scholarly journals A Pancreatic Lipase Inhibitory Activity by Mango (Mangifera indica) Leaf Methanolic Extract

2016 ◽  
Vol 5 (2) ◽  
pp. 72 ◽  
Author(s):  
Kimihisa Itoh ◽  
Kazuya Murata ◽  
Yuta Nakagaki ◽  
Ayaka Shimizu ◽  
Yusuke Takata ◽  
...  
Keyword(s):  
Natural Resource ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Methanolic Extract ◽  
Mangifera Indica ◽  
Porcine Pancreatic Lipase ◽  
Active Ingredients ◽  
Mango Leaves ◽  
Dark Green

<p>The objective of this study was to identify pancreatic lipase inhibitory active ingredients of mango leaves, and to examine a relationship between leaves maturation and pancreatic lipase inhibitory activity. A methanolic extract of old dark green mango leaves (OML-ext) showed a porcine pancreatic lipase inhibitory activity. The pancreatic lipase inhibitory activity of OML-ext was attributable to 3-C-<em>β</em>-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (<strong>2</strong>) and mangiferin (<strong>1</strong>). The pancreatic lipase inhibitory activity of young mango leaf extract was superior to that of old leaf extract. It was suggested that the activity is correlated with the content of <strong>2</strong> in these extract. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity.</p>

scholarly journals Pancreatic Lipase Inhibitory Activity of Citrus unshiu Leaf Extract

2019 ◽  
Vol 14 (9) ◽  
pp. 1934578X1987343
Author(s):  
Kimihisa Itoh ◽  
Tetsuya Matsukawa ◽  
Kazuya Murata ◽  
Ryota Nishitani ◽  
Momona Yamagami ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Citrus Fruit ◽  
Late Winter ◽  
Citrus Unshiu ◽  
Porcine Pancreatic Lipase ◽  
Utility Value ◽  
Flower Buds

In the cultivation of Citrus fruit, pruning in late winter after harvest is important to obtain excellent flower buds in the next spring and a rich harvest of superior fruit in the following winter; a large number of pruned leaves are usually discarded. The purpose of this study was to determine the utility value of pruned leaves of Citrus unshiu. A methanol extract of pruned C. unshiu leaves showed a porcine pancreatic lipase inhibitory activity. Fractionation of C. unshiu leaf extract (CUL-ext) followed by bibliographic and chromatographic analyses revealed that a part of the pancreatic lipase inhibitory activity of CUL-ext was attributable to nobiletin (1), rutin (2), and hesperidin (3). Thus, pruned C. unshiu leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity.

scholarly journals Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract

2017 ◽  
Vol 6 (2) ◽  
pp. 102 ◽  
Author(s):  
Kimihisa Itoh ◽  
Kazuya Murata ◽  
Nao Sakaguchi ◽  
Kohei Akai ◽  
Tomoka Yamaji ◽  
...  
Keyword(s):  
Advanced Glycation End Products ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Mangifera Indica ◽  
Inhibitory Effect ◽  
Advanced Glycation ◽  
Leaf Extracts ◽  
Glycation End Products ◽  
End Products ◽  
Dark Green

The purpose of this study was to examine an inhibitory effect of mango leaf extracts on advanced glycation end products (AGEs) formation and to identify these active ingredients, and also to investigate a relationship between leaves maturation and the inhibitory activity. A methanolic extract of old dark green mango leaf extract (OML-ext) exhibited an inhibitory activity of AGEs formation in nonenzymatic glycation of albumin. The inhibitory activity of OML-ext was attributable to 3-C-β-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (1), mangiferin (2) and chlorophyll. Inhibitory effect of young dark reddish brown mango leaf extract (YDL-ext) on AGEs formation was similar to that of OML-ext. The inhibitory activity of YDL-ext was attributable to 1 and 2, in addition, a part of the the activity of YDL-ext due to anthocyanins whose content is highest in young dark reddish brown mango leaves. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with inhibitory activity of AGEs formation.

