scholarly journals Formulation of Alkaloid Loaded Phytosomes from Tinospora cordifolia and ex-vivo Intestinal Permeability Study

2021 ◽  
Vol 55 (2) ◽  
pp. 474-482
Author(s):  
Aishwarya Laxman Thakur ◽  
Kalpana Sabanna Patil
Keyword(s):  
Intestinal Permeability ◽  
Ex Vivo ◽  
Tinospora Cordifolia ◽  
Permeability Study

scholarly journals Formulation and evaluation of solid self micro emulsifying drug delivery system using aerosil 200 as solid carrier

2012 ◽  
Vol 1 (12) ◽  
pp. 414-419 ◽  
Author(s):  
Durgacharan Arun Bhagwat ◽  
John Intru D’Souza
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Intestinal Permeability ◽  
Delivery System ◽  
Ex Vivo ◽  
Water Soluble ◽  
Solid Carrier ◽  
Drug Content ◽  
Permeability Study ◽  
Poorly Water Soluble

Improvement of bio-availability of poorly water soluble drugs presents one of the furthermost challenge in drug formulations. One of the most admired and commercially viable formulation approach for this challenge is solid self micro emulsifying drug delivery system (S-SMEDDS). There are many techniques to convert liquid SMEDDS to solid, but an adsorption technique is simple and economic. Hence aim of present study was to develop S-SMEDDS of poorly water soluble drug Telmisartan (TEL) using Aerosil 200 as solid carrier. Liquid SMEDDS was prepared using Acrysol EL 135, Tween 80 and PEG 400 as oil, surfactant and co-surfactant and was converted to S-SMEDDS by adsorbing it on Aerosil 200. Prepared S-SMEDDS was evaluated for flow properties, drug content, reconstitution properties, DSC, SEM, in-vitro drug release and ex-vivo intestinal permeability study. Results showed that prepared S-SMEDDS have good flow property with 99.45 ± 0.02% drug content. Dilution study by visual observation showed that there was spontaneous micro emulsification and no sign of phase separation. Droplet size was found to be 0.34 µm with polydispersity index of 0.25. DSC thermogram showed that crystallization of TEL was inhibited. SEM photograph showed smooth surface of S-SMEDDS with less aggregation. Drug releases from S- SMEDDS were found to be significantly higher as compared with that of plain TEL. Ex-vivo intestinal permeability study revealed that diffusion of drug was significantly higher from S-SMEDDS than that of suspension of plain TEL. Study concluded that S-SMEDDS can effectively formulated by adsorption technique with enhanced dissolution rate and concomitantly bioavailability.DOI: http://dx.doi.org/10.3329/icpj.v1i12.12451 International Current Pharmaceutical Journal 2012, 1(12): 414-419

The potential of porcine ex vivo platform for intestinal permeability screening of FcRn-targeted drugs

2021 ◽  
Vol 162 ◽  
pp. 99-104
Author(s):  
Cláudia Azevedo ◽  
Jan Terje Andersen ◽  
Giovanni Traverso ◽  
Bruno Sarmento
Keyword(s):  
Intestinal Permeability ◽  
Ex Vivo ◽  
Targeted Drugs

scholarly journals Intestinal Permeability Assays: a Review

2021 ◽  
Vol 31 (1) ◽  
pp. 20-30
Author(s):  
A. A. Iakupova ◽  
S. R. Abdulkhakov ◽  
R. K. Zalyalov ◽  
A. G. Safin ◽  
R. A. Abdulkhakov
Keyword(s):  
Intestinal Permeability ◽  
Ex Vivo ◽  
Venous Blood ◽  
Intestinal Barrier ◽  
Intestinal Lumen ◽  
Alcoholic Fatty Liver ◽  
Key Points ◽  
Assessment Techniques ◽  
Portal Venous Blood

Aim. A literature review of intestinal permeability assessment techniques.Key points. The intestinal barrier is a functional entity separating the intestinal lumen and internal body, and intestinal permeability is a measure of the barrier functionality. The intestinal barrier integrity and permeability assays differ by the application setting (in vivo or ex vivo), subject (human or animal), marker molecules used to assess permeability (ions, various size carbohydrates, macromolecules, antigens, bacterial products and bacteria), biomaterial for the marker concentration assays (peripheral blood, portal venous blood, urine, stool). Despite a great variety of methods for assessing intestinal permeability, their clinical application requires further studies due to a lack of standardisation, the complexity of selected techniques and occasional limited reliability of results.Conclusion. Further investigation and improvement of intestinal permeability assays is required. The assay and result standardisation will facilitate practice in functional and organic intestinal diseases, as well as allergies, diabetes mellitus, non-alcoholic fatty liver disease and some other illnesses.

scholarly journals Preparation, Characterization and Ex vivo Permeability Study of Transdermal Apixaban O/W Nanoemulsion Based Gel

2020 ◽  
Vol 29 (2) ◽  
pp. 214-222
Author(s):  
Mustafa R. Abdulbaqi ◽  
Nawal A.Rajab
Keyword(s):  
Water Solubility ◽  
Ex Vivo ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Good Method ◽  
Gelling Agent ◽  
Permeation Enhancer ◽  
Permeability Study ◽  
Anticoagulant Drug ◽  
Triton X

