scholarly journals Phosphorus Non-Point Pollution from Equestrian Wastes and the Need for Recycling

2021 ◽  
Vol 10 (2) ◽  
pp. 61
Author(s):  
J. William Louda ◽  
Bobby G. Duersch ◽  
Jeffrey T. Osetek ◽  
Charmaine Cintron ◽  
Lorraine Chaljub ◽  
...  
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Organic Phosphorus ◽  
Dry Weight ◽  
Pollution Source ◽  
South Florida ◽  
Horse Manure ◽  
Pasture Grasses ◽  
Wet Weight ◽  
Extractable Phosphorus

South Florida and much of the rest of the World suffers from harmful algal blooms (HABs) and controls of both nitrogen (N) and phosphorus (P) pollution are required to curtail the onset, spread and/or expansion of these blooms. This report covers our studies on several aspects of equestrian waste (viz. horse manure) aimed at yielding an overview of phosphorus and its pollution stemming from non-point horse manure sources in portions of Palm Beach County Florida. Methods included a modified Hedley extraction sequence, emphasizing ‘easily extractable phosphorus’ (EEP), and 31P nuclear magnetic resonance (NMR) spectroscopic identification of organic phosphorus (Po) species. Samples included fresh and aged horse manure, pasture soils, horse feed and pasture grasses, and canal waters adjacent to equestrian or agricultural fields. Easily extractable Phosphorus (EEP) averaged about 54-77% of the total horse manure phosphorus. Total phosphorus ranged from 13,020 – 22,300 mg per kilogram dry weight. (≈60-100 lbs. P2O5 / ton and on a wet weight basis, this equates to 4,000 to 14,818 grams-P/ U.S. ton or 8.8 to 32.6 pounds of phosphorus (≈ 20-75 lb. P2O5) per wet weight ton of horse manure. Considering the values of EEP in fresh samples from a single horse, we found a range of 8,000 – 17,000 mg-P/kg (8-17 g-P/kg) dry weight horse manure. Soil samples yielded the highest P in the NaOH extract of the Hedley sequence. This equates to the Al, Fe and ester forms. Phosphorus (viz. EEP) runoff is viewed here as a non-point P pollution source.

scholarly journals Understanding the Differences in the Growth and Toxin Production of Anatoxin-Producing Cuspidothrix issatschenkoi Cultured with Inorganic and Organic N Sources from a New Perspective: Carbon/Nitrogen Metabolic Balance

Toxins ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 724
Author(s):  
Siyi Tao ◽  
Suqin Wang ◽  
Lirong Song ◽  
Nanqin Gan
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
Toxin Production ◽  
Organic N ◽  
Control Group ◽  
Metabolic Balance ◽  
N Sources ◽  
Inorganic And Organic

Cyanotoxins are the underlying cause of the threat that globally pervasive Cyanobacteria Harmful algal blooms (CyanoHABs) pose to humans. Major attention has been focused on the cyanobacterial hepatotoxin microcystins (MCs); however, there is a dearth of studies on cyanobacterial neurotoxin anatoxins. In this study, we explored how an anatoxin-producing Cuspidothrix issatschenkoi strain responded to culture with inorganic and organic nitrogen sources in terms of growth and anatoxins production. The results of our study revealed that ʟ- alanine could greatly boost cell growth, and was associated with the highest cell productivity, while urea significantly stimulated anatoxin production with the maximum anatoxin yield reaching 25.86 μg/mg dry weight, which was 1.56-fold higher than that in the control group (BG11). To further understand whether the carbon/nitrogen balance in C. issatschenkoi would affect anatoxin production, we explored growth and toxin production in response to different carbon/nitrogen ratios (C/N). Anatoxin production was mildly promoted when the C/N ratio was within low range, and significantly inhibited when the C/N ratio was within high range, showing approximately a three-fold difference. Furthermore, the transcriptional profile revealed that anaC gene expression was significantly up-regulated over 2–24 h when the C/N ratio was increased, and was significantly down-regulated after 96 h. Overall, our results further enriched the evidence that urea can stimulate cyanotoxin production, and ʟ-alanine could boost C. issatschenkoi proliferation, thus providing information for better management of aquatic systems. Moreover, by focusing on the intracellular C/N metabolic balance, this study explained the anatoxin production dynamics in C. issatschenkoi in response to different N sources.

scholarly journals Integrated observing systems: An approach to studying harmful algal blooms in south Florida

2019 ◽  
Vol 12 (sup2) ◽  
pp. S187-S198 ◽  
Author(s):  
Adam M. Schaefer ◽  
M. Dennis Hanisak ◽  
Malcolm McFarland ◽  
James M. Sullivan
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
South Florida ◽  
Observing Systems

scholarly journals Distribution of Harmful Algal Growth-Limiting Bacteria on Artificially Introduced Ulva and Natural Macroalgal Beds

