scholarly journals Use of Essential Oils as Natural Food Preservatives: Effect on the Growth of Salmonella Enteritidis in Liquid Whole Eggs Stored Under Abuse Refrigerated Conditions

2013 ◽  
Vol 2 (3) ◽  
pp. 65 ◽  
Author(s):  
Djamel Djenane ◽  
Javier Yangüela ◽  
Pedro Roncalés ◽  
Mohammed Aider
Keyword(s):  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Salmonella Enteritidis ◽  
Steam Distillation ◽  
Antibacterial Properties ◽  
Natural Food ◽  
Lavandula Angustifolia ◽  
Food Preservatives ◽  
Satureja Hortensis ◽  
Primary Compounds

<p>The steam distillation-extracted essential oils (EOs) of three aromatic plants from the Kabylie region of Algeria (<em>Eucalyptus globulus</em>, <em>Lavandula angustifolia</em>, and <em>Satureja hortensis</em>) were analyzed by gas chromatography coupled with mass spectrometry (GC/MS). The primary compounds from these EOs were 1,8-cineole (81.70%) for <em>Eucalyptus globulus</em>, 1,8-cineole (37.80%) and Beta-caryophyllene (20.90%) for <em>Lavandula angustifolia</em>, and carvacrol (46.10%), p-cymene (12.04%), and r-terpinene (11.43%) for <em>Satureja hortensis</em>. To test the antibacterial properties of the EOs, agar diffusion and microdilution methods were used for <em>Salmonella enterica</em> serovar Enteritidis CECT 4300. The results revealed that all of the EOs possessed a significant anti-Salmonella activity. The inhibition diameters for <em>Lavandula angustifolia</em> and Eucalyptus globulus were 41.30 and 35.26 mm, respectively, whereas the essential oil (EO) of <em>Satureja hortensis</em> showed a stronger anti-Salmonella activity (51.15 mm) when compared to the two other EOs. The minimum inhibitory concentration values ranged from 1 to 8 uL/mL, and the MIC value of the <em>Lavandula angustifolia</em> EO was the lowest (1 uL/mL). Moreover, the anti-Salmonella activity of the EOs added at various concentrations to liquid whole eggs was investigated, and the results showed that the antibacterial effect is proportional to the quantity of EO added to the product. Based on the observed anti-Salmonella activity, the EOs tested are promising natural alternatives for the preservation of liquid whole eggs stored at 7 ± 1ºC to simulate Algerian refrigeration conditions.</p>

scholarly journals Use of Antimicrobial and Genotoxicity Potentiality for Evaluation of Essential Oils as Food Preservatives

2009 ◽  
Vol 3 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Hend A. Hamedo ◽  
Hala M. Abdelmigid
Keyword(s):  
Essential Oils ◽  
Vicia Faba ◽  
Salmonella Enteritidis ◽  
Antimicrobial Agents ◽  
Chromosomal Abnormalities ◽  
Food Product ◽  
Natural Food ◽  
Food Preservatives ◽  
Plant Essential Oils ◽  
High Doses

In this study, investigations were carried out to assess the efficiency of two plant essential oils; rosemary and oregano as natural food preservatives. The effect of the plant essential oils at concentrations of 0.1%, 0.5% and 1% was studied in the soft cheese against Salmonella enteritidis and Escherichia coli at fridge temprature over a 14-day period. The essential oils performed well in the inhibition of S. enteritidis and E. coli. It is concluded that selected plant essential oils can act as potent inhibitors of both microorganisms in a food product. At the same time, evaluation of their safety as food preservatives was undertaken via monitoring the genotoxic activity of the mentioned essential oils using Vicia faba test. Vicia faba roots were treated with the above concentrations for 3 hours. Results revealed cytotoxic and genotoxic effects of the applied doses. Mitotic index decreased significantly when compared to control. Chromosomal abnormalities and micronuclei were also observed and the effects were dose-dependent. Despite the efficient role of the studied oils as antimicrobial agents, their genotoxicity potential in eukaryotic cells made them unacceptable as food preservatives, particularly at high doses. Therefore, more research in the use of essential oils as food preservatives is needed.

Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli O157:H7 and Staphylococcus aureus in minced beef

2011 ◽  
Vol 17 (6) ◽  
pp. 505-515 ◽  
Author(s):  
D. Djenane ◽  
J. Yangüela ◽  
T. Amrouche ◽  
S. Boubrit ◽  
N. Boussad ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Eucalyptus Globulus ◽  
Antibacterial Properties ◽  
Escherichia Coli O157 ◽  
Myrtus Communis ◽  
E Coli ◽  
Satureja Hortensis ◽  
Minced Beef

Essential oils (EOs) extracted by hydrodistillation from leaf parts of Algerian Eucalyptus globulus, Myrtus communis and Satureja hortensis were analyzed by gas chromatography/mass spectrometry (GC/MS). The main components of EOs obtained were γ-terpinene (94.48%), 1,8-cineole (46.98%) and carvacrol (46.10%), respectively, for E. globulus, M. communis and S. hortensis. The in vitro antimicrobial activity of the EOs was evaluated against Staphylococcus aureus CECT 4459 and Escherichia coli O157:H7 CECT 4267 using the agar diffusion technique. Results revealed that E. globulus and S. hortensis EOs had more antibacterial effects than that from M. communis. Minimal inhibitory concentrations (MIC) showed a range of 0.05–0.22% (volume by volume [v/v]). Sensitivity of gram-positive S. aureus was much higher than that of gram-negative E. coli. Plant EOs were added to minced beef (two-fold MIC value) at 0.10–0.44%, experimentally inoculated with the same pathogens at a level of 5 × 105 colony forming units (cfu)/g and stored at 5 ± 2 °C. Results showed that the EOs of E. globulus and S. hortensis had remarkable antibacterial properties, higher than that of M. communis, against S. aureus and E. coli. Indeed, a reduction of 5.8 log cfu/g (70.74% of reduction) was recorded after 7 days of storage for S. hortensis against E. coli. However, regarding S. aureus, both S. hortensis and E. globulus caused a highly significant ( p < 0.05) decrease of microbial counts, most evident after 5 days of storage; S. aureus numbers were 3.50 and 2.50 cfu/g, respectively, corresponding to a reduction of 2.20 and 3.20 log cfu/g (38.60 and 56.14% of reduction) after 1 week of storage. Sensory evaluation revealed that the aroma of minced beef meat treated with EOs was acceptable by panelists at the levels used.

scholarly journals Synergistic Antioxidant and Antibacterial Advantages of Essential Oils for Food Packaging Applications

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1267
Author(s):  
Nagaraj Basavegowda ◽  
Kwang-Hyun Baek
Keyword(s):  
Essential Oils ◽  
Shelf Life ◽  
Food Packaging ◽  
Chemical Constituents ◽  
Food Additives ◽  
Antibacterial Properties ◽  
Mechanisms Of Action ◽  
Life Extension ◽  
Natural Food ◽  
Food Preservatives

The development of food-borne and infectious diseases has increased globally at an anomalous rate and is combined with emerging social and economic problems. This highlights the need for new and improved antibacterial agents with novel and different mechanisms of action at regular intervals. Some chemical or artificial food additives are considered harmful if they are used beyond their permissible levels. Today, consumers are demanding alternative, green, safer, and natural food additives to increase the shelf life of food. Essential oils (EOs) are concentrated liquid mixtures of volatile compounds with antioxidant and antibacterial properties that can be used as natural, eco-friendly, renewable, and cost-effective additives. The use of combinations of different EOs and their components is a promising strategy to increase the synergistic and additive effects of EOs in foods. In this article, we review the recent literature on EOs concerning the chemical constituents, extraction methods, antioxidant and antibacterial activities, and their mechanisms of action. Additionally, we discuss the synergistic interaction of different EOs and their components, challenges, and future directions of EOs as natural food preservatives, with special emphasis on shelf life extension and applications in the packaging of food products.

scholarly journals Nanoencapsulation of Essential Oils as Natural Food Antimicrobial Agents: An Overview

2021 ◽  
Vol 11 (13) ◽  
pp. 5778
Author(s):  
Wei Liao ◽  
Waisudin Badri ◽  
Emilie Dumas ◽  
Sami Ghnimi ◽  
Abdelhamid Elaissari ◽  
...  
Keyword(s):  
Essential Oils ◽  
Food Systems ◽  
Food Industry ◽  
Antimicrobial Agents ◽  
Water Solubility ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Natural Food ◽  
Food Preservatives ◽  
Global Demand

