scholarly journals Ovum Pick Up: Cows Treated With Single Doses of Follicle Stimulating Hormone

2019 ◽  
Vol 11 (10) ◽  
pp. 231 ◽  
Author(s):  
Bárbara Letícia Marchi da Silva ◽  
Paulo Roberto Adona ◽  
Samuel Guemra ◽  
Paulo Sergio Monzani ◽  
Moysés dos Santos Miranda
Keyword(s):  
Single Dose ◽  
Follicle Stimulating Hormone ◽  
Oocyte Retrieval ◽  
Ovarian Follicles ◽  
In Vitro Production ◽  
Oocyte Recovery ◽  
The Mean ◽  
Vitro Production ◽  
Stimulating Hormone

Cows treated with single doses of follicle stimulating hormone (FSH) and ovum pick up (OPU) after 24 hours were evaluated for oocyte recovery, in vitro production of embryos (IVPE), and transferred embryos. To begin evaluations, the ovarian follicles larger than three millimeters in diameters were removed from all cows used in the study. Two days after OPU, 200 milligrams of FSH was given in a single dose in 6 cows (treated). Twenty-four hours after application of FSH, the cows underwent a new OPU session for oocyte retrieval. These procedures were repeated three consecutive times without interval. In control (FSH-free) cows the OPU were performed at intervals of one week or oocyte retrieval. The viable oocytes were submitted to IVPE, and the blastocysts were transferred to the recipients. The mean number of oocytes did not differ (p > 0.05) between control cows (12.1±2.8) and those treated (10.9±1.6). There were also no differences (p > 0.05) in the number (6.6±1.7 and 7.1±0.9, respectively) or in the percentage (54.4±3.3 and 64.5±3.1%, respectively) of viable oocytes between control cows and those treated. The percentage of IVPE and pregnancy did not differ (p > 0.05) between control cows (39.8±2.6% and 44.7±4.8%) and those treated (37.8±2.5% and 39.5±4.1%), respectively. The treatment with single doses of FSH did not significantly alter the evaluated results for oocyte recovery, IVPE and transferred embryos, and, therefore, did not promote significant improvements for the IVPE as a whole.

scholarly journals Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

2019 ◽  
Vol 56 (3) ◽  
pp. e156894 ◽  
Author(s):  
Mayara Ellen Bardi de Moraes ◽  
Paulo Roberto Adona ◽  
Samuel Guemra ◽  
Tiago Henrique Camara De Bem ◽  
Moysés Dos Santos Miranda
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Follicle Stimulating Hormone ◽  
Vitro Fertilization ◽  
Average Recovery ◽  
Follicular Aspiration ◽  
Oocyte Recovery ◽  
The Mean ◽  
Stimulating Hormone

The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

scholarly journals Oocyte recovery and in vitro embryo production in cows treated with a single dose of follicle-stimulating hormone

2020 ◽  
Vol 44 (1) ◽  
pp. 151-155
Author(s):  
Thiago Marquez FERNANDES ◽  
Paulo Roberto ADONA ◽  
Samuel GUEMRA ◽  
Tiago Henrique Camara DE BEM ◽  
Moysés dos Santos MIRANDA
Keyword(s):  
Single Dose ◽  
Follicle Stimulating Hormone ◽  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production ◽  
Stimulating Hormone

scholarly journals Effects of follicle-stimulating hormone and serum substitution on the in-vitro growth of human ovarian follicles

1999 ◽  
Vol 14 (6) ◽  
pp. 1555-1562 ◽  
Author(s):  
C.S. Wright ◽  
O. Hovatta ◽  
R. Margara ◽  
G. Trew ◽  
R.M.L. Winston ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
In Vitro Growth ◽  
Human Ovarian Follicles ◽  
Stimulating Hormone

221 EFFECT OF STAGE OF CORPUS LUTEUM DEVELOPMENT ON THE IN VITRO PRODUCTION OF BOVINE EMBRYOS

2011 ◽  
Vol 23 (1) ◽  
pp. 209
Author(s):  
S. Miyashita ◽  
K. Miyata ◽  
C. Tachibana ◽  
Y. Inaba ◽  
H. Koyama ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Calf Serum ◽  
Cumulus Cells ◽  
Large Mass ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Stage Of Development ◽  
The Mean ◽  
Vitro Production