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT AND ANTIMICROBICIDAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF COUROUPITA GUIANENSIS

2021 ◽  
pp. 203-206
Author(s):  
SUJATHA ILANGOVAN ◽  
THAVASUMANI P
Keyword(s):  
Inhibitory Activity ◽  
Leaf Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Preliminary Screening ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Couroupita Guianensis ◽  
Phytochemical Constituents ◽  
Antibacterial And Antifungal

Objective: The objective of the study was the preliminary screening of the methanolic leaf extract of Couroupita guianensis for various phytochemical constituents, its antioxidant, antibacterial, and antifungal activities. Methods: Phytochemical screening of 18 qualitative, 6 quantitative constituents, antioxidant activity, alpha-amylase inhibitory activity, and alpha-glucosidase inhibitory activity of the methanolic extract of the leaf of C. guianensis was performed adopting the standard protocols. The disk diffusion methods were used for assessing the antibacterial and antifungal activities of the extract. Results: The preliminary studies revealed the presence of alkaloids, saponin, flavonoids, phenol, tannin, and terpenoids in the methanolic extract of the leaf of C. guianensis. Potent antioxidant, free radical scavenging activity, and inhibitory activity against α-amylase and α-glucosidase activity of the methanolic extract were also evident. Conclusions: The preliminary studies in the methanolic extract of the leaf of C. guianensis are suggestive of the therapeutic potentials of the methanolic extract of leaves of C. guianensis.

scholarly journals BIOLOGICAL ACTIVITIES OF NOVEL IN VITRO RAISED STEVIA PLANT

2017 ◽  
Vol 10 (8) ◽  
pp. 240 ◽  
Author(s):  
Neeta Raj Sharma ◽  
Vineet Meshram ◽  
Mahiti Gupta
Keyword(s):  
Pancreatic Lipase ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Activity ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Gram Negative Bacteria ◽  
Herbal Formulation ◽  
Further Development

  Objective: This communication explores a lead fraction from methanolic extract of novel Stevia species raised under in vitro conditions for its various biological activities.Methods: The dried Stevia leaves were crushed in methanol to get the polar extract. This methanol extract was tested for pancreatic lipase and alpha-amylase inhibitory activity using quantitative plate assays. Antibacterial property of the extract was also evaluated against Staphylococcus epidermidis, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa. Further, the antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl.Results: The methanolic extract inhibited pancreatic lipase with IC50 of 5.74 μg/ml in a similar manner to a well-known anti-obesity drug in the market orlistat. The methanolic extract also showed a better pancreatic α-amylase inhibitory activity (IC50=88 μg/ml) than acarbose. Further, the lead fraction exhibited 88.48% antioxidant activity. It also exhibited broad spectrum antimicrobial activity against the spectrum of Gram-positive and Gram-negative bacteria tested under laboratory conditions with a minimal inhibitory concentration ranging from 1.95 to 31.25 μg/ml.Conclusion: Thus, this study signifies the vast potential of the lead fraction from a novel Stevia species for further development into a herbal formulation for prevention of various infectious and non-infectious diseases.

scholarly journals Porcine Pancreatic Lipase Inhibitory Agent Isolated from Medicinal Herb and Inhibition Kinetics of Extracts from Eleusine indica (L.) Gaertner

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Siew Ling Ong ◽  
Siau Hui Mah ◽  
How Yee Lai
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Hexane Fraction ◽  
Inhibition Mechanism ◽  
Porcine Pancreatic Lipase ◽  
Active Components ◽  
Reference Drug ◽  
Spectral Techniques ◽  
Eleusine Indica ◽  
Kinetics Of

Eleusine indica (Linnaeus) Gaertner is a traditional herb known to be depurative, febrifuge, and diuretic and has been reported with the highest inhibitory activity against porcine pancreatic lipase (PPL) among thirty two plants screened in an earlier study. This study aims to isolate and identify the active components that may possess high potential as an antiobesity agent. Of the screened solvent fractions of E. indica, hexane fraction showed the highest inhibitory activity of 27.01±5.68% at 100 μg/mL. Bioactivity-guided isolation afforded three compounds from the hexane fraction of E. indica, namely, β-sitosterol, stigmasterol, and lutein. The structures of these compounds were elucidated using spectral techniques. Lutein showed an outstanding inhibitory activity against PPL (55.98±1.04%), with activity 60% higher than that of the reference drug Orlistat. The other compounds isolated and identified were β-sitosterol (2.99±0.80%) and stigmasterol (2.68±0.38%). The enzyme kinetics of E. indica crude methanolic extract on PPL showed mixed inhibition mechanism.