2020 ◽  
Vol 10 (16) ◽  
pp. 5658
Author(s):  
Nobuharu Inaba ◽  
Isamu Kodama ◽  
Satoshi Nagai ◽  
Tomotaka Shiraishi ◽  
Kohei Matsuno ◽  
...  
Keyword(s):  
Biological Control ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Algal Growth ◽  
Macroalgal Species ◽  
Chattonella Antiqua ◽  
Cultured Fish ◽  
Wet Weight ◽  
Artificial Introduction ◽  
Hab Species

The intensity and frequency of harmful algal blooms (HABs) have increased, posing a threat to human seafood resources due to massive kills of cultured fish and toxin contamination of bivalves. In recent years, bacteria that inhibit the growth of HAB species were found to be densely populated on the biofilms of some macroalgal species, indicating the possible biological control of HABs by the artificial introduction of macroalgal beds. In this study, an artificially created Ulva pertusa bed using mobile floating cages and a natural macroalgal bed were studied to elucidate the distribution of algal growth-limiting bacteria (GLB). The density of GLB affecting fish-killing raphidophyte Chattonella antiqua, and two harmful dinoflagellates, were detected between 106 and 107 CFU g−1 wet weight on the biofilm of artificially introduced U. pertusa and 10 to 102 CFU mL−1 from adjacent seawater; however, GLB found from natural macroalgal species targeted all tested HAB species (five species), ranging between 105 and 106 CFU g−1 wet weight in density. These findings provide new ecological insights of GLB at macroalgal beds, and concurrently demonstrate the possible biological control of HABs by artificially introduced Ulva beds.

scholarly journals Dinoflagellate cysts in surface sediments at Van Phong Bay, Khanh Hoa Province, Vietnam: distribution, abundance and potentially harmful algal blooms

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Phan Tan Luom ◽  
Doan Nhu Hai ◽  
Nguyen Ngoc Lam
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Surface Sediments ◽  
Diversity Index ◽  
Dry Weight ◽  
The Other ◽  
Dinoflagellate Cysts ◽  
Toxic Dinoflagellate ◽  
Scrippsiella Trochoidea ◽  
Different Types

Studies on dinoflagellate cysts provide important knowledge on the ecology of dinoflagellates and harmful algal blooms (HABs). In this study, distribution and abundance of dinoflagellate cysts in surface sediments from 17 stations at Van Phong Bay were analyzed. There were 55 different types of cysts representing 3 orders and 18 genera, and 8 unidentified cyst types recorded. Peridiniales was the most diverse order with 29 cyst types, including 20 Protoperidinium cyst types. There were 10 cyst types of 7 potential toxic dinoflagellate species and 4 of bloom forming species found, indicating a potential risk of harmful algal blooms in Van Phong Bay. Number of cyst types and density ranged from 12 to 31 types and from 115 to 3,760 per gram of dry weight sediment, respectively. Cysts of Leonella granifera were dominant at stations in the mouth of the Bay, while Scrippsiella trochoidea cysts dominated at all stations. Shannon diversity index (H') was low, varying from 1.192.72. There were two distinct cyst assemblages identified with 40% Bray-Curtis similarity, assemblage I with 2 stations (VP09 and VP10), and assemblage II with the other stations. 

BIOASSAY OF PROLACTIN

1969 ◽  
Vol 60 (1) ◽  
pp. 91-100 ◽  
Author(s):  
Charles S. Nicoll
Keyword(s):  
Epithelial Cells ◽  
Lipid Content ◽  
Dry Weight ◽  
Fat Content ◽  
Assay Sensitivity ◽  
Mucosal Epithelium ◽  
Wet Weight ◽  
Total Dry Weight

ABSTRACT The response of the pigeon crop-sac to systemically acting prolactin (injected subcutaneously) was evaluated by measuring the wet weight of the responsive lateral lobes of the organ and by determining the dry weight of a 4 cm diameter disc of mucosal epithelium taken from one hemicrop. Of several different injection schedules tested, administration of prolactin in four daily injections was found to yield optimal responses. When compared with a graded series of prolactin doses, measurement of the mucosal dry weight proved to be a better method of response quantification than determination of the crop-sac wet weight with respect to both assay sensitivity and precision. The submucosal tissue of the crop-sac was estimated to constitute about 64 % of the total dry weight of the unstimulated organ and it was found to be relatively unresponsive to prolactin stimulation in comparison with the mucosa. The lipid content of the mucosal epithelium was determined using unstimulated crop-sacs or tissues which showed varying degrees of prolactin-induced proliferation. The fat content of the mucosal epithelial cells increased only slightly more rapidly than the dry weight or the defatted dry weight of the mucosa. Suggestions are made for the further improvement of the systemic crop-sac assay for prolactin.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

2018 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Yetti Elidar
Keyword(s):  
Organic Fertilizer ◽  
Dry Weight ◽  
Treatment Combination ◽  
Root Dry Weight ◽  
Randomized Design ◽  
Dose Study ◽  
Palm Sugar ◽  
Wet Weight ◽  
Completely Randomized Design ◽  
Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters. 

