scholarly journals Determination of the Prevalence of Chlamydia psittaci by PCR in Iranian Pigeons

2011 ◽  
Vol 3 (4) ◽  
Author(s):  
Abbas Doosti ◽  
Asghar Arshi
Keyword(s):  
Chlamydia Psittaci

scholarly journals Molecular detection of Chlamydia psittaci and Chlamydia felis in human keratoconjunctivitis cases

2020 ◽  
Vol 23 (1) ◽  
pp. 130-137
Author(s):  
S. Jazi ◽  
A. Mokhtari ◽  
A. Ebrahimi Kahrizsangi
Keyword(s):  
Veterinary Medicine ◽  
Significant Relationship ◽  
Laboratory Tests ◽  
Molecular Detection ◽  
Chlamydia Psittaci ◽  
Diagnostic Measures ◽  
High Incidence ◽  
Low Efficiency ◽  
Low Rate

Given the high incidence of keratoconjunctivitis in Iran (approximately 3.6–53.9%) and low efficiency of clinical diagnostic measures, application of laboratory tests for detection of different keratoconjunctivitis/conjunctivitis causes and determination of their accurate prevalence is essential. In this research, conjunctival samples were collected from 100 patients with keratoconjunctivitis signs referred to an eye hospital of Iran. After DNA extraction, PCR was carried out for detection of Chlamydia psittaci and Chlamydia felis. PCR positive products were further subjected for DNA sequencing. In this study, one sample was Chlamydia psittaci positive and none was positive for Chlamydia felis. There wasn’t a statistically significant relationship between working in the field of veterinary medicine or keeping a pet and Chlamydia psittaci prevalence (P>0.05). This study showed a low rate of chlamydial keratoconjunctivitis and therefore further studies for detection of other causes are necessary.

scholarly journals Chlamydiales order bacteria in swine with reproductive disoders. Detection of Waddlia chondrophila in swine

2019 ◽  
Vol 21 (95) ◽  
pp. 122-126
Author(s):  
V. K. Zezekalo
Keyword(s):  
Restriction Analysis ◽  
Mortality Rates ◽  
Chlamydia Psittaci ◽  
Chlamydia Infection ◽  
Young Stock ◽  
Chlamydia Pecorum ◽  
Pcr Product ◽  
Chlamydia Suis

Chlamydial infections cause significant economic expenses due to infertility, insufficient productivity growth, and high mortality rates among young stock. Bacteria of the Chlamydiaceae family (Chlamydia suis Chlamydia abortus, Chlamydia psittaci and Chlamydia pecorum) are well known pathogens isolated from pigs. The diseases associated with these species are widely studied; monitoring and prevention are carried out. However, chlamydia-related bacteria in swine remain poorly studied in Ukraine. The purpose of the study was to check samples, taken from swine from private farms in the Poltava region, Ukraine, for the presence of Chlamydia-like organisms. Determination of the possibility of coinfection of Chlamydia and Chlamydia-like organisms. We examined 28 samples from two private farms in the Poltava region (23 samples from sows, and 5 samples from boars). The studies were carried out using PCR with primers designed in our laboratory. As a result of the study, Chlamydiaceae-positive samples were not detected among sows. Along with it, 2 out of 5 samples taken from boars were found to be Chlamydiaceae-positive. The total number of Chlamydiaceae-positive samples was 2 out of 28 (7.14%). The total number of Waddlia-positive samples was 8 out of 28 (28.7%). A mixture of Chlamydiaceae and Waddlia chondrophila infection was found in one sample. Parachlamydia acanthamoebae was not found in any sample. There are no reports of the detection of Waddlia chondrophila in samples from swine in the sources available for us, so this publication might be the first such report. Due to the fact that W. chondrophila was first detected in the samples selected from pigs, the experiment was repeated three times, and the identity of the PCR product of W. chondrophila-positive samples was additionally verified by restriction analysis. Relatively small number of samples, in our research as well as in previous studies, does not allow us to make accurate calculations about the prevalence of chlamydia-like organisms in swine. Further research are required and might include broader sampling, isolation of the pathogen, sequencing of its genome, experimental infection, determination of pathomorphological changes. All the listed above, would help to determine significance of the study along with the role of W. chondrophila in chlamydia infection in swine.

scholarly journals Immune adherence hemagglutination: alternative to complement-fixation serology

1978 ◽  
Vol 7 (3) ◽  
pp. 282-285
Author(s):  
E T Lennette ◽  
D A Lennette
Keyword(s):  
Complement Fixation ◽  
Chlamydia Psittaci ◽  
Serological Testing ◽  
Type B ◽  
Immune Adherence ◽  
Standard Procedures ◽  
Human Sera ◽  
Increased Sensitivity ◽  
Time Required

Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.