scholarly journals Quality Testing of Solid Soap with Mango Leaf Extract (Mangifera indica L.) Produced by Hot and Cold Processes

2020 ◽  
Vol 3 ◽  
pp. 171-173 ◽  
Author(s):  
Zulfa Nurul Karimah ◽  
Riska Kristiana
Keyword(s):  
Leaf Extract ◽  
Mangifera Indica ◽  
Average Quality ◽  
Soap Analysis ◽  
Foaming Agent ◽  
Heat Process ◽  
Saponin Content ◽  
Quality Testing ◽  
Mango Leaves

Mango leaves (Mangifera indica L.) contain alkaloids, flavonoids, stereoid compounds, polyphenols, tannins and saponins. The saponin content in mango leaves is able to act as a natural foaming agent. Mango leaf extract can be added in the manufacture of solid soap to produce natural soap. Making solid soap can be done through heating (heat process) and without heating (cold process). This study aims to determine the process of making solid soap in a hot and cold process, comparing the best quality soap analysis based on quality including organoleptic testing and pH balance. The results showed the average quality of the best soap through the heat process with the results of organoleptic tested.

scholarly journals α-Glucosidase and Pancreatic Lipase Inhibitory Activities of Diterpenes from Indian Mango Ginger (Curcuma amada Roxb.) and Its Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4071 ◽  
Author(s):  
Yuri Yoshioka ◽  
Naori Yoshimura ◽  
Shinichi Matsumura ◽  
Hiroto Wada ◽  
Maya Hoshino ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Parent Compound ◽  
Molecular Size ◽  
Porcine Pancreatic Lipase ◽  
Mango Ginger ◽  
Curcuma Amada ◽  
Structure Activity ◽  
Inhibitory Activities ◽  
Derivatives Of

Enzymatic inhibitions of crude extracts and their constituents from Zingiberaceae against both rat intestinal α-glucosidase and porcine pancreatic lipase were investigated. Structure–activity relationships using their derivatives were also investigated. The rhizomes extract of mango ginger, Curcuma amada showed remarkable inhibitory activity in the screening test. Two natural labdane diterpenes 1 and 2 and a drimane sesquiterpene 3 were major constituents isolated from this hexane extract. Among them, (E)-labda-8(17),12-diene-15,16-dial (1) was the most prominent compound and showed inhibitory activity against both α-glucosidase and lipase. Derivatives 4–10 from compound 1 were prepared and evaluated using inhibitory assays with these enzymes. The reduced derivative 4 maintained α-glucosidase inhibitory activity, but had decreased pancreatic lipase inhibitory activity compared with parent compound 1. Other tested derivatives of compound 1, including acetates 5–7 and oxidative derivatives 8–10, had very weak α-glucosidase inhibitory activity. Most of these compounds showed moderate pancreatic lipase inhibitory activity. However, only sesquiterpene albicanal (3) showed drastically decreased pancreatic lipase activity compared with 1. These findings suggested that molecular size was essential for enzymatic inhibitory activities of these compounds. These results demonstrated that mango ginger may be useful for the prevention of obesity and being overweight.

scholarly journals Phytochemical and Biological Profile of Moricandia arvensis (L.) DC.: An Inhibitor of Pancreatic Lipase

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2829 ◽  
Author(s):  
Mariangela Marrelli ◽  
Federica Morrone ◽  
Maria Argentieri ◽  
Lucia Gambacorta ◽  
Filomena Conforti ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Dietary Lipids ◽  
Lipid Absorption ◽  
Flavonoid Glycosides ◽  
Moricandia Arvensis ◽  
Ic50 Values