National coastal management challenges and needs

Shore & Beach ◽  
2020 ◽  
pp. 34-43
Author(s):  
Nicole Elko ◽  
Tiffany Roberts Briggs
Keyword(s):  
Coastal Management ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Local Community ◽  
Extreme Event ◽  
Technical Information ◽  
Interdisciplinary Approach ◽  
Beach Erosion ◽  
The U.S

In partnership with the U.S. Geological Survey Coastal and Marine Hazards and Resources Program (USGS CMHRP) and the U.S. Coastal Research Program (USCRP), the American Shore and Beach Preservation Association (ASBPA) has identified coastal stakeholders’ top coastal management challenges. Informed by two annual surveys, a multiple-choice online poll was conducted in 2019 to evaluate stakeholders’ most pressing problems and needs, including those they felt most ill-equipped to deal with in their day-to-day duties and which tools they most need to address these challenges. The survey also explored where users find technical information and what is missing. From these results, USGS CMHRP, USCRP, ASBPA, and other partners aim to identify research needs that will inform appropriate investments in useful science, tools, and resources to address today’s most pressing coastal challenges. The 15-question survey yielded 134 complete responses with an 80% completion rate from coastal stakeholders such as local community representatives and their industry consultants, state and federal agency representatives, and academics. Respondents from the East, Gulf, West, and Great Lakes coasts, as well as Alaska and Hawaii, were represented. Overall, the prioritized coastal management challenges identified by the survey were: Deteriorating ecosystems leading to reduced (environmental, recreational, economic, storm buffer) functionality, Increasing storminess due to climate change (i.e. more frequent and intense impacts), Coastal flooding, both Sea level rise and associated flooding (e.g. nuisance flooding, king tides), and Combined effects of rainfall and surge on urban flooding (i.e. episodic, short-term), Chronic beach erosion (i.e. high/increasing long-term erosion rates), and Coastal water quality, including harmful algal blooms (e.g. red tide, sargassum). A careful, systematic, and interdisciplinary approach should direct efforts to identify specific research needed to tackle these challenges. A notable shift in priorities from erosion to water-related challenges was recorded from respondents with organizations initially formed for beachfront management. In addition, affiliation-specific and regional responses varied, such as Floridians concern more with harmful algal blooms than any other human and ecosystem health related challenge. The most common need for additional coastal management tools and strategies related to adaptive coastal management to maintain community resilience and continuous storm barriers (dunes, structures), as the top long-term and extreme event needs, respectively. In response to questions about missing information that agencies can provide, respondents frequently mentioned up-to-date data on coastal systems and solutions to challenges as more important than additional tools.

PHYTOTOXICITY OF WASTEWATER CONTAINING CADMIUM (Cd) USING OLEANDER

2020 ◽  
Vol 51 (4) ◽  
pp. 1231-1238
Author(s):  
Zeki & Ridha
Keyword(s):  
Translocation Factor ◽  
Preliminary Test ◽  
Dry Weight ◽  
Exposure Dose ◽  
Test Duration ◽  
Plant Analysis ◽  
Wet Weight ◽  
Simulated Wastewater ◽  
Almost All ◽  
Plant Shoots

This study was aimed to investigate the ability of N.oleander to remove Cadmium (Cd) from wastewater. A prolonged toxicity test was performed in a single exposure and run for 65 days with various concentrations of Cd. Plants were grown in sand medium and irrigated with simulated wastewater contaminated with Cd, using different concentrations (0, 10, 25, 50, 75 mg/L), which were chosen based on previous preliminary test. The results of physical observation of the plants didn’t show any withering symptoms. The Cd concentration in plants increased, while in water decreased. The results of plant analysis showed that Cd concentration in plant shoots (stems and leaves) was higher than that in roots for almost all exposure doses along the test duration. The concentration of Cd in water decreased significantly from the first week of the test and become (0 mg/l) on day-35 for 10 and 25 mg/l exposure doses, while exceeded the permissible limits for 50 and 75 mg/l exposure doses and were 0.14 and 0.91 mg/l, respectively. Wet weight and dry weight of Oleander decreased with increasing Cd concentration level except for 10 mg/l exposure dose where the plant wet weight and dry weight increased at the end of the test. Bioaccumulation factor (BAF) and Translocation Factor (TF) was found to be greater than 1, indicating that Oleander is a successful hyperaccumulator for Cd.

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