scholarly journals Lysinibacillus massiliensis Isolated from the Synovial Fluid: A Case Report

2020 ◽  
Vol 25 (4) ◽  
pp. 595-598
Author(s):  
Canan Eryıldız ◽  
Kıymet Tabakçıoğlu ◽  
Sezgin Kehaya ◽  
Nermin Şakru ◽  
Şaban Gürcan
Keyword(s):  
Synovial Fluid ◽  
Blood Culture ◽  
Blood Culture Bottle ◽  
Negative Staining ◽  
Phenotypic Characterization ◽  
Local Alignment ◽  
Identification System ◽  
Culture Bottle ◽  
Gram Negative ◽  
Microbiological Methods

Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium with peritrichous flagella belonging to the Bacillaceae family. A few cases of L. massiliensis isolated from the cerebrospinal fluid and tissue have been reported. In this study, we aimed to describe a case of L. massiliensis isolated from the synovial fluid. The synovial fluid from a 74-year-old female patient was inoculated into blood culture bottle. Gram-negative rods were observed in a gram-stained smear from a positive blood culture bottle. The bacterium was identified as Lysinibacillus sphaericus/Lysinibacillus fusiformis, with a probability of 89% using an automated bacterial identification system (VITEK2; Biomerieux, France). Subsequently, 16S rRNA gene sequencing was performed, and the sequence was analyzed using the Basic Local Alignment Search Tool. The sequence had 99.9% (1426/1427) identity with the strain L. massiliensis (GenBank ID: NR_043092.1). To our knowledge, this is the first reported case of L. massiliensis isolated from the synovial fluid. When an endospore-forming gram-negative staining bacterium can not be identified by phenotypic characterization, L. massiliensis should be considered, and different microbiological methods should be used for identification.

scholarly journals Floating fungus balls in a blood culture bottle

2021 ◽  
Author(s):  
Sophie Schneitler ◽  
Florian Bub ◽  
Sophia Benthien ◽  
Philipp M. Lepper ◽  
Oliver Kurzai ◽  
...  
Keyword(s):  
Blood Culture ◽  
Blood Culture Bottle ◽  
Culture Bottle ◽  
Fungus Balls

scholarly journals Blood Culture Bottle and Standard Culture Bottle Methods for Detection of Bacterial Pathogens in Parapneumonic Pleural Effusion

2015 ◽  
Vol 8 (10) ◽  
Author(s):  
Surapan Charoentunyarak ◽  
Sarassawan Kananuraks ◽  
Jarin Chindaprasirt ◽  
Panita Limpawattana ◽  
Kittisak Sawanyawisuth
Keyword(s):  
Pleural Effusion ◽  
Blood Culture ◽  
Bacterial Pathogens ◽  
Blood Culture Bottle ◽  
Culture Bottle ◽  
Parapneumonic Pleural Effusion ◽  
Standard Culture

scholarly journals Clinical Comparison of Nonvented Aerobic BacT/Alert Blood Culture Bottle and Standard Aerobic Bottle for Detection of Microorganisms in Blood

2000 ◽  
Vol 38 (10) ◽  
pp. 3864-3866 ◽  
Author(s):  
James W. Snyder ◽  
Karen S. Benzing ◽  
Gina K. Munier ◽  
Grace D. Bostic ◽  
Pamela S. Bozigar ◽  
...  
Keyword(s):  
Blood Culture ◽  
Blood Culture Bottle ◽  
Adult Patients ◽  
Culture Bottle ◽  
Blood Samples ◽  
Clinical Comparison

The current BacT/Alert standard aerobic (VA) blood culture bottle was redesigned and designated a nonvented aerobic (NVA) culture bottle; this bottle does not require venting. A total of 3,873 sets of blood samples for culture were obtained from adult patients with suspected bacteremia or fungemia. The NVA bottle showed performance equivalent to that of the VA bottle for recovery and speed of detection of microorganisms from blood without the need for venting the bottle.

scholarly journals Phenotypic Characterization and Antibiotic Resistance Patterns of Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Gram-Negative Bacteria in a Referral Hospital, Saudi Arabia

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mutasim E. Ibrahim ◽  
Mohammed Abbas ◽  
Abdullah M. Al-Shahrai ◽  
Bahaeldin K. Elamin
Keyword(s):  
Saudi Arabia ◽  
Phenotypic Characterization ◽  
Close Relative ◽  
Identification System ◽  
Gram Negative Bacteria ◽  
Major Health Problem ◽  
Gram Negative ◽  
Extended Spectrum ◽  
Resistance Rates ◽  
Esbl Producers

Background. Emergence of pathogenic bacteria carrying β-lactamase-resistant determinants has become a major health problem in the hospital setting. The study aimed to determine antibiotic-resistant patterns and frequency of extended-spectrum β-lactamase- (ESBL-) producing Gram-negative bacteria (GNB) and AmpC β-lactamase-producing GNB. Methodology. A prospective cross-sectional study was conducted during a period from September 2017 to August 2018 at King Abdullah Hospital, Bisha Province, Saudi Arabia. GNB (n = 311) were recovered from patients’ clinical specimens including sputum, urine, wound pus, blood, tracheal aspirates and high vaginal swabs, umbilical discharge, eye discharge, and cerebrospinal fluids. Isolates were identified by the Phoenix identification system. Antimicrobial susceptibility was tested by the Kirby–Bauer disk procedure. Phenotypic characterization of ESBLs and AmpC β-lactamases was performed utilizing the double-disk synergy test and inhibitor-based method, respectively. Associations with outcome measures were determined by simple descriptive statistics and a chi-square test. Results. Out of 311 GNB isolates, the frequency of ESBL and AmpC β-lactamase producers was 84 (27%) and 101 (32.5%), respectively. Klebsiella pneumoniae and Escherichia coli were common ESBL producers. AmpC β-lactamases predominate among Acinetobacter spp. and Pseudomonas aeruginosa. Coproduction of ESBLs and AmpC β-lactamases was found in 36 (11.6%) isolates, with very close relative frequencies among K. pneumoniae, Acinetobacter spp., and P. aeruginosa. β-Lactamase producers were predominantly found in the surgical department (56.5%) and ICUs (44.2%). ESBL producers revealed high resistance for cefuroxime (96.4%), cefotaxime (92.9%), and trimethoprim/sulfamethoxazole (90.5%). The resistance rates were significantly higher among ESBL producers than nonproducers for cephalosporins (p<0.001), amoxicillin/clavulanate (p<0.001), piperacillin/tazobactam (p=0.010), nitrofurantoin (p=0.027), aztreonam (p<0.001), ciprofloxacin (p=0.002), and trimethoprim/sulfamethoxazole (p<0.001). Significantly higher (p<0.05) resistance rates were observed among AmpC β-lactamase producers than nonproducers for all tested antibiotics. Conclusions. This finding showed a high prevalence of ESBL- and AmpC β-lactamase-producing GNB in our hospital. Quality control practice and routine detection of β-lactamase producers before deciding on antibiotic therapy are advocated.

Sign in / Sign up

Export Citation Format

Share Document