scholarly journals Evaluation of the Composition of Bioactive Compounds and Antioxidant Activity in Fourteen Apricot Varieties of North India

2017 ◽  
Vol 9 (5) ◽  
pp. 66 ◽  
Author(s):  
Sajad Mohd Wani ◽  
Peerzada Rashid Hussain ◽  
Farooq Ahmad Masoodi ◽  
Mukhtar Ahmad ◽  
Touseef Ahmad Wani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Radical Scavenging ◽  
Sinapic Acid ◽  
North India ◽  
Dpph Assay ◽  
Total Carotenoids ◽  
First Time

Variation in the content of bioactive compounds and antioxidant activity was evaluated in fourteen apricot varieties grown in north India. In the present study, the bioactive composition of apricot varieties of north Indian Kashmir was assessed by HPLC-MS/MS and their contribution towards free radical scavenging was assessed using DPPH, FRAP and ABTS assays. Studies revealed that content of bioactive compounds in apricot varieties was found to vary significantly (p ≤ 0.05) among cultivars. Content of total phenols was significantly (p ≤ 0.05) higher in Rakausilk variety (72.5±3.12 mg/100 g GAE) followed by Viva-gold (71.2±2.11 mg/100 g GAE) and Rakhchekarpo (69.6±1.65 mg/100 g GAE). Halman apricots exhibited the highest content of total carotenoids (12.2±2.13 mg/100 g) followed by Nugget (10.5±1.11 mg/100 g). HPLC analysis of apricot samples revealed the presence of fifteen phenolic acids in Halman, Venatchaa, Rakausilk and New Castle varieties; nine phenolic acid in Rakchekarpo and Sterling apricots; twelve phenolic acids in Shakanda, Nugget, Shakarpara and CITH-1 varieties; fourteen phenolic acid in Viva gold and Turkey varieties; seven phenolic acid in Khante and nineteen phenolic acids in CITH-2 apricots. Strong correlation existed between total phenolics and antioxidant activity for DPPH assay (r = 0.91) and ABTS radical inhibition (r = 0.86) while as moderate correlation existed for FRAP (r = 0.76). For DPPH and FRAP assays; EC50 values among apricot varieties were significantly (p ≤ 0.05) lower in Rakausilk (69.2, 123.6 mg/ml) followed by Viva Gold (74.3, 129.5 mg/ml) and Rakhchekarpo (77.4, 132.4 mg/ml). For ABTS.+ inhibition the EC50 values among apricot varieties were in the order of EC50(Rakausilk) < EC50(Khante) < EC50(Viva Gold). The present study reported the first time presence of p-coumaric acid-O-hexoside, isorhamnetin-O-glucouronide, Kaempferol-3-O-acetylhexoside, quercetin-3-O-rhamnoside, sinapic acid-O- hexoside, apigenin-7-O-glucoside, vitexin and luteolin-7-O-rutinoside compounds in apricot varieties of north India.

The Use of TLC-DPPH• Test with Image Processing to Study Direct Antioxidant Activity of Phenolic Acid Fractions of Selected Lamiaceae Family Species

2013 ◽  
Vol 96 (6) ◽  
pp. 1228-1232 ◽  
Author(s):  
Łukasz Cieśla ◽  
Dorota Staszek ◽  
Teresa Kowalska ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Image Processing ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Acidic Hydrolysis ◽  
Processing Program ◽  
Basic Hydrolysis ◽  
First Time

Abstract TLC coupled with 2,2-diphenyl-1-picrylhydrazyl staining was used to analyze phenolic acid fractions of selected Salvia and Thymus species. Documented videoscans were processed by means of an image processing program. This is the first time that free phenolic acids fractions, as well as fractions containing phenolic acids derived from basic and acidic hydrolysis, have been analyzed and compared for selected sage and thyme species. The analyzed samples along with caffeic acid (CA; standard) were chromatographed on silica gel plates with toluene – ethyl acetate – formic acid (60 + 40 + 1, v/v/v) mobile phase. The extracts were investigated with respect to the activity of CA. It was found that CA was most abundant in the fractions derived from basic hydrolysis. This compound was not detected in any of the fractions obtained after acidic hydrolysis. S. officinalis and S. triloba have similar free radical scavenging activity fingerprints obtained for all the analyzed fractions.

scholarly journals Biological activity of peel and pulp of Pseudocydonia sinensis Schneid. fruits

2019 ◽  
pp. 22-28
Author(s):  
O. V. Grygorieva ◽  
S. V. Klymenko ◽  
O. N. Vergun ◽  
J. Brindza ◽  
E. Ivanišová
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Acid ◽  
Radical Scavenging ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Antioxidant Power ◽  
Trolox Equivalent ◽  
Chinese Quince ◽  
Positive Correlations