The global demand for safe and healthy food with minimal synthetic preservatives is continuously increasing. Natural food antimicrobials and especially essential oils (EOs) possess strong antimicrobial activities that could play a remarkable role as a novel source of food preservatives. Despite the excellent efficacy of EOs, they have not been widely used in the food industry due to some major intrinsic barriers, such as low water solubility, bioavailability, volatility, and stability in food systems. Recent advances in nanotechnology have the potential to address these existing barriers in order to use EOs as preservatives in food systems at low doses. Thus, in this review, we explored the latest advances of using natural actives as antimicrobial agents and the different strategies for nanoencapsulation used for this purpose. The state of the art concerning the antibacterial properties of EOs will be summarized, and the main latest applications of nanoencapsulated antimicrobial agents in food systems will be presented. This review should help researchers to better choose the most suitable encapsulation techniques and materials.

scholarly journals Antimicrobial effects of spices and herbs essential oils

2009 ◽  
pp. 195-209 ◽  
Author(s):  
Marija Skrinjar ◽  
Nevena Nemet
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Food Additives ◽  
Inhibitory Effect ◽  
Natural Food ◽  
Garlic Mustard ◽  
Salmonella Spp ◽  
Food Preservatives ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp

Spices and herbs have been used as food additives since ancient times, as flavouring agents but also as natural food preservatives. A number of spices shows antimicrobial activity against different types of microorganisms. This article gives a literature review of recent investigations considering antimicrobial activity of essential oils widely used spices and herbs, such as garlic, mustard, cinnamon, cumin, clove, bay, thyme, basil, oregano, pepper, ginger, sage, rosemary etc., against most common bacteria and fungi that contaminate food (Listeria spp., Staphylococcus spp., Salmonella spp., Escherichia spp., Pseudomonas spp., Aspergillus spp., Cladosporium spp. and many others). Antimicrobial activity depends on the type of spice or herb, type of food and microorganism, as well as on the chemical composition and content of extracts and essential oils. Summarizing results of different investigations, relative antimicrobial effectiveness can be made, and it shows that cinnamon, cloves and mustrad have very strong antimicrobial potential, cumin, oregano, sage, thyme and rosemary show medium inhibitory effect, and spices such as pepper and ginger have weak inhibitory effect.

scholarly journals The Chemical Composition and Antibacterial Activities of the Essential Oils from Three Aframomum Species from Cameroon, and Their Potential as Sources of (E)-(R)-Nerolidol

2013 ◽  
Vol 8 (6) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Sylvie Kwanga Nguikwie ◽  
Maximilienne A. Nyegue ◽  
Florentine Ndoye-Foe Belinga ◽  
Rosalie A. Ngono Ngane ◽  
Bernard Romestand ◽  
...  
Keyword(s):  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antibacterial Activities ◽  
Natural Food ◽  
Seed Oils ◽  
Caryophyllene Oxide ◽  
Traditional Use ◽  
Food Preservatives ◽  
E Coli ◽  
Pure Compounds

Essential oils obtained by hydrodistillation of seeds, pericarps, leaves and rhizomes of Aframomum dalzielii, A letestuianum and A. pruinosum grown in Cameroon were analyzed by GC-FID and GC-MS. The seed oils of the three species were characterized by a high content of ( E)-( R)-nerolidol (>88.0 %), which was fully characterized by NMR spectroscopy and chiral GC analysis. The main constituents of the pericarp and rhizome oils were monoterpene hydrocarbons, mainly β-pinene (0.8%–22.9%) and sabinene (29.0%–42.3%), along with 1,8-cineole (4.5%–23.7%); leaf oils were characterized by sesquiterpenes, namely ( E)-β-caryophyllene (18.4%–82.4%) and caryophyllene oxide (4.5%–23.7%). The antibacterial activities of these essential oils and of nine pure compounds (sabinene, β-pinene, 1,8-cineole, linalool, racemic ( E)-nerolidol, ( E)-( R)-nerolidol, ( E)-β-caryophyllene, α-humulene and caryophyllene oxide) were assessed against Micrococcus luteus and Escherichia coli. The strongest activities were observed against E. coli. The seed essential oils and their major component, ( E)-( R)-nerolidol, exhibited the lowest MIC values (0.19-0.39 μL/mL), justifying their traditional use and their potential application as natural food preservatives.

scholarly journals Testing the antimicrobial activity of essential oils

2011 ◽  
pp. 71-74
Author(s):  
Adela Frankova ◽  
Pavel Kloucek ◽  
Jakub Smid ◽  
Lenka Nedorostova
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Vapor Phase ◽  
Salmonella Enteritidis ◽  
Food Preservatives ◽  
Fast Screening ◽  
Wide Range ◽  
In Vivo Testing

The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.

