scholarly journals Molecular Analysis of Polymorphisms in IL-6 Gene and the Predisposition to Osteoporosis Using Variable Number of Tandem Repeats-Polymerase Chain Reaction (VNTRS-PCR) Technique

2011 ◽  
Vol 3 (2) ◽  
Author(s):  
Maysaa Abdul Razzaq Dhahi ◽  
Shymaa Jamal Al-Anee ◽  
Sadeic Auaid. Ali ◽  
Ahmed Samir Al-Naaimi
Keyword(s):  
Polymerase Chain Reaction ◽  
Molecular Analysis ◽  
Tandem Repeats ◽  
Variable Number ◽  
Chain Reaction ◽  
Polymerase Chain

Tuberculosis in swine co-infected withMycobacterium aviumsubsp.hominissuisandMycobacterium bovisin a cluster from Argentina

2014 ◽  
Vol 143 (5) ◽  
pp. 966-974 ◽  
Author(s):  
S. BARANDIARAN ◽  
A. M. PÉREZ ◽  
A. K. GIOFFRÉ ◽  
M. MARTÍNEZ VIVOT ◽  
A. A. CATALDI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Null Hypothesis ◽  
Tandem Repeats ◽  
Spatial Clustering ◽  
Variable Number ◽  
Molecular Tools ◽  
Chain Reaction ◽  
Analysis Techniques ◽  
Swine Population ◽  
Polymerase Chain

SUMMARYIn Argentina little is known about the epidemiology of tuberculosis (TB) infection in swine. We characterized the epidemiological dynamics ofMycobacterium aviumcomplex (MAC) infection in a swine population of Argentina using molecular tools and spatial analysis techniques. Isolates (n = 196) obtained from TB-like lesions (n = 200) were characterized by polymerase chain reaction. The isolates were positive to eitherM. bovis(IS6110) (n = 160) orM. avium(IS1245) (n= 16) while the remaining 20 (10·2%) isolates were positive to bothM. bovisandM. avium. The detection of both bacteria together suggests co-infection at the animal level. In addition, MAC-positive isolates (n = 36) were classified asM. aviumsubsp.avium(MAA) (n = 30) andM. aviumsubsp.hominissuis(MAH) (n = 6), which resulted in five genotypes when they were typed using mycobacterial interspersed repetitive unit, variable number of tandem repeats (MIRU-VNTR). One significant (P = 0·017) spatial clustering of genotypes was detected, in which the proportion of MAH isolates was larger than expected under the null hypothesis of even distribution of genotypes. These results show that in Argentina the proportion of TB cases in pigs caused byM. aviumis larger than that reported in earlier studies. The proportion ofM. bovis–MAC co-infections was also higher than in previous reports. These results provide valuable information on the epidemiology of MAC infection in swine in Argentina.

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