scholarly journals Fluorescence in situ hybridization on enriched CD138-positive cells in plasma cell myeloma

2015 ◽  
Vol 2 (2) ◽  
pp. 168
Author(s):  
Renu Bajaj ◽  
Yu Shi ◽  
Charalambos Solomides ◽  
Gerald Gong ◽  
Zi-Xuan(Zoe) Wang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Plasma Cell Myeloma

scholarly journals Molekuláris citogenetikai vizsgálatok Baranya és Tolna megye plazmasejtes myelomában szenvedő betegein

2019 ◽  
Vol 160 (24) ◽  
pp. 944-951
Author(s):  
Szabolcs Kosztolányi ◽  
Bálint Horváth ◽  
Diána Hosnyánszki ◽  
László Kereskai ◽  
Erzsébet Sziládi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Chain Gene ◽  
Plasma Cell Myeloma ◽  
Genetic Aberrations ◽  
Molecular Cytogenetic ◽  
Combined Application ◽  
Dependent Probe

Abstract: Introduction: Plasma cell myeloma is a hematological malignancy with heterogeneous genomic landscape and diverse clinical course. Recurrent chromosomal and subchromosomal aberrations commonly occur in this entity and are associated with the pathogenesis and progression of the disease. The identification of these alterations aids genetic characterization, classification and prognostication of patients. Aim: Molecular cytogenetic investigations of plasma cell myeloma patients treated at the University of Pécs Clinical Center and János Balassa County Hospital of Tolna County, Szekszárd, between 2005 and 2018 were evaluated in our study. Method: 231 patients were screened for genetic aberrations using fluorescence in situ hybridization. Translocations involving the immunoglobulin heavy chain gene, losses of 1p and 17p chromosome arms, gains of 1q chromosome arm and unbalanced aberrations of chromosome 13 were investigated. Losses and gains of 1p, 1q, 5q, 12p, 13q, 16q and 17p chromosome arms were analyzed using multiplex ligation-dependent probe amplification in 42 patients. During the investigated period, 116 bone marrow karyotyping was also performed. Results: In total, 233 genetic aberrations were identified using our targeted approaches; the frequency of specific aberrations correlated with data of the recent literature. Concordance of results gained by fluorescence in situ hybridization and multiplex ligation-dependent probe amplification was 96.2% by analyzing the same chromosome arms. The latter technique revealed 21 additional genetic aberrations in 16/42 patient samples (38%) as compared to fluorescence in situ hybridization. Conclusions: Our results suggest that the combined application of the two molecular cytogenetic methods may facilitate a more detailed characterization of genetic aberrations of plasma cell myeloma patients in Hungary. Orv Hetil. 2019; 160(24): 944–951.

Cytogenetic and fluorescence in situ hybridization studies in 60 patients with multiple myeloma and plasma cell leukemia

2004 ◽  
Vol 148 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Elisabet Lloveras ◽  
Isabel Granada ◽  
Lurdes Zamora ◽  
Blanca Espinet ◽  
Lourdes Florensa ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia

Fluorescence In Situ Hybridization Studies in 22 Cases of Plasma Cell Leukemia.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4461-4461
Author(s):  
Jianyong Li ◽  
Wei Xu ◽  
Lijuan Chen ◽  
Yu Zhu ◽  
Jinlan Pan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Cell Leukemia ◽  
Molecular Cytogenetic ◽  
Cytogenetic Aberrations ◽  
Plasma Cell Disorder ◽  
Igh Rearrangement ◽  
Cell Disorder

Abstract Background Plasma cell leukemia (PCL) is a rare malignant plasma cell disorder that usually carries an aggressive course with a rapidly fatal outcome. Cytogenetic studies performed on plasma cell disorder are scarce and difficult because of the low proliferation rate of plasma cells. Fluorescence in situ hybridization (FISH) analysis is an attractive alternative for evaluation of numerical and structural chromosomal changes in PCL. Methods Interphase FISH (I-FISH) and two different specific probes for the regions containing 13q14.3 (D13S319) or 14q32 (IGHC/IGHV) were used to detect 13q14 deletion [del(13q14)] or IgH rearrangement in 22 PCL patients. For patients with IgH rearrangement, probes for IgH(JH) and 11q13 (CCND1) or 4p16 (FGFR3) were used to detect t(11;14)(q13;q32) or t(4;14)(p16;q32). Results Molecular cytogenetic aberrations were found in 19 of 22 (86.4%) PCL patients. Del(13q14) was detected in 13 cases (59.1%), and IgH rearrangement in 17 (77.3%) patients including 7 with t(11;14) and 3 with t(4;14). 14q32 rearrangement and 13q14 deletion were found concurrently in 11 cases (50%). Conclusions Chromosomal abnormalities are frequent in PCL. The most frequent aberrations among the cases was the 14q32 rearrangement and 13q14 deletion. I-FISH technique is useful to detect molecular cytogenetic aberrations and should be used in the routine evaluation of PCL.

scholarly journals Target fluorescence in-situ hybridization (Target FISH) for plasma cell enrichment in myeloma

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Edmond S. K. Ma ◽  
Candy L. N. Wang ◽  
Anthony T. C. Wong ◽  
Gigi Choy ◽  
Tsun Leung Chan
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Cell Enrichment ◽  
Target Fish
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