Galactic orbits of stars

1966 ◽  
Vol 25 ◽  
pp. 93-97
Author(s):  
Richard Woolley
Keyword(s):  
Globular Cluster ◽  
Potential Field ◽  
High Velocity ◽  
Velocity Vector ◽  
Variable Stars ◽  
The Sun ◽  
Proper Motions ◽  
Rr Lyrae ◽  
The Galaxy

It is now possible to determine proper motions of high-velocity objects in such a way as to obtain with some accuracy the velocity vector relevant to the Sun. If a potential field of the Galaxy is assumed, one can compute an actual orbit. A determination of the velocity of the globular clusterωCentauri has recently been completed at Greenwich, and it is found that the orbit is strongly retrograde in the Galaxy. Similar calculations may be made, though with less certainty, in the case of RR Lyrae variable stars.

Distance to SN 1987A and the LMC

1999 ◽  
Vol 190 ◽  
pp. 549-554
Author(s):  
Nino Panagia
Keyword(s):  
Angular Size ◽  
Large Magellanic Cloud ◽  
Magellanic Cloud ◽  
Light Curves ◽  
Distance Modulus ◽  
Absolute Size ◽  
Sn 1987A

Using the new reductions of the IUE light curves by Sonneborn et al. (1997) and an extensive set of HST images of SN 1987A we have repeated and improved Panagia et al. (1991) analysis to obtain a better determination of the distance to the supernova. In this way we have derived an absolute size of the ringRabs= (6.23 ± 0.08) x 1017cm and an angular sizeR″ = 808 ± 17 mas, which give a distance to the supernovad(SN1987A) = 51.4 ± 1.2 kpc and a distance modulusm–M(SN1987A) = 18.55 ± 0.05. Allowing for a displacement of SN 1987A position relative to the LMC center, the distance to the barycenter of the Large Magellanic Cloud is also estimated to bed(LMC) = 52.0±1.3 kpc, which corresponds to a distance modulus ofm–M(LMC) = 18.58±0.05.

International determination of the total motion of the pole

1961 ◽  
Vol 13 ◽  
pp. 29-41
Author(s):  
Wm. Markowitz
Keyword(s):  
Secular Motion ◽  
The Future

A symposium on the future of the International Latitude Service (I. L. S.) is to be held in Helsinki in July 1960. My report for the symposium consists of two parts. Part I, denoded (Mk I) was published [1] earlier in 1960 under the title “Latitude and Longitude, and the Secular Motion of the Pole”. Part II is the present paper, denoded (Mk II).

Time and Latitude in South Africa

1972 ◽  
Vol 1 ◽  
pp. 27-38
Author(s):  
J. Hers
Keyword(s):  
South Africa ◽  
Cape Town ◽  
Astronomical Observations ◽  
Royal Observatory ◽  
The Republic ◽  
Astronomical Determination ◽  
Limited Accuracy ◽  
Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

Large-scale Motion of Solar Filaments

2000 ◽  
Vol 179 ◽  
pp. 205-208
Author(s):  
Pavel Ambrož ◽  
Alfred Schroll
Keyword(s):  
Magnetic Flux ◽  
Proper Motion ◽  
Time Scale ◽  
Large Scale ◽  
Accuracy Of Measurements ◽  
Solar Filaments ◽  
General Movement ◽  
Scale Motion ◽  
Velocity Scatter

AbstractPrecise measurements of heliographic position of solar filaments were used for determination of the proper motion of solar filaments on the time-scale of days. The filaments have a tendency to make a shaking or waving of the external structure and to make a general movement of whole filament body, coinciding with the transport of the magnetic flux in the photosphere. The velocity scatter of individual measured points is about one order higher than the accuracy of measurements.

Practical Realization of a Reference System for Earth Dynamics by Satellite Methods

1975 ◽  
Vol 26 ◽  
pp. 341-380 ◽  
Author(s):  
R. J. Anderle ◽  
M. C. Tanenbaum
Keyword(s):  
Reference Frame ◽  
Polar Motion ◽  
Pole Position ◽  
Control Points ◽  
Significant Information ◽  
Crustal Motion ◽  
Average Reference ◽  
Future Data ◽  
Existing Data

AbstractObservations of artificial earth satellites provide a means of establishing an.origin, orientation, scale and control points for a coordinate system. Neither existing data nor future data are likely to provide significant information on the .001 angle between the axis of angular momentum and axis of rotation. Existing data have provided data to about .01 accuracy on the pole position and to possibly a meter on the origin of the system and for control points. The longitude origin is essentially arbitrary. While these accuracies permit acquisition of useful data on tides and polar motion through dynamio analyses, they are inadequate for determination of crustal motion or significant improvement in polar motion. The limitations arise from gravity, drag and radiation forces on the satellites as well as from instrument errors. Improvements in laser equipment and the launch of the dense LAGEOS satellite in an orbit high enough to suppress significant gravity and drag errors will permit determination of crustal motion and more accurate, higher frequency, polar motion. However, the reference frame for the results is likely to be an average reference frame defined by the observing stations, resulting in significant corrections to be determined for effects of changes in station configuration and data losses.

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