scholarly journals Comparative Different DNA Isolation Protocols from Ziziphus spina-christi (L.) Leaves through RAPD and ISSR Markers

2016 ◽  
Vol 8 (6) ◽  
pp. 49
Author(s):  
Fatemeh Bina ◽  
Zabihollah Zamani ◽  
Vahideh Nazeri ◽  
Daryush Talei
Keyword(s):  
Dna Extraction ◽  
Genomic Analysis ◽  
Restriction Enzymes ◽  
Fruit Trees ◽  
Issr Markers ◽  
Plant Tissues ◽  
Extraction And Purification ◽  
High Level ◽  
Rapd And Issr

<p>Genomic analysis of plants relies on high quantity and quality of pure DNA. Extraction and purification of DNA from woody and medicinal plants, such as fruit trees present a great challenge due to accumulation of a large amount of co-purify with DNA, including polysaccharides, polyphenols and proteins. Therefore, it is necessary to optimize the extraction protocols to reduce these compounds to the lowest level. A study was conducted to compare six DNA extraction and precipitation methods for genomic analysis in<em> Ziziphus spina-christi</em> (L.) plant tissues. The results showed significant differences in DNA contents among the six methods. Quantity and quality of extracted genomic DNAs were compared by employing the spectrophotometer, Nano-Drop, agarose gel electrophoresis, digestion by restriction enzymes and polymerase chain reaction (PCR) methods and molecular marker such as RAPD and ISSR. The method of Vroh Bi et al., provided the best results (208.89 ng/μL) in terms of quantity and quality of DNA, and Doyle and Doyle method as second method for leaves sample were chosen. According to the results, the method of Bi et al. is recommended for DNA extraction from plant tissues having high level of polysaccharides and phenol compounds.</p>

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

Heart Rate Recovery Changes during Competition Period in High-Level Basketball Players

2018 ◽  
Vol 1 (80) ◽  
Author(s):  
Audrius Gocentas ◽  
Anatoli Landõr ◽  
Aleksandras Kriščiūnas
Keyword(s):  
Heart Rate ◽  
Heart Rate Recovery ◽  
Team Sports ◽  
Basketball Players ◽  
Coeffi Cients ◽  
Competition Period ◽  
Monitoring Training ◽  
Intense Training ◽  
High Level

Research background and hypothesis. Replete schedule of competitions and intense training are features of contemporary team sports. Athletes, especially the most involved ones, may not have enough time to recover. As a consequence, aggregated fatigue can manifest in some undesirable form and affect athlete’s performance and health.Research aim. The aim of this study was to evaluate the changes in heart rate recovery (HRR) and investigate possible relations with sport-specifi c measures of effi cacy in professional basketball players during competition season.Research methods. Eight male high-level basketball players (mean ± SD, body mass, 97.3 ± 11.33 kg; height 2.02 ± 0.067 m, and age 23 ± 3.12 years) were investigated. The same basketball specifi c exercise was replicated several times from September till April during the practice sessions in order to assess the personal trends of HRR. Heart rate monitoring was performed using POLAR TEAM SYSTEM. Investigated athletes were ranked retrospectively according to the total amount of minutes played and the coeffi cients of effi cacy. Research results. There were signifi cant differences in the trends of HRR between the investigated players. The most effective players showed decreasing trends of HRR in all cases of ranking.Discussion and conclusions. Research fi ndings have shown that the quality of heart rate recovery differs between basketball players of the same team and could be associated with sport-specifi c effi cacy and competition playing time.Keywords: adaptation, autonomic control, monitoring training.

About Innovative Content of Subject-Oriented Pedagogical Education

2019 ◽  
Vol 28 (10) ◽  
pp. 106-117
Author(s):  
R. M. Asadullin
Keyword(s):  
Teacher Education ◽  
Teacher Training ◽  
Educational Process ◽  
Professional Activity ◽  
Subject Knowledge ◽  
Technological Structure ◽  
State Pedagogical ◽  
High Level ◽  
Competencies Development

