scholarly journals Association of Endothelial Nitric Oxide Synthase Gene Polymor-phisms with Early-Onset Ischemic Stroke in South Indians

2010 ◽  
Vol 17 (1) ◽  
pp. 45-53 ◽  
Author(s):  
Vijaya Majumdar ◽  
Dindagur Nagaraja ◽  
Nagaraja Karthik ◽  
Rita Christopher
Keyword(s):  
Nitric Oxide ◽  
Ischemic Stroke ◽  
Nitric Oxide Synthase ◽  
Early Onset ◽  
Endothelial Nitric Oxide Synthase ◽  
South Indians ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals G894T endothelial nitric oxide synthase polymorphism and ischemic stroke in Morocco

Meta Gene ◽  
2014 ◽  
Vol 2 ◽  
pp. 349-357 ◽  
Author(s):  
Brehima Diakite ◽  
Khalil Hamzi ◽  
Ilham Slassi ◽  
Mohammed EL Yahyaoui ◽  
Moulay M.F. EL Alaoui ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ischemic Stroke ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Relaxin Peptide Hormones Are Protective During the Early Stages of Ischemic Stroke in Male Rats

Endocrinology ◽  
2014 ◽  
Vol 156 (2) ◽  
pp. 638-646 ◽  
Author(s):  
Lindsay H. Bergeron ◽  
Jordan M. Willcox ◽  
Faisal J. Alibhai ◽  
Barry J. Connell ◽  
Tarek M. Saleh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ischemic Stroke ◽  
Nitric Oxide Synthase ◽  
Infarct Size ◽  
Endothelial Nitric Oxide Synthase ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Protective Mechanisms ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The pregnancy hormone relaxin protects tissue from ischemic damage. The ability of relaxin-3, a relaxin paralog, to do so has not been explored. The cerebral expression levels of these peptides and their receptors make them logical targets for study in the ischemic brain. We assessed relaxin peptide-mediated protection, relative relaxin family peptide receptor (RXFP) involvement, and protective mechanisms. Sprague-Dawley rats receiving permanent (pMCAO) or transient middle cerebral artery occlusions (tMCAO) were treated with relaxin peptides, and brains were collected for infarct analysis. Activation of the endothelial nitric oxide synthase pathway was evaluated as a potential protective mechanism. Primary cortical rat astrocytes were exposed to oxygen glucose deprivation and treated with relaxin peptides, and viability was examined. Receptor involvement was explored using RXFP3 antagonist or agonist treatment and real-time PCR. Relaxin and relaxin-3 reduced infarct size after pMCAO. Both peptides activated endothelial nitric oxide synthase. Because relaxin-3 has not previously been associated with this pathway and displays promiscuous RXFP binding, we explored the receptor contribution. Expression of rxfp1 was greater than that of rxfp3 in rat brain, although peptide binding at either receptor resulted in similar overall protection after pMCAO. Only RXFP3 activation reduced infarct size after tMCAO. In astrocytes, rxfp3 gene expression was greater than that of rxfp1. Selective activation of RXFP3 maintained astrocyte viability after oxygen glucose deprivation. Relaxin peptides are protective during the early stages of ischemic stroke. Differential responses among treatments and models suggest that RXFP1 and RXFP3 initiate different protective mechanisms. This preliminary work is a pivotal first step in identifying the clinical implications of relaxin peptides in ischemic stroke.

scholarly journals Endothelial Nitric Oxide Synthase Gene Polymorphism (G894T) and Diabetes Mellitus (Type II) among South Indians

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
T. Angeline ◽  
H. R. Krithiga ◽  
W. Isabel ◽  
A. J. Asirvatham ◽  
A. Poornima
Keyword(s):  
Nitric Oxide ◽  
Diabetes Mellitus ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Enzyme Analysis ◽  
Genotype Distribution ◽  
South Indians ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

The objective of the study is to find out whether the endothelial nitric oxide synthase (eNOS) G894T single-nucleotide polymorphism is associated with type 2 diabetes mellitus in South Indian (Tamil) population. A total number of 260 subjects comprising 100 type 2 diabetic mellitus patients and 160 healthy individuals with no documented history of diabetes were included for the study. DNA was isolated, and eNOS G894T genotyping was performed using the polymerase chain reaction followed by restriction enzyme analysis usingBan II. The genotype distribution in patients and controls were compatible with the Hardy-Weinberg expectations (P>0.05). Odds ratio indicates that the occurrence of mutant genotype (GT/TT) was 7.2 times (95% CI = 4.09–12.71) more frequent in the cases than in controls. Thus, the present study demonstrates that there is an association of endothelial nitric oxide synthase gene (G894T) polymorphism with diabetes mellitus among South Indians.

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