Porous Coated Titanium Implants do Not Inhibit Mesenchimal Stem Cells Proliferation and Osteogenic Differentiation

2013 ◽  
Vol 27 (6) ◽  
pp. 4290-4293 ◽  
Author(s):  
Boris Antonov ◽  
Ivan Bochev ◽  
Milena Mourdjeva ◽  
Plamen Kinov ◽  
Lubomir Tzvetanov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Titanium Implants

scholarly journals Osteogenic Differentiation of Human Mesenchymal Stem Cells Modulated by Surface Manganese Chemistry in SLA Titanium Implants

2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Jin-Woo Park ◽  
Yusuke Tsutsumi ◽  
Eui-Kyun Park
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Corrosion Resistance ◽  
Osteogenic Differentiation ◽  
Chemical Treatment ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Potential Candidate ◽  
Implant Surface ◽  
Wet Chemical

The manganese (Mn) ion has recently been probed as a potential candidate element for the surface chemistry modification of titanium (Ti) implants in order to develop a more osteogenic surface with the expectation of taking advantage of its strong binding affinity to the integrins on bone-forming cells. However, the exact mechanism of how Mn enhances osteogenesis when introduced into the surface of Ti implants is not clearly understood. This study investigated the corrosion resistance and potential osteogenic capacity of a Mn-incorporated Ti surface as determined by electrochemical measurement and examining the behaviors of human mesenchymal stem cells (MSCs) in a clinically available sandblasted/acid-etched (SLA) oral implant surface intended for future biomedical applications. The surface that resulted from wet chemical treatment exhibited the formation of a Mn-containing nanostructured TiO2 anatase thin film in the SLA implant and improved corrosion resistance. The Mn-incorporated SLA surface displayed sustained Mn ion release and enhanced osteogenesis-related MSC function, which enhanced early cellular events such as spreading, focal adhesion, and mRNA expression of critical adhesion-related genes and promoted full human MSC differentiation into mature osteoblasts. Our findings indicate that surface Mn modification by wet chemical treatment is an effective approach to produce a Ti implant surface with increased osteogenic capacity through the promotion of the osteogenic differentiation of MSCs. The improved corrosion resistance of the resultant surface is yet another important benefit of being able to provide favorable osseointegration interface stability with an increased barrier effect.

scholarly journals Titanium dioxide nanotubes increase purinergic receptor P2Y6 expression and activate its downstream PKCα–ERK1/2 pathway in bone marrow mesenchymal stem cells under osteogenic induction

2021 ◽  
Author(s):  
Chen Wang ◽  
Yanchang Liu ◽  
Xianbo Shang ◽  
Sai Ma ◽  
Huihui Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Titanium Dioxide ◽  
Osteogenic Differentiation ◽  
Purinergic Receptor ◽  
Pure Titanium ◽  
Titanium Implants ◽  
Marrow Mesenchymal Stem Cells ◽  
Osteogenic Effect

Abstract Background Titanium dioxide (TiO2) nanotubes can improve the osseointegration of pure titanium implants, but this exact mechanism has not been fully elucidated. The purinergic receptor P2Y6 is expressed in bone marrow mesenchymal stem cells (BMSCs) and participates in the regulation of bone metabolism. However, it is unclear as to whether P2Y6 is involved in the osteogenic differentiation of BMSCs induced by TiO2 nanotubes. Methods TiO2 nanotubes were prepared on the surface of titanium specimens using the anodizing method. The surface morphology of the nanotubes was observed by a scanning electron microscope, and the elemental analysis was carried out by X-ray energy dispersive spectroscopy. Quantitative reverse transcriptase polymerase chain reaction and western blotting were used to detect the expression of P2Y6, markers of osteogenic differentiation, and PKCα–ERK1/2. Results The average inner diameter of the TiO2 nanotubes increases with an increase in voltage (voltage range of 30–90V), and the expression of P2Y6 in BMSCs could be upregulated by TiO2 nanotubes in osteogenic culture. Inhibition of P2Y6 expression partially inhibited the osteogenic effect of TiO2 nanotubes and downregulated the activity of the PKCα–ERK1/2 pathway. The osteogenic effect of TiO2 nanotubes when combined with P2Y6 agonists was more pronounced. Conclusions TiO2 nanotubes can promote the P2Y6 expression of BMSCs during osteogenic differentiation and promote osteogenesis by activating the PKCα–ERK1/2 pathway.

scholarly journals Evaluation of Antimicrobial Effect of Air-Polishing Treatments and Their Influence on Human Dental Pulp Stem Cells Seeded on Titanium Disks

2021 ◽  
Vol 22 (2) ◽  
pp. 865
Author(s):  
Rosanna Di Tinco ◽  
Giulia Bertani ◽  
Alessandra Pisciotta ◽  
Laura Bertoni ◽  
Jessika Bertacchini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Early Period ◽  
Dental Pulp Stem Cells ◽  
Titanium Implants ◽  
Bacterial Biofilm ◽  
Air Polishing ◽  
Human Dental Pulp

Dental implants are one of the most frequently used treatment options for tooth replacement, and titanium is the metal of choice due to its demonstrated superiority in resisting corrosion, lack of allergic reactions and mechanical strength. Surface roughness of titanium implants favors the osseointegration process; nevertheless, its topography may provide a suitable substrate for bacterial biofilm deposition, causing peri-implantitis and leading to implant failure. Subgingival prophylaxis treatments with cleansing powders aimed to remove the bacterial accumulation are under investigation. Two different air-polishing powders—glycine and tagatose—were assayed for their cleaning and antimicrobial potential against a Pseudomonas biofilm and for their effects on human dental pulp stem cells (hDPSCs), seeded on sandblasted titanium disks. Immunofluorescence analyses were carried out to evaluate cell adhesion, proliferation, stemness and osteogenic differentiation. The results demonstrate that both the powders have a great in vitro cleaning potential in the early period and do not show any negative effects during hDPSCs osteogenic differentiation process, suggesting their suitability for enhancing the biocompatibility of titanium implants. Our data suggest that the evaluated cleansing systems reduce microbial contamination and allow us to propose tagatose as an adequate alternative to the gold standard glycine for the air-polishing prophylaxis treatment.

BMP9 Regulates Osteogenic Differentiation of Mesenchymal Stem Cells Through JNKs Kinase Pathway

2013 ◽  
Vol 40 (12) ◽  
pp. 1220
Author(s):  
Jing XU ◽  
Dan ZHAO ◽  
Jian WANG ◽  
WenJuan WANG ◽  
JinYong LUO
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Kinase Pathway

Wnt7a Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells

2019 ◽  
Author(s):  
Leiluo Yang ◽  
Qing Li ◽  
Junhong Zhang ◽  
Pengcheng Li ◽  
Chaoliang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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