The objective of this study was to investigate the effect of stage of corpus luteum (CL) development on the in vitro production of bovine embryos. Ovaries were classified according to the expected day of the oestrous cycle based on the morphology of the ovaries. Ovaries with a corpus hemorrhagicum and the remnant of the follicular lumen filled with blood were considered the early luteal stage (Days 2 to 4; Day 0 = day of ovulation, n = 46). Ovaries with a large mass of orange tissue in the CL were classified as the midluteal stage (Days 7 to 10, n = 42). Cumulus–oocyte complexes (COC) were collected by aspiration of 2- to 6-mm follicles. The COC were classified into the following grades: COC with >3 compact layers of cumulus cells and evenly granulated cytoplasm were classified into Grade 1; COC with >3 layers cumulus cells and evenly granulated cytoplasm were classified into Grade 2; COC with partially remaining cumulus cells and abnormal cytoplasm were classified into Grade 3; COC without cumulus cells or those with expanded cumulus cells were classified into Grades 4 and 5, respectively. Grades 1 and 2 COC were in vitro matured for 20 h in TCM-199 supplemented with 5% calf serum and 0.02 mg mL–1 of FSH at 38.5°C under an atmosphere of 5% CO2 in air. Matured COC were inseminated with 5 × 106 sperm for 18 h. Presumptive zygotes were cultured in CR1aa medium supplemented with 5% calf serum at 38.5°C under an atmosphere of 5% O2, 5% CO2, and 90% N2 for 9 days (fertilization = Day 0). The mean number of COC and the proportion of COC classified as Grades 1 and 2 were analysed by ANOVA. Cleavage rates on Day 3 and blastocyst rates on Days 7 to 9 were analysed by a chi-square test. The mean number of recovered oocytes in the early luteal stage (18.7 ± 9.5) was significantly higher (P < 0.05) than the number in the midluteal stage (12.2 ± 5.7). The proportion of Grades 1 and 2 oocytes in the early luteal stage [66.7% (531/789)] was significantly higher (P < 0.01) than that in the midluteal stage [51.6% (252/484)]. The cleavage and blastocyst rates in the early luteal stage [60.9% (181/297) and 32.7% (97/297), respectively] were significantly higher (P < 0.05) than those in the midluteal stage [50.7% (76/150) and 20.7% (31/150) respectively].The present study suggests that the stage of development of the CL in bovine ovaries influences the number of recovered oocytes per ovary and the development of in vitro production of bovine embryos.

scholarly journals Kinetic analysis of in vitro production of wild-type Spodoptera frugiperda nucleopolyhedrovirus

2010 ◽  
Vol 53 (2) ◽  
pp. 285-291 ◽  
Author(s):  
Andréa Farias de Almeida ◽  
Gorete Ribeiro de Macedo ◽  
Leslie Chee Loong Chan ◽  
Márcia Regina da Silva Pedrini
Keyword(s):  
Cell Line ◽  
Spodoptera Frugiperda ◽  
Wild Type ◽  
In Vitro Production ◽  
Extracellular Virus ◽  
Cell Productivity ◽  
The Mean ◽  
Body Production ◽  
Vitro Production

In this study, the kinetic behavior of Sf9 and Sf21 cells used in the production of a baculovirus biopesticide to control the pest of corn Spodoptera frugiperda was analyzed. Kinetic variables such as maximum specific growth rate, cell productivity, mean rate of infection, as well as the mean rate of occlusion body production were determined during the infection of these cell-lines with the extracellular virus of the S. frugiperda nucleopolyhedrovirus (SfMNPV). The Sf9 cell-line resulted in better viral production results (5.0 x 10(8) OB/mL) than the Sf21 cell-line (2.5 x 10(8) OB/mL).

EFFECT OF OVINE LUTEINIZING HORMONE ON THE SECRETION OF PROGESTERONE IN VITRO BY OVARIAN FOLLICLES FROM HYPOPHYSECTOMIZED RATS TREATED WITH FOLLICLE-STIMULATING HORMONE

1978 ◽  
Vol 77 (3) ◽  
pp. 419-420
Author(s):  
A. R. LABARBERA ◽  
MERO R. NOCENTI
Keyword(s):  
New York ◽  
Luteinizing Hormone ◽  
Corn Oil ◽  
Follicle Stimulating Hormone ◽  
Virgin Female ◽  
Ovarian Follicles ◽  
Female Rats ◽  
Hypophysectomized Rats ◽  
Stimulating Hormone

Department of Physiology, College of Physicians and Surgeons, Columbia University, New York 10032, U.S.A. (Received 14 November 1977) Ovarian follicles from oestrous, pro-oestrous and hypophysectomized rats have the capacity to secrete progestins in vitro and to respond to luteinizing hormone (LH) by increasing this secretion (Stoklosowa & Nalbandov, 1972; LaBarbera, Nocenti & Castellano, 1974; Lindner, Tsafriri, Lieberman, Zor, Koch, Bauminger & Barnea, 1974). Follicles from hypophysectomized rats, untreated or treated with follicle-stimulating hormone (FSH), were therefore studied in experiments in vitro to investigate further the gonadotrophic control of progesterone secretion. Mature virgin female rats (200–250 g) were hypophysectomized and, beginning on day 4 after the operation, received a total of 360 μg ovine FSH (NIH-FSH-S9)/100 g body weight, injected s.c. in corn oil as six divided doses, one every 12 h for 3 days. Untreated controls received corn oil only. On day 7 after hypophysectomy, the ovaries were excised and

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