scholarly journals Comparison of Two Harvesting Methods for the Continuous Production of Indigo Plant (Polygonum Tinctorium) Leaves in a Closed-Cultivation System

2017 ◽  
Vol 6 (2) ◽  
pp. 7
Author(s):  
Masaru Sakamoto ◽  
Dong-An Kim ◽  
Keiko Imoto ◽  
Yusuke Kitai ◽  
Takahiro Suzuki

Although the blue dye indigo has been chemically synthesized for over a century, there is an increasing interest in the indigo plant (Polygonum tinctorium) as a source of natural dyes and medicines. To maintain a stable supply of P. tinctorium throughout the year, we examined the effect of two harvesting methods on the leaf yield of this plant under a closed-cultivation system. With method 8c-M, all shoot branches >8 cm of the stem bottom were harvested and under method 2b-M, all branches, but two, were harvested at the stem bottom. Both methods enabled sustainable leaf yields from the same plants over 1 year. The total weight of shoot branches harvested by 8c-M was 1.86–3.11 times higher that of shoot branches harvested by 2b-M. Harvesting by 8c-M resulted in shoot branch weights lower than those from plants harvested with the 2b-M. Leaf/shoot ratio was increased in plants harvested by 8c-M. The content of indican, the precursor of indigo, in leaves was not significantly different between the two harvesting methods. Our data may provide a new continuous cultivation method of leaf crops all over years in controlled-cultivation systems.

2004 ◽  
Vol 10 (3) ◽  
Author(s):  
Szalay L. ◽  
Molnár B. P.

The apricot is propagated on several kinds of rootstocks in Hungary. The main aspects of selecting rootstocks are as follows: adaptability to environmental circumstances, primarily soil conditions, ensuring the tree size that complies with the cultivation method, and compatibility with the grafted cultivar. At advanced, intensive orchards rootstocks ensuring smaller tree size are privileged. For the establishment of the appropriate cultivation system, it is important to be aware of the expected growing vigour and tree size of certain cultivar­rootstock combinations when the orchard is designed. In the course of our experiment the size of 15-year-old trees of 4 apricot cultivars were examined on several rootstocks at an orchard in Siófok. On the basis of the data measured for each cultivar-rootstock combination, it can be stated that trees on wild apricot (P. armeniaca) rootstocks are the largest in size. Trees on prune (P. domestica) rootstock have 10-15% smaller crown volume than the previous combination. Trees on bullace (P. insititia) rootstock have the smallest tree size and their crown volume is 30-50% smaller than that of the trees on P. armeniaca rootstock. Thus, the application of prune and bullace rootstocks is beneficial at intensive apricot orchards as the size of trees can be reduced by their usage. However, their compatibility with the cultivars and their adaptability to the ecological conditions of the production site have to be tested before applying them widely. In the course of our research incompatibility was not experienced for any of the cultivar-rootstock combinations examined. Nevertheless, the drought tolerance of the rootstocks examined showed significant differences. Trees on P. domestica or P. insititia rootstock requires more water than those on P. armeniaca rootstock, therefore, they have to be irrigated.


1995 ◽  
Vol 43 (2) ◽  
pp. 259-266
Author(s):  
A. Morin ◽  
N. H. Tran Trung ◽  
G. LaPointe ◽  
H. Dubeau

Biologia ◽  
2015 ◽  
Vol 70 (8) ◽  
Author(s):  
Yoshiko Minami ◽  
Bijaya Ketan Sarangi ◽  
Sanjog Tarachand Thul

AbstractIndigo is the most important blue dye for textile dyeing and is biosynthesized in Polygonum tinctorium. Some biochemical studies related to biosynthesis are available. However, genomic and transcriptome studies have not received sufficient attention. Here, we report de novo assembly of transcriptome datasets and its comprehensive analysis. A total of 60,395 unigenes were annotated using BLAST search against the different databases. At least 23,721 unigenes mapped onto different pathways using KEGG database. We found that 3,323 genes are involved in biosynthesis of secondary metabolites, 117 phenylalanine, tyrosine and tryptophan biosynthesis and 147 tryptophan metabolisms. Apart from this, indigo biosynthesis pathway genes viz., dioxygenase, monooxygenase, and glucosyltransferase have also been identified. Fourteen genes encoding cytochrome P450 monooxygenase, 26 glucoside dioxygenase, 9 UDP-glucose D-glucosyltransferase and 52 were β-D-glucosidase. These findings provide a foundation for further analysis of this pathway with potential to enhance the synthesis of indican in P. tinctorium


2021 ◽  
pp. 1-7
Author(s):  
Thanga roja Kaliappan ◽  
K. A. Murugesh ◽  
P. Mangammal ◽  
R. Shanmugam

Chitosan is biopolymer of glucosamine residues, nontoxic, biodegradable and friendly to environment and it also helps to enhance crop production due to their bioactivities. The present experiment was conducted to assess the efficacy of chitosan on the growth and yield related attributes of mulberry, Morus sp. with various concentrations such as 25, 50, 75, 100 and 125 ppm. The chitosan was sprayed thrice on 15, 30 and 45 days after pruning (DAP) on the foliage of three different mulberry varieties namely V1, MR2 and G4. The result revealed that, irrespective of varieties, the foliar application of chitosan at 75 ppm significantly increased the biometric as well as yield related attributes of mulberry over other concentrations. Among the three varieties, V1 showed more response to chitosan application than G4 and MR2. At 75 ppm, chitosan showed marked effect on VI variety of mulberry and significantly increased shoot length (170.67 cm), number of shoots per plant (9.03), number of leaves per shoot (29.48), leaf area (220.26 cm2) and leaf area index (2.72) were observed when compared with control. The application of chitosan at 75 ppm significantly enhanced the yield traits namely weight of 100 leaves (448.10 g), leaf shoot ratio (1.45) and leaf yield (14.01 MT/ha/harvest) in V1.


1995 ◽  
Vol 43 (2) ◽  
pp. 259-266 ◽  
Author(s):  
A. Morin ◽  
N. H. Tran Trung ◽  
G. LaPointe ◽  
H. Dubeau

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