Effect of Some Essential Oils as Natural Food Preservatives on Commercial Grated Carrots

2010 ◽  
Vol 22 (3) ◽  
pp. 283-287 ◽  
Author(s):  
Flora V. Romeo ◽  
Serena De Luca ◽  
Amalia Piscopo ◽  
Emanuela De Salvo ◽  
Marco Poiana
Keyword(s):  
Essential Oils ◽  
Natural Food ◽  
Food Preservatives

Comparative Study on the Antibacterial Properties of Nanoemulsion of Zataria multiflora Essential Oil Fortified with Cinnamaldehyde Against Common Foodborne Pathogens

2020 ◽  
Vol 10 (5) ◽  
pp. 631-641
Author(s):  
Shahnaz Soufi ◽  
Majid Aminzare ◽  
Hassan Hassanzad Azar ◽  
Koorosh Kamali
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Foodborne Pathogens ◽  
Salmonella Enteritidis ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Suitable Alternative ◽  
Zataria Multiflora

Background: Nowadays, the application of several and natural preservatives in small quantity is a more preferred approach. In this regard, one of the effective methods is the formation of nanoemulsion of essential oils. Objective: The objective of this study was to compare the in vitro antibacterial activities of cinnamaldehyde (CIN) and Zataria multiflora essential oil in conventional (ZEO), nanoemulsion (NZEO) and fortified nanoemulsion (NZEOC) forms against common foodborne pathogens. Methods: Firstly, the Zataria multiflora essential oil was analyzed by GC-MS. The nanoemulsion of Z. multiflora essential oil was then prepared alone and fortified with cinnamaldehyde. Finally, their antimicrobial activity against Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli was evaluated. Results: Based on the results, carvacrol (36.62%) was found to be the most important compound of essential oil. In disc diffusion and micro-dilution methods, the addition of CIN to ZEO during nanoemulsion formation (NZEOC) showed more antibacterial activity when compared to the individual addition of NZEO and CIN (NZEO+CIN). However, according to the vapor phase diffusion method, nano-treatments exhibited less inhibitory effects than the other treatments. Conclusion: It can be concluded that the fortification of essential oils with their derived pure compounds during nanoemulsion formation, can be used as a suitable alternative to chemical antibacterial compounds in the food industry.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Two Aromatic Plants Cultivated in Morocco (Cinnamomum cassia and Origanum compactum)

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Asmae Chahbi ◽  
Saâdiya Nassik ◽  
Hamid El Amri ◽  
Ahmed Douaik ◽  
El Haj El Maadoudi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Antibacterial Properties ◽  
Biological Study ◽  
Synergistic Activity ◽  
Cinnamomum Cassia ◽  
Origanum Compactum

The present study aims to evaluate the antibacterial properties of natural products according to a pharmacodynamic approach in order to propose them as alternatives to synthetic products. Two essential oils (Cinnamomum cassia and Origanum compactum) were the subject of the chemical and biological study. First, we evaluated the sensitivity of the strains of avian Salmonella to the main antibiotics used and then to the chromatographic analysis of the composition of the two essential oils (EO); finally, we proceeded to the in vitro evaluation of the antibacterial activities of these EO (alone and in combination with antibiotics). The results obtained showed that carvacrol (35.2%), followed by γ-terpinene (20.1%), was the main constituent of the essential oil of O. compactum while cinnamaldehyde (69.1%) represents the major component of the essential oil of C. cassia. The antibioresistance profile of the Salmonella tested showed resistance to ampicillin (35%) and oxytetracycline (41.3%). Active products extracted from the essential oils studied showed antibacterial activity against Salmonella strains. C. cassia products were shown to be more active for Salmonella enteritidis (average inhibition diameter: 16.3 mm) and for Salmonella gallinarum (average inhibition diameter: 27.7 mm). The best synergistic activity with antibiotics has been obtained with the essential oil of C. cassia and its active product cinnamaldehyde. The minimum inhibitory concentration (MIC) of cinnamaldehyde is the lowest (0.05%). The results prove the presence of an antibacterial activity and a synergistic effect of two essential oils studied with the main antibiotics.

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