Determination Of Concentrations Of Human Serum Albumin In Phosphate Buffer Solutions Using Near-Infrared Spectroscopy In The Region Of 750-2500 Nm

10.5580/d71 ◽  
2010 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Infrared Spectroscopy ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Near Infrared Spectroscopy ◽  
Phosphate Buffer ◽  
Near Infrared ◽  
Buffer Solutions

Determination of Proteins in Human Serum by Near-Infrared Spectroscopy with Partial Least Square Analysis

2016 ◽  
Vol 49 (18) ◽  
pp. 2964-2976 ◽  
Author(s):  
Huixian Guo ◽  
Furong Huang ◽  
Yuanpeng Li ◽  
Tao Fang ◽  
Siqi Zhu ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Human Serum ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Partial Least Square ◽  
Least Square ◽  
Partial Least Square Analysis

scholarly journals A Near Infrared Fluorescent Probe for Sensitive Determination of Human Serum Albumin

2016 ◽  
Vol 32 (12) ◽  
pp. 1291-1294 ◽  
Author(s):  
Xiaodan ZENG ◽  
Mingshuo MA ◽  
Baocun ZHU ◽  
Lin ZHU
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Sensitive Determination

Multivariate Determination of Human Serum Albumin and γ-Globulin in a Phosphate Buffer Solution by near Infrared Spectroscopy

1998 ◽  
Vol 6 (1) ◽  
pp. 375-381 ◽  
Author(s):  
Koichi Murayama ◽  
Keiichi Yamada ◽  
Roumiana Tsenkova ◽  
Yan Wang ◽  
Yukihiro Ozaki
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Standard Error ◽  
Phosphate Buffer ◽  
Near Infrared ◽  
Phosphate Buffer Solution ◽  
Clinical Analysis ◽  
Pls Regression ◽  
Buffer Solution

Near infrared (NIR) spectra in the 1300–1850 nm region were measured for phosphate buffer solutions containing both human serum albumin and γ-globulin with various concentrations. The concentrations of albumin and γ-globulin were determined by partial least squares (PLS) regression analysis. The calibration for albumin in the concentration ranges of 0.100–8.000 g dL−1 yielded the correlation coefficient ( R) of 0.998, the standard error of calibration ( SEC) of 0.124 g dL−1, and the standard error of prediction ( SEP) of 0.152 g dL−1, respectively. Those for γ-globulin in the concentration ranges of 0.100–6.000 g dL−1 yielded were 0.998, 0.110 g dL−1 and 0.118 g dL−1, respectively. The regression coefficients ( RCs) of PLS factors for albumin were compared with those for γ-globulin. The differences were discussed for each RC between albumin and γ-globulin. The coefficient of variation ( CV) was calculated to be 0.0388 and 0.0374 for albumin and γ-globulin, respectively. The ratio of standard derivation of reference data in prediction set to SEP ( RPD) are 13.4 and 16.4 for them. These values obtained in the present study satisfy the demands of clinical analysis of blood. The present results demonstrate that it is possible to determine the concentrations of the two kinds of proteins in the solution simultaneously by use of NIR and PLS regression.

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