Development and Validation of a Stability-Indicating Reverse Phase HPLC-PDA Method for Determination of Canagliflozin in Bulk and Pharmaceutical Dosage Form

2016 ◽  
Vol 7 (1) ◽  
pp. 54-62 ◽  
Author(s):  
Ishpreet Kaur ◽  
Sharad Wakode ◽  
Harsharan Pal Singh ◽  
Satish Manachanda
Keyword(s):  
Dosage Form ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Development And Validation

scholarly journals The Development and Validation of a Stability-Indicating UHPLC-DAD Method for Determination of Perindopril l-Arginine in Bulk Substance and Pharmaceutical Dosage Form

2014 ◽  
Vol 77 (21-22) ◽  
pp. 1497-1501 ◽  
Author(s):  
Magdalena Paczkowska ◽  
Przemysław Zalewski ◽  
Piotr Garbacki ◽  
Alicja Talaczyńska ◽  
Anna Krause ◽  
...  
Keyword(s):  
Dosage Form ◽  
Pharmaceutical Dosage Form ◽  
Stability Indicating ◽  
Development And Validation

scholarly journals Development and Validation of Reverse Phase HPLC Method for Simultaneous Estimation of Allopurinol and Lesinurad in its API and Pharmaceutical Dosage Form

2019 ◽  
Vol 4 (04) ◽  
pp. 50-57
Author(s):  
Sayma Khader ◽  
Ayesha Begum K ◽  
D. Ramakrishna
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage

A new, reliable, and validated reverse phase-high-performance liquid chromatography (HPLC) method was developed to quantify the amount of allopurinol and lesinurad simultaneously in solid (tablet) dosage form. A clear chromatographic division was attained on inertsil ODS (4.6 x 250 mm, 5 mm) column, and a mixture of 0.1% trifluoroacetic acid and methanol in the ratio of 40:60 v/v was used as mobile phase. The rate of flow was set at 1 mL/min, and UV detection was achieved at λmax of 255 nm. Injection volume was set to 20 μL. The correlation coefficient of 0.999 was established, and the accurateness was found to be 100.69 and 100.49 for both the drugs, respectively. Therefore, the developed method was simple, specific, precise, and stable. Hence, the method can be employed to estimate the said drugs in other pharmaceutical formulations.

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