scholarly journals Evaluation of Genotoxicity and 28-day Oral Dose Toxicity on Freeze-dried Powder of Tenebrio molitor Larvae (Yellow Mealworm)

2014 ◽  
Vol 30 (2) ◽  
pp. 121-130 ◽  
Author(s):  
So-Ri Han ◽  
Eun-Young Yun ◽  
Ji-Young Kim ◽  
Jae Sam Hwang ◽  
Eun Ju Jeong ◽  
...  
Keyword(s):  
Oral Dose ◽  
Tenebrio Molitor ◽  
Yellow Mealworm ◽  
Freeze Dried ◽  
Tenebrio Molitor Larvae

scholarly journals Replacement of Fish Meal by Defatted Yellow Mealworm (Tenebrio molitor) Larvae in Diet Improves Growth Performance and Disease Resistance in Red Seabream (Pargus major)

Animals ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 100 ◽  
Author(s):  
Atsushi Ido ◽  
Atsushi Hashizume ◽  
Takashi Ohta ◽  
Takayuki Takahashi ◽  
Chiemi Miura ◽  
...  
Keyword(s):  
Fish Meal ◽  
Tenebrio Molitor ◽  
Animal Protein ◽  
Edwardsiella Tarda ◽  
Partial Replacement ◽  
Yellow Mealworm ◽  
Complete Replacement ◽  
Red Seabream ◽  
Mealworm Larvae ◽  
Tenebrio Molitor Larvae

Yellow mealworm (Tenebrio molitor) larvae are a potential alternative animal protein source for sustainable aquaculture. However, reports on the successful complete substitution of fish meal with yellow mealworm larvae in an aquaculture diet have been limited. In this study, we conducted a feeding trial with red seabream (Pagrus major) being fed diets with partial or complete replacement of fish meal with yellow mealworm larvae defatted with a hexane–ethanol solution. Feed intake in red seabream increased in accordance with yellow mealworm larvae inclusion, and diets including 65% defatted mealworm larvae (complete replacement of fish meal) showed significant growth promotion. The addition of the oil fraction from mealworm larvae to diets resulted in growth reduction, despite meeting the nutritional requirements of red seabream. Moreover, the survival rate of red seabreams fed diets with partial replacement of fish meal with mealworm larvae was significantly higher in a challenge test with pathogenic Edwardsiella tarda bacteria. The present study demonstrated that yellow mealworm larvae are not merely an alternative animal protein, but have potential as functional feed ingredients for aquaculture production.

Digestive proteinases of yellow mealworm (Tenebrio molitor) larvae: Purification and characterization of a trypsin-like proteinase

2005 ◽  
Vol 70 (3) ◽  
pp. 300-305 ◽  
Author(s):  
T. A. Tsybina ◽  
Y. E. Dunaevsky ◽  
M. A. Belozersky ◽  
D. P. Zhuzhikov ◽  
B. Oppert ◽  
...  
Keyword(s):  
Tenebrio Molitor ◽  
Purification And Characterization ◽  
Yellow Mealworm ◽  
Digestive Proteinases ◽  
Tenebrio Molitor Larvae

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

2020 ◽  
Vol 36 (6) ◽  
pp. 78-86
Author(s):  
P.I. Akentyev ◽  
I.I. Gubaidullin ◽  
N.I. Zhiganov ◽  
V.F. Tereshchenkova ◽  
E.N. Elpidina ◽  
...  
Keyword(s):  
Culture Medium ◽  
Polypeptide Chain ◽  
Tenebrio Molitor ◽  
Enzyme Secretion ◽  
Trypsin Treatment ◽  
Yellow Mealworm ◽  
Serine Peptidase

A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast, the target His6-tagged protein was produced in two forms. One of them was a monomer that was efficiently purified by Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of incorrect folding of the polypeptide chain. Tenebrio molitor, S1 family serine peptidase, SerP38, yeast, Komagataella kurtzmanii, ion-dependent aggregation

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