Antioxidant and DNA Damage Protective Effects of Asparagus racemosus in Human Colon and Mice Muscle Cells

2015 ◽  
Vol 7 (3) ◽  
pp. 182-190 ◽  
Author(s):  
Girindrababu Venkattappa Jayashree ◽  
Puttasiddiah Rachitha ◽  
Krishnaswamy Krupashree ◽  
Kandikattu Hemanth Kumar ◽  
Farhath Khanum
Keyword(s):  
Dna Damage ◽  
Muscle Cells ◽  
Human Colon ◽  
Asparagus Racemosus ◽  
Protective Effects ◽  
Dna Damage Protective

scholarly journals Bioactive properties: enhancement of hepatoprotective, antioxidant and DNA damage protective effects of golden grey mullet protein hydrolysates against paracetamol toxicity

RSC Advances ◽  
2018 ◽  
Vol 8 (41) ◽  
pp. 23230-23240
Author(s):  
Intidhar Bkhairia ◽  
Sabah Dhibi ◽  
Rim Nasri ◽  
Abdelfettah Elfeki ◽  
Najla Hfaiyedh ◽  
...  
Keyword(s):  
Dna Damage ◽  
Liver Injury ◽  
Wistar Rats ◽  
Protein Hydrolysates ◽  
Protective Effects ◽  
Grey Mullet ◽  
Liza Aurata ◽  
Bioactive Properties ◽  
Paracetamol Toxicity ◽  
Dna Damage Protective

This study was undertaken to examine the hepatoprotective, antioxidant, and DNA damage protective effects of protein hydrolysates fromLiza aurata, against paracetamol overdose induced liver injury in Wistar rats.

scholarly journals Protective Effects of Delphinidin against Proton Beam Induced Damage in Human Colon-Derived CCD-18Co Cells

2017 ◽  
Author(s):  
Hyun Mi Kim ◽  
Seong Hee Kang ◽  
Suk Hee Kim ◽  
Bo Sun Kang
Keyword(s):  
Dna Damage ◽  
Antioxidant Enzymes ◽  
Proton Beam ◽  
Proton Beam Therapy ◽  
Human Colon ◽  
Protective Effects ◽  
Western Blot Assay ◽  
Colon Cells ◽  
Normal Tissues ◽  
Diphenyl Tetrazolium Bromide

Unavoidable exposure to radiosensitive normal tissues around cancerous tumor during the radiotherapy can cause side effects such as self-limited acute toxicities, mild chronic symptoms, or severe organ dysfunction. Nevertheless, clinical use of currently available radiation protective agents is limited because of their generic cytotoxicity. A study on radiation protective effect of delphinidin was conducted with proton-beam-exposed human colon cells (CCD-18Co). The measurement in changes of survival fractions of CCD-18Co with/without delphinidin administration at different radiation doses were measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The changes in expression of reactive oxygen species (ROS) and in activities of antioxidant enzymes were measured by colorimetric assays using pertinent assay kits. The measurement of pro-apoptosis/pro-survival protein expressions using Western blot assay and the measurement of DNA damage using comet assay were also fulfilled to evaluate the molecular level of radiation damages in CCD-18Co cells. The experimental results revealed that the pre-administration of delphinidin regulated antioxidant enzymes, reduced ROS, decreased DNA damage, regulated pro-apoptosis/pro-survival proteins, and eventually reduced apoptosis of CCD-18Co cells. In conclusion, it is claimed that delphinidin is nontoxic natural radiation protective compound, and thus delphinidin can be used to protect normal colon tissues during the proton beam therapy.

scholarly journals Olaparib-mediated enhancement of 5-fluorouracil cytotoxicity in mismatch repair deficient colorectal cancer cells

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Helena de Castro e Gloria ◽  
Laura Jesuíno Nogueira ◽  
Patrícia Bencke Grudzinski ◽  
Paola Victória da Costa Ghignatti ◽  
Temenouga Nikolova Guecheva ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Colon Cancer ◽  
Dna Damage ◽  
Cancer Cells ◽  
Mismatch Repair ◽  
Cell Cycle Progression ◽  
Combined Therapy ◽  
Combined Treatment ◽  
Human Colon ◽  
Human Colon Cancer

Abstract Background The advances in colorectal cancer (CRC) treatment include the identification of deficiencies in Mismatch Repair (MMR) pathway to predict the benefit of adjuvant 5-fluorouracil (5-FU) and oxaliplatin for stage II CRC and immunotherapy. Defective MMR contributes to chemoresistance in CRC. A growing body of evidence supports the role of Poly-(ADP-ribose) polymerase (PARP) inhibitors, such as Olaparib, in the treatment of different subsets of cancer beyond the tumors with homologous recombination deficiencies. In this work we evaluated the effect of Olaparib on 5-FU cytotoxicity in MMR-deficient and proficient CRC cells and the mechanisms involved. Methods Human colon cancer cell lines, proficient (HT29) and deficient (HCT116) in MMR, were treated with 5-FU and Olaparib. Cytotoxicity was assessed by MTT and clonogenic assays, apoptosis induction and cell cycle progression by flow cytometry, DNA damage by comet assay. Adhesion and transwell migration assays were also performed. Results Our results showed enhancement of the 5-FU citotoxicity by Olaparib in MMR-deficient HCT116 colon cancer cells. Moreover, the combined treatment with Olaparib and 5-FU induced G2/M arrest, apoptosis and polyploidy in these cells. In MMR proficient HT29 cells, the Olaparib alone reduced clonogenic survival, induced DNA damage accumulation and decreased the adhesion and migration capacities. Conclusion Our results suggest benefits of Olaparib inclusion in CRC treatment, as combination with 5-FU for MMR deficient CRC and as monotherapy for MMR proficient CRC. Thus, combined therapy with Olaparib could be a strategy to overcome 5-FU chemotherapeutic resistance in MMR-deficient CRC.