The continuous modernization of the education system makes the problems of the quality of teacher training increasingly relevant. Moreover, the measures taken to improve the system of teacher education are largely confined to the introduction of new organizational and managerial mechanisms and practically do not affect the internal content and technological structure of the teacher training process.Modern pedagogical universities are constantly looking for innovative models of training teachers that will be able to solve non-standard social and professional tasks. However, recent studies in this area do not fully take into account the nature of pedagogical activity and conditions of its formation. Thus, the need arises for a special study of the processes and means of updating the content and technologies of teacher training in order to control the level of students’ professional competencies development, as required by educational and professional standards. This means the creation of a special educational system in a pedagogical university, which can provide a harmonious and synchronous mastering by future specialists of both subject knowledge and methods of pedagogical activity.The article provides a theoretical study aimed at identifying key patterns of designing a new content for teacher education, the basis of which is the formation of a future teacher as a subject of his own professional activity. The author describes the experience of using a subject-oriented model of education, implemented at Bashkir State Pedagogical University n.a. M. Akmulla. The effectiveness of this model is confirmed by the high level of students’ mastery of designing methods and constructing the educational process, as well as their positive experience in the implementation of educational activities.

Analisis Soal Matematika Tipe Higher Order Thinking Skills (HOTS) Tingkat SMP

2020 ◽  
Vol 2 (2) ◽  
pp. 112-129
Author(s):  
Lelly Oktafiana ◽  
Iis Holisin ◽  
Himmatul Mursyidah
Keyword(s):  
Power Level ◽  
Thinking Skills ◽  
Higher Order Thinking Skills ◽  
National Examination ◽  
Class Viii ◽  
Valid Test ◽  
Grade Material ◽  
Data Collection Technique ◽  
High Level

This study aims to describe the quality of the 2018 Mathematics National Examination (UN) in the HOTS types at the junior high level in terms of the level of validity, reliability, problem differentiation power, level of difficulty and distractor. This type of research is a descriptive study. The research was conducted at SMP Muhammadiyah 4 Surabaya and SMP Negeri 13 Surabaya for students in class VIII. The data collection technique used is a test. The test was taken from the 2018 math UN questions in odd semester VIII grade material including HOTS type. The number of UN mathematics questions in 2018 in the odd semester VIII class material consisted of 12 questions with 25% including LOTS types and 75% including HOTS types. The results showed: (1) 100% valid test questions, (2) high question reliability, (3) good problem differentiation power, (4) the difficuly level of the question 77,77% categorized as moderate and 2 question 22,23% are categorized as difficult, (5) there are 2 questions with one of the answer options do not work.

EFFICIENCY OF USING GENODIAGNOSTIC TEST IN ASSESSMENT OF PERINATAL INFECTION OF YOUNG ANIMALS IN PREVENTION OF LEUKEMIA OF CATTLE

2020 ◽  
Vol 1 (4) ◽  
pp. 450-455
Author(s):  
N.G. Kozyreva ◽  
◽  
I.Yu. Abashin ◽  
L.A. Ivanova ◽  
◽  
...  
Keyword(s):  
Dairy Products ◽  
Preventive Measures ◽  
Leukemia Virus ◽  
Genomic Analysis ◽  
Positive Trend ◽  
Infection Frequency ◽  
Perinatal Infection ◽  
Serological Method ◽  
Drinking Milk

This article presents the results of determining the level of perinatal infection of calves with the bovine leukemia virus at the age from birth to colostrum intake and 1-1.5 months after birth when drinking milk from healthy individuals, excluding the alimentary transmission route, with an infection frequency of 5.4% in the surveyed farm. Improvement of molecular diagnostics based on genomic analysis in the scheme of complex antiepizootic/recreational measures for bovine leukemia has been carried out in order to improve the quality of dairy products. As part of the implementation and improvement of the scheme of preventive measures, a positive trend was found, expressed in a tendency to a 3.6-fold decrease in the frequency of detection of BLV infection of young animals. In the course of work, the efficiency of the use of a gene diagnostic test - multiplex PCR-RT – as a tool for the specific prevention of bovine leukemia of calves in the early stages of the disease was shown: the relative diagnostic sensitivity of the PCR-RT technique is at a level not lower than that of the serological method and differs in the side of overestimation by 3.8 times.

