scholarly journals Chromosomal karyotype in chorionic villi of recurrent spontaneous abortion patients

2018 ◽  
Vol 12 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Yan Du ◽  
Lanting Chen ◽  
Jing Lin ◽  
Jun Zhu ◽  
Na Zhang ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Recurrent Spontaneous Abortion ◽  
Chorionic Villi ◽  
Chromosomal Karyotype

Circular RNAs expressed in chorionic villi are probably involved in the occurrence of recurrent spontaneous abortion

2017 ◽  
Vol 88 ◽  
pp. 1154-1162 ◽  
Author(s):  
Yating Qian ◽  
Xin Wang ◽  
Hongjie Ruan ◽  
Can Rui ◽  
Pengyuan Mao ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Recurrent Spontaneous Abortion ◽  
Circular Rnas ◽  
Chorionic Villi

scholarly journals Spontaneous Abortion and Chikungunya Infection: Pathological Findings

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 554
Author(s):  
Natália Salomão ◽  
Michelle Brendolin ◽  
Kíssila Rabelo ◽  
Mayumi Wakimoto ◽  
Ana Maria de Filippis ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Spontaneous Abortion ◽  
Ultrastructural Changes ◽  
Rt Pcr ◽  
Chorionic Villi ◽  
Decidual Cells ◽  
Hofbauer Cells ◽  
Hematoxylin And Eosin ◽  
Maternal Fetal Transmission

Intrauterine transmission of the Chikungunya virus (CHIKV) during early pregnancy has rarely been reported, although vertical transmission has been observed in newborns. Here, we report four cases of spontaneous abortion in women who became infected with CHIKV between the 11th and 17th weeks of pregnancy. Laboratorial confirmation of the infection was conducted by RT-PCR on a urine sample for one case, and the other three were by detection of IgM anti-CHIKV antibodies. Hematoxylin and eosin (H&E) staining and an electron microscopy assay allowed us to find histopathological, such as inflammatory infiltrate in the decidua and chorionic villi, as well as areas of calcification, edema and the deposition of fibrinoid material, and ultrastructural changes, such as mitochondria with fewer cristae and ruptured membranes, endoplasmic reticulum with dilated cisterns, dispersed chromatin in the nuclei and the presence of an apoptotic body in case 1. In addition, by immunohistochemistry (IHC), we found a positivity for the anti-CHIKV antibody in cells of the endometrial glands, decidual cells, syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and decidual macrophages. Electron microscopy also helped in identifying virus-like particles in the aborted material with a diameter of 40–50 nm, which was consistent with the size of CHIKV particles in the literature. Our findings in this study suggest early maternal fetal transmission, adding more evidence on the role of CHIKV in fetal death.

scholarly journals Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiao-Hui Wang ◽  
Sha Xu ◽  
Xiang-Yu Zhou ◽  
Rui Zhao ◽  
Yan Lin ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Embryo Implantation ◽  
Underlying Mechanism ◽  
Recurrent Spontaneous Abortion ◽  
Extravillous Trophoblast ◽  
Trophoblast Invasion ◽  
Iron Sulfur ◽  
Succinate Dehydrogenase Complex ◽  
Extravillous Trophoblasts ◽  
Control Study

AbstractDysregulated extravillous trophoblast invasion and proliferation are known to increase the risk of recurrent spontaneous abortion (RSA); however, the underlying mechanism remains unclear. Herein, in our retrospective observational case-control study we show that villous samples from RSA patients, compared to healthy controls, display reduced succinate dehydrogenase complex iron sulfur subunit (SDHB) DNA methylation, elevated SDHB expression, and reduced succinate levels, indicating that low succinate levels correlate with RSA. Moreover, we find high succinate levels in early pregnant women are correlated with successful embryo implantation. SDHB promoter methylation recruited MBD1 and excluded c-Fos, inactivating SDHB expression and causing intracellular succinate accumulation which mimicked hypoxia in extravillous trophoblasts cell lines JEG3 and HTR8 via the PHD2-VHL-HIF-1α pathway; however, low succinate levels reversed this effect and increased the risk of abortion in mouse model. This study reveals that abnormal metabolite levels inhibit extravillous trophoblast function and highlights an approach for RSA intervention.

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