Ca2+ currents in human colonic smooth muscle cells

1995 ◽  
Vol 269 (3) ◽  
pp. G378-G385 ◽  
Author(s):  
Z. Xiong ◽  
N. Sperelakis ◽  
A. Noffsinger ◽  
C. Fenoglio-Preiser
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Voltage ◽  
Muscle Cells ◽  
Human Colon ◽  
Physiological Salt Solution ◽  
Channel Blockers ◽  
Threshold Potential ◽  
Tissue Samples ◽  
Inward Current

Voltage-gated Ca2+ currents were investigated in single smooth muscle cells freshly isolated from the circular layer of the human colon (ascending and descending portions) using the whole cell voltage-clamp technique. Tissue samples were obtained at the time of therapeutic surgery. In physiological salt solution (containing 2 mM Ca2+), an inward current was observed when the cell membrane was depolarized in the presence of tetrodotoxin. This current disappeared when Ca2+ was removed from the bath solution and was inhibited when Ca2+ channel blockers were applied, indicating that the inward current was a Ca2+ current (ICa). Changing the holding potential (HP) from -100 mV to more positive potentials (e.g., -60 and -40 mV) markedly decreased the amplitude of ICa. The voltage dependence of steady-state activation and inactivation was represented by Boltzmann distributions; there was a substantial amount of overlap (window current) between -60 and -10 mV. A fast-inactivating ICa component followed by a slow-inactivating ICa component was observed in some cells from both ascending and descending colons. The fast ICa component was observed only when cells were held at -80 or -100 mV, and had a more negative threshold potential (-70 to -60 mV). This component was sensitive to low concentrations of Ni2+ (30 microM) but was resistant to nifedipine (10-20 microM). In contrast, the slow (sustained) ICa component was observed at all HPs (-40 to -100 mV) and had a more positive threshold potential (about -40 mV). This component was insensitive to low concentration of Ni2+ but was sensitive to nifedipine and BAY K 8644.(ABSTRACT TRUNCATED AT 250 WORDS)

Assessment of the protective effects of selected dietary anticarcinogens against DNA damage and cytogenetic effects induced by benzo[a]pyrene in C57BL/6J mice

2011 ◽  
Vol 49 (8) ◽  
pp. 1674-1683 ◽  
Author(s):  
Dobrosława Gradecka-Meesters ◽  
Jadwiga Palus ◽  
Gabriela Prochazka ◽  
Dan Segerbäck ◽  
Elżbieta Dziubałtowska ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protective Effects ◽  
Cytogenetic Effects

O XVII B.2 Determination of endogenous DNA damage, oxidated DNA bases and oxidative stress in primary human colon cells and modulation by plant ingredients

1997 ◽  
Vol 379 (1) ◽  
pp. S171
Author(s):  
Beatrice L. Pool-Zobel ◽  
Salomon L. Abrahamse ◽  
Daniela Oberreuther ◽  
Sylvia Treptow-van Lishaut ◽  
Gerhard Rechkemmer
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Human Colon ◽  
Dna Bases ◽  
Colon Cells ◽  
And Oxidative Stress

scholarly journals Low levels of methylmercury induce DNA damage in rats: protective effects of selenium

2008 ◽  
Vol 83 (3) ◽  
pp. 287-287
Author(s):  
Denise Grotto ◽  
Gustavo R. M. Barcelos ◽  
Juliana Valentini ◽  
Lusânia M. G. Antunes ◽  
José Pedro F. Angeli ◽  
...  
Keyword(s):  
Dna Damage ◽  
Protective Effects ◽  
Low Levels

8. Evaluation of Snow Fungus Polysaccharides on benzo[a]pyrene-induced DNA Damage

2018 ◽  
Author(s):  
Daisy Liu
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Free Radical ◽  
Radical Scavenging ◽  
Chinese Hamster ◽  
Negative Control ◽  
Lung Fibroblasts ◽  
Protective Effects ◽  
Tremella Fuciformis

Snow fungus, Tremella fuciformis, has been demonstrated to have numerous health benefits including purported chemopreventive properties due to free radical-scavenging ability. Protective effects derived from snow fungus polysaccharides are evaluated on Chinese hamster lung fibroblasts (CCL-39) exposed to carcinogen benzo[a]pyrene known to cause free radical formation and oxidative stress to cells. In this experiment, it was hypothesized that the naturally occurring polysaccharides in snow fungus are able to protect against or reduce oxidative stress-induced DNA damage. Polysaccharides were isolated through an alkaline extraction and in-vitro digestion. DNA damage was measured using the single-cell gel electrophoresis comet assay after exposure to benzo[a]pyrene and polysaccharide extract to lung fibroblasts. Results were calculated using the mean and standard deviation data of tail length and area, respectively. Each damaged cell was measured and analyzed through ImageJ Editing Software. The results indicate a promising trend which depict snow fungus polysaccharides yielding lower levels of DNA damage compared to cells exposed to benzo[a]pyrene and compared to the negative control (phosphate buffered saline and Dulbecco’s cell medium). This study suggests polysaccharides from Tremella fuciformis could truly prevent cellular DNA damage by protecting against oxidative stress.

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