions ◽  
Average Deviation ◽  
Potato Plants ◽  
Control Variant ◽  
And Control

The effect of ethylene (in the form of Ethrel) on the detection of PVYº in cv. Kaszubski potatoes primarily infected in field conditions, was studied. The plants were inoculated with PVYº and sprayed with Ethrel at a concentration of 100 mg/dm<sup>3</sup>, 10 cm<sup>3</sup> of solution were used per plant. The concentration of the active ingredient, CEPA, in this amount of solution was 1 mg/plant. The extinction value and PVYº detection were assessed in the leaf and tuber juices from Ethrel-treated plants and control variant. The ELISA test was used. The results were subjected to statistical analysis and the average deviation at <i>P</i> = 0.05 was calculated. It was shown that Ethrel lowered the value of extinction and decreased PVYº detection in the leaves and tubers of potato plants of the Kaszubski variety primarily infected in field conditions.

scholarly journals Resistance of genetically modified potatoes to Potato virus Y under field conditions

2009 ◽  
Vol 44 (9) ◽  
pp. 1127-1130 ◽  
Author(s):  
André Nepomuceno Dusi ◽  
Cristiane Lopes de Oliveira ◽  
Paulo Eduardo de Melo ◽  
Antonio Carlos Torres
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Protein Gene ◽  
Genetically Modified ◽  
Growth Cycle ◽  
Field Conditions ◽  
Potato Plants ◽  
The Third ◽  
Genetically Modified Potatoes ◽  
Das Elisa

The objective of this work was to evaluate the resistance of genetically modified clones of potato to Potato virus Y (PVY) under field conditions. Genetically modified plants were compared with nontransformed plants of the same cultivar. The plots were flanked with potato plants infected with both PVYº and PVY N strains (spread lines), in order to provide the experimental area with the source of virus, which was naturally spread by the native aphid population. The experiment was weekly monitored by visual inspections and by DAS-Elisa in the plants produced from the harvested tubers, in order to evaluate the resistance of transgenic plants throughout the plant growth cycle. By the end of the third year, no infection symptoms were observed in the 1P clone; clone 63P showed 1% of infection, in contrast to about 90% of nontransformed plants infected. The stable expression of resistance to PVY provided by the coat protein gene was obtained in genetically modified clones of potato plants cultivar Achat under field conditions, during three consecutive years.

Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y

1999 ◽  
Vol 55 (3) ◽  
pp. 143-150 ◽  
Author(s):  
J. HINRICHS-BERGER ◽  
M. HARFOLD ◽  
S. BERGER ◽  
H. BUCHENAUER
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Plants

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

scholarly journals Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

2020 ◽  
Vol 22 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Igor Fesenko ◽  
Nadezhda Spechenkova ◽  
Anna Mamaeva ◽  
Antonida V. Makhotenko ◽  
Andrew J. Love ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Temperature Sensitive ◽  
Potato Plants ◽  
Methionine Cycle

Quantification of mineral oil accumulation and movement in potato plants and its significance in potato virus Y management

2013 ◽  
Vol 70 (8) ◽  
pp. 1243-1248 ◽  
Author(s):  
Manphool Fageria ◽  
Sébastien Boquel ◽  
Gaetan Leclair ◽  
Yvan Pelletier
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Mineral Oil ◽  
Oil Accumulation ◽  
Potato Plants

Role of Hairy Nightshade in the Transmission of Different Potato virus Y Strains on Solanum tuberosum (L.)

2010 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Felix A. Cervantes ◽  
Juan M. Alvarez
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Solanum Tuberosum L ◽  
Macrosiphum Euphorbiae ◽  
Invasive Weed ◽  
Potato Plants ◽  
Tuber Necrosis

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

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