Pancreatic lipase, a key enzyme for lipid absorption, is one of the most important targets for the treatment of obesity, while natural compounds have recently attracted much interest as potential inhibitors of this enzyme. Here, in an attempt to find new effective agents, the methanolic extract from Moricandia arvensis (L.) DC. and its sub-extracts were investigated for their potential inhibitory activity. The ability to inhibit pancreatic lipase was verified through the in vitro evaluation of the prevention of p-nitrophenyl caprylate hydrolysis. The antioxidant activity was also verified by means of DPPH and β-carotene bleaching tests. Compositional profiling revealed that flavonoid glycosides were the main specialized metabolites present in the methanolic extract from the aerial parts of the plant with kaempferol and quercetin representing the two O-glycosylated aglycones. Kaempferol-3-O-β-(2″-O-glucosyl)-rutinoside and kaempferol-3-O-a-arabinosyl-7-O-rhamnoside were the most abundant flavonols. The crude methanolic extract and the dichloromethane and ethyl acetate sub-extracts showed a strong lipase inhibitory activity, with IC50 values of 2.06 ± 0.02, 1.52 ± 0.02 and 1.31 ± 0.02 mg/mL, respectively. The best capacity to scavenge DPPH radical was detected for the ethyl acetate sub-extract (IC50 = 171.9 ± 1.0 µg/mL), which was also effective in protecting linoleic acid from peroxidation (IC50 = 35.69 ± 2.30 µg/mL). Obtained results support the hypothesis that M. arvensis can be a source of bioactive phytochemicals for the pharmacological inhibition of dietary lipids absorption.

PANCREATIC LIPASE INHIBITORY AND ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF FLORETS OF ASTER SPECIES

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (12) ◽  
pp. 60-68
Author(s):  
R. R Shahu ◽  
◽  
P. Rathod ◽  
C Kulkarni ◽  
S. Mhatre ◽  
...  
Keyword(s):  
Free Radical ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Superoxide Radical ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Pancreatic lipase inhibitory effect of methanolic extract of florets Aster sp. was studied. In case of Aster sp. (White florets) the pancreatic lipase inhibition recorded was 82.75% with an IC50 value of 38μg/mL. Aster sp. (light pink) showed 80.04 % inhibition with an IC50 of 72 μg/mL. On 2 h dialysis, both the extracts lost their significant pancreatic lipase inhibitory activity, indicating the reversible nature of inhibition. pH slightly affected the performance of pancreatic lipase inhibitory activity. Extract (white florets) also showed 22.03% superoxide radical scavenging activity and 22.73% free radical scavenging activity indicating the multifunctional nature of the extract. Light pink florets showed 40.67% superoxide radical scavenging activity and 24.10% free radical scavenging activity. Saponin and alkaloid molecule in white florets of Aster sp. and alkaloid in light pink florets were identified as active principles for pancreatic lipase inhibitory activity.

ANTI-DIABESITY PRINCIPLE FROM THE SEEDS OF PHYLLANTHUS EMBLICA L.

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (12) ◽  
pp. 41-50
Author(s):  
Priyanka Rathod ◽  
Chandana Kulkarni ◽  
Raman P. Yadav
Keyword(s):  
Antioxidant Activity ◽  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Scavenging Activity ◽  
Phyllanthus Emblica ◽  
Natural Substrate ◽  
Glucosidase Inhibitor ◽  
Pancreatic Lipase Inhibition ◽  
Lipase Inhibition

In recent years, pancreatic lipase inhibitor and α- glucosidase inhibitor have been highlighted as potential anti-diabesity principles. In the present study, seeds of Phyllanthus emblica L. (Family: Phyllanthaceae) was studied for anti-diabesity potential in terms of pancreatic lipase inhibitory activity, α-glucosidase inhibitory activity and antioxidant activity. At 100μg/ml concentration, pancreatic lipase inhibition of the methanolic extract using synthetic substrate obtained was 73.2±0.1% (IC50 59.1μg/ml), whereas pancreatic lipase inhibition using natural substrate was 87.9 ± 2.62%. α- glucosidase inhibition of the extract at 50μg/ml was measured as 94.4±0.37% (IC50 34.4μg/ml). The superoxide scavenging activity of the extract was found to be 81.5±0.41%. Interestingly, upon TLC fingerprinting, only one band with Rf 0.70 showed multifunctional activity. The phytochemical found to be present was an alkaloid. The results evidenced the presence of multifunctional smart molecule in methanolic extract of P. emblica L and showed an alkaloid as the component responsible for anti-diabesity potential.

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