scholarly journals Effect of Gamma Irradiation on Aflatoxins and Ochratoxin A Reduction in Almond Samples

2014 ◽  
Vol 3 (4) ◽  
pp. 113 ◽  
Author(s):  
Di Stefano Vita ◽  
Pitonzo Rosa ◽  
Avellone Giuseppe
Keyword(s):  
Gamma Irradiation ◽  
Ochratoxin A ◽  
Gamma Rays ◽  
High Performance ◽  
Animal Health ◽  
Chromatography Method ◽  
Maximum Reduction ◽  
Immunoaffinity Columns ◽  
Liquid Chromatography Method ◽  
Gamma Radiations

<p>The widespread contamination of food by mycotoxins may present a serious hazard to human and animal health. The gamma rays were applied to reduce ochratoxin A (OTA) and aflatoxins B<sub>1</sub>, B<sub>2</sub>, G<sub>1</sub> and G<sub>2 </sub>(AFB<sub>1</sub>, AFB<sub>2</sub>, AFG<sub>1</sub> and AFG<sub>2</sub>) in almonds artificially contaminated. In the present study we investigated the effect of gamma irradiation dosages, ranging from 0 to 15 kGy and the reduction of mycotoxins concentration in almond samples. In order to determine the efficiency of the method, a high-performance liquid chromatography method with fluorescence detection was used, the mycotoxins were extracted from almond samples and then purified with immunoaffinity columns. The maximum reduction was found at 15 kGy and it was 19.25%, 10.99%, 21.11%, 16.62%, 23.90% for AFB<sub>1</sub>, AFB<sub>2</sub>, AFG<sub>1</sub>, AFG<sub>2</sub> and OTA respectively. Results showed that gamma radiations even at 15 kGy, were not effective in completely destroying aflatoxins and ochratoxin A.</p>

scholarly journals Assessment of Ochratoxin A Exposure in Ornamental and Self-Consumption Backyard Chickens

2020 ◽  
Vol 7 (1) ◽  
pp. 18 ◽  
Author(s):  
Alessandro Guerrini ◽  
Alberto Altafini ◽  
Paola Roncada
Keyword(s):  
Ochratoxin A ◽  
High Performance ◽  
Animal Origin ◽  
Chromatography Method ◽  
Italian Regions ◽  
Food And Feed ◽  
Backyard Chickens ◽  
Liquid Chromatography Method ◽  
Kidney Liver ◽  
Bile Samples

Ochratoxin A (OTA) is a mycotoxin that may be present in various food and feed of plant and animal origin, including chicken meat. In Italy, backyard poultry farming is rather widespread. Animals are raised for meat, eggs and for ornamental purpose, and they are often fed with home-made diets not subject to official controls. The purpose of this study was to evaluate exposure of ornamental and backyard chickens to OTA using biliary ochratoxin A as a biomarker. Therefore, bile samples, in addition to kidney, liver and muscle, were collected from 102 chickens reared in 16 farms located in 6 Italian regions. High-performance liquid chromatography method and fluorimetric detection (HPLC-FLD) analysis were carried out firstly on bile from all animals, and OTA was detected in 12 chickens (concentration range 3.83–170.42 µg/L). Subsequently, the kidneys of these chickens were also analysed, and the mycotoxin was not detected. The analytical detection limits (LODs) of OTA in bile and kidney were 2.1 µg/L and 0.1 µg/kg, respectively. In conclusion, these animals were exposed to OTA but their meat can be considered safe, given that this mycotoxin, if present, concentrates highest in kidneys. Biliary ochratoxin A confirms its use as a valid biomarker to assess exposure of poultry to OTA.

scholarly journals HPLC Quantification of Cytotoxic Compounds fromAspergillus niger

2017 ◽  
Vol 2017 ◽  
pp. 1-7
Author(s):  
Paula Karina S. Uchoa ◽  
Leandro Bezerra de Lima ◽  
Antonia T. A. Pimenta ◽  
Maria da Conceição F. de Oliveira ◽  
Jair Mafezoli ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Standard Deviation ◽  
High Performance ◽  
Chromatography Method ◽  
Relative Standard ◽  
Validation Parameters ◽  
Cytotoxic Compounds ◽  
Liquid Chromatography Method ◽  
Marine Strain

A high-performance liquid chromatography method was developed and validated for the quantification of the cytotoxic compounds produced by a marine strain ofAspergillus niger. The fungus was grown in malt peptone dextrose (MPD), potato dextrose yeast (PDY), and mannitol peptone yeast (MnPY) media during 7, 14, 21, and 28 days, and the natural products were identified by standard compounds. The validation parameters obtained were selectivity, linearity (coefficient of correlation > 0.99), precision (relative standard deviation below 5%), and accuracy (recovery > 96).

scholarly journals A stability-indicating high performance liquid chromatography method to determine apocynin in nanoparticles

2017 ◽  
Vol 7 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Juliana Kovalczuk de Oliveira ◽  
Débora Fernanda Veres Ronik ◽  
Jociani Ascari ◽  
Rubiana Mara Mainardes ◽  
Najeh Maissar Khalil
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chromatography Method ◽  
Stability Indicating ◽  
Liquid Chromatography Method
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