Pseudocydonia sinensisSchneid. (Chinese quince) fruits are an excellent source of bioactive compounds. The objective of this study was to evaluate the antioxidant activity, total polyphenol, flavonoid, and phenolic acid content of dryPseudocydonia sinensisfruits (peel and pulp) of collection fromM.M.GryshkoNationalBotanical Garden (Kyiv,Ukraine). All tested samples (peel and pulp) exhibited DPPH radical scavenging activities with values from 9.31 to 10.23 and from 5.39 to 6.85 mg TEAC/g (trolox equivalent), respectively. Antioxidant activity determined by the molybdenum reducing antioxidant power method was ranged from 122.0 to 219.31 (peel) and from 62.19 to 95.44 (pulp) mg TEAC/g of dry matter (DM). The total polyphenol content of peel and pulp was ranged from 53.17 to 78.67 and from 32.31 to 47.41 mg GAE/g (gallic acid equivalent), respectively. The total flavonoid content was ranged from 9.15 to 26.18 (peel) and from 0.55 to 0.87 mg QE/g (pulp) (equivalent to quercetin). The phenolic acids content of peel and pulp fruits was ranged from 4.40 to 8.39 and from 1.12 to 3.97 mg CAE/g (caffeic acid equivalent), respectively. The results showed that peel extracts had the highest content of bioactive compounds compared with pulp extracts. Differences between the genotypes were significant in all observed parameters. The polyphenol content presents high positive correlations between antioxidant activity and phenolic content, flavonoid, and phenolic acid. This species is potential for propagation and practice use inUkraine.

scholarly journals Functional Properties and Antioxidant Activity of Morus alba L. Leaves var. Zolwinska Wielkolistna (WML-P)—The Effect of Controlled Conditioning Process

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 668
Author(s):  
Monika Przeor ◽  
Ewa Flaczyk ◽  
Dominik Kmiecik ◽  
Maciej S. Buchowski ◽  
Halina Staniek ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Total Phenolics ◽  
Radical Scavenging ◽  
Morus Alba ◽  
Scavenging Capacity ◽  
Fiber Fractions ◽  
High Antioxidant Activity ◽  
Traditional Processing

This study evaluated the effect of adding a new step, termed conditioning, to the traditional processing of leaves from Morus alba var. zolwinska wielkolistna grown in Poland (WML-P). This step, modeled on tea leaves processing, was conducted in a controlled environment on a semi-technical scale. The primary goal was to evaluate the effect of the WML-P conditioning for 1–4 h at 32–35 °C on the content of bioactive compounds (total phenolics, phenolic acids, flavonols, 1-deoxynojirimycin) and antioxidant activity (radical scavenging against DPPH, antioxidant capacity, chelating activity and ferric reducing antioxidant potential) of the lyophilized extracts. For the first time WML-P extracts content was comprehensively characterized by assessing dietary fiber fractions, fatty acids, amino acids, macro- and microelements and chlorophyll content. Compared to the traditional process, adding the conditioning step to WML-P processing resulted in an increased total phenolics content, radical scavenging capacity, ability to quench 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and iron-chelating ability in the lyophilized extracts. The beneficial effect depended on conditioning time. The highest flavonols and phenolic acids content were found after 2-h conditioning. We concluded that adding a 2-h conditioning step to traditional WML-P processing results in getting WML-P lyophilized extract with increased bioactive compounds content and high antioxidant activity.

scholarly journals Assessing Polyphenol Components and Antioxidant Activity during Fermented Assam Tea Ball Processing

2020 ◽  
Vol 12 (14) ◽  
pp. 5853
Author(s):  
Pimpinan Somsong ◽  
Chalat Santivarangkna ◽  
Pimsiri Tiyayon ◽  
Chi-Ming Hsieh ◽  
Warangkana Srichamnong
Keyword(s):  
Antioxidant Activity ◽  
Caffeic Acid ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Anaerobic Fermentation ◽  
Sinapic Acid ◽  
Total Phenolic ◽  
Tea Leaves ◽  
Antioxidant Power ◽  
Fermentation Time

Fermented tea is traditionally consumed in many Asian countries. In Thailand, the product is made by anaerobic submerged fermentation of semi-mature tea leaves before being made into a ball form. This study aims to investigate the composition of health-associated bioactive compounds in fermented tea balls made from Camellia sinensis var. assamica, which is naturally grown in the forests of northern Thailand. The processing involves steaming semi-mature tea leaves followed by anaerobic fermentation in 2% NaCl solution (1:5 w/v of tea leaves solution). Levels of catechin (C), epicatechin (EC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG), gallocatechin (GC), flavonols (myricetin, quercetin, and kaempferol), phenolic acids (caffeic acid, chlorogenic acid, coumaric acid, and sinapic acid), total phenolic content, and in vitro antioxidant activity were evaluated in fresh tea leaves, steamed tea leaves, and fermented tea leaves over a period of 60 days’ monitoring. The results indicated that fermented tea balls still contain significant amounts of tea polyphenols, although their processing may result in some loss of most bioactive compounds. The antioxidant activity measured by Ferric Reducing Antioxidant Power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity (ORAC) assays also declined as the fermentation time was extended. However, phenolic acids, including caffeic acid and sinapic acid, contrastingly increased during prolonged fermentation by 74.35% and 171.43% from fresh leaves, respectively.

