Isolation, Purification And Liberation Of Free Phosphate By Indigenous Phosphate Solubilizing Bacteria And Effect On Plant Growth Promotion.

10.5580/893 ◽  
2011 ◽  
Vol 9 (1) ◽  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp. S119

Symbiosis ◽  
2016 ◽  
Vol 72 (1) ◽  
pp. 31-43 ◽  
Author(s):  
Liliana Mercedes Ludueña ◽  
María Soledad Anzuay ◽  
Jorge Guillermo Angelini ◽  
Germán Barros ◽  
María Flavia Luna ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Pyrroloquinoline Quinone ◽  
Phosphate Solubilizing

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals Isolation of Phosphate Solubilizing Bacteria and their Use for Plant Growth Promotion in Tomato Seedling and Plant

2018 ◽  
Vol 13 (2) ◽  
pp. 61-70
Author(s):  
Rajiv Pathak ◽  
Vipassana Paudel ◽  
Anupama Shrestha ◽  
Janardan Lamichhane ◽  
Dhurva. P. Gauchan
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
In Vitro Study ◽  
Dry Weight ◽  
Greenhouse Experiment ◽  
Phosphate Solubilizing Bacteria ◽  
Tomato Seedling ◽  
Phosphate Solubilizing ◽  
Phosphorous Compounds

Phosphorous (P) is an essential macronutrient and most soils contain high levels of P. However, its availability to plant is limited by rapid immobilization of phosphorous compounds to insoluble forms and hence plant available forms of P in soils are found in low amounts. Phosphate solubilizing bacteria provide an eco-friendly alternative to convert insoluble phosphates into plant available forms. In the present study, three phosphate solubilizing bacterial isolates (PB-1, PB-4 and VC-01) with visually significant phosphate solubilizing abilities were isolated from tomato rhizosphere soil. In-vitro study in pikovskaya’s agar revealed that isolate PB-1 had the highest phosphate solubilizing ability with a phosphate solubilizing index of 2.08±0.07 followed by isolate VC-01 (1.31±0.09) and PB-4 (1.24±0.08). Isolates were used as bacterial inoculum to assess their ability to promote tomato (Lycopersicon esculentum var. Srijana) seedling and plant growth in in-vitro and greenhouse experiment respectively. Isolate PB-4 showed best growth promotion in seedling assay whereas isolate PB-1 and VC-01 also promoted seedling growth compared to control. In greenhouse experiment however, isolates VC-01 and PB-1 significantly enhanced all parameters (shoot length, root length, shoot and root dry weight) compared to uninoculated control whereas isolate PB-4 had a positive effect on all parameters except root length.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 61-70

scholarly journals Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N2-Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency

2019 ◽  
Vol 16 (13) ◽  
pp. 2442 ◽  
Author(s):  
Zhikang Wang ◽  
Ziyun Chen ◽  
Xiangxiang Fu
Keyword(s):  
Plant Growth ◽  
Soil Properties ◽  
Soil Amendment ◽  
Growth Promotion ◽  
Synergistic Effects ◽  
Available P ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Unsterilized Soil ◽  
Phosphate Solubilizing

The inoculation of beneficial microorganisms to improve plant growth and soil properties is a promising strategy in the soil amendment. However, the effects of co-inoculation with phosphate-solubilizing bacteria (PSB) and N2-fixing bacteria (NFB) on the soil properties of typical C-deficient soil remain unclear. Based on a controlled experiment and a pot experiment, we examined the effects of PSB (M: Bacillus megaterium and F: Pseudomonas fluorescens), NFB (C: Azotobacter chroococcum and B: Azospirillum brasilence), and combined PSB and NFB treatments on C, N, P availability, and enzyme activities in sterilized soil, as well as the growth of Cyclocarya Paliurus seedlings grow in unsterilized soil. During a 60-day culture, prominent increases in soil inorganic N and available P contents were detected after bacteria additions. Three patterns were observed for different additions according to the dynamic bacterial growth. Synergistic effects between NFB and PSB were obvious, co-inoculations with NFB enhanced the accumulation of available P. However, decreases in soil available P and N were observed on the 60th day, which was induced by the decreases in bacterial quantities under C deficiency. Besides, co-inoculations with PSB and NFB resulted in greater performance in plant growth promotion. Aimed at amending soil with a C supply shortage, combined PSB and NFB treatments are more appropriate for practical fertilization at intervals of 30–45 days. The results demonstrate that co-inoculations could have synergistic interactions during culture and application, which may help with understanding the possible mechanism of soil amendment driven by microorganisms under C deficiency, thereby providing an alternative option for amending such soil.

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