scholarly journals Effect of detrusor overactivity on the expression of aquaporins and nitric oxide synthase in rat urinary bladder following bladder outlet obstruction

2013 ◽  
Vol 7 (5-6) ◽  
pp. 268 ◽  
Author(s):  
Sun-Ouck Kim ◽  
Dongjune Choi ◽  
Seung Hee Song ◽  
Kyu Youn Ahn ◽  
Dongdeuk Kwon ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Urinary Bladder ◽  
Detrusor Overactivity ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Control Group ◽  
Rat Urinary Bladder ◽  
Oxide Synthase ◽  
Nos Isoforms

Background: Aquaporins (AQPs) have recently been reported to be expressed in rat and human urothelium. Nitric oxide (NO) is thought to play a role in the bladder overactivity related to bladder outlet obstruction (BOO). The purpose of this study is to investigate the effect of BOO on the expression of AQP2-3 and nitric oxide synthase (NOS) isoforms in rat urothelium.Methods: Female Sprague-Dawley rats (230-240 g, n = 60) were divided into 2 groups. The control group (n = 30) and the partial bladder outlet obstruction (BOO) group (n = 30). After 4 weeks, we performed a urodynamic study to measure the contraction interval and contraction pressure. The expression and cellular localization of AQP2-3, endothelial nitric oxide synthase (eNOS) and neuronalnitric oxide synthase (nNOS) were determined by Western blot and immunohistochemistry.Results: On the cystometrogram, the estimated contraction interval time (minutes, mean ± SE) was significantly lower in the BOO group (3.0 ± 0.9) than in the control group (6.3 ± 0.4; p < 0.05). AQP2 was localized in the cytoplasm of the epithelium, whereas AQP3 was found only in the cell membrane of the epithelium. The protein expression of AQP2-3, eNOS and nNOS was significantly increased in the BOO group.Conclusion: Detrusor overactivity induced by BOO causes a significant increase in the expression of AQP2-3, eNOS, and nNOSin rat urinary bladder. This may imply that the AQPs and NOS isoforms have a functional role in the bladder dysfunction that occurs in association with BOO.

Evaluation of a rat model of functional urinary bladder outlet obstruction produced by chronic inhibition of nitric oxide synthase

Life Sciences ◽  
2019 ◽  
Vol 234 ◽  
pp. 116772
Author(s):  
Katsuhiko Noguchi ◽  
Kimio Sugaya ◽  
Saori Nishijima ◽  
Mayuko Sakanashi ◽  
Katsumi Kadekawa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Urinary Bladder ◽  
Rat Model ◽  
Bladder Outlet Obstruction ◽  
Outlet Obstruction ◽  
Oxide Synthase

scholarly journals Expression of Constitutive Nitric Oxide Synthase Isoforms in Varicose Vein Wall; Preliminary Results

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Zora Haviarová ◽  
Andrea Janegová ◽  
Pavel Janega ◽  
Štefan Durdík ◽  
Peter Kováč ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Varicose Vein ◽  
Structural Changes ◽  
Varicose Veins ◽  
Control Group ◽  
Color Analysis ◽  
Vein Wall ◽  
Oxide Synthase ◽  
Nos Isoforms

There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpaired -test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works.

scholarly journals β-Adrenoceptor Agonists Stimulate Endothelial Nitric Oxide Synthase in Rat Urinary Bladder Urothelial Cells

2002 ◽  
Vol 22 (18) ◽  
pp. 8063-8070 ◽  
Author(s):  
Lori A. Birder ◽  
Michele L. Nealen ◽  
Susanna Kiss ◽  
William C. de Groat ◽  
Michael J. Caterina ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Urinary Bladder ◽  
Endothelial Nitric Oxide Synthase ◽  
Urothelial Cells ◽  
Rat Urinary Bladder ◽  
Adrenoceptor Agonists ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide
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