Comparison of Bioactive Compounds of Matricaria recutita Extracted by Ultrasound and Maceration and their Effects on Preventing Sunflower Oil During Frying

2019 ◽  
Vol 15 (2) ◽  
pp. 156-164 ◽  
Author(s):  
Reza Farahmandfar ◽  
Maryam Asnaashari ◽  
Yegane Asadi ◽  
Batool Beyranvand
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Sunflower Oil ◽  
Radical Scavenging ◽  
Stability Index ◽  
Acid Value ◽  
Extraction Methods ◽  
Oil Stability Index ◽  
Matricaria Recutita

Background: It is important to study about the use of natural antioxidants as alternatives to synthetic ones due to the possibility of carcinogenic effects of synthetic antioxidants. This study is comparing the effect of the ultrasound-assisted and maceration extraction methods on antioxidant activity of Matricaria recutita. Methods: Bioactive compounds including phenolic, tocopherol, flavonoid and tannins and antioxidant activity of the extracts were evaluated. Moreover, extracts obtained from ultrasound and maceration methods were added to sunflower oil without any antioxidants at level of 200, 500 and 800 ppm, after that samples were heated at 180°C. Oxidation of the samples were evaluated after 0, 4, 8, 12, 16, 20 and 24 hours by measuring Peroxide Value (PV), Conjugated Diene (CD), Iodine Value (IV), Carbonyl Value (CV), Total Polar Compounds (TPC), Oil Stability Index (OSI), Color Index (CI) and acid value (AV). Results: The result showed total phenol (42.90 mg gallic acid/g extract), tocopherol (120.46 &#181;g &#945; - tocopherol/ml extract), flavonoid (2.64 mg/100 g extract) and tannins (3.89 mg gallic acid/g extract) of ultrasound extracts were higher than maceration extracts. Antioxidant activity of the extract was evaluated by DPPH assay which indicated 800 ppm of the Matricaria recutita extracted by ultrasound was the highest radical scavenging ability. Conclusion: Result indicated both ultrasound and maceration extracts could increase the oil oxidative stability but could not increase compared to BHA. In most cases, the extract samples by ultrasound had a better effect on stabilizing of sunflower oil during frying.

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals A Novel Stoichio-Kinetic Model for the DPPH• Assay: The Importance of the Side Reaction and Application to Complex Mixtures

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1019
Author(s):  
Lucrezia Angeli ◽  
Sebastian Imperiale ◽  
Yubin Ding ◽  
Matteo Scampicchio ◽  
Ksenia Morozova
Keyword(s):  
Antioxidant Activity ◽  
Kinetic Model ◽  
Rate Constant ◽  
High Resolution Mass Spectrometry ◽  
Sinapic Acid ◽  
Side Reaction ◽  
Main Reaction ◽  
Dpph Assay ◽  
Best Fitting ◽  
The Common

The 2,2-diphenyl-1-picrylhydrazyl (DPPH•) assay is widely used to determine the antioxidant activity of food products and extracts. However, the common DPPH• protocol uses a two-point measurement and does not give information about the kinetics of the reaction. A novel stoichio-kinetic model applied in this study monitors the consumption of DPPH• by common antioxidants following the second order reaction. The fitting of such decay yields the rate constant k1, which describes the main reaction between antioxidants and DPPH•, and the rate constant k2, which is attributed to a slower side reaction considering the products generated between the transient radicals (AO•) and another molecule of DPPH•. The model was first applied to antioxidant standards. Sinapic acid, Trolox and ascorbic and chlorogenic acids did not show any side reaction. Instead gallic, ferulic and caffeic acids achieved the best fitting with k2. The products of the side reaction for these compounds were confirmed and identified with high-resolution mass spectrometry. Finally, the kinetic model was applied to evaluate the antioxidant activity of eight herbal extracts. This study suggests a new kinetic approach to standardize the common DPPH• assay for the determination of antioxidant activity.

scholarly journals Distribution of Phenolic Acids and Antioxidant Activities of Different Bran Fractions from Three Pigmented Wheat Varieties