This study designed to prepare ultrafine apixaban (APX) o/w nanoemulsion (NE) based gel with droplet size below 50 nm as a good method for transdermal APX delivery without using permeation enhancer, alternatively, the formulation components itself act as permeation enhancer. APX, a potent oral anticoagulant drug that selectively and directly inhibit coagulation factor Xa, was selected as a good candidate for transdermal delivery as it displays poor water solubility (0.028 mg/mL) and low bioavailability (50%). APX-NE gel was prepared using triacetin, triton-x-100 and carbitol as oil phase, surfactant and cosurfactant respectively, while Carbopol 940 used as a gelling agent. Ex vivo permeation of APX-NE gel through human stratum corneum reveal

Lipid-nanopotentiated combinatorial delivery of tamoxifen and sulforaphane: ex vivo, in vivo and toxicity studies

Nanomedicine ◽  
2020 ◽  
Vol 15 (26) ◽  
pp. 2563-2583 ◽  
Author(s):  
Bharti Mangla ◽  
Yub R Neupane ◽  
Archu Singh ◽  
Pankaj Kumar ◽  
Sadat Shafi ◽  
...  
Keyword(s):  
Intestinal Permeability ◽  
Oral Delivery ◽  
Chemotherapeutic Agent ◽  
Treatment Approach ◽  
Ex Vivo ◽  
Toxicity Assessment ◽  
Drug Release Profile ◽  
Novel Treatment

Aim: This study aims to load tamoxifen (TAM) and sulforaphane (SFN) into nanostructured lipid carriers (NLCs) to enhance their oral delivery. Materials & methods: TAM-SFN-NLCs were prepared using Precirol® ATO5 and Transcutol® HP, characterized and evaluated in vitro and ex vivo to assess the drug release profile and intestinal permeability, respectively. In vivo pharmacokinetic and acute toxicity assessment was performed in Wistar rats. Results: Optimized TAM-SFN-NLCs exhibited a particle size of 121.9 ± 6.42 nm and zeta potential of -21.2 ± 2.91 mV. The NLCs enhanced intestinal permeability of TAM and SFN and augmented oral bioavailability of TAM and SFN 5.2-fold and 4.8-fold, respectively. SFN significantly reduced TAM-associated toxicity in vivo. Conclusion: This coencapsulation of a chemotherapeutic agent with a herbal bioactive in NLCs could pave a novel treatment approach against cancer.

Gliadin induces increased intestinal permeability, zonulin release, and occludin down-regulation in an Ex-vivo human intestinal model of celiac disease

2003 ◽  
Vol 124 (4) ◽  
pp. A658 ◽  
Author(s):  
Sandro Drago ◽  
Ramzi El Asmar ◽  
Cinzia D'Agate ◽  
Giuseppe Iacono ◽  
Mariarosaria Di Pierro ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Intestinal Permeability ◽  
Ex Vivo ◽  
Down Regulation

scholarly journals Long-chain inulin increases dendritic cells in the Peyer's patches and increasesex vivocytokine secretion in the spleen and mesenteric lymph nodes of growing female rats, independent of zinc status

2008 ◽  
Vol 101 (11) ◽  
pp. 1653-1663 ◽  
Author(s):  
Natasha R. Ryz ◽  
Jon B. Meddings ◽  
Carla G. Taylor
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
Lymph Nodes ◽  
Immune Function ◽  
Intestinal Permeability ◽  
Ex Vivo ◽  
Female Rats ◽  
Mesenteric Lymph Nodes ◽  
Cell Numbers ◽  
Mesenteric Lymph

Prebiotics may increase Zn absorption, a mineral known to play a central role in the immune system. Zn-deficient states are characterised by suppressed immune function, while prebiotics may improve both gut and cell-mediated immunity. Our objective was to determine if inulin alters the number and proportion of immune cells in the spleen, mesenteric lymph nodes (MLN) and Peyer's patches (PP),ex vivocytokine secretion, intestinal permeability and Zn status in healthy as well as Zn-deficient rats. Weanling female rats were fed diets supplemented with 5 % cellulose (CEL) or 5 % inulin (PRE) for 4 weeks. The rats received the CEL or PRE dietad libitum(ZN) or in restricted amounts (DR), or deficient in Zn (ZD) for another 4 weeks. The PRE-fed rats had a higher number and proportion of dendritic cells in PP, and greaterex vivosecretion of IL-2, IL-10 and interferon-γ from spleen and MLN cells compared with CEL-fed rats. PRE reduced the number and proportion of T cell receptor (TCR)-αβ+CD8+cells in spleen and CD45RA+cells in MLN compared with CEL. ZD rats had lower serum IgG2a and T cell numbers in MLN compared with ZN and DR rats. TCRγδ+cell numbers in PP were higher in ZD-PRE rats compared with ZD-CEL rats. Femur Zn concentrations of DR-PRE rats were higher than those of DR-CEL rats. Intestinal permeability was unchanged. The higher proportion and number of dendritic cells in the PP of inulin-fed rats indicates a need for further research on how prebiotics and their metabolites affect immune function possibly through intestinal dendritic cells.

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