scholarly journals Key role of liver sinusoidal endothelial cells in liver fibrosis

2017 ◽  
Vol 11 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Mingxing Xu ◽  
Xuehua Wang ◽  
Yong Zou ◽  
Yuesi Zhong
Keyword(s):  
Endothelial Cells ◽  
Liver Fibrosis ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells

scholarly journals Inhibition of liver sinusoidal endothelial cells in rats with hepatic fibrosis by injection of VEGF 165 via the inferior vena cava

2021 ◽  
Author(s):  
Zhigang Sun ◽  
Tianyi Dong ◽  
Zhun Zhang ◽  
Tiantian Wang ◽  
Chenyu Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endothelial Cells ◽  
Liver Fibrosis ◽  
Serum Level ◽  
Basement Membranes ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Transmission Electron

Abstract Background Although VEGF can maintain the normal phenotype of liver sinusoidal endothelial cells (LSECs), it has also been reported that VEGF exacerbates cirrhosis. The role of VEGF in the progression and recovery of cirrhosis has still remained controversial.Methods We established a cirrhotic rat model by thioacetamide that was used as drinking water; besides, 0, 1, 2, and 4 μg VEGF165 were then continuously injected into the rats. The serum level of hyaluronic acid was measured by ELISA at 0, 1, and 4 weeks, separately. Serum levels of ALT, AST, direct bilirubin, indirect bilirubin, and ALB were detected by an automatic biochemical analyzer. In addition, the levels of VEGF165, CD44, MMP9, MMP2, HIF-1α, and endothelin were detected by Western blotting. The expression level of CD44 in LSECs was detected by immunohistochemistry. Changes of fenestrations of LSECs and basement membranes of blood vessels were observed by transmission electron microscopy. Results With the increase of dosage and duration of VEGF treatment, the levels of liver function markers in the serum, the levels of CD44, HIF-1α, hydroxyproline and endothelin were significantly improved. With determination of the serum level of hydroxyproline in the blood, it was disclosed that the mentioned level was markedly decreased. In the Sirius Red staining, the stained red area was gradually reduced. Images captured by transmission electron microscopy also confirmed that the ultrastructure of LSECs tended to be normal.Conclusion VEGF165 can accelerate the resolution of liver fibrosis by promoting fenestration structure formation in LSECs, as well as promoting material exchange between sinusoids and hepatocytes. Our findings may provide a new insight for the study of the role of VEGF in liver fibrosis.

scholarly journals Role of Differentiation of Liver Sinusoidal Endothelial Cells in Progression and Regression of Hepatic Fibrosis in Rats

2012 ◽  
Vol 142 (4) ◽  
pp. 918-927.e6 ◽  
Author(s):  
Guanhua Xie ◽  
Xiangdong Wang ◽  
Lei Wang ◽  
Lin Wang ◽  
Roscoe D. Atkinson ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Hepatic Fibrosis ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells

scholarly journals SECs (Sinusoidal Endothelial Cells), Liver Microenvironment, and Fibrosis

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Vaishaali Natarajan ◽  
Edward N. Harris ◽  
Srivatsan Kidambi
Keyword(s):  
Endothelial Cells ◽  
Liver Fibrosis ◽  
Cell Activation ◽  
Stellate Cell ◽  
Protein Profile ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells ◽  
Fibrotic Liver ◽  
Nonalcoholic Fatty Liver ◽  
Functional Features

Liver fibrosis is a wound-healing response to chronic liver injury such as alcoholic/nonalcoholic fatty liver disease and viral hepatitis with no FDA-approved treatments. Liver fibrosis results in a continual accumulation of extracellular matrix (ECM) proteins and paves the way for replacement of parenchyma with nonfunctional scar tissue. The fibrotic condition results in drastic changes in the local mechanical, chemical, and biological microenvironment of the tissue. Liver parenchyma is supported by an efficient network of vasculature lined by liver sinusoidal endothelial cells (LSECs). These nonparenchymal cells are highly specialized resident endothelial cell type with characteristic morphological and functional features. Alterations in LSECs phenotype including lack of LSEC fenestration, capillarization, and formation of an organized basement membrane have been shown to precede fibrosis and promote hepatic stellate cell activation. Here, we review the interplay of LSECs with the dynamic changes in the fibrotic liver microenvironment such as matrix rigidity, altered ECM protein profile, and cell-cell interactions to provide insight into the pivotal changes in LSEC physiology and the extent to which it mediates the progression of liver fibrosis. Establishing the molecular aspects of LSECs in the light of fibrotic microenvironment is valuable towards development of novel therapeutic and diagnostic targets of liver fibrosis.

scholarly journals Pivotal role of liver sinusoidal endothelial cells in NAFLD/NASH progression

2015 ◽  
Vol 95 (10) ◽  
pp. 1130-1144 ◽  
Author(s):  
Masashi Miyao ◽  
Hirokazu Kotani ◽  
Tokiko Ishida ◽  
Chihiro Kawai ◽  
Sho Manabe ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Pivotal Role ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells
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