scholarly journals Electrospun Silybin Enriched Scaffolds of Polyethylene Oxide as Wound Dressings: Enhanced Wound Closure, Reepithelization in Rat Excisional Wound Model

2019 ◽  
Vol 53 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Prajakta Deepak Kapadnis ◽  
Shilpa Nilesh Shrotriya
Keyword(s):  
Polyethylene Oxide ◽  
Wound Closure ◽  
Wound Dressings ◽  
Wound Model ◽  
Excisional Wound

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

scholarly journals The murine excisional wound model: Contraction revisited

2015 ◽  
Vol 23 (6) ◽  
pp. 874-877 ◽  
Author(s):  
Lin Chen ◽  
Rita Mirza ◽  
Young Kwon ◽  
Luisa A. DiPietro ◽  
Timothy J. Koh
Keyword(s):  
Wound Model ◽  
Excisional Wound

The topical administration of rhEGF-loaded nanostructured lipid carriers (rhEGF-NLC) improves healing in a porcine full-thickness excisional wound model

2015 ◽  
Vol 197 ◽  
pp. 41-47 ◽  
Author(s):  
Garazi Gainza ◽  
Diego Celdran Bonafonte ◽  
Beatriz Moreno ◽  
José Javier Aguirre ◽  
Francisco Borja Gutierrez ◽  
...  
Keyword(s):  
Topical Administration ◽  
Nanostructured Lipid Carriers ◽  
Full Thickness ◽  
Wound Model ◽  
Excisional Wound

scholarly journals Induced pluripotent stem cell-derived endothelial cells promote angiogenesis and accelerate wound closure in a murine excisional wound healing model

2018 ◽  
Vol 38 (4) ◽  
Author(s):  
Zoë E. Clayton ◽  
Richard P. Tan ◽  
Maria M. Miravet ◽  
Katarina Lennartsson ◽  
John P. Cooke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Adult Stem Cells ◽  
Wound Closure ◽  
Chronic Wounds ◽  
Control Treatment ◽  
Excisional Wound ◽  
Induced Pluripotent

Chronic wounds are a major complication in patients with cardiovascular diseases. Cell therapies have shown potential to stimulate wound healing, but clinical trials using adult stem cells have been tempered by limited numbers of cells and invasive procurement procedures. Induced pluripotent stem cells (iPSCs) have several advantages of other cell types, for example they can be generated in abundance from patients’ somatic cells (autologous) or those from a matched donor. iPSCs can be efficiently differentiated to functional endothelial cells (iPSC-ECs). Here, we used a murine excisional wound model to test the pro-angiogenic properties of iPSC-ECs in wound healing. Two full-thickness wounds were made on the dorsum of NOD-SCID mice and splinted. iPSC-ECs (5 × 105) were topically applied to one wound, with the other serving as a control. Treatment with iPSC-ECs significantly increased wound perfusion and accelerated wound closure. Expression of endothelial cell (EC) surface marker, platelet endothelial cell adhesion molecule (PECAM-1) (CD31), and pro-angiogenic EC receptor, Tie1, mRNA was up-regulated in iPSC-EC treated wounds at 7 days post-wounding. Histological analysis of wound sections showed increased capillary density in iPSC-EC wounds at days 7 and 14 post-wounding, and increased collagen content at day 14. Anti-GFP fluorescence confirmed presence of iPSC-ECs in the wounds. Bioluminescent imaging (BLI) showed progressive decline of iPSC-ECs over time, suggesting that iPSC-ECs are acting primarily through short-term paracrine effects. These results highlight the pro-regenerative effects of iPSC-ECs and demonstrate that they are a promising potential therapy for intractable wounds.

A low-swelling and toughened adhesive hydrogel with anti-microbial and hemostatic capacities for wound healing

2022 ◽  
Author(s):  
Liwei Zhang ◽  
Yajie Zhang ◽  
Fanshu Ma ◽  
Xingzhu Liu ◽  
Yangzhong Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Wound Dressings ◽  
Tissue Adhesion

Hydrogel-based wound dressings with tissue adhesion are widely used for wound closure. However, currently developed hydrogel adhesives are still difficult to continue sealing the wound under the bleeding condition. Herein,...