Quality Improvement of the DNA extracted by boiling method in Gram negative bacteria

2017 ◽  
Vol 6 (04) ◽  
pp. 5347 ◽  
Author(s):  
Omar B. Ahmed* ◽  
Anas S. Dablool
Keyword(s):  
Dna Extraction ◽  
Control Method ◽  
Extraction Procedure ◽  
Cost Effective ◽  
High Yield ◽  
Gram Negative Bacteria ◽  
Bacterial Dna ◽  
Gram Negative ◽  
E Coli

Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality of the DNA obtained for each one of those methods are variable. The study aimed to evaluate bacterial DNA extraction using conventional boiling method followed by alcohol precipitation. DNA extraction from Gram negative bacilli was extracted and precipitated using boiling method with further precipitation by ethanol. The extraction procedure performed using the boiling method resulted in high DNA yields for both E. coli and K. pneumoniae bacteria in (199.7 and 285.7μg/ml, respectively) which was close to control method (229.3 and 440.3μg/ml). It was concluded that after alcohol precipitation boiling procedure was easy, cost-effective, and applicable for high-yield quality of DNA in Gram-negative bacteria.

Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

2021 ◽  
Author(s):  
Warepam Amuchou Singh ◽  
Nandeibam Samarjit Singh ◽  
Elangbam Julia Devi ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi
Keyword(s):  
Issr Markers ◽  
Gene Pools ◽  
Musa Spp ◽  
Pcr Rflp ◽  
Rapd And Issr

scholarly journals Evaluation of Physiological and Quality Parameters of Green Asparagus Spears Subjected to Three Treatments against the Decline Syndrome

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 937
Author(s):  
Francisco Javier López-Moreno ◽  
Santiago Atero-Calvo ◽  
Eloy Navarro-León ◽  
Begoña Blasco ◽  
Teresa Soriano ◽  
...  
Keyword(s):  
Early Death ◽  
Amino Acid Profile ◽  
Quality Parameters ◽  
Asparagus Officinalis ◽  
Chicken Manure ◽  
Economic Losses ◽  
Whole Plants ◽  
High Level ◽  
Green Asparagus

Green asparagus (Asparagus officinalis L.) is a widely grown and consumed crop which provides high-level nutritional interest. In recent years, the decline syndrome in asparagus plantations has been rapidly augmenting. This syndrome causes the early death of whole plants, also negatively affecting the new replanting. Decline causes notable economic losses in the sector. The objective of this work was to verify the effect of different treatments against asparagus decline syndrome on the physiological parameters and nutritional quality of the spears. To meet the objective, four different treatments were applied to asparagus plots strongly affected by decline syndrome: (T1) untreated control soil, (T2) biofumigation with Brassica pellets, (T3) biofumigation with chicken manure pellets, and (T4) disinfestation of the soil with Dazomet. The cumulative yield and physiological and quality parameters of green asparagus spears were studied. Thus, malondialdehyde (MDA), photosynthetic pigments, glutathione (GSH), ascorbate (AsA), total phenols, flavonoids, anthocyanin, antioxidant test, mineral nutrients, and the amino acid profile were measured on asparagus spears. The results showed that the Brassica pellets and Dazomet treatments were the most effective against the damage caused by the decline syndrome. However, it would be necessary to monitor the evolution in the following years.

scholarly journals AI-based localization and classification of skin disease with erythema

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ha Min Son ◽  
Wooho Jeon ◽  
Jinhyun Kim ◽  
Chan Yeong Heo ◽  
Hye Jin Yoon ◽  
...  
Keyword(s):  
Neural Network ◽  
Skin Diseases ◽  
Classification Model ◽  
Screening Tests ◽  
Sensitivity Score ◽  
Common Skin ◽  
Novel Method ◽  
Improved Performance ◽  
High Level

AbstractAlthough computer-aided diagnosis (CAD) is used to improve the quality of diagnosis in various medical fields such as mammography and colonography, it is not used in dermatology, where noninvasive screening tests are performed only with the naked eye, and avoidable inaccuracies may exist. This study shows that CAD may also be a viable option in dermatology by presenting a novel method to sequentially combine accurate segmentation and classification models. Given an image of the skin, we decompose the image to normalize and extract high-level features. Using a neural network-based segmentation model to create a segmented map of the image, we then cluster sections of abnormal skin and pass this information to a classification model. We classify each cluster into different common skin diseases using another neural network model. Our segmentation model achieves better performance compared to previous studies, and also achieves a near-perfect sensitivity score in unfavorable conditions. Our classification model is more accurate than a baseline model trained without segmentation, while also being able to classify multiple diseases within a single image. This improved performance may be sufficient to use CAD in the field of dermatology.

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