scholarly journals Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, Sao Paulo, Brazil

Fottea ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Guilherme S. Hentschke ◽  
Janaina Rigonato ◽  
Diego B. Genuario ◽  
Haywood D. Laughinghouse IV ◽  
Celia L. Sant'Anna
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
Atlantic Rainforest ◽  
São Paulo ◽  
Morphological And Molecular Characterization

scholarly journals Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state

2019 ◽  
Vol 10 (4) ◽  
pp. 505-508
Author(s):  
Evelynne Urzedo Leão ◽  
Kelly Cristina Gonçales Rocha ◽  
Norberto Silva ◽  
Marcelo Agenor Pavan ◽  
Gentil Cavalheiro Adorian ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Molecular Characterization ◽  
Citrullus Lanatus ◽  
Sao Paulo ◽  
Morphological Observation ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State ◽  
Morphological And Molecular Characterization

Powdery mildew is one of the most commom foliar diseases of cultivated and wild cucurbits worldwide. Six different fungal species have been reported to be associated with powdery mildew of cucurbits, such as Podosphaera xanthii and Golovinomyces cichoracearum. The aim of the study was to identify the causal agent of the disease in watermelon plants (Citrullus lanatus) cultivated in greenhouses of the State of São Paulo. DNA total was extracted using Chelex method and the ITS region was amplified via standard PCR reaction using the specific primers PMITS1 and PMITS2. A fragment of 700pb was amplified and the sequence showed 99% of identity with P. xanthii (accession number JQ728480). Morphological observation showed that the conidia were ellipsoidal-ovoid, had fibrosin bodies and a bifurcated germinative tube in the lateral position. These characteristics and the molecular analyses confirmed P. xanthi as the etiological agent of powdery mildew in watermelon plants in São Paulo State. Morphological and molecular characterization of powdery mildew on watermelon plants in São Paulo state

scholarly journals Identification and Molecular Characterization of Norovirus in São Paulo State, Brazil

2008 ◽  
Vol 39 (4) ◽  
pp. 619-622 ◽  
Author(s):  
Simone Guadagnucci Morillo ◽  
Cilli Audrey ◽  
Rita de Cássia Compagnoli Carmona ◽  
Maria do Carmo Sampaio Tavares Timenetsky
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

scholarly journals Molecular Characterization of Mediterranean Spotted Fever Rickettsia Isolated From a European Traveler in the State of São Paulo, Brazil

2013 ◽  
Vol 20 (1) ◽  
pp. 54-56 ◽  
Author(s):  
Flávia S. Gehrke ◽  
Rodrigo N. Angerami ◽  
Mauro T. Marrelli ◽  
Eliana R. de Souza ◽  
Elvira M.M. do Nascimento ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Spotted Fever ◽  
Sao Paulo ◽  
The State ◽  
Mediterranean Spotted Fever ◽  
São Paulo ◽  
Spotted Fever Rickettsia

scholarly journals Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis

2008 ◽  
Vol 50 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Terezinha Lisieux Moraes Coimbra ◽  
Raimundo N. Santos ◽  
Selma Petrella ◽  
Teresa Keico Nagasse-Sugahara ◽  
Silvana Beres Castrignano ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Clinical Manifestations ◽  
Cross Reactivity ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Paulo State ◽  
São Paulo State

Rocio virus (ROCV) was responsible for an explosive encephalitis epidemic in the 1970s affecting about 1,000 residents of 20 coastland counties in São Paulo State, Brazil. ROCV was first isolated in 1975 from the cerebellum of a fatal human case of encephalitis. Clinical manifestations of the illness are similar to those described for St. Louis encephalitis. ROCV shows intense antigenic cross-reactivity with Japanese encephalitis complex (JEC) viruses, particularly with Ilheus (ILHV), St. Louis encephalitis, Murray Valley and West Nile viruses. In this study, we report a specific RT-PCR assay for ROCV diagnosis and the molecular characterization of the SPAn37630 and SPH37623 strains. Partial nucleotide sequences of NS5 and E genes determined from both strains were used in phylogenetic analysis. The results indicated that these strains are closely related to JEC viruses, but forming a distinct subclade together with ILHV, in accordance with results recently reported by Medeiros et al. (2007).

Detection and molecular characterization of Cryptosporidium species and Giardia assemblages in two watersheds in the metropolitan region of São Paulo, Brazil

2018 ◽  
Vol 25 (15) ◽  
pp. 15191-15203 ◽  
Author(s):  
Ronalda Silva de Araújo ◽  
Bruna Aguiar ◽  
Milena Dropa ◽  
Maria Tereza Pepe Razzolini ◽  
Maria Inês Zanoli Sato ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
Cryptosporidium Species ◽  
Metropolitan Region ◽  
São Paulo

scholarly journals Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil

2015 ◽  
Vol 8 (1) ◽  
pp. 103 ◽  
Author(s):  
Érica David ◽  
Semíramis Guimarães ◽  
Ana de Oliveira ◽  
Teresa Goulart de Oliveira-Sequeira ◽  
Gabriela Nogueira Bittencourt ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Sao Paulo ◽  
The State ◽  
São Paulo ◽  
Intestinal Protozoa ◽  
Poor Communities

scholarly journals Molecular characterization of vancomycin-resistant Enterococci strains eight years apart from its first isolation in São Paulo, Brazil

2008 ◽  
Vol 50 (4) ◽  
pp. 195-198 ◽  
Author(s):  
Pedro Alves d'Azevedo ◽  
Guilherme H.C. Furtado ◽  
Eduardo A.S. Medeiros ◽  
Kelly Aline Santiago ◽  
Suzane Silbert ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Genetic Relatedness ◽  
Sao Paulo ◽  
São Paulo ◽  
Pfge Analysis ◽  
Predominant Species ◽  
Vana Gene ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

E. faecium was the first reported VRE species, carrying the vanA gene in Brazil. In spite of this, vancomycin-resistant E. faecalis has become the predominant species in Brazilian hospitals. The aim of this study was to evaluate the genetic relatedness of VREs isolated in a Brazilian teaching hospital eight years apart from its first isolation. We analyzed 38 VRE strains obtained from 81 surveillance cultures of patients admitted to the four largest intensive care units in Hospital São Paulo in February, 2006. Presence of the vanA gene was assayed by PCR and PFGE analysis was used for molecular characterization. All VRE strains carried the vanA gene. Two distinct clonal groups were observed among vancomycin-resistant E. faecalis. Vancomycin-resistant E. faecium belonged to five distinct clones were demonstrated by molecular typing. All of these clones were different from the first vancomycin-resistant enterococci clone isolated eight years ago in our hospital.

Sign in / Sign up

Export Citation Format

Share Document