scholarly journals Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

2020 ◽  
Vol 11 ◽  
pp. 204173142094933 ◽  
Author(s):  
Hye Sung Kim ◽  
Junyu Chen ◽  
Lin-Ping Wu ◽  
Jihua Wu ◽  
Hua Xiang ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Scar Formation ◽  
Dermal Fibroblasts ◽  
Wound Dressings ◽  
Elongation At Break ◽  
Wound Model ◽  
Solution Cast ◽  
Model Treatment

To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate- co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cast films or electrospun nanofibrous meshes. As 3HV content increases, the crystallinity decreases and the scaffolds become more elastic. The nanofibrous meshes exhibit greater elasticity and elongation at break than films. When used to culture human dermal fibroblasts in vitro, PHBV meshes give better cell attachment and proliferation, less differentiation to myofibroblasts, and less substrate contraction. In a full-thickness mouse wound model, treatment with films or meshes enables regeneration of pale thin tissues without scabs, dehydration, or tubercular scar formation. The epidermis of wounds treated with meshes develop small invaginations in the dermis within 2 weeks, indicating hair follicle and sweat gland regeneration. Consistent with the in vitro results, meshes reduce myofibroblast differentiation in vivo through downregulation of α-SMA and TGF-β1, and upregulation of TGF-β3. The regenerated wounds treated with meshes are softer and more elastic than those treated with films. These results demonstrate that electrospun nanofibrous PHBV meshes mitigate excessive scar formation by regulating myofibroblast formation, showing their promise for use as wound dressings.

scholarly journals Autologous Graft Thickness Affects Scar Contraction and Quality in a Porcine Excisional Wound Model

2015 ◽  
Vol 3 (7) ◽  
pp. e468 ◽  
Author(s):  
Rodney K. Chan ◽  
Lloyd F. Rose ◽  
Jesse C. Wu ◽  
David I. Tucker ◽  
Maren M. Chan ◽  
...  
Keyword(s):  
Autologous Graft ◽  
Wound Model ◽  
Graft Thickness ◽  
Excisional Wound ◽  
Scar Contraction

Laser phototherapy on rat excisional wound model: Biomechanical analysis

2016 ◽  
Vol 32 ◽  
pp. e87-e88
Author(s):  
J.R. De Castro ◽  
F.S. Pereira ◽  
R.Y. Ballester ◽  
V.E. Arana-Chavez ◽  
A. Simoes
Keyword(s):  
Biomechanical Analysis ◽  
Wound Model ◽  
Laser Phototherapy ◽  
Excisional Wound

Efficacy of MMP-inhibiting wound dressings in the treatment of chronic wounds: a systematic review

2020 ◽  
Vol 29 (2) ◽  
pp. 102-118 ◽  
Author(s):  
Joachim Dissemond ◽  
Matthias Augustin ◽  
Michael Dietlein ◽  
Uta Faust ◽  
Winfried Keuthage ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Closure ◽  
Clinical Evidence ◽  
Chronic Wounds ◽  
Evidence Base ◽  
Healing Time ◽  
Size Reduction ◽  
Wound Dressings ◽  
Wound Size ◽  
Different Types

Objective: Matrix metalloproteinases (MMPs) substantially contribute to the development of chronicity in wounds. Thus, MMP-inhibiting dressings may support healing. A systematic review was performed to determine the existing evidence base for the treatment of hard-to-heal wounds with these dressings. Methods: A systematic literature search in databases and clinical trial registers was conducted to identify randomised controlled trials (RCTs) investigating the efficacy of MMP-inhibiting dressings. Studies were analysed regarding their quality and clinical evidence. Results: Of 721 hits, 16 relevant studies were assessed. There were 13 studies performed with collagen and three with technology lipido-colloid nano oligosaccharide factor (TLC-NOSF) dressings. Indications included diabetic foot ulcers, venous leg ulcers, pressure ulcers or wounds of mixed origin. Patient-relevant endpoints comprised wound size reduction, complete wound closure, healing time and rate. Considerable differences in the quality and subsequent clinical evidence exist between the studies identified. Substantial evidence for significant improvement in healing was identified only for some dressings. Conclusion: Evidence for the superiority of some MMP-inhibiting wound dressings exists regarding wound closure, wound size reduction, healing time and healing rate. More research is required to substantiate the existing evidence for different types of hard-to-heal wounds and to generate evidence for some of the different types of MMP-inhibiting wound dressings.

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