scholarly journals Biochemical characterization of ferredoxin-NADP+ reductase interaction with flavodoxin in Pseudomonas putida

BMB Reports ◽  
2012 ◽  
Vol 45 (8) ◽  
pp. 476-481 ◽  
Author(s):  
Jin-Ki Yeom ◽  
Woo-Jun Park
Keyword(s):  
Pseudomonas Putida ◽  
Biochemical Characterization

Modified Sutter’s Arginine Dihydrolase Medium for Pseudomonas Speciation

1977 ◽  
Vol 60 (2) ◽  
pp. 311-312
Author(s):  
Sylvia G Cary
Keyword(s):  
Pseudomonas Putida ◽  
Biochemical Characterization ◽  
False Negative ◽  
Bromothymol Blue ◽  
Negative Reaction ◽  
Color Contrast ◽  
Pseudomonas Sp ◽  
Cosmetic Products ◽  
Arginine Dihydrolase

Abstract Sutter’s arginine dihydrolase medium has been modified to obtain maximum yields of arginine dihydrolase. Bromothymol blue is the indicator for alkalinity in Sutter’s medium. By adding glucose and lowering the pH of the medium, more positive reactions were obtained in 24 hr as well as a sharper color contrast to the base medium which facilitated reading the reactions. The modified Sutter’s medium was included in the routine biochemical schema for speciation of pseudomonads isolated from cosmetic products. Of the 706 strains belonging to the 5 species isolated from cosmetic products, only one false negative reaction was obtained with a strain of Pseudomonas putida. The medium was also used for the biochemical characterization of 122 Pseudomonas sp. isolates.

scholarly journals Molecular and biochemical characterization of Pseudomonas putida isolated from bottled uncarbonated mineral drinking water

2014 ◽  
Vol 66 (1) ◽  
pp. 23-28 ◽  
Author(s):  
S. Tasic ◽  
M. Kojic ◽  
D. Obradovic ◽  
Irena Tasic
Keyword(s):  
Drinking Water ◽  
16S Rrna ◽  
Pseudomonas Putida ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Pcr Amplification ◽  
Identification System ◽  
Rrna Gene ◽  
Identification Software

Pseudomonas putida belongs to a group of opportunistic pathogens that can cause disease in people with weakened or damaged immune systems. Some strains have medical significance, and for most ingestion is not the primary route of infection. If water used by predisposed subjects is contaminated by P. putida, they may become ill. The aim of this work was the biochemical and molecular characterization of strain ST3 of P. putida isolated from non-carbonated bottled drinking water from Jakov Do 4 on Mt. Vlasina. Characterization of P. putida was performed to assess the risk to human health of the indigenous strains present in the water. Biochemical characterization of strains was performed using the manual identification system ID 32 GN (BioM?rieux). Identification was obtained using the database identification software ATB System (Bio-M?rieux). Molecular characterization was performed by PCR amplification and 16S rDNA ?thermal cycling sequencing?. Biochemical identification of the strain ST3 was accurate (Id = 99.8%). Comparing the sequences obtained for strain ST3 with NCBI gene bank sequences for 16S rRNA, the highest similarity of our strain (96% identity) with a strain of P. putida, designated as biotype A (gi|18076625|emb|AJ308311.1|.PPU308311) isolated in New Zealand, was obtained. While comparison with the NCBI collection of all deposited sequences showed that the 16S rRNA gene sequence of strain ST3 has very high homology, it is not identical, indicating indirectly that strain ST3 is an indigenous strain. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/ABS151023125E">10.2298/ABS151023125E</a><u></b></font>

scholarly journals Corrigendum: Tasic S, Kojic M, Obradovic D, Tasic I. Molecular and biochemical characterization of Pseudomonas putida isolated from bottled uncarbonated mineral drinking water. Arch Biol Sci. 2014, 66(1):23-8. DOI:10.2298/ABS1401023T

2016 ◽  
Vol 68 (1) ◽  
pp. 239-239
Author(s):  
E Editorial
Keyword(s):  
Drinking Water ◽  
Genetic Engineering ◽  
Pseudomonas Putida ◽  
Molecular Genetics ◽  
Biomedical Engineering ◽  
Biochemical Characterization ◽  
Food Technology ◽  
Full List ◽  
Font Color

All authors of the article (Tasic Srdjan, Kojic Milan, Obradovic D., Tasic Irena) have submitted a written statement that Zorana Z. Golubovic was not included in the list of authors. With this corrigendum we are correcting this. The full list of authors for this article is as follows: Tasic Srdjan, Department of Food Technology, Higher School of Applied Professional Studies, 17500 Vranje, Serbia Kojic Milan, University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, 11010 Belgrade, Serbia Obradovic D., Institute of Food Technology and Biotechnology, Faculty of Agriculture, University of Belgrade, 11080 Zemun, Serbia Golubovic Zorana Z., Biomedical Engineering, Faculty of Mechanical Engineering, University of Belgrade, 11120 Belgrade 35, Serbia Tasic Irena, Pharmaceutical Institutie Vranje, 17500 Vranje, Serbia <br><br><font color="red"><b> Link to the corrected article <u><a href="http://dx.doi.org/10.2298/ABS1401023T">10.2298/ABS1401023T</a></b></u>

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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