scholarly journals Molecular Cloning and Phylogenetic Analysis of Two Plant-Parasitic Nematode 14-3-3 Genes

2011 ◽  
Vol 3 (4) ◽  
Author(s):  
Dandan Liu ◽  
Lijie Chen ◽  
Yuxi Duan
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Cloning ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Plant Parasitic

scholarly journals The role of Caenorhabditis elegans sex-determination homologs, Mi-sdc-1 and Mi-tra-1 in Meloidogyne incognita

2021 ◽  
Author(s):  
Anil Baniya ◽  
Soumi Joseph ◽  
Larry Duncan ◽  
William Crow ◽  
Tesfamariam Mengistu
Keyword(s):  
Caenorhabditis Elegans ◽  
Sex Determination ◽  
Meloidogyne Incognita ◽  
Egg Production ◽  
Target Genes ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Root Knot Nematode ◽  
Model Free ◽  
Plant Parasitic

AbstractSex determination is a key developmental event in all organisms. The pathway that regulates sexual fate has been well characterized at the molecular level in the model free-living nematode Caenorhabditis elegans. This study aims to gain a preliminary understanding of sex-determining pathways in a plant-parasitic nematode Meloidogyne incognita, and the extent to which the roles of the sex determination genes are conserved in a hermaphrodite species, C. elegans, and plant-parasitic nematode species, M. incognita. In this study, we targeted two sex-determining orthologues, sdc-1 and tra-1 from M. incognita using RNA interference (RNAi). RNAi was performed by soaking second-stage juveniles of M. incognita in a solution containing dsRNA of either Mi-tra-1or Mi-sdc-1 or both. To determine the effect of RNAi of the target genes, the juveniles treated with the dsRNA were inoculated onto a susceptible cultivar of cowpea grown in a nutrient pouch at 28 °C for 5 weeks. The development of the nematodes was analyzed at different time points during the growth period and compared to untreated controls. Our results showed that neither Mi-sdc-1 nor Mi-tra-1 have a significant role in regulating sexual fate in M. incognita. However, the silencing of Mi-sdc-1 significantly delayed maturity to adult females but did not affect egg production in mature females. In contrast, the downregulation of Mi-tra-1 transcript resulted in a significant reduction in egg production in both single and combinatorial RNAi-treated nematodes. Our results indicate that M. incognita may have adopted a divergent function for Mi-sdc-1 and Mi-tra-1distinct from Caenorhabditis spp. However, Mi-tra-1 might have an essential role in female fecundity in M. incognita and is a promising dsRNA target for root-knot nematode (RKN) management using host-delivered RNAi.

Effects of organic amendments on plant-parasitic nematode populations, root damage, and banana plant growth

2011 ◽  
Vol 47 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Perrine Tabarant ◽  
Cécile Villenave ◽  
Jean-Michel Risède ◽  
Jean Roger-Estrade ◽  
Marc Dorel
Keyword(s):  
Plant Growth ◽  
Parasitic Nematode ◽  
Organic Amendments ◽  
Plant Parasitic Nematode ◽  
Root Damage ◽  
Banana Plant ◽  
Plant Parasitic

Effect of Previous Rotation on Plant Parasitic Nematode Population in Peanut and Crop Yield

cftm ◽  
2017 ◽  
Vol 3 (1) ◽  
pp. cftm2016.12.0086 ◽  
Author(s):  
David L. Jordan ◽  
Tommy Corbett ◽  
Clyde Bogle ◽  
Barbara Shew ◽  
Rick Brandenburg ◽  
...  
Keyword(s):  
Crop Yield ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Nematode Population ◽  
Plant Parasitic

scholarly journals In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.

BIO-PROTOCOL ◽  
2018 ◽  
Vol 8 (6) ◽  
Author(s):  
Maëlle Jaouannet ◽  
Chinh-Nghia Nguyen ◽  
Michaël Quentin ◽  
Stéphanie Jaubert-Possamai ◽  
Marie-Noëlle Rosso ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Parasitic Nematode ◽  
Plant Parasitic Nematode ◽  
Meloidogyne Spp ◽  
Plant Parasitic
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