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jinli Zhang ◽  
Yan Ding ◽  
Haizhou Dong ◽  
Hanxue Hou ◽  
Xiansheng Zhang
Keyword(s):  
Phenolic Acids ◽  
Wheat Bran ◽  
Phenolic Acid ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Wheat Varieties

Phenolic acid profiles and antioxidant activities of outer bran, coarse bran, and shorts from blue, black, and purple wheat were analyzed. Phenolic acids were mainly in the bound form in pigmented wheat bran fractions. Phenolic acid content decreased in the order of outer bran, coarse bran, and shorts for the three pigmented wheat varieties. HPLC analysis of phenolic extracts demonstrated that the bound form of phenolic acids contained more ferulic, isoferulic, and p-coumaric acids compared to their free counterparts. Among the three pigmented wheat varieties, the bran fractions from blue wheat contained higher bound phenolic acids than the other two pigmented wheat bran fractions, except for purple coarse bran. The blue wheat outer bran had the highest total bound phenolic acid of 3458.71 μg/g while the purple wheat shorts had the lowest of 1730.71 μg/g. The contribution of bound phenolic acids to the total phenolic content and antioxidant activity was significantly higher than that of free phenolic acids. Blue wheat bran fractions had the highest radical scavenging activity against DPPH∙ while those of purple wheat gained the highest ABTS∙+ scavenging activity. High correlations were observed between TPC and radical scavenging capacities for DPPH and ABTS (R2>0.85, P<0.05).

scholarly journals SYNTHESIS, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF 2-ARYL BENZOTHIAZOLE DERIVATIVES

2018 ◽  
Vol 10 (5) ◽  
pp. 3
Author(s):  
Shaista Amin ◽  
Amrita Parle
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Aromatic Aldehydes ◽  
Benzoic Acids ◽  
Dpph Assay ◽  
Abts Assay ◽  
Antioxidant Assay ◽  
Benzothiazole Derivatives ◽  
Comparable Activity ◽  
Layer Chromatography

Objective: To synthesize benzothiazole derivatives, characterize them by 1HNMR and ATIR techniques and evaluate for their antioxidant activity.Methods: In the present study 12 benzothiazole derivatives were synthesized by reacting 2-chloronitro benzene as the primary reactant with different aromatic aldehydes and benzoic acids. Reactions were monitored using thin layer chromatography technique, and the newly synthesized derivatives were characterized by ATIR and 1HNMR techniques. The antioxidant assay was performed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS method and 2,2-diphenyl-1-picrylhydrazyl or DPPH method.Results: The antioxidant activity was found to be better in ABTS assay than DPPH assay. The compounds showed comparable activity to ascorbic acid at 100µg/ml.Conclusion: It was found that the synthesized benzothiazole derivatives showed significant radical scavenging potential.

scholarly journals Ultrasonic Extraction of Bioactive Compounds From Green Soybean Pods and Application in Green Soybean Milk Antioxidants Fortification

2021 ◽  
Author(s):  
Noppol Leksawasdi ◽  
Siraphat Taesuwan ◽  
Trakul Prommajak ◽  
Charin Techapun ◽  
Rattanaporn Khonchaisri ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bioactive Compound ◽  
Natural Antioxidants ◽  
Dependent Manner ◽  
Ultrasound Technique ◽  
Antioxidant Power ◽  
Soybean Milk

Abstract Green soybean (Glycine max L.) pods (GSP) are agro-industrial waste from the production of frozen green soybean and milk. These pods contain natural antioxidants and various bioactive compounds that are still underutilized. Polyphenols and flavonoids in GSP were extracted by ultrasound techniques and used in antioxidant fortification of green soybean milk. The ultrasound extraction that yielded the highest total polyphenol content was 50% amplitude for 10 min, whereas maximum flavonoids content was obtained at 50% amplitude for 15 min. Radical scavenging activity assayed by 2,2-diphenyl-1-picryl-hydrazyl radical (DPPH) and ferric reducing antioxidant power (FRAP) methods did not differ significantly (p>0.05) between the two conditions. Response surface methodology was applied to analyze an optimum ultrasonic-assisted extraction (UAE) condition of these variables. The highest desirability was found to be 50% amplitude with extraction time of 12.5 min. Fortification of the GSP extracts (1-3% v/v) in green soybean milk resulted in higher levels of bioactive compounds and antioxidant activity in a dose-dependent manner. Procyanidins were found to be the main polyphenols in dried GSP which were present at the concentration of 104.1 ± 2.1 mg/g dry sample, respectively. Addition of GSP extracts obtained by using an ultrasound technique to green soybean milk increased its bioactive compound content especially procyanidins as well as its antioxidant activity.

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